Antitumor Macrophage Response to Bacillus pumilus Ribonuclease (Binase).

Abstract:

:Extracellular bacterial ribonucleases such as binase from Bacillus pumilus possess cytotoxic activity against tumor cells with a potential for clinical application. Moreover, they may induce activation of tumor-derived macrophages either into the M1-phenotype with well-documented functions in the regulation of the antitumor immune response or into M2-macrophages that may stimulate tumor growth, metastasis, and angiogenesis. In this study, binase or endogenous RNase1 (but not RNA or short oligonucleotides) stimulated the expression of activated NF-κB p65 subunit in macrophages. Since no changes in MyD88 and TRIF adaptor protein expression were observed, toll-like receptors may not be involved in RNase-related NF-κB pathway activation. In addition, short exposure (0.5 hr) to binase induced the release of cytokines such as IL-6, МСР-1, or TNF-α (but not IL-4 and IL-10), indicative for the polarization into antitumor M1-macrophages. Thus, we revealed increased expression of activated NF-κB p65 subunit in macrophages upon stimulation by binase and RNase1, but not RNA or short oligonucleotides.

journal_name

Mediators Inflamm

authors

Makeeva A,Rodriguez-Montesinos J,Zelenikhin P,Nesmelov A,Preissner KT,Cabrera-Fuentes HA,Ilinskaya ON

doi

10.1155/2017/4029641

subject

Has Abstract

pub_date

2017-01-01 00:00:00

pages

4029641

eissn

0962-9351

issn

1466-1861

journal_volume

2017

pub_type

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