Evidence of downregulation of matrix extracellular phosphoglycoprotein during terminal differentiation in human osteoblasts.


:Matrix extracellular phosphoglycoprotein (MEPE) is an extracellular matrix protein that was first detected in tumor-induced osteomalacia (TIO). Investigations in mice revealed that MEPE is expressed in bone and teeth in a maturation-dependent manner, reaching its maximum during mineralization. However, from knockout experiments, although it has become clear that MEPE might function as a mineralization inhibitor, the exact mechanism of action is still unclear. Even less is known about the regulation of MEPE in men. Therefore, we have studied the time- and maturation-dependent expression of MEPE in two human osteoblast culture systems, the osteosarcoma cell line HOS 58 and primary trabecular osteoblasts. Cells were cultured for up to 29 days, and the influence of beta-glycerophosphate (bGP), ascorbate, transforming growth factor beta (TGF-beta), BMP-2, and dexamethasone was studied. HOS 58 cells showed no significant effect on MEPE gene expression up to 5.0 mM, but a significant inhibition was revealed at 10 and 20 mM, when osteocalcin (OC) expression was maximal. Under the same conditions, primary human osteoblasts showed no effect on MEPE gene expression. However, when cultured in the presence of 5 mM beta-glycerophosphate, ascorbate, and dexamethasone for 29 days, which are similar conditions to those described by Owen in his differentiation model in rat osteoblasts, a progressive inhibition of MEPE gene expression to 20% of the maximum was observed. Increasing osteocalcin expression indicated advancing differentiation. In conclusion, in contrast to the results in mice, when MEPE was maximally expressed during mineralization, in the human system, this factor seems to be maximally active in the proliferation and early matrix maturation phase. It was, however, strongly suppressed, associated with the mineralization phase.






Siggelkow H,Schmidt E,Hennies B,Hüfner M




Has Abstract


2004-08-01 00:00:00
















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    authors: Adami G,Arioli G,Bianchi G,Brandi ML,Caffarelli C,Cianferotti L,Gatti D,Girasole G,Gonnelli S,Manfredini M,Muratore M,Quarta E,Quarta L

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  • Globular adiponectin reverses osteo-sarcopenia and altered body composition in ovariectomized rats.

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    authors: China SP,Pal S,Chattopadhyay S,Porwal K,Kushwaha S,Bhattacharyya S,Mittal M,Gurjar AA,Barbhuyan T,Singh AK,Trivedi AK,Gayen JR,Sanyal S,Chattopadhyay N

    更新日期:2017-12-01 00:00:00

  • Intravenous olpadronate restores ovariectomy-affected bone strength. A mechanical, densitometric and tomographic (pQCT) study.

    abstract::Female Wistar rats aged 3 months were ovariectomized (OX, n = 27). Three months later they were given i.v. doses of 150 (6), 300 (7), or 600 (6) ug/kg 2/wk of olpadronate during 12 weeks or left as OX controls (OXc). Bending fracture load of femur diaphyses, reduced in OXc, was recovered by olpadronate. This effect wa...


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    authors: Cointry GR,Mondelo N,Zanchetta JR,Montuori E,Ferretti JL

    更新日期:1995-10-01 00:00:00

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    pub_type: 杂志文章


    authors: Puntila E,Kröger H,Lakka T,Honkanen R,Tuppurainen M

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    pub_type: 杂志文章


    authors: Dodds RA,Emery RJ,Klenerman L,Chayen J,Bitensky L

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    authors: Jordan GR,Loveridge N,Bell KL,Power J,Dickson GR,Vedi S,Rushton N,Clarke MT,Reeve J

    更新日期:2003-01-01 00:00:00

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    authors: Lu X,Ito Y,Atsawasuwan P,Dangaria S,Yan X,Wu T,Evans CA,Luan X

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    authors: Briesacher BA,Andrade SE,Yood RA,Kahler KH

    更新日期:2007-11-01 00:00:00

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    authors: Miller SC,Marks SC Jr

    更新日期:1993-05-01 00:00:00

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    pub_type: 杂志文章,评审


    authors: Ralston SH,Helfrich MH

    更新日期:1999-05-01 00:00:00

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    pub_type: 杂志文章


    authors: Vesterby A,Gundersen HJ,Melsen F,Mosekilde L

    更新日期:1989-01-01 00:00:00

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    authors: Kido S,Kuriwaka-Kido R,Imamura T,Ito Y,Inoue D,Matsumoto T

