Abstract:
:Bacteria can form heterogeneous populations containing phenotypic variants of genetically identical cells. The heterogeneity of populations can be considered a bet-hedging strategy allowing adaptation to unknown environmental changes - at least some individual subpopulations or cells might be able to withstand future adverse conditions. Using Percoll gradient centrifugation, we demonstrated that in an Escherichia coli culture exposed to heat shock at 50 °C, two physiologically distinct subpopulations were formed. A high-density subpopulation (HD50) demonstrated continued growth immediately after its transfer to LB medium, whereas the growth of a low-density subpopulation (LD50) was considerably postponed. The LD50 subpopulation contained mainly viable but non-culturable bacteria and exhibited higher tolerance to sublethal concentrations of antibiotics or H2O2 than HD50 cells. The levels of aggregated proteins and main molecular chaperones were comparable in both subpopulations; however, a decreased number of ribosomes and a significant increase in protein oxidation were observed in the LD50 subpopulation as compared with the HD50 subpopulation. Interestingly, under anaerobic heat stress, the formation of the HD50 subpopulation was decreased and culturability of the LD50 subpopulation was significantly increased. In both subpopulations the level of protein aggregates formed under anaerobic and aerobic heat stress was comparable. We concluded that the formation of protein aggregates was independent of oxidative damage induced by heat stress, and that oxidative stress and not protein aggregation limited growth and caused loss of LD50 culturability. Our results indicate that heat stress induces the formation of distinct subpopulations differing in their ability to grow under standard and stress conditions.
journal_name
Microbiol Resjournal_title
Microbiological researchauthors
Bruhn-Olszewska B,Szczepaniak P,Matuszewska E,Kuczyńska-Wiśnik D,Stojowska-Swędrzyńska K,Moruno Algara M,Laskowska Edoi
10.1016/j.micres.2018.02.002subject
Has Abstractpub_date
2018-04-01 00:00:00pages
33-42eissn
0944-5013issn
1618-0623pii
S0944-5013(17)31058-3journal_volume
209pub_type
杂志文章abstract::Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) expressing Panton-Valentine leukocidin (PVL) have been characterized worldwide, but it has not been done in Egypt. In this study, we analyzed the molecular characteristics of PVL+CA-MRSA in Egypt, compared their genetic patterns with that of PVL+...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2009.03.005
更新日期:2010-02-28 00:00:00
abstract::In search of the optimal way to reduce the hazards of environmental contamination by formaldehyde (FD) and methanol the use of unconventional yeasts is proposed as exemplified by the methylotrophic yeast Hansenula polymorpha. In a very simplified environment of a model wastewater solution, H. polymorpha cells were abl...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/S0944-5013(00)80002-6
更新日期:2000-03-01 00:00:00
abstract::Alternaria species have been recognized as important cause of allergic diseases and are considered opportunistic pathogens for immunocompromised individuals. In order to assess the contribution of waters to the spread of Alternaria, we examined 390 water samples of various origin for the presence of these microorganis...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/S0944-5013(00)80022-1
更新日期:2000-04-01 00:00:00
abstract::There are a lot of bacterial and eukaryotic DNA repair gene homologs among sequenced archaeal genomes but there is little information about DNA repair mechanisms and the interaction of involved repair proteins. In order to study DNA repair mechanisms in the third domain of life, we studied these processes in the model...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2015.01.012
更新日期:2015-04-01 00:00:00
abstract::Long-term continuous cropping has led to epidemic of bacterial wilt disease in Southern China. Bacterial wilt disease is caused by Ralstonia solanacearum and difficult to control. In order to control bacterial wilt, rice hull biochar was applied to soil with different doses (0, 7.5, 15, 30 and 45 t ha-1) in a field tr...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2019.126373
更新日期:2020-01-01 00:00:00
abstract::Molecular mechanisms underlying phytoplasma interactions with host plants are largely unknown. In this study attempts were made to identify effectors of three phytoplasma strains related to 'Ca. P. aurantifolia', crotalaria phyllody (CrP), faba bean phyllody (FBP), and witches' broom disease of lime (WBDL), using info...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2017.03.001
更新日期:2017-06-01 00:00:00
abstract::A marine Streptomyces sp. SNG9 was characterized by its ability to utilize poly(3-hydroxybutyrate) (PHB) and its copolymer poly(3-hydroxybutyrate-co-3-hydroxyvalerate P (3HB-co-HV). The bacterium grew efficiently in a simple mineral liquid medium enriched with 0.1 % poly(3-hydroxybutyrate) powder as the sole carbon so...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1078/0944-5013-00115
更新日期:2001-01-01 00:00:00
