Abstract:
BACKGROUND:Nucleolar rDNA is tightly associated with silent heterochromatin, which is important for rDNA stability, nucleolar integration, and cellular senescence. Two pathways have been described that lead to rDNA silencing in yeast: 1) the RENT (regulator of nucleolar silencing and telophase exit) complex, which is composed of Net1, Sir2, and Cdc14 and is required for Sir2-dependent rDNA silencing; and 2) the Sir2-independent silencing mechanism, which involves the Tof2 and Tof2-copurified complex, made up of Lrs4 and Csm1. Here, we present evidence that changes in histone H3 lysine methylation levels distinctly regulate rDNA silencing by recruiting different silencing proteins to rDNA, thereby contributing to rDNA silencing and nucleolar organization in yeast. RESULTS:We found that Lys4, Lys79, and Lys36 methylation within histone H3 acts as a bivalent marker for the regulation of rDNA recombination and RENT complex-mediated rDNA silencing, both of which are Sir2-dependent pathways. By contrast, we found that Jhd2, an evolutionarily conserved JARID1 family H3 Lys4 demethylase, effects all states of methylated H3K4 within the NTS regions of rDNA and that its activity is required for the regulation of rDNA silencing in a Sir2-independent manner. In this context, Jhd2 regulates rDNA recombination through the Tof2/Csm1/Lrs4 pathway and prevents excessive recruitment of Tof2, Csm1/Lrs4 and condensin subunits to the replication fork barrier (RFB) site within the NTS1 region. Our FISH analyses further demonstrate that the demethylase activity of Jhd2 regulates mitotic rDNA condensation and that JHD2-deficient cells contain the mostly hypercondensed rDNA mislocalized away from the nuclear periphery. CONCLUSIONS:Our results show that yeast Jhd2, which demethylates histone H3 Lys4 near the rDNA locus, regulates rDNA repeat stability and rDNA silencing in a Sir2-independent manner by maintaining Csm1/Lrs4 and condensin association with rDNA regions during mitosis. These data suggest that Jhd2-mediated alleviation of excessive Csm1/Lrs4 or condensin at the NTS1 region of rDNA is required for the integrity of rDNA repeats and proper rDNA silencing during mitosis.
journal_name
BMC Bioljournal_title
BMC biologyauthors
Ryu HY,Ahn Sdoi
10.1186/s12915-014-0075-3subject
Has Abstractpub_date
2014-09-24 00:00:00pages
75issn
1741-7007pii
s12915-014-0075-3journal_volume
12pub_type
杂志文章相关文献
BMC BIOLOGY文献大全abstract:BACKGROUND:Collective neural crest cell migration is critical to the form and function of the vertebrate face and neck, distributing bone, cartilage, and nerve cells into peripheral targets that are intimately linked with head vasculature. The vasculature and neural crest structures are ultimately linked, but when and ...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-016-0323-9
更新日期:2016-12-15 00:00:00
abstract::The visual pathway is tasked with processing incoming signals from the retina and converting this information into adaptive behavior. Recent studies of the larval zebrafish tectum have begun to clarify how the 'micro-circuitry' of this highly organized midbrain structure filters visual input, which arrives in the supe...
journal_title:BMC biology
pub_type: 杂志文章,评审
doi:10.1186/1741-7007-8-126
更新日期:2010-09-28 00:00:00
abstract:BACKGROUND:Membrane proteins form key nodes in mediating the cell's interaction with the surroundings, which is one of the main reasons why the majority of drug targets are membrane proteins. RESULTS:Here we mined the human proteome and identified the membrane proteome subset using three prediction tools for alpha-hel...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-7-50
更新日期:2009-08-13 00:00:00
abstract:BACKGROUND:Insects helped pioneer, and persist as model organisms for, the study of specific aspects of immunity. Although they lack an adaptive immune system, insects possess an innate immune system that recognizes and destroys intruding microorganisms. Its operation under natural conditions has not been well studied,...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-5-56
更新日期:2007-12-21 00:00:00
abstract:BACKGROUND:Increasing evidence suggests that influenza reassortment not only contributes to the emergence of new human pandemics but also plays an important role in seasonal influenza epidemics, disease severity, evolution, and vaccine efficacy. We studied this process within 2091 H3N2 full genomes utilizing a combinat...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-016-0337-3
更新日期:2016-12-29 00:00:00
abstract::The expansion of genome sequencing projects has produced accumulating evidence for lateral transfer of genes between prokaryotic and eukaryotic genomes. However, it remains controversial whether these genes are of functional importance in their recipient host. Nikoh and Nakabachi, in a recent paper in BMC Biology, tak...
