Abstract:
:Pretreatment of confluent human fibroblast cultures for two days in dexamethasone, serum free medium increased 10-20 fold the sensitivity of the cells to insulin-like growth factor I (IGF I) stimulation of amino acid uptake using the amino acid analog alpha-aminoisobutyric acid (AIB). This increased sensitivity resulted both from the use of serum free medium and the addition of dexamethasone to the serum free media. Pretreatment of the cells for 1, 2, or 3 days before assay showed that the maximum increase in sensitivity was obtained after a two day pretreatment. Pretreatment of the cells also increased their sensitivity to insulin and bovine insulin-like growth factor II stimulation of AIB uptake similar to that seen for IGF I. No consistent effect of the pretreatment was observed on either the basal level of AIB uptake or the maximal hormonal stimulation of AIB uptake. Nor was any change noted in the shape of the dose response curves. Addition of IGF I to the pretreatment medium greatly reduced the sensitivity of pretreated cells. [125I]IGF I binding studies done on suspended fibroblasts indicated that there was up to a two fold increase in the number of receptors with no increase in their affinity for IGF I. Thus, pretreatment of fibroblasts with dexamethasone and serum free medium greatly enhances their sensitivity to IGF I stimulation of AIB uptake and makes this an excellent in vitro bioassay system for IGF I.
journal_name
Endocr Resjournal_title
Endocrine researchauthors
Heath-Monnig E,Daughaday WHdoi
10.3109/07435808909042743subject
Has Abstractpub_date
1989-01-01 00:00:00pages
303-22issue
3eissn
0743-5800issn
1532-4206journal_volume
15pub_type
杂志文章abstract::Standard replacement for adrenal insufficiency (AI) consists of glucocorticoids and mineralocorticoids while DHEA deficiency is routinely ignored. Thus, AI represents the ideal pathophysiological model of isolated DHEA deficiency. We investigated the effects of DHEA replacement in 24 women with primary and secondary A...
journal_title:Endocrine research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.3109/07435800009048561
更新日期:2000-11-01 00:00:00
abstract::The effect of crowding on the pituitary-gonadal axis was studied in adult male rats. Crowding reduced body weight gain, did not alter relative adrenal weight and increased relative testis weight. Neither basal levels of corticosterone nor its response to acute stress were altered by crowding. Likewise, LH secretion wa...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808609035431
更新日期:1986-01-01 00:00:00
abstract::The sexual dimorphism of life span and caloric restriction effects in numerous species suggest that estradiol (E2) is protective against oxidative damage. The only direct test of E2's protective effect in mice against in vivo oxidative stress to date may have been confounded by E2's direct chemical action as an antiox...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435800902913600
更新日期:2009-01-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to investigate the effect of pioglitazone on serum uric acid (UA) levels and several diabetic parameters in drug-naïve subjects with type 2 diabetes mellitus (T2DM). PATIENTS AND METHODS:The patients (n = 68) received 7.5-30 mg/day pioglitazone monotherapy. They were divided into t...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2012.745128
更新日期:2013-08-01 00:00:00
abstract::It was the aim of this study to test for a possible effect of the new non ergot dopamine agonist CV 205-502 on plasma growth hormone (GH) concentrations in acromegaly. 10 acromegalic patients received a single oral dose of 150 micrograms CV 205-502 after an overnight fast. As a control group 7 acromegalic patients und...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809409035856
更新日期:1994-02-01 00:00:00
abstract::We established cell lines from adrenal tumors of transgenic mice harboring the large T-antigen of simian virus 40 under the control of the adrenocortical specific promoter of the scavenger aldose reductase-like akr1b7 gene. Mass spectrometry analyses of serum-supplemented or serum-free culture media showed that ATC1 l...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-200044168
更新日期:2004-11-01 00:00:00
abstract::Two P450c11 genes, sharing 99% homology, were cloned from Cape baboon adrenal tissue using RT-PCR. The cDNAs showed 96% and 94.6% sequence identity to human P450c11 and aldosterone synthase, respectively. One of the gene sequences contained a termination codon in exon one. The cloned cDNAs were expressed in COS 1 cell...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800009048632
更新日期:2000-11-01 00:00:00
abstract::The purpose of this study was to examine the effects of exercise on anterior pituitary hormones (adrenocorticotropic hormone (ACTH), leutinizing hormone (LH) and growth hormone) as well as on cortisol at low altitude (350 meters below the sea level) and to compare these effects with those at a moderate level altitude ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809609030513
