当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Long non-coding RNA PVT1 promotes glycolysis and tumor progression by regulating miR-497/HK2 axis in osteosarcoma.

Long non-coding RNA PVT1 promotes glycolysis and tumor progression by regulating miR-497/HK2 axis in osteosarcoma.

Abstract：

:Cancer cells usually utilize glucose as a carbon source for aerobic glycolysis, a phenomenon known as the Warburg effect. Increasing studies have shown that PVT1 (a long non-coding RNA, lncRNA) functions as critical regulator of multiple cancers. However, it remains totally unknown whether and how PVT1 regulates glucose metabolism in OS cells. In this study, we found that the expression of PVT1 were specifically increased in OS cells and tissues, and the upregulated PVT1 indicated poor prognosis. Glucose uptake, lactate production, and the expression of HK2 in OS cells were increased by overexpression of PVT1, while decreased by PVT1 knockdown. We further fund that PVT1 acted as molecular sponge to repress miR-497. Inhibition of miR-497 promoted glucose consumption and lactate production, phenomenon could be reversed by PVT1 silencing. Moreover, HK2 was a direct target of miR-497 and overexpression of HK2 attenuated the suppressive effect of miR-497 on glycolysis. Functionally, knockdown of PVT1 exerted tumor-suppressive effect by suppressing cell proliferation, cell cycle progression, and invasion in vitro, whereas miR-497 inhibitor partially abolished the inhibition effect of si-PVT1. Overexpression of HK2 attenuated the miR-497 induced inhibition of cell growth and motility. Taken together, these findings suggested that PVT1 contributes to OS cell glucose metabolism, cell proliferation, and motility through the miR-497/HK2 pathway, and revealed a novel relation between lncRNA and the alteration of glycolysis in OS cells.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Song J,Wu X,Liu F,Li M,Sun Y,Wang Y,Wang C,Zhu K,Jia X,Wang B,Ma X

doi

10.1016/j.bbrc.2017.06.024

subject

Has Abstract

pub_date

2017-08-19 00:00:00

pages

217-224

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(17)31143-9

journal_volume

490

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Identification and cloning of a second phytase gene (phyB) from Aspergillus niger (ficuum).

    abstract：:An Aspergillus niger (ficuum) genomic DNA lambda EMBL3 library was probed with a 354-bp DNA fragment obtained by polymerase chain reaction of A. niger DNA with oligonucleotides based on partial amino acid sequence of a pH 2.5 optimum acid phosphatase. A clone containing a 1605 bp segment (phyB) encoding the 479 amino ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1993.2008

    authors： Ehrlich KC,Montalbano BG,Mullaney EJ,Dischinger HC Jr,Ullah AH

    更新日期：1993-08-31 00:00:00

  • Phospholipase C mimics tumor promoter-induced chemiluminescence in murine epidermal cells.

    abstract：:Treatment of newborn murine epidermal cells with phospholipase C results in the generation of a chemiluminescence response similar to that previously described for phorbol ester tumor promoters. Based on inhibitor studies, the oxidant, believed to be superoxide anion, is most likely generated from lipoxygenase metabol...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(85)90204-9

    authors： Fischer SM,Baldwin JK,Adams LM

    更新日期：1985-09-30 00:00:00

  • Tumor necrosis factor-alpha gene is expressed in stimulated retinal pigment epithelial cells in culture.

    abstract：:Expression of TNF-alpha gene in the retinal pigment epithelial (RPE) cells was studied by using polymerase chain reaction (PCR). PCR for human TNF-alpha gene using complementary DNA (cDNA) of control RNA prepared from non-stimulated RPE cells failed to show expression of TNF-alpha gene. However, PCR by using cDNA prep...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)91300-f

    authors： Tanihara H,Yoshida M,Yoshimura N

    更新日期：1992-09-16 00:00:00

  • hKSR-2 inhibits MEKK3-activated MAP kinase and NF-kappaB pathways in inflammation.

    abstract：:Kinase suppressor of ras (KSR) and MEKK3 (MAP kinase kinase kinase) are integral members of the MAP kinase pathway. We have recently identified a new isoform of the KSR family named human kinase suppressor of ras-2 (hKSR-2), and demonstrated that hKSR-2 negatively regulates Cot, a MAP3K family member which is importan...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.07.009

    authors： Channavajhala PL,Rao VR,Spaulding V,Lin LL,Zhang YG

    更新日期：2005-09-09 00:00:00

  • Pyrrolidine dithiocarbamate prevents IL-1-induced nitric oxide synthase mRNA, but not superoxide dismutase mRNA, in insulin producing cells.

