Abstract：
:The goal of this study was to identify potential transcriptomic markers in developing asthma by an integrative analysis of multiple public microarray data sets. Using the R software and bioconductor packages, we performed a statistical analysis to identify differentially expressed (DE) genes in asthma, and further performed functional interpretation (enrichment analysis and co-expression network construction) and classification quality evaluation of the DE genes identified. 3 microarray datasets (192 cases and 91 controls in total) were collected for this analysis. 62 DE genes were identified in asthma, among which 43 genes were up-regulated and 19 genes were down-regulated. The up-regulated gene with the highest Log2 Fold Change (LFC) was CLCA1 (LFC=2.81). The down-regulated gene with the highest absolute LFC was BPIFA1 (LFC=-1.45). Enrichment analysis revealed that those DE genes strongly associated with proteolysis, retina homeostasis, humoral immune response, and salivary secretion. A support vector machine classifier (asthma versus healthy control) was also trained based on DE genes. In conclusion, the consistently DE genes identified in this study are suggested as candidate transcriptomic markers for asthma diagnosis, and provide novel insights into the pathogenesis of asthma.
journal_name
Mol Immunoljournal_title
Molecular immunologyauthors
Fang F,Pan J,Li Y,Li Y,Feng X,Wang Jdoi
10.1016/j.molimm.2017.09.021subject
Has Abstractpub_date
2017-12-01 00:00:00pages
38-44eissn
0161-5890issn
1872-9142pii
S0161-5890(17)30520-5journal_volume
92pub_type
杂志文章abstract：:Lysozyme is an important component of the insect non-specific immune response against bacteria that is characterized by its ability to break down bacterial cell-walls. By searching an EST database from the fall armyworm, Spodoptera frugiperda (Negre et al., 2006), we identified five sequences encoding proteins of the ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.09.028
更新日期：2009-12-01 00:00:00
abstract：:The complement system is a vital component of the immune-priveliged human eye that is always active at a low-grade level, preventing harmful intraocular injuries caused by accumulation of turnover products and controlling pathogens to preserve eye homeostasis and vision. The complement system is a double-edged sword t...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2017.05.028
更新日期：2017-09-01 00:00:00
abstract：:Concanavalin A (Con A)section-binding proteins from mouse spleen leukocytes were characterised by two-dimensional electrophoresis of material precipitated, by Con A plus anti-Con A, from lysates of biosynthetically-labelled cells. Although most cell surface (iodinatable) proteins are known to bind Con A, some of the m...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90030-5
更新日期：1983-04-01 00:00:00
abstract：:When human serum is diluted and pre-incubated at 37 degrees C in low ionic strength buffer (LIS, u = 0.07; made iso-osmotic with dextrose), a spontaneous activation of complement (C) is observed as determined by C4 and C3 electrophoretic conversion. In this paper it is postulated that most species of glycosaminoglycan...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90074-x
更新日期：1986-08-01 00:00:00
abstract：:Kappa light chains were investigated in sera of several groups of wild derived mouse strains by radioimmunological analysis using xenogeneic anti-mouse kappa light chain antibodies. Although the majority of strains tested expressed serologically indistinguishable kappa chains, several significant exceptions were obser...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90068-4
更新日期：1986-08-01 00:00:00
abstract：:The molecular interactions between the components of the C3 convertase of the alternative pathway (AP) of complement and its regulators, in both surface-bound and fluid-phase form, are still incompletely understood. The fact that the AP convertase is labile makes studies difficult to perform. According to the so calle...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2007.11.003
更新日期：2008-04-01 00:00:00
abstract：BACKGROUND:Angioedema diagnosis classically targets the complement system (via C1 inhibitor (C1Inh) function and antigenic C4 level) and contact phase activation (via amidase activity). Bradykinin is responsible for angioedema attacks and is produced from contact phase activation secondary to failed C1Inh control. OBJ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.01.028
更新日期：2017-05-01 00:00:00
abstract：:Clonal idiotypic immunoglobulins of follicular lymphomas can be isolated by somatic fusion procedures. Idiotypic IgMs (Id-IgM) were isolated from two patients and used to immunise a strain of mice, deficient in mouse antibody production and engineered with yeast artificial chromosomes (YAC) containing fragments of the...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.03.035
更新日期：2004-07-01 00:00:00
