Abstract:
:Extensive use of antibiotics in both public health and animal husbandry has resulted in rapid emergence of antibiotic resistance in almost all human pathogens, including biothreat pathogens. Antibiotic resistance has thus become a major concern for both public health and national security. We developed multiplexed assays for rapid, simultaneous pathogen detection and characterization of ciprofloxacin and doxycycline resistance in Bacillus anthracis, Yersinia pestis, and Francisella tularensis. These assays are SNP-based and use Multiplexed Oligonucleotide Ligation-PCR (MOL-PCR). The MOL-PCR assay chemistry and MOLigo probe design process are presented. A web-based tool - MOLigoDesigner (http://MOLigoDesigner.lanl.gov) was developed to facilitate the probe design. All probes were experimentally validated individually and in multiplexed assays, and minimal sets of multiplexed MOLigo probes were identified for simultaneous pathogen detection and antibiotic resistance characterization.
journal_name
Adv Exp Med Bioljournal_title
Advances in experimental medicine and biologyauthors
Song J,Li PE,Gans J,Vuyisich M,Deshpande A,Wolinsky M,White PSdoi
10.1007/978-1-4419-5913-3_51subject
Has Abstractpub_date
2010-01-01 00:00:00pages
455-64eissn
0065-2598issn
2214-8019journal_volume
680pub_type
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