A novel FY*A allele with the 265T and 298A SNPs formerly associated exclusively with the FY*B allele and weak Fy(b) antigen expression: implication for genotyping interpretative algorithms.

Abstract:

BACKGROUND AND OBJECTIVES:An Australian Caucasian blood donor consistently presented a serology profile for the Duffy blood group as Fy(a+b+) with Fy(a) antigen expression weaker than other examples of Fy(a+b+) red cells. Molecular typing studies were performed to investigate the reason for the observed serology profile. MATERIAL AND METHODS:Blood group genotyping was performed using a commercial SNP microarray platform. Sanger sequencing was performed using primer sets to amplify across exons 1 and 2 of the FY gene and using allele-specific primers. RESULTS:The propositus was genotyped as FY*A/B, FY*X heterozygote that predicted the Fy(a+b+(w) ) phenotype. Sequencing identified the 265T and 298A variants on the FY*A allele. This link between FY*A allele and 265T was confirmed by allele-specific PCR. CONCLUSION:The reduced Fy(a) antigen reactivity is attributed to a FY*A allele-carrying 265T and 298A variants previously defined in combination only with the FY*B allele and associated with weak Fy(b) antigen expression. This novel allele should be considered in genotyping interpretative algorithms for generating a predicted phenotype.

journal_name

Vox Sang

journal_title

Vox sanguinis

authors

Lopez GH,Condon JA,Wilson B,Martin JR,Liew YW,Flower RL,Hyland CA

doi

10.1111/vox.12185

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

52-7

issue

1

eissn

0042-9007

issn

1423-0410

journal_volume

108

pub_type

杂志文章
  • Biochemistry of whole blood in poly(ethylene-co-ethylacrylate) experimental blood containers.

    abstract::The biochemical status of whole blood stored in containers fabricated of ethylene ethylacrylate (EEA) film was monitored at several times during 4 weeks of storage at 4 degrees C. Fifteen biochemical indicators were studied to reflect on erythrocyte integrity, cellular metabolism, plasma protein stability, and microag...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1985.tb00161.x

    authors: Horowitz B,Rowe AW,Stryker MH,Van der Sande J,Waldman AA,Wong SC,Gass JD,Woods KR

    更新日期:1985-01-01 00:00:00

  • A new red blood cell cold autoantibody (anti-Me)

    abstract::A new red blood cell (RBC) cold autoantibody (anti-Me) is presented which was detected in a patient with autoimmune hemolytic anemia of 'cold type' associated with Waldenström's macroglobulinemia. The autoantibody was of the IgM (Kappa) class, had a moderate titer, and reacted with cord as well as with adult RBC. Its ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1985.tb01122.x

    authors: Salama A,Pralle H,Mueller-Eckhardt C

    更新日期:1985-01-01 00:00:00

  • Blood donors infected with the hepatitis B virus but persistently lacking antibodies to the hepatitis B core antigen.

    abstract:BACKGROUND AND OBJECTIVES:Antibodies to the core of hepatitis B virus (anti-HBc) are considered to be the best serologically reliable markers of hepatitis B virus (HBV) infection. Through a national epidemiological survey, two young and first-time blood donors, originating from HBV-endemic areas, were identified as HBV...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1046/j.1423-0410.2001.00016.x

    authors: Laperche S,Guitton C,Smilovici W,Courouce AM

    更新日期:2001-02-01 00:00:00

  • Microbiology, time course and clinical characteristics of infection in critically ill patients receiving packed red blood cell transfusion.

    abstract:BACKGROUND AND OBJECTIVES:Packed red blood cell transfusion has been associated with increased infection in a variety of critically ill patient populations. We evaluated the microbiology and time course of infection in transfused patients in the intensive care unit (ICU) as no data exist on these parameters. MATERIALS...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.2009.01134.x

    authors: Rachoin JS,Daher R,Schorr C,Milcarek B,Parrillo JE,Gerber DR

    更新日期:2009-11-01 00:00:00

  • Formation of mono(ethylhexyl)phthalate from di(ethylhexyl)phthalate in human plasma stored in PVC bags and its presence in fractionated plasma proteins.

    abstract::The formation of mono(ethylhexyl)phthalate (MEHP) from di(ethylhexyl)phthalate in human plasma stored in bags of polyvinylchloride has been studied. Substantial amounts were formed and in ten bags from 4 to 56microgram/ml were found. After 2 weeks at room temperature the concentration of MEHP had increased to values b...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1978.tb02903.x

    authors: Vessman J,Rietz G

    更新日期:1978-07-01 00:00:00

  • Donor follow-up of influenza vaccine-related multiple viral enzyme immunoassay reactivity.