abstract：:The porcine complement proteins C3 and C4 have been isolated and then characterized using small-angle scattering methods. Within the limits of experimental errors, the porcine proteins are virtually identical with the corresponding human proteins as measured in terms of mol. wt, Mr and radius of gyration, R,: Mr(C3) =...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(91)90181-i
更新日期：1991-09-01 00:00:00
abstract：:The 3-D complex structures of extracellular domain of human CD16 (FcgammaRIII) and hIgG1 or B88-9 were obtained by means of computer-guided molecular modeling, respectively. The binding epitopes were predicted and binding energy was calculated theoretically. The epitopes of human CD16 interacted with hIgG1 and B88-9 w...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.03.024
更新日期：2004-05-01 00:00:00
abstract：:Immunoglobulins (Ig) require correct folding and assembly of both heavy (H) and light (L) chains to form a functional H2L2 dimer that is secreted from plasma cells. This process is dependent upon the endoplasmic reticulum (ER) chaperone BiP, which targets improperly, folded or assembled Ig molecules for degradation. W...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2004.11.015
更新日期：2005-05-01 00:00:00
abstract：:Syngeneic polyclonal antibodies were elicited to an affinity labeled high affinity (2-3 X 10(10) M-1) anti-fluorescein murine IgG2a monoclonal antibody. Hyperimmune ascites fluid was tested for reactivity with homologous liganded, affinity labeled and non-liganded Fab fragments derived from the high affinity antibody....
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90111-3
更新日期：1988-08-01 00:00:00
abstract：:Pulmonary surfactant proteins, SP-A and SP-D, are carbohydrate pattern recognition molecules of innate immunity, which significantly enhance phagocytosis and killing of Aspergillus fumigatus, a pathogenic fungus, by neutrophils and macrophages. The present study examined the susceptibility of immunosuppressed SP-A gen...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2010.02.027
更新日期：2010-06-01 00:00:00
abstract：:An initial bioinformatics investigation followed by cloning and sequencing analysis, has led to the identification of three novel members (omDB-2, omDB-3, omBD-4) of the beta-defensin family in rainbow trout (Oncorhynchus mykiss). The contiguous sequences could be translated to give predicted peptides of 62 (omDB-2), ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2009.07.018
更新日期：2009-10-01 00:00:00
abstract：:Type III interferons (IFN-λs) comprise a group of newly identified antiviral cytokines that are functionally similar to type I IFNs and elicit first-line antiviral responses. Recently, type III IFNs were identified in several species; however, little information is available about type III IFNs in ducks. We compared t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.10.013
更新日期：2015-12-01 00:00:00
abstract：:Four monoclonal antibodies (MAbs) directed against the P1 blood group antigen were produced by hybridomas obtained from mouse immunized with turtle-dove avomucoid. One of the MAb (154 IX B6) selected as a blood typing reagent agglutinated native P1 and Pk1 red cells with a high titer but was inactive against native P2...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(87)90089-7
更新日期：1987-02-01 00:00:00
abstract：:Staphylococcus aureus is an etiological agent of human and animal diseases, and it is able to internalize into non-professional phagocytic cells (i.e. bovine mammary epithelial cells, bMECs), which is an event that is related to chronic and recurrent infections. bMECs contribute to host innate immune responses (IIR) t...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2015.09.025
更新日期：2015-12-01 00:00:00
abstract：:A panel of hybridomas producing antibodies specific for human growth hormone (hGH) were prepared by using a recombinant hGH [methionylsomatotropin (r-hGH)] as an immunogen. Thirteen representative monoclonal antibodies which showed different reactivity patterns were used to analyze the antigenicities of four different...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90099-4
更新日期：1986-06-01 00:00:00
abstract：:The complement (C) inflammatory cascade is part of the phylogenetically ancient innate immune response and is crucial to our natural ability to ward off infection. It has three critical physiologic activities: (i) defending against microbial infections by triggering the generation of a membranolytic complex (C5b9 comp...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2004.06.011
更新日期：2004-11-01 00:00:00
abstract：:Major histocompatibility complex (MHC) class I and II proteins have been in the focus of interest for immunologists, biochemists, cell biologists, and structural biologists for decades. With dozens of entries in the Protein Data Bank, their crystal structures are now sufficiently well understood, while their dynamic p...
