当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Multiple myeloma cells adapted to long-exposure of hypoxia exhibit stem cell characters with TGF-β/Smad pathway activation.

Multiple myeloma cells adapted to long-exposure of hypoxia exhibit stem cell characters with TGF-β/Smad pathway activation.

Abstract：

:The emergence of new molecular targeting agents has improved the prognosis of patients with multiple myeloma (MM). However, MM remains incurable because MM stem cells are likely resistant to these agents. Thus, it is important to further investigate the biology of MM stem cells, which reside in the hypoxic bone marrow niche. In this study, we established and investigated the characteristics of hypoxia-adapted MM (HA-MM) cells, which could proliferate for more than six months under hypoxic conditions (1% O2). The G0 fraction of HA-MM cells was larger than that of parental MM cells under normoxic conditions (20% O2). HA-MM cells possess enhanced tumorigenicity in primary and secondary transplantation studies. HA-MM cells also exhibited increased mRNA levels of stem cell markers and an enhanced self-renewal ability, and thus demonstrated characteristics of MM stem cells. These cells overexpressed phosphorylated Smad2, and treatment with a transforming growth factor (TGF)-β/Smad signaling inhibitor decreased their clonogenicity in a replating assay. In conclusion, MM cells adapted to long-exposure of hypoxia exhibit stem cell characters with TGF-β/Smad pathway activation.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Nakagawa Y,Ashihara E,Yao H,Yokota A,Toda Y,Miura Y,Nakata S,Hirai H,Maekawa T

doi

10.1016/j.bbrc.2018.01.034

subject

Has Abstract

pub_date

2018-02-05 00:00:00

pages

490-496

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(18)30040-8

journal_volume

496

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • UDP-glucose pyrophosphorylase Ugp1 is involved in oxidative stress response and long-term survival during stationary phase in Saccharomyces cerevisiae.

    abstract：:Ugp1, UDP-glucose pyrophosphorylase, plays an important role in carbohydrate metabolism because it provides UDP-glucose that is a pivotal metabolite in several metabolic pathways in Saccharomyces cerevisiae. In this study, we show that a considerable reduction of glycogen and trehalose content in ugp1 knockdown cells ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.10.090

    authors： Yi DG,Huh WK

    更新日期：2015-11-27 00:00:00

  • A distinct form of tau is selectively incorporated into Alzheimer's paired helical filaments.

    abstract：:Tau, a microtubule-associated phosphoprotein, was identified as a definite component of paired helical filaments which progressively accumulate in Alzheimer's disease brain. To learn more about tau in the aged brain, we have isolated and sequenced a cDNA clone encoding tau from a cDNA library of an aged human brain. T...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(89)92240-7

    authors： Mori H,Hamada Y,Kawaguchi M,Honda T,Kondo J,Ihara Y

    更新日期：1989-03-31 00:00:00

  • Expression and regulation of calcium-independent protein kinase C in NG 108-15 cell differentiation.

    abstract：:Protein, mRNA and activity levels of the calcium-independent protein kinase C (nPKC) isoenzymes were examined in NG108-15 cells. Western blot analyses reveal that proliferating NG 108-15 cells express the delta, epsilon, and eta, but not the theta species. The atypical species PKC zeta was also detected. Differentiati...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.2344

    authors： Battaini F,Pascale A,Lucchi L,Racchi M,Bergamaschi S,Parenti M,Wetsel WC,Govoni S,Trabucchi M

    更新日期：1994-09-30 00:00:00

  • Upstream stimulatory factor 1 (USF1) suppresses induction of CYP1A1 mRNA by 3-methylcholanthrene (MC) in HepG2 cells.

