Rabbit Model of Corneal Endothelial Injury Established Using the Nd: YAG Laser.

Abstract:

PURPOSE:To develop a new rabbit model of corneal endothelial injury using a neodymium-doped yttrium aluminum garnet (Nd:YAG) laser. METHODS:The corneal endothelia of 2 groups of New Zealand white rabbits were treated with an Nd:YAG laser in a uniformly scattered fashion. Rabbits in group A underwent laser burns on the whole corneal endothelium, including the limbus area, whereas rabbits in group B were subjected to laser burns in the central 9-mm diameter zone of the endothelium. Slit-lamp biomicroscopy, optical coherence tomography, applanation tonometry, confocal microscopy, scanning electron microscopy, and histological examinations were performed during 4 weeks of follow-up. RESULTS:In both groups, dotted or focal corneal endothelium defects were directly observed. The stroma was intact. Right after laser application, a series of clinical manifestations appeared, including subepithelial and stromal edema, increased central corneal thickness, and corneal opacity. Laser burn had more notable effects in group A than in group B. In both groups, we observed no damage to the intraocular structures, and intraocular pressure was normal after laser treatment. CONCLUSIONS:Nd:YAG laser treatment in a 9-mm diameter zone of the endothelium can effectively induce bullous keratopathy in a rabbit, whereas treatment for the entire corneal endothelium maintains bullous keratopathy for a longer period. The procedure is simple and reproducible, and it retains normal intraocular structures. This study provided a promising model for future research into endothelial cell damage and for the development of new therapies.

journal_name

Cornea

journal_title

Cornea

authors

Zhang W,Hu Y,Lu L,Liu Y,Yang X,Sun H,Ruan J,Chen J,Yao Q,Yan C,Gu P,Fu Y,Shao C,Fan X

doi

10.1097/ICO.0000000000001324

subject

Has Abstract

pub_date

2017-10-01 00:00:00

pages

1274-1281

issue

10

eissn

0277-3740

issn

1536-4798

journal_volume

36

pub_type

杂志文章

相关文献

CORNEA文献大全
  • Topographic patterns in refractive surgery candidates.

    abstract:PURPOSE:To evaluate the prevalence of different topographic patterns in refractive surgery candidates and to estimate the extent of candidate rejection based on topography alone. METHODS:We performed a retrospective evaluation of videokeratographies of previously unoperated refractive surgery candidates. The defined t...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/01.ico.0000121699.74077.f0

    authors: Varssano D,Kaiserman I,Hazarbassanov R

    更新日期:2004-08-01 00:00:00

  • Effects of diquafosol sodium eye drops on tear film stability in short BUT type of dry eye.

    abstract:PURPOSE:To investigate the effects of diquafosol sodium (DQS) eye drops, a purinergic P2Y2 receptor agonist, on tear film stability in patients with unstable tear film (UTF). METHODS:Two prospective studies were conducted. One was an exploratory nonrandomized trial on 39 eyes with dry eye symptoms and short tear film ...

    journal_title:Cornea

    pub_type: 杂志文章,随机对照试验

    doi:10.1097/ICO.0b013e3182930b1d

    authors: Shimazaki-Den S,Iseda H,Dogru M,Shimazaki J

    更新日期:2013-08-01 00:00:00

  • Endothelial Keratoplasty Update 2020.

    abstract::Endothelial keratoplasty has revolutionized the treatment of corneal endothelial dysfunction and lowered the threshold for treatment by providing rapid visual rehabilitation and setting a high standard for safety and efficacy. Over time, endothelial keratoplasty techniques have evolved toward the use of thinner tissue...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000002565

    authors: Price MO,Feng MT,Price FW Jr

    更新日期:2020-11-30 00:00:00

  • Six different mutations of TGFBI (betaig-h3, keratoepithelin) gene found in Japanese corneal dystrophies.

    abstract:PURPOSE:To investigate mutations of the human transforming growth factor beta-induced gene (TGFBI), transforming growth factor-beta-induced gene product (betaig-h3, keratoepithelin), in Japanese patients with Avellino corneal dystrophy (ACD), lattice corneal dystrophy (LCD), granular corneal dystrophy (GCD), and Reis-B...