    更新日期:2009-12-01 00:00:00

  • How B cells influence bone biology in health and disease.

    abstract::It is now well established that important regulatory interactions occur between the cells in the hematopoietic, immune and skeletal systems (osteoimmunology). B lymphocytes (B cells) are responsible for the generation and production of antibodies or immunoglobulins in the body. Together with T cells these lymphocytes ...


    pub_type: 杂志文章,评审


    authors: Horowitz MC,Fretz JA,Lorenzo JA

    更新日期:2010-09-01 00:00:00

  • Identification of the type II Na(+)-Pi cotransporter (Npt2) in the osteoclast and the skeletal phenotype of Npt2-/- mice.

    abstract::We previously reported that a type II sodium phosphate (Na(+)-Pi) cotransporter (Npt2) protein is expressed in osteoclasts and that Pi limitation decreases osteoclast-mediated bone resorption in vitro. We also demonstrated that mice homozygous for the disrupted Npt2 gene (Npt2-/-) exhibit a unique age-dependent bone p...


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    authors: Gupta A,Tenenhouse HS,Hoag HM,Wang D,Khadeer MA,Namba N,Feng X,Hruska KA

    更新日期:2001-11-01 00:00:00

  • Bone histomorphometry in transiliac biopsies from 48 normal, healthy men.

    abstract::Investigators and clinicians use bone histomorphometry data from iliac bone biopsies to study bone abnormalities in diseased patients, and to understand the safety and effectiveness of pharmaceutical interventions. This requires access to a high quality normal data-set to be used for comparisons, a resource that has n...


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    authors: Recker RR,Akhter MP,Lappe JM,Watson P

    更新日期:2018-06-01 00:00:00

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    authors: Beller G,Belavý DL,Sun L,Armbrecht G,Alexandre C,Felsenberg D

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    pub_type: 临床试验,杂志文章


    authors: Bloomfield SA,Mysiw WJ,Jackson RD

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    authors: Whyte MP,Zhang F,Wenkert D,McAlister WH,Mack KE,Benigno MC,Coburn SP,Wagy S,Griffin DM,Ericson KL,Mumm S

    更新日期:2015-06-01 00:00:00

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    pub_type: 杂志文章


    authors: Su Y,Wang L,Liu X,Yang M,Yi C,Liu Y,Huang P,Guo Z,Yu A,Cheng X,Wu X,Blake GM,Engelke K

    更新日期:2020-10-01 00:00:00

  • Computational finite element bone mechanics accurately predicts mechanical competence in the human radius of an elderly population.

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    authors: Mueller TL,Christen D,Sandercott S,Boyd SK,van Rietbergen B,Eckstein F,Lochmüller EM,Müller R,van Lenthe GH

    更新日期:2011-06-01 00:00:00

  • RUNX2 alleles associated with BMD in Scottish women; interaction of RUNX2 alleles with menopausal status and body mass index.

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    authors: Vaughan T,Reid DM,Morrison NA,Ralston SH

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    authors: Iwaniec UT,Magee KA,Mitova-Caneva NG,Wronski TJ

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    authors: Ma L,Duan CC,Yang ZQ,Ding JL,Liu S,Yue ZP,Guo B

    更新日期:2019-06-01 00:00:00

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    pub_type: 杂志文章


    authors: Vom Scheidt A,Grisolia Seifert EF,Pokrant C,Püschel K,Amling M,Busse B,Milovanovic P

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    authors: Katoh-Fukui Y,Baba T,Sato T,Otake H,Nagakui-Noguchi Y,Shindo M,Suyama M,Ohkawa Y,Tsumura H,Morohashi KI,Fukami M

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    authors: Sasaki T,Watanabe C

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    pub_type: 杂志文章


    authors: Olkku A,Bodine PV,Linnala-Kankkunen A,Mahonen A

    更新日期:2004-02-01 00:00:00

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Reppe S,Stilgren L,Olstad OK,Brixen K,Nissen-Meyer LS,Gautvik KM,Abrahamsen B

    更新日期:2006-07-01 00:00:00