journal_title:BMC biology
pub_type: 评论,杂志文章
doi:10.1186/1741-7007-7-20
更新日期:2009-05-05 00:00:00
abstract:BACKGROUND:The next big challenge in human genetics is understanding the 98% of the genome that comprises non-coding DNA. Hidden in this DNA are sequences critical for gene regulation, and new experimental strategies are needed to understand the functional role of gene-regulation sequences in health and disease. In thi...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-11-106
更新日期:2013-10-14 00:00:00
abstract:BACKGROUND:Parkinson's disease (PD) is a progressive neurodegenerative disorder characterized by both motor and non-motor symptoms. Gastrointestinal tract dysfunction is one of the non-motor features, where constipation is reported as the most common gastrointestinal symptom. Aromatic bacterial metabolites are attracti...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-020-00876-3
更新日期:2020-10-20 00:00:00
abstract:BACKGROUND:The availability of abundant sequence data from key model organisms has made large scale studies of molecular evolution an exciting possibility. Here we use full length cDNA alignments comprising more than 700,000 nucleotides from human, mouse, pig and the Japanese pufferfish Fugu rubrices in order to invest...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-3-2
更新日期:2005-01-28 00:00:00
abstract::The new field of viral dynamics, based on within-host modeling of viral infections, began with models of human immunodeficiency virus (HIV), but now includes many viral infections. Here we review developments in HIV modeling, emphasizing quantitative findings about HIV biology uncovered by studying acute infection, th...
journal_title:BMC biology
pub_type: 杂志文章,评审
doi:10.1186/1741-7007-11-96
更新日期:2013-09-03 00:00:00
abstract:BACKGROUND:Effective disease management depends on timely and accurate diagnosis to guide control measures. The capacity to distinguish between individuals in a pathogen population with specific properties such as fungicide resistance, toxin production and virulence profiles is often essential to inform disease managem...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-019-0684-y
更新日期:2019-08-13 00:00:00
abstract::A recent paper published in BMC Genomics suggests that retrotransposition may be active in the human gut parasite Entamoeba histolytica. This adds to our knowledge of the various types of repetitive elements in parasitic protists and the potential influence of such elements on pathogenicity. ...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-8-64
更新日期:2010-05-24 00:00:00
abstract::Malaria is a common and life-threatening disease endemic in large parts of the world. The emergence of antimalarial drug resistance is threatening disease-control measures that depend heavily on treatment of clinical malaria. The intracellular malaria parasite is particularly vulnerable during its brief extracellular ...
journal_title:BMC biology
pub_type: 评论,杂志文章
doi:10.1186/s12915-015-0185-6
更新日期:2015-09-08 00:00:00
abstract:BACKGROUND:Metazoan lineages exhibit a wide range of regenerative capabilities that vary among developmental stage and tissue type. The most robust regenerative abilities are apparent in the phyla Cnidaria, Platyhelminthes, and Echinodermata, whose members are capable of whole-body regeneration (WBR). This phenomenon h...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-019-0633-9
更新日期:2019-02-22 00:00:00
abstract:BACKGROUND:The advent of next generation sequencing (NGS) has allowed the discovery of short and long non-coding RNAs (ncRNAs) in an unbiased manner using reverse genetics approaches, enabling the discovery of multiple categories of ncRNAs and characterization of the way their expression is regulated. We previously sho...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-020-0763-0
更新日期:2020-04-13 00:00:00
abstract:BACKGROUND:A key event in plant morphogenesis is the establishment of a division plane. A plant-specific microtubular preprophase band (PPB) accurately predicts the line of cell division, whereas the phragmoplast, another plant-specific array, executes cell division by maintaining this predicted line. Although establis...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-3-11
更新日期:2005-04-14 00:00:00
abstract:BACKGROUND:Management of blood cholesterol is a major focus of efforts to prevent cardiovascular diseases. The objective of this study was to investigate how the gut microbiota affects host cholesterol homeostasis at the organism scale. RESULTS:We depleted the intestinal microbiota of hypercholesterolemic female Apoe-...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-019-0715-8
更新日期:2019-11-27 00:00:00
abstract:BACKGROUND:Accurate determination of mouse positions from video data is crucial for various types of behavioral analyses. While detection of body positions is straightforward, the correct identification of nose positions, usually more informative, is far more challenging. The difficulty is largely due to variability in...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-017-0377-3
更新日期:2017-05-15 00:00:00
abstract::The efficient production, folding, and secretion of proteins is critical for cancer cell survival. However, cancer cells thrive under stress conditions that damage proteins, so many cancer cells overexpress molecular chaperones that facilitate protein folding and target misfolded proteins for degradation via the ubiqu...