    abstract::False-positive enzyme immunoassay (EIA) tests in blood donors receiving influenza vaccine were first reported in 1991. We conducted follow-up testing for 6 months of those donors with multiply reactive, but unconfirmed EIA (at least 2 positives in anti-HCV-1.0, anti-HIV-1, and anti-HTLV-I assays) with a history of rec...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1994.tb01658.x

    authors: Arnold NL,Slade BA,Jones MM,Popovsky MA

    更新日期:1994-01-01 00:00:00

  • Evaluation of a combined HIV-1/2 and HTLV-I/II assay for screening blood donors.

    abstract::A combined immunoassay for the simultaneous detection of antibodies to HIV-1/2 and HTLV-I/II (Bioelisa, Launch Diagnostics) has been evaluated to determine its suitability for routine use in blood screening. 84,222 donations were tested from 76,452 donors. One HIV- and 1 HTLV-1-positive donor were identified. The spec...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1995.tb02576.x

    authors: Flanagan P,McAlpine L,Ramskill SJ,Smith AG,Eglin R,Parry JV,Mortimer PP

    更新日期:1995-01-01 00:00:00

  • Serological characterization of murine monoclonal antibodies directed against acquired B red cells.

    abstract::Balb/c mice were immunized with acquired B red cells. Twelve clones specific for acquired B red cells were obtained from two fusions. A detailed investigation of three clones is reported here. These antibodies appear to be directed to the B-like epitope since they are inhibited by galactosamine and fail to react after...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1990.tb05017.x

    authors: Janvier D,Veaux S,Reviron M,Guignier F,Benbunan M

    更新日期:1990-01-01 00:00:00

  • Selective depression of blood group antigens associated with hereditary ovalocytosis among melanesians.

    abstract::Recessively inherited ovalocytosis in coastal Melanesians is associated with widespread, but selective, depression of blood group antigens in homozygotes, who comprise about 15% of these populations. It is suggested that a membrane anomaly exists, and that the series of depressed determinants all depend, for their ful...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1977.tb00612.x

    authors: Booth PB,Serjeantson S,Woodfield DG,Amato D

    更新日期:1977-01-01 00:00:00

  • Survival of transfused platelets collected into new formulation plastic packs.

    abstract::Platelet concentrates were prepared in plastic packs of polyvinyl chloride with tri(2-ethylhexyl) trimellitate as plasticizer. They were stored, with gentle shaking, at room temperature for periods up to 7 days before labelling with isotope and reinfusing. In vivo survival studies, platelet counts, pH and electron mic...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1982.tb00016.x

    authors: Archer GT,Grimsley PG,Jindra J,Robson JE,Ribeiro A

    更新日期:1982-01-01 00:00:00

  • Cytomegalovirus (CMV) antibody in male homosexuals: a source for CMV immune globulin.

    abstract::Male homosexuals have been found to have an extraordinarily high prevalence, and high titers, of antibodies to cytomegalovirus (CMV), as determined by complement-fixation and passive hemagglutination tests. These findings support other data suggesting that CMV may be sexually transmitted. The high titers of CMV antibo...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1983.tb01881.x

    authors: Ikram H,Prince AM,Baker LN

    更新日期:1983-03-01 00:00:00

  • Benefits and complications of regular blood transfusion in patients with beta-thalassaemia major.

    abstract::Early and regular blood transfusion therapy in patients with homozygous beta-thalassaemia decreases the complications of severe anaemia and prolongs survival. In the long term, however, the beneficial effects of transfusions are limited by the organ damage resulting from iron overload, a consequence of the body's limi...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1159/000031230

    authors: Prati D

    更新日期:2000-01-01 00:00:00

  • A multicentre evaluation of a new filtration protocol for leucocyte depletion of high-haematocrit red blood cells collected by an automated blood collection system.

    abstract:BACKGROUND AND OBJECTIVES:A multicentre trial was set up to evaluate the performance of a new leucodepletion protocol. MATERIALS AND METHODS:Filtration at high haematocrit was started during collection of red blood cell (RBC) products by apheresis with Trima. SAG-M was added after filtration through the filter. Haemat...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章,多中心研究

    doi:10.1046/j.1423-0410.2001.00078.x

    authors: Soli M,Blanco L,Riggert J,Martínez-Clavel A,Lucas C,Lunghi M,Belloni M,Wolf C,van Waeg G,Antoon M

