Effect of topical cyclosporin A on herpetic stromal keratitis in a mouse model.

Abstract:

PURPOSE:To study the effect of topical cyclosporin A on herpetic stromal keratitis (HSK) in a mouse model. METHODS:The corneas of BALB/c mice were infected with herpes simplex type 1 virus. The mice were divided into 4 groups according to topical treatment methods: vehicle solution, 0.01% cyclosporin A, 0.1% cyclosporin A, and 1% cyclosporin A. The severity of stromal keratitis was graded clinically at 3, 7, 10, and 14 days after treatment. Histologic and immunohistochemical examinations were performed to analyze infiltrating inflammatory cells and T lymphocytes at 14 days after treatment. Flow cytometry was performed to count CD4 T cells at 14 days after treatment. RESULTS:On day 3 after treatment, there were no significant differences in mean severity scores of HSK between the vehicle and cyclosporin A-treated groups. On days 7, 10, and 14, the severity scores in the groups treated with 0.1% or 1% cyclosporin A decreased significantly compared with the vehicle group (P < 0.05). However, the scores in the group treated with 0.01% cyclosporin A did not change up to 14 days after treatment. By histologic and immunohistochemical analysis, a significant decrease in the total number of inflammatory cells and T lymphocytes was detected in the groups treated with 0.1% or 1% cyclosporin A. Flow cytometry also showed similar findings. CONCLUSIONS:Topical cyclosporin A effectively reduces stromal haze and inflammation in experimental HSK, and cyclosporin A eyedrops with a >0.1% concentration can be used for the treatment of HSK.

journal_name

Cornea

journal_title

Cornea

authors

Yoon KC,Heo H,Kang IS,Lee MC,Kim KK,Park SH,Cho KO

doi

10.1097/ICO.0b013e318160602d

subject

Has Abstract

pub_date

2008-05-01 00:00:00

pages

454-60

issue

4

eissn

0277-3740

issn

1536-4798

pii

00003226-200805000-00012

journal_volume

27

pub_type

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