Abstract:
:This study aimed to isolate rabbit adipose-derived stem cells (ADSCs) and explore the potential of platelet-rich plasma (PRP) in the chondrogenic differentiation of ADSCs, thereby potentially providing a new approach for the repair and regeneration of cartilage injury. Rabbit ADSCs were isolated and characterized by induction towards adipogenic, osteogenic and chondrogenic lineages in vitro. The isolated ADSCs were also cultured with or without 10% PRP. Immunofluorescence staining, toluidine blue staining and reverse transcription-quantitative polymerase chain reaction (RT-qPCR) were used to detect type II collagen (Col II) and aggrecan (AGC) expression. Col II immunofluorescence staining and toluidine blue staining indicated that following induction by autologous PRP, ADSCs manifested Col II and AGC expression. The expression of Col II and AGC mRNA was significantly upregulated in the PRP-treated cells when compared with that in control cells. Autologous PRP produced by laboratory centrifugation was able to promote the chondrogenic differentiation of rabbit ADSCs in vitro.
journal_name
Exp Ther Medjournal_title
Experimental and therapeutic medicineauthors
Tang XB,Dong PL,Wang J,Zhou HY,Zhang HX,Wang SZdoi
10.3892/etm.2015.2528subject
Has Abstractpub_date
2015-08-01 00:00:00pages
477-483issue
2eissn
1792-0981issn
1792-1015pii
ETM-0-0-2528journal_volume
10pub_type
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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abstract::The aim of the present study was to investigate whether the co-injection of hyaluronic acid (HA) and corticosteroids (CS) was superior to HA alone in the treatment of knee OA. A total of 120 participants with symptomatic knee OA were recruited and formed the intention-to-treat population for a 6-month follow-up. In th...
journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2018.6371
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2013.1395
更新日期:2014-01-01 00:00:00
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doi:10.3892/etm.2018.6664
更新日期:2018-11-01 00:00:00
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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abstract::The present study aimed to explore the treatment effects of Taohongsiwu decoction on the liver fibrosis in a rat model, as few prior studies have investigated the anti-fibrotic effects of Taohongsiwu decoction. High-performance liquid chromatography was used to measure the concentration of ferulic acid and hydrosafflo...
journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章,评审
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更新日期:2020-08-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
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更新日期:2011-03-01 00:00:00
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
doi:10.3892/etm.2017.5089
更新日期:2017-11-01 00:00:00