    更新日期:2001-01-01 00:00:00

  • A microtiter modification of granulocyte immunofluorescence.

    abstract::The granulocyte immunofluorescence test (GIFT) is valuable for detecting allogeneic and autologous granulocyte antibodies. However, the original tube technique (macro GIFT) requires 50-100 microliters of serum and 10(6) granulocytes with time-consuming washing steps which cause cell loss. We report a modification (mic...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1985.tb00777.x

    authors: Press C,Kline WE,Clay ME,McCullough J

    更新日期:1985-01-01 00:00:00

  • Peroxidative events in stored platelet concentrates.

    abstract::Changes in metabolic and functional activity of platelets stored as platelet concentrates in plastic bags highly permeable to gases were investigated. The following parameters were measured daily: pH, pO2, pCO2, HCO3, glucose, lactic acid, lactic dehydrogenase, cellular ATP and platelet aggregation induced by differen...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1989.tb03043.x

    authors: Fagiolo E,Lippa S,Mores N,Oradei A,Aureli V

    更新日期:1989-01-01 00:00:00

  • Pilot study for large-scale plasma procurement using automated plasmapheresis.

    abstract::A pilot study for large-scale automated plasmapheresis using the Haemonetics Model 50 machine was undertaken in the Yorkshire Region of the United Kingdom to determine the viability of such a programme for national self-sufficiency in fresh plasma procurement for factor VIII concentrate production. The study was desig...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1983.tb01876.x

    authors: Robinson AE,Penny AF,Smith J,Tovey DL

    更新日期:1983-03-01 00:00:00

  • Reticulocyte count in red-blood-cell units stored in AS-1.

    abstract:BACKGROUND AND OBJECTIVES:Previous data that showed maintenance of reticulocyte percentage in whole blood stored in CPDA-1 have led to the assumption that reticulocyte maturation becomes arrested during refrigerated storage. However, reticulocyte behaviour in red-blood-cell units stored in additive solutions has not ye...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12011

    authors: Urbina A,Palomino F

    更新日期:2013-05-01 00:00:00

  • The MNSs antigen Ridley (Ria).

    abstract::After more than 20 years the extended pedigree of the only known kindred carrying the low frequency antigen Ria (Ridley) was reinvestigated. It is established that Ria is governed by a gene which is part of or very closely linked to the MNSs locus. Further serological and frequency studies are reported. ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1984.tb00100.x

    authors: Contreras M,Armitage SE,Stebbing B

    更新日期:1984-01-01 00:00:00

  • A new method to collect granulocytes using a low dose of hydroxyethyl starch.

    abstract::A new granulocyte collection method employing discontinuous flow centrifugation and low donor dose of hydroxyethyl starch (HES) is presented. Following the procedure, the increase in serum polysaccharide concentration was 100 mg/dl, about 20% of that seen after standard discontinuous flow leukapheresis. The average ef...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1983.tb04111.x

    authors: Szymanski IO

    更新日期:1983-01-01 00:00:00

  • T-cell subsets in autologous and allogeneic peripheral blood stem cell concentrates.

    abstract:BACKGROUND AND OBJECTIVES:Regulatory T cells (Tregs) and other T-cell subsets are of importance in the setting of autologous and allogeneic stem cell transplantations. We conducted a study to assess the content of peripheral blood stem cell concentrates and related apheresis parameters in the autologous and allogeneic ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12289

    authors: Strobel J,Moellmer I,Zingsem J,Hauck-Dlimi B,Eckstein R,Strasser E

    更新日期:2015-11-01 00:00:00

  • Recurrent hepatitis caused by two viruses acquired simultaneously during blood transfusion.

    abstract::We here report 2 patients who have acquired two consecutive episodes each of acute hepatitis, within 6 months after blood transfusion. The clinical and biochemical course was typical of recurrent acute viral hepatitis, and the patients recovered completely after each episode. The first infection was probably caused by...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1980.tb01859.x

    authors: Ben-Dov I,Fainaru M

    更新日期:1980-10-01 00:00:00

  • Prospective evaluation of immune haemolysis in liver transplantation.