abstract::Signal recognition particle (SRP) mediates targeting of proteins to appropriate cellular compartments, which is an important process in all living organisms. In prokaryotes, SRP consists of Ffh, a protein, and 4.5S RNA that recognizes signal peptide emerging from ribosomes. The SRP (Ffh) of one the most successful int...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2012.03.002
更新日期:2012-10-12 00:00:00
abstract::Shewanella putrefaciens was grown on a series of ten alternate compounds as sole terminal electron acceptor. Each cell type was analyzed for Fe(III) reduction activity, absorbance maxima in reduced-minus-oxidized difference spectra and heme-containing protein content. High-rate Fe(III) reduction activity, pronounced d...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/S0944-5013(00)80042-7
更新日期:2000-07-01 00:00:00
abstract::Prolonged cold storage of raw milks favors the growth of psychrotrophs, which produce heat-resistant exoenzymes of considerable spoilage potential; the bacterial proteases and lipases affect raw milk quality; among them phospholipases (PLs) may target the milk fat globule. More importantly, bacterial PLs are key virul...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2008.07.001
更新日期:2010-01-01 00:00:00
abstract::Soybean (Glycine max L.) is among the most economically important legumes that provide more than 1/4 of food (for man) and animal feed. However, its yield is comparatively low, most especially under drought stress. The aim of this study therefore was to assess the ability of Rhizobium spp. and mycorrhizal fungi to enh...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126640
更新日期:2021-01-01 00:00:00
abstract::A polygalacturonase with a molecular mass of 74 kDa, an isoelectric point around pH 4.2 and pH--and temperature optima of 3.9 and 50 degrees C, respectively, was purified from a culture fluid of Penicillium frequentans. The enzyme was characterized as an exo-alpha-1,4-polygalacturonase (exo-PG I). Km and Vmax for sodi...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1078/0944-5013-00127
更新日期:2002-01-01 00:00:00
abstract::Tannase producing fungal strains were isolated from different locations including garbages, forests and orchards, etc. The strain giving maximum enzyme yield was identified to be Aspergillus ruber. Enzyme production was studied under solid state fermentation using different tannin rich substrates like ber leaves (Zyzy...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2006.06.012
更新日期:2007-01-01 00:00:00
abstract::Metal tolerance has been found to vary among Acidithiobacillus ferrooxidans strains and this can impact the efficiency of biomining practices. To explain observed strain variability for differences in metal tolerance we examined the effects of Cu(2+) and Ni(2+) concentrations (1-200 mM) on cytoplasmic membrane propert...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2010.03.004
更新日期:2011-03-20 00:00:00
abstract::Vibrio vulnificus is a serious bacterial pathogen for humans and aquatic animals. We developed a rapid, sensitive and specific identification method for V. vulnificus using loop-mediated isothermal amplification (LAMP) technique. A set of primers, composed of two outer primers and two inner primers, was designed based...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2008.05.002
更新日期:2009-01-01 00:00:00
abstract::The survivability of Salmonella cells in popcorn preparation was determined for two distinct cooking methods. The first method used a standard microwave oven. The second method used conventional cooking in a pan. Prior to thermal processing in independent experiments, 12 suspensions in a range between 1x10(3) and 8x10...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2006.03.010
更新日期:2008-01-01 00:00:00
abstract::Apoptosis stimulated by bacterial toxins is common during infection and is now considered important in disease processes. As a major human pathogen, Staphylococcus aureus also causes apoptosis during infection. In some diseases such as atopic dermatitis and sepsis, the apoptosis induced by S. aureus influences the sev...
journal_title:Microbiological research
pub_type: 杂志文章,评审
doi:10.1016/j.micres.2017.08.006
更新日期:2017-12-01 00:00:00
abstract::Actinomycetes are virtually unlimited sources of novel compounds with many therapeutic applications and hold a prominent position due to their diversity and proven ability to produce novel bioactive compounds. There are more than 22,000 known microbial secondary metabolites, 70% of which are produced by actinomycetes,...
journal_title:Microbiological research
pub_type: 杂志文章,评审
doi:10.1016/j.micres.2012.06.005
更新日期:2012-12-20 00:00:00
abstract::β-lactamase inhibitors are potent synergistic drugs to deteriorate the multidrug-resistant bacteria. Here, we report the β-lactamase inhibitory ability of kalafungin isolated from a marine sponge derived Streptomyces sp. SBRK1. The IC50 value of the kalafungin was calculated as 225.37 ± 1.95 μM against β-lactamase. Th...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126666
更新日期:2021-03-01 00:00:00
abstract::Forty-one Lactobacillus strains were tested for antagonistic activity against nine strains of Vibrio. L. plantarum and L. casei were the most effective, and L. brevis was the least effective in inhibiting the growth of Vibrio species. L. gasseri and L. helveticus strains showed higher activity, while L. reuteri and L....