journal_title：Molecular immunology
pub_type： 杂志文章,评审
doi：10.1016/j.molimm.2009.10.008
更新日期：2010-01-01 00:00:00
abstract：:LMIR5/CD300b is an activating immunoglobulin-like receptor whose extracellular domain (LMIR5-Fc) is constitutively released from immune cells. The release of LMIR5-Fc is augmented upon stimulation with TLR agonists. LMIR5-Fc is reported to possess inflammatory activity and amplify LPS-induced lethal inflammation; howe...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2014.06.012
更新日期：2014-11-01 00:00:00
abstract：:Helminths, as well as their secretory/excretory products, induce a tolerogenic immune microenvironment. High molecular weight components (PI) from Ascaris suum extract down-modulate the immune response against ovalbumin (OVA). The PI exerts direct effect on dendritic cells (DCs) independent of TLR 2, 4 and MyD88 molec...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2017.03.015
更新日期：2017-07-01 00:00:00
abstract：:The transcription factor NF-kappaB plays critical roles in immune and inflammatory responses. Here we show that filarial parasitic sheath proteins cause activation of NF-kappaB in the airway epithelial HEp-2 cell line. This activation was transient and saturable, and involved degradation of the cytoplasmic inhibitor p...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/s0161-5890(00)00039-0
更新日期：2000-02-01 00:00:00
abstract：:Many biological properties of immune complexes (IC) depend upon their size. Quasi-elastic light scattering (QLS) was used to measure a mean equivalent hydrodynamic radius (Rh) and variance of the distribution of model IC composed of bovine serum albumin (BSA) as antigen (Ag) and combinations of two or three well-chara...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(88)90086-7
更新日期：1988-01-01 00:00:00
abstract：:In an attempt to understand the relationship of amino acid sequence to the formation of primary or multiple myeloma-related amyloid (AL amyloid), we have determined the complete amino acid sequence of amyloid protein BAN. This protein belongs to the kappa I immunoglobulin light chain subgroup and has a polypeptide cha...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(86)90173-2
更新日期：1986-01-01 00:00:00
abstract：:Mammals have evolved a special cellular mechanism for killing invading microbes, which is called the phagocytosis. Neutrophils are the first phagocytosing cells that migrate into the site of infection. In these cells, hypochlorite (HOCl) and other hypohalites, generated in the myeloperoxidase (MPO)-hydrogen peroxide (...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.09.017
更新日期：2019-12-01 00:00:00
abstract：:The T-dependent BALB/c murine immune response to fluorescein (F1) is characterized by structural heterogeneity at the protein level exemplified in part by a significantly wide range of affinities (Ka), and apparent lack of dominant idiotypes. In order to generate an idiotype family, xenogenic anti-idiotype (anti-ID) a...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(85)90072-0
更新日期：1985-08-01 00:00:00
abstract：:Purified human C5 was converted non-enzymically to an activated form as defined by its ability to participate in reactive lysis. This conversion occurred following exposure to systems that generate oxygen radicals, namely addition of H2O2 in the presence of ascorbic acid and iron or the addition of xanthine oxidase, a...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(89)90057-6
更新日期：1989-12-01 00:00:00
abstract：:Neutrophil migration is essential for battling against infections but also drives chronic inflammation. Since primary neutrophils are terminally differentiated and not genetically tractable, leukemia cells such as HL-60 are differentiated into neutrophil-like cells to study mechanisms underlying neutrophil migration. ...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/j.molimm.2019.06.001
更新日期：2019-08-01 00:00:00
abstract：:This report demonstrates that a marker of human embryonic endoderm and embryonal carcinoma cells recognized by a hybridoma antibody FC 10.2, involves Type 1 blood group chains with the sequence Gal beta 1 leads to 3G1cNAc beta 1 leads to 3Gal beta 1 leads to 4G1c. This conclusion has been reached from antigenic analys...
journal_title：Molecular immunology
pub_type： 杂志文章
doi：10.1016/0161-5890(83)90005-6
更新日期：1983-06-01 00:00:00