更新日期:1996-08-01 00:00:00
abstract::In addition to regulating renal sodium resorption and, thus, intravascular volume, aldosterone may have direct effects on the cardiovascular system. We previously identified a polymorphism (-344C/T) in the promoter of the aldosterone synthase (CYP11B2) gene that affects binding of the SF-1 transcription factor and thu...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809809032690
更新日期:1998-08-01 00:00:00
abstract::Y1 cells derived from mouse tumor zona fasciculata cells (ZF) were used to assess the importance of extracellular (EC) calcium availability via voltage-dependent calcium channels (VDCC) on steroidogenesis. The steroidogenic response to ACTH was investigated in the presence of blockers known to affect both calcium and ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809609043745
更新日期:1996-11-01 00:00:00
abstract::Steroid 21-hydroxylase deficiency is the most frequent cause of congenital adrenal hyperplasia, an inherited inability to synthesize cortisol. Mutations causing this disorder have been characterized by hybridization analysis of patient DNA samples using cDNA and oligonucleotide probes, and by cloning and sequencing of...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435808909039099
更新日期:1989-01-01 00:00:00
abstract:OBJECTIVES:The relationship between thyroid status and renal function has been well characterized. However, renal function is affected by various factors, including sex and dysglycemia. Because these studies have yet to be undertaken in a normoglycemic population, this study sought to explore the association between th...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2011.640374
更新日期:2012-01-01 00:00:00
abstract::The Ca2(+)-ATPase of plasma membranes from a variety of tissues is subject to stimulation in vitro, and apparently in vivo, by physiological concentrations of iodothyronines regarded as biologically active in other bioassay systems. This calmodulin-dependent action of thyroid hormone is nongenomic, that is, directly o...
journal_title:Endocrine research
pub_type: 杂志文章,评审
doi:10.3109/07435808909036356
更新日期:1989-01-01 00:00:00
abstract:INTRODUCTION:The scavenger receptor class B type I (SR-BI) is a key component in the reverse cholesterol transportation. The aim of this study was to assess the association between exon1 (G → A) polymorphism of SR-BI gene and lipid profiles among the Tehran Lipid and Glucose Study (TLGS) population. MATERIALS AND METH...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2013.808207
更新日期:2014-01-01 00:00:00
abstract::In the guinea pig adrenal the cells of the outer zone secrete high levels of steroid and respond to ACTH with increased synthesis of cholesterol and steroid. The outer zone consists of two cell types: zona fasciculata (ZF) and zona glomerulosa (ZG). To determine the relative contribution of ZF and ZG to the outer zone...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809109027197
更新日期:1991-01-01 00:00:00
abstract::The question of whether ethanol's (ETOH's) known suppressive effect on serum luteinizing hormone (LH) could be mediated directly at the anterior pituitary level was addressed by examining the effects of ETOH in vitro on release of LH from cultured male rat pituitary cells. The impact of added ethanol concentrations ra...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435808909042748
更新日期:1989-01-01 00:00:00
abstract::Basal serum concentrations of prolactin (PRL) and the elevation of serum PRL concentrations induced by the serotonergic agents, quipazine and 5-hydroxytryptophan (5-HTP) were suppressed in rats treated acutely with dexamethasone. Acute dexamethasone treatment did not alter the stimulation of PRL release produced by th...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435808709023671
更新日期:1987-01-01 00:00:00
abstract:BACKGROUND:Ultrasonography is considered useful to distinguish between solid and cystic thyroid nodules and to stratify a nodule's risk of cancer as low, medium, or high. Ultrasound (US) elastography has been applied to study the hardness/elasticity of nodules to differentiate malignant from benign lesions. Elastograph...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2011.633952
更新日期:2012-01-01 00:00:00
abstract::Adrenal computed tomography with determination of Hounsfield units has proved to be sensitive and specific in the differential diagnosis of benign vs. malign adrenal lesions. On the other hand, computed tomography may fail in patients with small adrenal masses of less than 1.0 cm. However, especially in patients with ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120017075
更新日期:2002-11-01 00:00:00
abstract::It is now well documented that obesity is associated with a chronic low-grade inflammatory state. Levels of high-sensitivity C-reactive protein, a marker of systemic inflammation and a mediator of atherothrombotic disease, have been shown to correlate with cardiovascular disease risk. Our objective was to evaluate the...