journal_title:BMC biology
pub_type: 杂志文章,评审
doi:10.1186/s12915-017-0449-4
更新日期:2017-11-16 00:00:00
abstract:BACKGROUND:The most commonly occurring cancer mutations, including oncogenes such as MYC, Ras and PIK3C, are found in signal transductions pathways feeding into the translational machinery. A broad range of translation initiation factors are also commonly found to be either amplified or mis-regulated in tumours, includ...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-019-0658-0
更新日期:2019-05-22 00:00:00
abstract:BACKGROUND:Sec8 is highly expressed in mammalian nervous systems and has been proposed to play a role in several aspects of neural development and function, including neurite outgrowth, calcium-dependent neurotransmitter secretion, trafficking of ionotropic glutamate receptors and regulation of neuronal microtubule ass...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-3-27
更新日期:2005-12-13 00:00:00
abstract::Mitochondria are highly dynamic and structurally complex organelles that provide multiple essential metabolic functions. Mitochondrial dysfunction is associated with neurodegenerative conditions such as Parkinson's disease, as well as bacterial infection. Here, we explore the roles of mitochondrial autophagy (mitophag...
journal_title:BMC biology
pub_type: 杂志文章,评审
doi:10.1186/s12915-015-0129-1
更新日期:2015-04-03 00:00:00
abstract:BACKGROUND:The International Mouse Phenotyping Consortium is generating null allele mice for every protein-coding gene in the genome and characterizing these mice to identify gene-phenotype associations. While CRISPR/Cas9-mediated null allele production in mice is highly efficient, generation of conditional alleles has...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-018-0529-0
更新日期:2018-06-21 00:00:00
abstract:BACKGROUND:Seed plants are composed of angiosperms and gymnosperms, which diverged from each other around 300 million years ago. While much light has been shed on the mechanisms and rate of genome evolution in flowering plants, such knowledge remains conspicuously meagre for the gymnosperms. Conifers are key representa...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-10-84
更新日期:2012-10-26 00:00:00
abstract:BACKGROUND:The western flower thrips, Frankliniella occidentalis (Pergande), is a globally invasive pest and plant virus vector on a wide array of food, fiber, and ornamental crops. The underlying genetic mechanisms of the processes governing thrips pest and vector biology, feeding behaviors, ecology, and insecticide r...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-020-00862-9
更新日期:2020-10-19 00:00:00
abstract:BACKGROUND:Application of apomixis, or asexual seed formation, in crop breeding would allow rapid fixation of complex traits, economizing improved crop delivery. Identification of apomixis genes is confounded by the polyploid nature, high genome complexity and lack of genomic sequence integration with reproductive tiss...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/s12915-016-0311-0
更新日期:2016-10-06 00:00:00
abstract:BACKGROUND:Analyzing and understanding the relationship between genotypes and phenotypes is at the heart of genetics. Research on the nematode Caenorhabditis elegans has been instrumental for unraveling genotype-phenotype relations, and has important implications for understanding the biology of mammals, but almost all...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-11-93
更新日期:2013-08-19 00:00:00
abstract::A recent report in BMC Biology on the discovery and analysis of biosynthetic genes for ribosomal peptide natural products confirms that these pathways are much more common and diverse than previously suspected, contributing substantially to the chemical arsenal employed by bacteria. See research article http://www.bio...
journal_title:BMC biology
pub_type: 评论,杂志文章
doi:10.1186/1741-7007-8-83
更新日期:2010-06-17 00:00:00
abstract:BACKGROUND:Although a variety of animals have been used to produce polyclonal antibodies against antigens, the production of antigen-specific monoclonal antibodies from animals remains challenging. RESULTS:We propose a simple and rapid strategy to produce monoclonal antibodies from a variety of animals. By staining ly...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-10-80
更新日期:2012-09-28 00:00:00
abstract:BACKGROUND:Rickettsia are intracellular symbionts of eukaryotes that are best known for infecting and causing serious diseases in humans and other mammals. All known vertebrate-associated Rickettsia are vectored by arthropods as part of their life-cycle, and many other Rickettsia are found exclusively in arthropods wit...
journal_title:BMC biology
pub_type: 杂志文章
doi:10.1186/1741-7007-7-6
更新日期:2009-02-02 00:00:00