    journal_title:Cornea

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验

    doi:10.1097/00003226-200011000-00015

    authors: Fujiki K,Hotta Y,Nakayasu K,Yamaguchi T,Kato T,Uesugi Y,Ha NT,Endo S,Ishida N,Lu WN,Kanai A

    更新日期:2000-11-01 00:00:00

  • Combined use of subconjunctival and intracorneal bevacizumab injection for corneal neovascularization.

    abstract:PURPOSE:To report on the safety and clinical use of combined subconjunctival and intracorneal bevacizumab for corneal neovascularization. METHODS:The charts of 12 consecutive patients with corneal neovascularization who received combined subconjunctival and intracorneal injections of bevacizumab (2.5 mg/0.1 mL) were r...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e31821379aa

    authors: Yeung SN,Lichtinger A,Kim P,Amiran MD,Slomovic AR

    更新日期:2011-10-01 00:00:00

  • Corneal elevation topography: best fit sphere, elevation distance, asphericity, toricity, and clinical implications.

    abstract:PURPOSE:To describe the effect of the corneal asphericity and toricity on the map patterns and best fit sphere (BFS) characteristics in elevation topography. METHODS:The corneal surface was modeled as a biconic surface of principal radii and asphericity values of r1 and r2 and Q1 and Q2, respectively. The apex of the ...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e3181fb4fa7

    authors: Gatinel D,Malet J,Hoang-Xuan T,Azar DT

    更新日期:2011-05-01 00:00:00

  • Donor-related risk factors and preoperative recipient-related risk factors for graft failure.

    abstract:PURPOSE:The aim of this study was to evaluate the outcome of penetrating keratoplasties, at the University Eye Hospital, Ludwig-Maximilians-University, Munich, Germany, using organ-cultured donor corneas and to identify preoperative risk factors, which may influence the event of graft failure. METHODS:In this study, 3...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000000225

    authors: Yu AL,Kaiser M,Schaumberger M,Messmer E,Kook D,Welge-Lussen U

    更新日期:2014-11-01 00:00:00

  • Immunofluorescence of rabbit corneas after collagen cross-linking treatment with riboflavin and ultraviolet A.

    abstract:PURPOSE:To assess ultrastructural modifications in keratocytes and inflammatory cell response in rabbit corneas after riboflavin and ultraviolet A exposure using immunofluorescence microscopy. METHODS:Twenty adult New Zealand albino rabbits weighing 2.0–3.0 kg were used in this study. Two rabbits served as controls.Th...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e3181bdf1cc

    authors: Esquenazi S,He J,Li N,Bazan HE

    更新日期:2010-04-01 00:00:00

  • Determining Factors for Fast Corneal Sensitivity Recovery After Pterygium Excision.

    abstract:PURPOSE:To establish determining factors for fast corneal sensitivity (CS) recovery after pterygium excision. METHODS:Thirty-two eyes of 14 males and 18 females with primary nasal pterygium were recruited. Differences in CS (in the 4 quadrants and the center using a Cochet-Bonnet esthesiometer), pterygium corneal area...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000000932

    authors: Julio G,Campos P,Pujol P,Munguia A,Mas-Aixala E

    更新日期:2016-12-01 00:00:00

  • Anterior-Segment Optical Coherence Tomography and Scanning Electron Microscopy to Evaluate Corneal Epithelial Changes in Patients Undergoing Glaucoma Therapy.

    abstract:PURPOSE:To measure corneal epithelial thickness (CET) in patients with glaucoma using anterior-segment optical coherence tomography and to evaluate CET changes in relation to corneal epithelial microvilli analyzed by scanning electron microscopy (SEM). METHODS:Twenty-two eyes (16 patients) being treated with preservat...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000001752

    authors: Cennamo G,Montorio D,Del Prete S,Del Prete A,Cennamo G

    更新日期:2018-12-01 00:00:00

  • Inhibition of pathological corneal neovascularization by a small peptide derived from human apolipoprotein (a) Kringle V.