journal_title:Endocrine research
pub_type: 临床试验,杂志文章
doi:10.1081/erc-200033541
更新日期:2004-08-01 00:00:00
abstract::Mouse Y1 adrenocortical tumor cells harbor amplified and overexpressed c-Ki-ras gene, displaying relatively high constitutive levels of Ras x GTP. Here we report that Y1 cells also constitutively display high levels of phosphorylated AKT/PKB, that are dependent on Ras x GTP and PI3K. ACTH rapidly causes dephosphorylat...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800009048616
更新日期:2000-11-01 00:00:00
abstract:INTRODUCTION:Impairments in neuroendocrine regulation of food intake and postprandial satiety are leading causes to obesity. Ghrelin peptide is a GI hormone known to increase food intake partly through induction of growth hormone. The regulation of ghrelin production is still unknown. OBJECTIVE:The aim of the current ...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2013.799484
更新日期:2014-01-01 00:00:00
abstract::The zonal distribution of aldosterone synthase cytochrome P450 (P450aldo) in the adrenal cortex of male hamsters was investigated by immunofluorescence and electron microscopy, using an anti-P450aldo peptide antibody. On cryostat sections the immunolocalization of P450aldo was confined to the zona glomerulosa cells. O...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809509030443
更新日期:1995-02-01 00:00:00
abstract:PURPOSE:The purpose of this study was to evaluate the expression of the glucose transporters GLUT1 and GLUT3 in papillary thyroid carcinomas (PTCs) and to elucidate their relationship with the BRAF V600E mutation and F-18 FDG uptake. MATERIALS AND METHODS:We retrospectively analyzed data of 52 PTC patients (41 women a...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2015.1066803
更新日期:2016-01-01 00:00:00
abstract::Comparison of binding of 125I-porcine FSH to porcine granulosa cells from small and large ovarian follicles indicated that binding is dependent on both time and temperature. At 37 C, binding of ligand is more than four times higher in cells from small follicles than in cells from large follicles within 30 minutes of t...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1080/07435809409035857
更新日期:1994-02-01 00:00:00
abstract::CYP17 is not expressed in adult mouse adrenal glands but is expressed in a subset of fetal adrenocortical cells, indicating the potential to produce both corticosterone and cortisol during murine embryogenesis. CYP11A is expressed in the fetal adrenal but also in developing hindgut, which will form the colon, and in c...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809509030425
更新日期:1995-02-01 00:00:00
abstract:OBJECTIVE:Antithyroid drugs such as propylthiouracil (PTU) and methimazole (MMI) are common medications in Chinese patients with hyperthyroidism and PTU-induced antineutrophil cytoplasmic antibody (ANCA) positive vasculitis has been reported. The current cross-sectional study aimed to investigate the prevalence and the...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.1081/erc-120037729
更新日期:2004-05-01 00:00:00
abstract::The stimulation of the zona glomerulosa phenotype by a low sodium diet is characterised by increased expression of aldosterone synthase accompanied by increases in (pro)renin, bFGF, c-fos and c-jun gene transcription. In contrast ACTH diminishes the specific glomerulosa phenotype. The EGF related transmembrane protein...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435809809032720
更新日期:1998-08-01 00:00:00
abstract:UNLABELLED:Absract Purpose: Mutations in the TPO gene have been reported to cause congenital hypothyroidism (CH), and our aim in this study was to determine the genetic basis of congenital hypothyroidism in two affected children coming from a consanguineous family. METHODS:Since CH is usually inherited in autosomal re...
journal_title:Endocrine research
pub_type: 杂志文章
doi:10.3109/07435800.2014.967354
更新日期:2015-01-01 00:00:00
abstract::There are striking similarities between Cushing's syndrome and the 'metabolic syndrome X' since both are characterised by hypertension, insulin resistance, glucose intolerance, hyperlipidaemia, and central obesity. The possibility that cortisol contributes to the associations between multiple risk factors for cardiova...
journal_title:Endocrine research
pub_type: 杂志文章,评审
doi:10.1080/07435809609043765
更新日期:1996-11-01 00:00:00