    abstract：:In this study, an endogenous factor(s) involved in the suppression of the induction of CYP1A1 was studied. Analyzing the sequences, we found that the sequence of xenobiotic responsive element (XRE) in the upstream region of the human CYP1A1 gene was overlapped with that of the upstream stimulatory factor 1 (USF1)-bind...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.7651

    authors： Takahashi Y,Nakayama K,Itoh S,Kamataki T

    更新日期：1997-11-17 00:00:00

  • Knockout of neutrophil elastase protects against western diet induced nonalcoholic steatohepatitis in mice by regulating hepatic ceramides metabolism.

    abstract：:Previous studies reported increased expression and activity of neutrophil elastase (NE) in NASH. However, the role of NE in nonalcoholic steatohepatitis (NASH) remained unclear. Wild type (WT) and NE knockout (NE KO) mice were fed with western diet (WD) for 24 weeks to establish NASH model. The severity of liver injur...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.08.111

    authors： Chen J,Liang B,Bian D,Luo Y,Yang J,Li Z,Zhuang Z,Zang S,Shi J

    更新日期：2019-10-22 00:00:00

  • Dynamic changes in expression of heme oxygenases in mouse heart and liver during hypoxia.

    abstract：:Heme oxygenase cleaves heme to form biliverdin, carbon monoxide (CO), and iron, and consists of two structurally related isozymes, HO-1 and HO-2. HO-2 is also known as a potential oxygen sensor. Here we show that the relative CO content in arterial blood, which reflects the total amount of endogenous heme degradation,...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.08.100

    authors： Han F,Takeda K,Yokoyama S,Ueda H,Shinozawa Y,Furuyama K,Shibahara S

    更新日期：2005-12-09 00:00:00

  • Heat shock induces phosphorylation of histone H2AX in mammalian cells.

    abstract：:Heat shock induces a variety of biological events including gene activation, cell cycle arrest, and apoptosis. Heat shock has recently been shown to be potentially useful when combined with radiation in cancer therapy, probably because, in mammalian cells, heat inhibits the repair of double-strand breaks (DSBs) induce...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.01.073

    authors： Kaneko H,Igarashi K,Kataoka K,Miura M

    更新日期：2005-03-25 00:00:00

  • Hyaluronan binding protein 1 (HABP1)/C1QBP/p32 is an endogenous substrate for MAP kinase and is translocated to the nucleus upon mitogenic stimulation.

    abstract：:The role of hyaluronan binding protein 1 (HABP1) in cell signaling was investigated and in vitro kinase assay demonstrated that it is a substrate for MAP kinase. Phosphorylation of endogenous HABP1 was also observed following treatment of J774 cells with PMA. HABP1 was coimmunoprecipitated with activated ERK, confirmi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2002.6491

    authors： Majumdar M,Meenakshi J,Goswami SK,Datta K

    更新日期：2002-03-08 00:00:00

  • Inhibition of ADP-ribosyltransferase activity of cholera toxin by MDL 12330A and chlorpromazine.

    abstract：:ADP-ribosylation by cholera toxin of the guanine nucleotide binding regulatory protein (Gs) of rat liver membrane adenylate cyclase was inhibited by 0.1-1 mM MDL 12330A or 0.1-1 mM chlorpromazine. Basal as well as cholera toxin activated adenylate cyclase activity in liver membranes was also inhibited by the two drugs...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(84)91312-3

    authors： Bitonti AJ

    更新日期：1984-04-30 00:00:00

  • The Tax oncogene enhances ELL incorporation into p300 and P-TEFb containing protein complexes to activate transcription.

    abstract：:The eleven-nineteen lysine-rich leukemia protein (ELL) is a key regulator of RNA polymerase II mediated transcription. ELL facilitates RNA polymerase II transcription pause site entry and release by dynamically interacting with p300 and the positive transcription elongation factor b (P-TEFb). In this study, we investi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.07.072

    authors： Fufa TD,Byun JS,Wakano C,Fernandez AG,Pise-Masison CA,Gardner K

    更新日期：2015-09-11 00:00:00

  • Synergistic regulation of the mouse orphan nuclear receptor SHP gene promoter by CLOCK-BMAL1 and LRH-1.