    abstract:PURPOSE:The aim of this study was to evaluate the antiangiogenic activity of AU6, a novel 6-amino acid peptide derived from Kringle V of human apolipoprotein (a). METHODS:RF/6A rhesus macaque choroid endothelial cells were used for in vitro studies. MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sul...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000000032

    authors: Wang Z,Zhao H,Ma JX,Xu X

    更新日期:2014-04-01 00:00:00

  • Ultraviolet A/riboflavin corneal cross-linking for infectious keratitis associated with corneal melts.

    abstract:PURPOSE:To evaluate the efficacy of ultraviolet-corneal cross-linking (CXL) for treating infectious melting keratitis. METHODS:Five patients with infectious keratitis associated with corneal melting were treated with CXL at the outpatient departments of the Institut für Refraktive und Ophthalmo-Chirurgie and the eye h...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e318169d698

    authors: Iseli HP,Thiel MA,Hafezi F,Kampmeier J,Seiler T

    更新日期:2008-06-01 00:00:00

  • Efficacy of plasma rich in growth factors for the treatment of dry eye.

    abstract:PURPOSE:To evaluate the efficacy of plasma rich in growth factors (PRGF) for the treatment of moderate/severe dry eye. METHODS:PRGF treatment was administered to 16 patients who had moderate/severe dry eye diagnosed and who had not responded previously to other standard treatments. We quantified several growth factors...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e31820d86d6

    authors: López-Plandolit S,Morales MC,Freire V,Grau AE,Durán JA

    更新日期:2011-12-01 00:00:00

  • Inhibitory effects of tranilast on the proliferation and functions of human pterygium-derived fibroblasts.

    abstract:PURPOSE:We studied the possibility that tranilast, an antiallergic and antiproliferative drug, may be beneficial for the treatment of pterygium. METHODS:Pterygium-derived cells were identified by immunohistochemical methods. Growth rate of pterygium-derived cells was determined by using a hemocytometer. Chemotaxis was...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/00003226-200005000-00021

    authors: Isaji M,Kikuchi S,Miyata H,Ajisawa Y,Araki-Inazawa K,Tsukamoto Y,Amano Y

    更新日期:2000-05-01 00:00:00

  • Intraoperative mitomycin C and corneal endothelium after pterygium surgery.

    abstract:PURPOSE:To determine the effect of mitomycin C (MMC) on the corneal endothelium after primary pterygium surgery. METHODS:This prospective, interventional, nonrandomized, observer-masked study included 46 consecutive patients (51 eyes) with primary pterygium. The bare sclera technique with 1-minute application of 0.02%...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e3181a3c548

    authors: Pérez-Rico C,Benítez-Herreros J,Montes-Mollón MA,Germain F,Castro-Rebollo M,Gómez-SanGil Y,Paz-Moreno J,Teus MA

    更新日期:2009-12-01 00:00:00

  • Herpes simplex virus keratitis: histopathologic neovascularization and corneal allograft failure.

    abstract:PURPOSE:To identify whether histopathologic presence of neovascularization is predictive for allograft failure following penetrating keratoplasty for herpes simplex virus (HSV) keratitis. METHODS:Retrospective, interventional case series of 62 consecutive patients with HSV keratitis who underwent penetrating keratopla...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e31819c4e55

    authors: Garcia DD,Shtein RM,Musch DC,Elner VM

    更新日期:2009-10-01 00:00:00

  • Indications for penetrating keratoplasty and associated procedures, 1989-1995.

    abstract:PURPOSE:To identify changing trends in indications for penetrating keratoplasty and associated surgical procedures. METHODS:Review of charts from all patients who underwent penetrating keratoplasty at Wills Eye Hospital from January 1, 1989 through December 31, 1995. RESULTS:A total of 2,442 corneal transplants were ...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:

    authors: Lois N,Kowal VO,Cohen EJ,Rapuano CJ,Gault JA,Raber IM,Laibson PR

    更新日期:1997-11-01 00:00:00

  • Correlation of corneal elevation with severity of keratoconus by means of anterior and posterior topographic analysis.

    abstract:PURPOSE:To investigate the severity of keratoconus in terms of corneal elevation differences with 2 different reference surfaces using topographic analysis. METHODS:Eighty-six eyes of 50 patients with keratoconus of various clinical stages (61 male and 25 female eyes) with mean age of 37.3 ± 11.6 years were evaluated....

