Abstract:
:Disuse osteopenia and bone loss have been extensively reported in long duration space mission and long term bed rest. The pathology of the bone loss is similar to osteoporosis but highly confined to weight bearing bones. The current anabolic and/or anti-resorptive drugs have systemic effects and are costly over extended time, with concerns of long term fracture risk. This study use Low Intensity Pulsed Ultrasound (LIPUS) as a non-invasive acoustic force and anabolic stimulus to countermeasure disuse induced bone loss. Four-month old C57BL/6 mice were randomized into five groups, 1) age-matched (AM), 2) non-suspended sham (NS), 3) non-suspended-LIPUS (NU), 4) suspended sham (SS), and 5) suspended-LIPUS (SU) groups. After four weeks of suspension, μCT analyses showed significant decreases in trabecular bone volume fraction (BV/TV) (-36%, p<0.005), bone tissue mineral density (TMD) (-3%, p<0.05), trabecular thickness (Tb.Th) (-12.5%, p<0.005), and increase in bone surface/bone volume (+BS/BV) (+16%, p<0.005), relative to age-matched (AM). The application of LIPUS for 20 min/day for 5 days/week, significantly increased TMD (+3%, p<0.05), Tb.Th (+6%, p<0.05), and decreased BS/BV (-10%, p<0.005), relative to suspension alone (SS) mice. Histomorphometry analyses showed a breakdown of bone microstructure under disuse conditions consist with μCT results. In comparison to SS mice, LIPUS treated bone showed increased structural integrity with increased bone formation rates at metaphysical endosteal and trabecular surfaces (+0.104±0.07 vs 0.031±0.30 μm(3)/μm(2)/day) relative to SS. Four-point bending mechanical tests of disused SS femurs showed reduced elastic modulus (-53%, p<0.05), yield (-33%, p<0.05) and ultimate strength (-45%, p<0.05) at the femoral diaphysis relative to AM bone. LIPUS stimulation mitigated the adverse effects of disuse on bone elastic modulus (+42%, p<0.05), yield strength (+29%, p<0.05), and ultimate strength (+39%, p<0.05) relative to SS femurs. LIPUS provides the essential mechanical stimulus to retain bone morphological and mechanical integrity in disuse conditions. This study demonstrates LIPUS potential as regional therapeutic agent to countermeasure disuse induced bone loss while maintaining bone's integrity.
journal_name
Bonejournal_title
Boneauthors
Uddin SM,Qin YXdoi
10.1016/j.bone.2015.01.020subject
Has Abstractpub_date
2015-06-01 00:00:00pages
8-17eissn
8756-3282issn
1873-2763pii
S8756-3282(15)00034-4journal_volume
75pub_type
杂志文章相关文献
BONE文献大全abstract::The SIBLINGs are a subfamily of the secreted calcium-binding phosphoproteins and comprise five small integrin-binding ligand N-linked glycoproteins [dentin matrix protein-1 (DMP1), secreted phosphoprotein-1 (SPP1) also called osteopontin (OPN), integrin-binding sialoprotein (IBSP) also called bone sialoprotein (BSP), ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2019.115190
更新日期:2020-03-01 00:00:00
abstract::Bone modeling, the process that continually adjusts bone strength in response to prevalent muscle-loading forces throughout an individual's lifespan, may play an important role in bone fragility with age. Femoral stress, an index of bone modeling response, can be estimated using measurements of DXA derived bone geomet...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2016.06.012
更新日期:2016-09-01 00:00:00
abstract::The underlying causes of maxillary bone loss during lactation remain poorly understood. We evaluated the impact of lactation on physiological and mechanically-induced alveolar bone remodeling. Nulliparous non-lactating (N-LAC) and 21-day lactating (LAC) mice underwent mechanically-induced bone remodeling by orthodonti...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2018.01.032
更新日期:2018-05-01 00:00:00
abstract::Nanoindentation has been widely used to study bone tissue mechanical properties. The common method and equations for analyzing nanoindentation, developed by Oliver and Pharr, are based on the assumption that the material is linearly elastic. In the present study, we adjusted the constraint of linearly elastic behavior...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2008.05.023
更新日期:2008-10-01 00:00:00
abstract::The effects of pregnancy and lactation on bone histomorphometry have been studied extensively in rats and dogs. However, these models differ greatly in reproductive physiology compared with women. The purpose of this study was to evaluate histomorphometric changes in iliac crest bone biopsies taken from cynomolgus mon...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(98)00032-5
更新日期:1998-05-01 00:00:00
abstract:BACKGROUND:Episodic flare-ups of fibrodysplasia ossificans progressiva (FOP) are characterized clinically by severe, often posttraumatic, connective tissue swelling and intramuscular edema, followed histologically by an intense and highly angiogenic fibroproliferative reaction. This early inflammatory and angiogenic fi...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.08.023