    abstract：:Small heterodimer partner (SHP; NR0B2) is an orphan nuclear receptor and acts as a repressor for wide variety of nuclear hormone receptors. We demonstrated here that mouse SHP mRNA showed a circadian expression pattern in the liver. Transient transfection of the mSHP promoter demonstrated that CLOCK-BMAL1, core circad...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2006.12.131

    authors： Oiwa A,Kakizawa T,Miyamoto T,Yamashita K,Jiang W,Takeda T,Suzuki S,Hashizume K

    更新日期：2007-02-23 00:00:00

  • Interaction of the two soluble metal-binding domains of yeast Ccc2 with copper(I)-Atx1.

    abstract：:Yeast Ccc2 is a P-type ATPase responsible for transport of copper(I) from the cytosol to the trans-Golgi network. It possesses a soluble cytosolic N-terminal region containing two copper(I)-binding domains. Homologous eukaryotic copper-transporting ATPases have from one to six domains. We have expressed a fragment enc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2007.10.045

    authors： Banci L,Bertini I,Chasapis CT,Rosato A,Tenori L

    更新日期：2007-12-21 00:00:00

  • Independent inhibition of DNA synthesis by protein kinase C, cyclic AMP and interferon alpha/beta in rabbit aortic smooth muscle cells.

    abstract：:In quiescent cultures of rabbit aortic smooth muscle cells, whole blood serum-induced DNA synthesis was inhibited markedly by protein kinase C-activating 12-O-tetradecanoyl-phorbol-13-acetate (TPA) and phorbol-12, 13-dibutyrate (PDBu), cyclic AMP-derivatives, such as dibutyryl cyclic AMP (Bt2cAMP) and 8-bromo-cyclic A...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80052-4

    authors： Fukumoto Y,Kawahara Y,Kariya K,Araki S,Fukuzaki H,Takai Y

    更新日期：1988-11-30 00:00:00

  • Preliminary assessment of the C13-side chain 2'-hydroxylase involved in taxol biosynthesis.

    abstract：:The biosynthesis of the anticancer drug Taxol in yew (Taxus) species is thought to involve the preliminary formation of the advanced taxane diterpenoid intermediate baccatin III upon which the functionally important N-benzoyl phenylisoserinoyl side chain is subsequently assembled at the C13-O-position. In vivo feeding...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.08.119

    authors： Long RM,Croteau R

    更新日期：2005-12-09 00:00:00

  • Molecular analysis of an acatalasemic mouse mutant.

    abstract：:The Csb acatalasemia mouse mutant differentially expresses reduced levels of catalase activity in a tissue specific manner. In order to pinpoint the molecular lesion that imparts the acatalasemia phenotype in Csb mice we have utilized the polymerase chain reaction technique to isolate catalase cDNA clones from control...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80891-5

    authors： Shaffer JB,Preston KE

    更新日期：1990-12-31 00:00:00

  • Drosophila melanogaster deoxyribonucleoside kinase activates gemcitabine.

    abstract：:Drosophila melanogaster multisubstrate deoxyribonucleoside kinase (Dm-dNK) can additionally sensitize human cancer cell lines towards the anti-cancer drug gemcitabine. We show that this property is based on the Dm-dNK ability to efficiently phosphorylate gemcitabine. The 2.2A resolution structure of Dm-dNK in complex ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.03.041

    authors： Knecht W,Mikkelsen NE,Clausen AR,Willer M,Eklund H,Gojković Z,Piskur J

    更新日期：2009-05-01 00:00:00

  • Construction of nerve guide conduits from cellulose/soy protein composite membranes combined with Schwann cells and pyrroloquinoline quinone for the repair of peripheral nerve defect.