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0B013E31823D1EE0

    authors: Ishii R,Kamiya K,Igarashi A,Shimizu K,Utsumi Y,Kumanomido T

    更新日期:2012-03-01 00:00:00

  • Diabetes mellitus increases risk of unsuccessful graft preparation in Descemet membrane endothelial keratoplasty: a multicenter study.

    abstract:PURPOSE:The aim of this study was to evaluate preparation outcomes of tissue prepared for Descemet membrane endothelial keratoplasty (DMEK) from diabetic and nondiabetic donors. METHODS:In this nonrandomized, consecutive case series, DMEK grafts were prepared from diabetic and nondiabetic donors by experienced technic...

    journal_title:Cornea

    pub_type: 杂志文章,多中心研究

    doi:10.1097/ICO.0000000000000262

    authors: Greiner MA,Rixen JJ,Wagoner MD,Schmidt GA,Stoeger CG,Straiko MD,Zimmerman MB,Kitzmann AS,Goins KM

    更新日期:2014-11-01 00:00:00

  • Effect of topical cyclosporin A on herpetic stromal keratitis in a mouse model.

    abstract:PURPOSE:To study the effect of topical cyclosporin A on herpetic stromal keratitis (HSK) in a mouse model. METHODS:The corneas of BALB/c mice were infected with herpes simplex type 1 virus. The mice were divided into 4 groups according to topical treatment methods: vehicle solution, 0.01% cyclosporin A, 0.1% cyclospor...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e318160602d

    authors: Yoon KC,Heo H,Kang IS,Lee MC,Kim KK,Park SH,Cho KO

    更新日期:2008-05-01 00:00:00

  • Femtosecond laser-assisted intracorneal biopolymer insertion for the symptomatic treatment of bullous keratopathy.

    abstract:PURPOSE:To describe a new minimally invasive surgical technique for the symptomatic management of bullous keratopathy in blind eyes. METHODS:Four patients with severe corneal edema due to endothelial decompensation and no visual function in the affected eye presented for the relief of their ocular symptoms (pain and t...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000000081

    authors: Kymionis GD,Diakonis VF,Kankariya VP,Plaka AD,Panagopoulou SI,Kontadakis GA,Grentzelos MA,Tsilimbaris MK,Pallikaris IG

    更新日期:2014-05-01 00:00:00

  • Modified relaxing incision technique for postkeratoplasty astigmatism.

    abstract::A modified relaxing incision technique for postkeratoplasty astigmatism is described in this article. Following the initial, standard relaxing incision techniques, a planned spreading and recutting of the initial relaxing incision was performed at 1-3-week intervals following the initial surgery. The wound may be deep...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:

    authors: Forstot SL

    更新日期:1988-01-01 00:00:00

  • Diagnosis of Aspergillus keratitis in vivo with confocal microscopy.

    abstract::Confocal microscopy provides a new, noninvasive way of imaging the human cornea in vivo. One of its most important clinical uses is the diagnosis and management of infectious keratitis. The authors used tandem scanning confocal microscopy to image the corneas in two culture-proven cases of Aspergillus keratitis. Funga...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:

    authors: Winchester K,Mathers WD,Sutphin JE

    更新日期:1997-01-01 00:00:00

  • Candida albicans interface infection after deep anterior lamellar keratoplasty.