更新日期:2018-04-01 00:00:00
abstract::The responses of bone cells to skeletal loading are clearly an important factor in bone biology, but much remains to be learned about the role of these responses in skeletal development, maintenance, and tissue repair. Bone morphogenetic proteins (BMPs) are key regulators of bone formation. We examined the effect of B...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(02)00865-7
更新日期:2002-11-01 00:00:00
abstract::Osteopontin (OPN) gene expression and alkaline phosphatase activity were evaluated in the epiphyseal growth plates of normal chickens and in diet-induced tibial dyschdroplasia (TD)-afflicted chickens. In the normal growth plate, OPN gene was expressed by a) cells of the subperichondrial zone surrounding the articular ...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/8756-3282(94)00048-5
更新日期:1995-04-01 00:00:00
abstract::Aging is associated with gradual bone loss in men and premenopausal women, with an accelerated rate of loss after menopause in women. Although many studies have investigated bone loss due to surgically induced estrogen depletion, little is known regarding normal age-related changes in bone mass in animal models. We us...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(00)00452-x
更新日期:2001-03-01 00:00:00
abstract::Mandibular condylar cartilage can be distinguished from articular and growth cartilages of long bones based on several differences in morphology, physiology, and function between these structures. However, there is almost no information available on the types of genes that contribute to these differences. In this stud...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2007.09.048
更新日期:2008-06-01 00:00:00
abstract:INTRODUCTION:The elderly population is growing fast worldwide, and therefore, investigation of health outcomes peculiar to these individuals is a public health priority nowadays. The decade between 2000 and 2010 is denominated as the Bone and Joint Decade, and researchers are encouraged to quantity the burden of muscul...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2005.04.002
更新日期:2005-08-01 00:00:00
abstract::Bone strength in adulthood is known to be affected by health at birth and early childhood. Habitual bone loading is a primary determinant of bone strength in later childhood and adulthood. However, the effects of physical activity in early childhood (e.g. crawling, standing and walking) on bone strength are unknown. F...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2014.08.003
更新日期:2014-11-01 00:00:00
abstract::Osteocyte apoptosis precedes osteoclast resorption, and may act as a critical signal to trigger bone remodeling. While osteoclast precursors are known to travel via the circulation, the specific mechanisms by which they accumulate at remodeling sites are unclear. We hypothesized that osteocyte apoptosis mediates osteo...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.09.052
更新日期:2012-01-01 00:00:00
abstract::Hypophosphatasia (HPP) is caused by loss-of-function mutation(s) within the gene TNSALP that encodes the "tissue-nonspecific" isoenzyme of alkaline phosphatase (TNSALP). In HPP, inorganic pyrophosphate, an inhibitor of mineralization and substrate for TNSALP, accumulates extracellularly often leading to rickets or ost...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2015.02.022
更新日期:2015-06-01 00:00:00
abstract::We have previously shown that the therapeutic range of ultrasound heals osteoradionecrotic bone and induces bone formation in vitro. It is well established that nitric oxide (NO) and prostaglandins are crucial early mediators in mechanically induced bone formation. The therapeutic range of ultrasound may act in the sa...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(02)00789-5
更新日期:2002-07-01 00:00:00
abstract::The association between sports participation during adolescence and peri- and postmenopausal bone mineral density (BMD) was examined among 2025 women aged 48-58 years. Adolescent recreational and competitive sporting activities were registered with a self-administered questionnaire. Altogether, 881 (43.5%) women state...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(97)00148-8
更新日期:1997-10-01 00:00:00
abstract::Osteoblast-like cells express receptors for various hormones and neurotransmitters that induce widespread actions in the bone to which intercellular communication and its modulation may contribute. Therefore, we examined the effects of the osteotropic hormones vitamin D3 (vitD3) and 17beta-estradiol (17beta-E2) as wel...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(98)00142-2
更新日期:1998-12-01 00:00:00
abstract::Bone is a complex endocrine organ that facilitates structural support, protection to vital organs, sites for hematopoiesis, and calcium homeostasis. The bone marrow microenvironment is a heterogeneous niche consisting of multipotent musculoskeletal and hematopoietic progenitors and their derivative terminal cell types...