    abstract：:Regeneration and functional reconstruction of peripheral nerve defects remained a significant clinical challenge. Nerve guide conduits, with seed cells or neurotrophic factors (NTFs), had been widely used to improve the repair and regeneration of injured peripheral nerve. Pyrroloquinoline quinone (PQQ) was an antioxid...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.12.121

    authors： Luo L,Gan L,Liu Y,Tian W,Tong Z,Wang X,Huselstein C,Chen Y

    更新日期：2015-02-20 00:00:00

  • CVD-associated non-coding RNA, ANRIL, modulates expression of atherogenic pathways in VSMC.

    abstract：:ANRIL is a newly discovered non-coding RNA lying on the strongest genetic susceptibility locus for cardiovascular disease (CVD) in the chromosome 9p21 region. Genome-wide association studies have been linking polymorphisms in this locus with CVD and several other major diseases such as diabetes and cancer. The role of...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.02.050

    authors： Congrains A,Kamide K,Katsuya T,Yasuda O,Oguro R,Yamamoto K,Ohishi M,Rakugi H

    更新日期：2012-03-23 00:00:00

  • Evidence for the alpha-helicity of class II MHC molecular binding sites for the superantigen, staphylococcal enterotoxin A.

    abstract：:Circular dichroism (CD) spectra of class II MHC peptides revealed the alpha-helical conformation of superantigen-binding peptides I-A beta b(60-90), I-A beta b(65-85), and I-A alpha b(51-80), but not the nonbinding peptide I-A beta b(80-100). These CD spectra provide biophysical evidence for the alpha-helicity of clas...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)91833-c

    authors： Russell JK,Jarpe MA,Johnson HM

    更新日期：1992-02-14 00:00:00

  • Smad3 contributes to positioning of proliferating cells in colonic crypts by inducing EphB receptor protein expression.

    abstract：:Deficiency of Smad3, an intracellular mediator of TGF-β, was shown to significantly accelerate re-epithelialization of the colonic mucosa. This study was performed to investigate the molecular mechanisms by which Smad3 controls colonic epithelial cell proliferation and crypt formation. Smad3(ex8/ex8) C57BL/6 mice were...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.01.045

    authors： Furukawa K,Sato T,Katsuno T,Nakagawa T,Noguchi Y,Tokumasa A,Yokote K,Yokosuka O,Saito Y

    更新日期：2011-02-25 00:00:00

  • Calcium-activated, calmodulin-dependent protein kinase activity in bovine thyroid cytosol.

    abstract：:Bovine thyroid 100,000 X g supernatant contained calcium-activated, calmodulin-dependent protein kinase (PK-CaM) activity. The PK-CaM was partially purified using ion-exchange chromatography and characterized. PK-CaM, using casein as exogenous substrate, was not stimulated by Ca2+(0-500 microM) or calmodulin (1-10 mic...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)91066-1

    authors： Friedman Y,Henricks L,Poleck T,Levasseur S,Burke G

    更新日期：1986-10-15 00:00:00

  • Purification and partial sequencing of human placental alkaline phosphatase.

    abstract：:Two forms of human placental alkaline phosphatase have been purified to homogeneity utilizing high performance liquid chromatography. Both have the same amino acid composition but they differ in their carbohydrate substituents. Sequence data indicate that the two forms are identical for the first forty two residues fr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(83)80252-6

    authors： Ezra E,Blacher R,Udenfriend S

    更新日期：1983-11-15 00:00:00

  • Protein arginine methyltransferase 6 specifically methylates the nonhistone chromatin protein HMGA1a.

    abstract：:The HMGA family proteins HMGA1a and HMGA1b are nuclear nonhistone species implicated in a wide range of cellular processes including inducible gene transcription, modulation of chromosome structure through nucleosome and chromosome remodeling, and neoplastic transformation. HMGA proteins are highly modified, and chang...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.08.179

    authors： Miranda TB,Webb KJ,Edberg DD,Reeves R,Clarke S

    更新日期：2005-10-28 00:00:00

  • Identification of early gene expression changes in primary cultured neurons treated with topoisomerase I poisons.