    abstract:PURPOSE:To report a case of interface infection by Candida albicans after deep anterior lamellar keratoplasty (DALK). METHODS:A 30-year-old man with keratoconus underwent DALK. Four weeks after surgery, the patient developed multiple infiltrates in the graft-host interface with absence of intraocular infection. Donor ...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0b013e318074e475

    authors: Fontana L,Parente G,Di Pede B,Tassinari G

    更新日期:2007-08-01 00:00:00

  • A Novel Mobile Application for Preoperative and Intraoperative Assessments of Intrastromal Corneal Ring Segments Implantation for Keratoconus.

    abstract:PURPOSE:To develop a mobile app that allows photographs to be captured and edited under the overlay of images and projection of a protractor with 360 degrees axis markings, which enables the surgeon to accurately visualize the programmed and marked alignment where the intrastromal corneal ring segments (ICRS) will be i...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000002385

    authors: Fernandes Dias FA,Fernandes Dias VJ,Carneiro CLB,Bernardes BG,Dutra BAL,Crispim Ribeiro J

    更新日期:2020-09-01 00:00:00

  • The effect of age on the corneal subbasal nerve plexus.

    abstract:PURPOSE:To measure subbasal nerve density and orientation in normal human corneas across a broad age range. METHODS:Sixty-five normal corneas of 65 subjects were examined by using tandem scanning confocal microscopy. Ages of subjects ranged from 15 to 79 years (mean 46 +/- 19 years), with 5 subjects from each hemideca...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/01.ico.0000154387.51355.39

    authors: Erie JC,McLaren JW,Hodge DO,Bourne WM

    更新日期:2005-08-01 00:00:00

  • A New Approach for the Calculation of Total Corneal Astigmatism Considering the Magnitude and Orientation of Posterior Corneal Astigmatism and Thickness.

    abstract:PURPOSE:To evaluate a new method of calculation of total corneal astigmatism based on Gaussian optics and the power design of a spherocylindrical lens (C) in the healthy eye and to compare it with keratometric (K) and power vector (PV) methods. METHODS:A total of 92 healthy eyes of 92 patients (age, 17-65 years) were ...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000001577

    authors: Piñero DP,Caballero MT,Nicolás-Albujer JM,de Fez D,Camps VJ

    更新日期:2018-06-01 00:00:00

  • Fungal Keratitis Caused by Exserohilum, An Emerging Pathogen.

    abstract:PURPOSE:To determine the epidemiology, risk factors, clinical features, and prognosis of fungal keratitis caused by Exserohilum. METHODS:All culture-proven cases of keratomycosis caused by Exserohilum presented from January 2012 to December 2014 were retrospectively analyzed using a computerized database. RESULTS:Amo...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000000774

    authors: Rathi H,Venugopal A,Rameshkumar G,Ramakrishnan R,Meenakshi R

    更新日期:2016-05-01 00:00:00

  • Autologous Descemet Stripping Automated Endothelial Keratoplasty to Eliminate Endothelial Rejection in Eyes at High Risk.

    abstract:PURPOSE:To determine whether autologous Descemet stripping endothelial keratoplasty is technically feasible and whether it eliminates the risk of endothelial rejection in "only eyes" at high risk of immunological rejection. METHODS:This is a prospective observational interventional study from 2016 to 2018 with a 24-mo...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000002184

    authors: Myerscough J,Friehmann A,Bovone C,Busin M

    更新日期:2020-05-01 00:00:00

  • Corneal Subbasal Nerve Density and Sensitivity After Pars Plana Vitrectomy Using Contact or Noncontact Wide-Angle Viewing Systems.

    abstract:PURPOSE:To assess corneal subbasal nerve density (SBND) and corneal sensitivity (CS) after pars plana vitrectomy (PPV) for rhegmatogenous retinal detachment (RRD) without intraoperative 360-degree laser treatment using contact or noncontact panoramic viewing systems. METHODS:This is a prospective study of 34 eyes affe...

    journal_title:Cornea

    pub_type: 杂志文章

    doi:10.1097/ICO.0000000000001656

    authors: Tosi GM,Bacci T,Tarantello A,Martone G,Traversi C,Marigliani D,Cevenini G,Virgili G

    更新日期:2018-09-01 00:00:00