journal_title:Bone
pub_type: 杂志文章,评审
doi:10.1016/j.bone.2018.05.019
更新日期:2019-01-01 00:00:00
abstract::Clinical bone sonometers applied at the calcaneus measure broadband ultrasound attenuation and speed of sound. However, the relation of ultrasound measurements to bone strength is not well-characterized. Addressing this issue, we assessed the extent to which ultrasonic measurements convey in vitro mechanical propertie...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2017.06.021
更新日期:2017-10-01 00:00:00
abstract::Gap junctional intercellular communication (GJIC) may contribute to cellular differentiation. To examine this possibility in bone cells we examined markers of cellular differentiation, including alkaline phosphatase, osteocalcin, and osteopontin, in ROS17/2.8 cells (ROS), a rat osteoblastic cell line expressing phenot...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(99)00227-6
更新日期:1999-12-01 00:00:00
abstract::Matrix extracellular phosphoglycoprotein (MEPE) is an extracellular matrix protein that was first detected in tumor-induced osteomalacia (TIO). Investigations in mice revealed that MEPE is expressed in bone and teeth in a maturation-dependent manner, reaching its maximum during mineralization. However, from knockout e...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2004.03.033
更新日期:2004-08-01 00:00:00
abstract::Bone structure is the key to the understanding of fracture risk. The hypothesis tested in this prospective study is that dense mandibular trabeculation predicts low fracture risk, whereas sparse trabeculation is predictive of high fracture risk. Out of 731 women from the Prospective Population Study of Women in Gothen...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2011.06.036
更新日期:2011-10-01 00:00:00
abstract::To study the osteoinductive action of hyaluronic acid (HA), we examined the effects of applying an elastoviscous high-molecular HA preparation on bone wound healing after bone marrow ablation. The middiaphyses of cortical bones from rat femurs were perforated with a round bar, and excavated marrow cavities were filled...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/s8756-3282(94)00001-8
更新日期:1995-01-01 00:00:00
abstract::This study reports the effects of a recreational team handball exercise programme (randomised controlled trial, RCT) on bone health, postural balance and body composition in inactive postmenopausal women without previous experience of the sport. Sixty-seven postmenopausal women (68.3 ± 6.2 years, stature 156.9 ± 5.8 c...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2021.115847
更新日期:2021-01-13 00:00:00
abstract::Bone healing is a complex multi-step process, which depends on the position and size of the lesion, and on the mechanical stability of the wounded area. To address more specifically the mechanisms involved in cortical bone healing, we created drill-hole defects in the cortex of mouse femur, a lesion that triggers intr...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.09.007
更新日期:2010-02-01 00:00:00
abstract::Eldecalcitol (ED-71), a 2β-hydroxypropyloxy derivative of 1α,25(OH)(2)D(3), inhibits bone resorption more potently than does alfacalcidol while maintaining osteoblastic function in an estrogen-deficient, high-turnover osteoporosis rat model. Alendronate (ALN) has been reported to increase bone mass by suppressing bone...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2012.02.005
更新日期:2012-05-01 00:00:00
abstract::Fracture healing is impaired in osteoporotic bone. Low-magnitude high-frequency vibration (LMHFV) has recently been proven to be osteogenic in osteoporotic intact bone. Our previous study found that LMHFV significantly enhanced fracture healing in adult rats. This study was designed to explore whether LMHFV was able t...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2009.11.028
更新日期:2010-05-01 00:00:00
abstract:PURPOSE:Bone acquisition in childhood impacts adult bone mass, and can be influenced by childhood socioeconomic conditions. Socioeconomic status is also associated with body weight which affects the load that bone is exposed to in a fall. We hypothesized that socioeconomic advantage in childhood is associated with grea...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2013.06.021
更新日期:2013-10-01 00:00:00
abstract::The enlargement of osteocyte lacunae via osteocytic osteolysis was previously detected in situations of increased calcium demand (e.g., lactation, vitamin D deficiency). However, it is unclear whether similar processes occur also in the growing infantile skeleton and how this is linked to the mineral distribution with...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115324
更新日期:2020-06-01 00:00:00
abstract:PURPOSE:Diabetes and osteoporosis occur frequently in older adults and are both associated with increased fracture risk. Denosumab treatment reduced new vertebral, nonvertebral, and hip fractures over 3 years, with continued low fracture incidence for up to 10 years in postmenopausal women with osteoporosis. However, i...
journal_title:Bone
pub_type: 杂志文章
doi:10.1016/j.bone.2020.115268
更新日期:2020-05-01 00:00:00