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/s0944-5013(98)80011-6
更新日期:1998-11-01 00:00:00
abstract::The orphan histidine kinase (HK) from Streptomyces peucetius ATCC 27952 (ohkAsp) was found to be implicated in the regulation of doxorubicin (DOX)/daunorubicin (DNR) biosynthesis, self-defense and developmental attributes. OhkAsp is a homolog of OhkA from Streptomyces coelicolor and Streptomyces avermitilis (with 73 a...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2018.05.007
更新日期:2018-09-01 00:00:00
abstract::To evaluate the roles of blaIMP and blaTEM genes in the resistance of Serratia marcescens against beta-lactams and to find the spreading ways of these genes, 19 clinical isolates of imipenem-resistant Serratia marcescens were analyzed. Six strains bore blaIMP and blaTEM genes on a single plasmid, as confirmed by trans...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2006.06.005
更新日期:2007-01-01 00:00:00
abstract::For a long a time programmed cell death was thought to be a unique characteristic of higher eukaryotes, but evidence has accumulated showing that programmed cell death is a universal phenomenon in all life forms. Many different types of bacterial programmed cell death systems have been identified, rivalling the eukary...
journal_title:Microbiological research
pub_type: 杂志文章,评审
doi:10.1016/j.micres.2017.11.016
更新日期:2018-03-01 00:00:00
abstract::The operation of wastewater treatment technologies depends on a combination of physical, chemical and biological factors. Microorganisms present in wastewater treatment plants play essential roles in the degradation and removal of organic waste and xenobiotic pollutants. Several microorganisms have been used in comple...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2016.08.006
更新日期:2016-11-01 00:00:00
abstract::Among the 49 strains of moderately halophilic bacteria isolated from the salty environments of Iran, a Gram-positive coccus designated as strain QW6 showed high capacity in the removal of toxic oxyanions of tellurium in a wide range of culture medium factors including pH (5.5-10.5), temperature (25-45 degrees C), vari...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2006.07.010
更新日期:2008-01-01 00:00:00
abstract::The classical salicylaldehyde dehydrogenases found in naphthalene-degrading bacteria are denoted as NahF. In addition to NahF, NahV, and its corresponding gene nahV, were found here in multiple naphthalene-degrading bacteria isolated from industrial wastewater polluted with polycyclic aromatic hydrocarbons (PAHs). In ...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2011.01.003
更新日期:2011-12-20 00:00:00
abstract::Endophytic bacteria are the plant beneficial bacteria that thrive inside plants and can improve plant growth under normal and challenging conditions. They can benefit host plants directly by improving plant nutrient uptake and by modulating growth and stress related phytohormones. Indirectly, endophytic bacteria can i...
journal_title:Microbiological research
pub_type: 杂志文章,评审
doi:10.1016/j.micres.2019.02.001
更新日期:2019-04-01 00:00:00
abstract::Human interferon alpha2b (IFN-alpha2b) is a pleiotropic cytokine used for the treatment of various cancers. Antibacterial peptide CM4 is a small peptide that can strongly inhibit many kinds of bacteria, fungi, and tumor cells, but it does no harm to normal cells. Here, we describe a protein expression system for the p...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2009.01.002
更新日期:2010-02-28 00:00:00
abstract::The pathogenicity of a new China variety of Metarhizium anisopliae (M. anisopliae var. dcjhyium) against the subterranean termite Odontotermes formosanus and the effect of the fungal fermented solution on hemolymph intracellular calcium were studied in laboratory. Conidia from the M. anisopliae var. dcjhyium were high...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2006.11.009
更新日期:2009-01-01 00:00:00
abstract::The endophytic bacteria were isolated from coffee roots and seeds in Vietnam and identified with 16S rDNA sequencing as belonging to the Actinobacteria, Firmicutes and Proteobacteria phyla with the Nocardia, Bacillus and Burkholderia as dominant genera, respectively. Out of the thirty genera recovered from Coffea cane...
journal_title:Microbiological research
pub_type: 杂志文章
doi:10.1016/j.micres.2020.126613
更新日期:2021-01-01 00:00:00