    abstract：:Topoisomerase 1 (TOP1) poisons like camptothecin (CPT) are currently used in cancer chemotherapy but these compounds can have damaging, off-target effects on neurons leading to cognitive, sensory and motor deficits. To understand the molecular basis for the enhanced sensitivity of neurons to CPT, we examined the effec...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2016.09.068

    authors： Rossi SL,Lumpkin CJ,Harris AW,Holbrook J,Gentillon C,McCahan SM,Wang W,Butchbach MER

    更新日期：2016-10-14 00:00:00

  • Substance P regulates the function of rabbit cultured osteoclast; increase of intracellular free calcium concentration and enhancement of bone resorption.

    abstract：:The effects of substance P on cultured rabbit osteoclasts were investigated. The intracellular Ca(2+) concentration ([Ca(2+)](i)) which was monitored by the microfluorometric technique using fura-2, in osteoclasts elevated by the addition of substance P (0.3-5 microM). The EC(50) value of substance P was about 200 nM....

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1220

    authors： Mori T,Ogata T,Okumura H,Shibata T,Nakamura Y,Kataoka K

    更新日期：1999-08-27 00:00:00

  • Down regulation of CD4 expression in cultured microglia by immunosuppressants and lipopolysaccharide.

    abstract：:Microglia, brain macrophages, are thought to be the primary target of HIV-1 infection in the brain, because they exclusively express the CD4 antigen which is effectively used for viral entry. The expression of CD4 mRNA in cultured microglia could be detected by the reverse-PCR method. Using this and immunohistochemica...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)92284-5

    authors： Sawada M,Suzumura A,Marunouchi T

    更新日期：1992-12-15 00:00:00

  • Variable allelic expression of imprinted genes in human pluripotent stem cells during differentiation into specialized cell types in vitro.

    abstract：:Genomic imprinting is an epigenetic phenomenon by which a subset of genes is asymmetrically expressed in a parent-of-origin manner. However, little is known regarding the epigenetic behaviors of imprinted genes during human development. Here, we show dynamic epigenetic changes in imprinted genes in hESCs during in vit...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.02.141

    authors： Park SW,Kim J,Park JL,Ko JY,Im I,Do HS,Kim H,Tran NT,Lee SH,Kim YS,Cho YS,Lee DR,Han YM

    更新日期：2014-04-04 00:00:00

  • Stimulation of aromatase activity in immature porcine Leydig cells by fibroblast growth factor (FGF).

    abstract：:The effects of fibroblast growth factor (FGF) on testicular aromatase activity has been studied using primary cultures of porcine Leydig cells. After culture for 3 days in the absence or presence of FGF, the ability of the cells to produce estrogen was examined in a 4h-test period in which either (a) hCG (10(-9) M) or...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(88)90574-8

    authors： Raeside JI,Berthelon MC,Sanchez P,Saez JM

    更新日期：1988-02-29 00:00:00

  • Acetylsalicylic acid antagonizes the bleeding-induced changes in hemoglobin proportions in normal adult rats.

    abstract：:Normal adult Sprague-Dawley rats were made anemic by repeated phlebotomy. Ion-exchange chromatography of anemic blood showed newborn like hemoglobin proportions, involving the same six hemoglobin components as is found when newborn and adult blood are compared. However, acetylsalicylic acid intake during anemia failed...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(86)91176-9

    authors： Datta MC,Josephs J,Tolbert ME,Anderson J,Dowla H

    更新日期：1986-05-29 00:00:00

  • Ligand-induced functions of the epidermal growth factor receptor require the positively charged region asymmetrically distributed across plasma membrane.

    abstract：:Many plasma membrane proteins, including the epidermal growth factor (EGF) receptor, possess basic regions on the cytoplasmic surface of the membrane. To examine the function of these positively charged regions, we constructed mutated EGF receptor genes lacking this region by substitution of the basic amino acid resid...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80024-5

    authors： Yamane K,Toyoshima C,Nishimura S

    更新日期：1992-05-15 00:00:00