    abstract:BACKGROUND:Immune haemolysis in liver transplant (LT) can occur due to autoantibodies and alloantibodies. The aim of this study was to evaluate the prevalence and risk factors for immune haemolysis in LT. METHODS:A total of 175 consecutive patients were included. Multiorgan recipients were excluded. Samples, from befo...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12865

    authors: Brunetta DM,de Barros Carlos LM,Da Silva VFP,de Oliveira Alves TM,Macedo ÊS,Coelho GR,Vasconcelos JBM,De Francesco Daher E,Garcia JHP

    更新日期:2020-01-01 00:00:00

  • An analysis of autologous blood donor motivational factors.

    abstract::Little is known about the patient's reasons for participating in an autologous blood (AUB) collection program. We surveyed 110 AUB donors in our hospital-based collection program. Although fear of infection from an allogeneic blood transfusion was cited by 20% of AUB donors, 68.2% indicated that their physician's reco...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1995.tb01679.x

    authors: Domen RE,Ribicki LA,Hoeltge GA

    更新日期:1995-01-01 00:00:00

  • A detailed serological study of five anti-Jka sera reacting by the antiglobulin technique.

    abstract::Detailed serological investigation of five examples of anti-Jka revealed considerable heterogeneity in their characteristics. All the sera were investigated by the antiglobulin technique, with and without complement and by a range of sensitization and testing methods. Three were readily detected in the presence of com...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1983.tb01897.x

    authors: Howell P,Giles CM

    更新日期:1983-01-01 00:00:00

  • Clinical tolerance of methylene blue virus-inactivated plasma. A randomized crossover trial in 12 healthy human volunteers.

    abstract:OBJECTIVES:Baxter's methylene blue (MB) photoinactivation of fresh frozen plasma (FFP) is a method that effectively removes any intracellular potentially infectious agent, such as prions, by filtration combined with an improved photochemical virus inactivation on a single unit of FFP. The purpose of this study was to d...

    journal_title:Vox sanguinis

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1159/000031129

    authors: Simonsen AC,Sørensen H

    更新日期:1999-01-01 00:00:00

  • Azapropazone-associated antibodies.

    abstract::2 patients on the drug azapropazone developed positive antiglobulin tests. Their sera were each found to contain two distinct antibodies. One was an IgG antibody, probably a drug-dependent autoantibody of the alpha-methyldopa type, but could have been related to the disease. The other was certainly a drug-dependent an...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1984.tb00095.x

    authors: Bird GW,Wingham J,Babb RG,Bacon P,Wood D

    更新日期:1984-01-01 00:00:00

  • Partial separation of blood clotting factors, albumin, and IgG by continuous free film electrophoresis.

    abstract::Blood plasma and factor VIII concentrate have been fractionated by continuous free film electrophoresis in an apparatus which was designed to overcome the problems of overheating usually inherent in this technique. Results show that complete separation of plasma proteins is unlikely to be obtained by a single passage ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1983.tb01877.x

    authors: Austen DE,Cartwright T,Dickerson CH

    更新日期:1983-03-01 00:00:00

  • Interactions between science, government and media on selection and testing of donors.

    abstract::The international media attention to "bad blood" has fueled governmental action in countries around the world. Donor selection criteria continue to expand, focusing most recently on the possibility of exclusion due to intranasal cocaine use and, in the US, due to incarceration for more than 72 hours. As newer methods ...

    journal_title:Vox sanguinis

    pub_type: 杂志文章,评审

    doi:10.1111/j.1423-0410.1998.tb05465.x

    authors: Sazama K

    更新日期:1998-01-01 00:00:00

  • Maximising platelet availability by delaying cold storage.

    abstract:BACKGROUND AND OBJECTIVES:Cold-stored platelets may be an alternative to conventional room temperature (RT) storage. However, cold-stored platelets are cleared more rapidly from circulation, reducing their suitability for prophylactic transfusion. To minimise wastage, it may be beneficial to store platelets conventiona...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/vox.12649

    authors: Wood B,Johnson L,Hyland RA,Marks DC

    更新日期:2018-04-06 00:00:00

  • Standardization of bromelin for use in routine one-stage antibody screening.

    abstract::A simple standardization method for bromelin used in routine one-stage antibody screening is described. Bromelin proteinase activity was assayed using casein as the substrate, and converted to units. The use of proteinase activity units for standardization of bromelin resolves differences between commercial preparatio...

    journal_title:Vox sanguinis

    pub_type: 杂志文章

    doi:10.1111/j.1423-0410.1987.tb02991.x

    authors: Mazda T,Ogasawara K,Nakata K,Shimizu M

    更新日期:1987-01-01 00:00:00