    abstract：:We presently investigated the effects of pyrrolidine dithiocarbamate (PDTC), a potent inhibitor of nuclear factor kappa B (NF-kappa B), on the induction of nitric oxide synthase (iNOS) and manganese superoxide dismutase (MnSOD) mRNAs by IL-1 beta in insulin-producing RIN cells. PDTC decreased by 90% both IL-1 beta-ind...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1995.1731

    authors： Bedoya FJ,Flodström M,Eizirik DL

    更新日期：1995-05-25 00:00:00

  • MINK is a Rap2 effector for phosphorylation of the postsynaptic scaffold protein TANC1.

    abstract：:Rap1 and Rap2 are similar Ras-like G proteins but perform distinct functions. By the affinity chromatography/mass-spectrometry approach and the yeast two-hybrid screening, we identified Misshapen/NIKs-related kinase (MINK) as a novel Rap2-interacting protein that does not interact with Rap1 or Ras. MINK is a member of...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.10.038

    authors： Nonaka H,Takei K,Umikawa M,Oshiro M,Kuninaka K,Bayarjargal M,Asato T,Yamashiro Y,Uechi Y,Endo S,Suzuki T,Kariya KI

    更新日期：2008-12-12 00:00:00

  • Systemic Klotho therapy protects against insulitis and enhances beta-cell mass in NOD mice.

    abstract：:The levels of the anti-aging protein α-Klotho, in its soluble form (s-Klotho), are depressed in the circulation of patients with type 1 diabetes (T1D) or type 2 diabetes (T2D). Gene transfer experiments have suggested a protective role for β-cell specific expression of α-Klotho in murine models of T1D and T1D, but the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2020.02.123

    authors： Prud'homme GJ,Glinka Y,Kurt M,Liu W,Wang Q

    更新日期：2020-05-07 00:00:00

  • Purification, molecular cloning and functional characterization of swine phosphatidylethanolamine-binding protein 4 from seminal plasma.

    abstract：:Phosphatidylethanolamine-binding proteins (PEBPs) are found in various species and have multiple functions. In this study, we purified the swine homolog of human PEBP4 (sPEBP4) from swine seminal plasma, cloned the sPEBP4 cDNA and functionally characterized this protein. The molecular mass of the purified protein was ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.06.016

    authors： An LP,Maeda T,Sakaue T,Takeuchi K,Yamane T,Du PG,Ohkubo I,Ogita H

    更新日期：2012-07-13 00:00:00

  • Potentiation by chronic ethanol treatment of the mitochondrial permeability transition.

    abstract：:Mitochondria isolated from rats chronically fed ethanol were more sensitive to induction of the mitochondrial permeability transition (MPT) by a variety of agents than mitochondria isolated from isocalorically matched controls. The agents utilized have been implicated in both necrotic (Ca(2+)) and apoptotic (ceramide,...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1696

    authors： Pastorino JG,Marcineviciute A,Cahill A,Hoek JB

    更新日期：1999-11-19 00:00:00

  • Molecular cloning of anti-SS-A/Ro 60-kDa peptide Fab fragments from infiltrating salivary gland lymphocytes of a patient with Sjögren's syndrome.

    abstract：:Anti-SS-A/Ro antibodies are commonly found in systemic autoimmune diseases such as Sjögren's syndrome and systemic lupus erythematosus, and some of these antibodies appear to be responsible for certain pathological lesions including congenital heart block in neonatal lupus. In this study, we generated three human anti...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.6233

    authors： Suzuki H,Takemura H,Suzuki M,Sekine Y,Kashiwagi H

    更新日期：1997-03-06 00:00:00

  • Molecular tilting in dried elastoidin and its implications for the structures of other collagen fibrils.

    abstract：:Wet elastoidin spicules (fish fin rays) yield low-angle meridional X-ray diffraction patterns which resemble those from tendons. However, when the spicule dries the meridian splits into the arms of a diagonal cross (sometimes only one arm appears). Of the possible explanations we reject shearing of the axial arrangeme...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(76)90229-1

    authors： Hukins DW,Woodhead-Galloway J,Knight DP

    更新日期：1976-12-20 00:00:00

  • Analysis of the cytokine-stimulated human inducible nitric oxide synthase (iNOS) gene: characterization of differences between human and mouse iNOS promoters.

    abstract：:Expression of human inducible nitric oxide synthase (hiNOS) is under cytokine control and is transcriptionally regulated. The hiNOS and mouse iNOS (miNOS) genes are regulated differently by cytokines. To understand better the transcriptional regulation of the hiNOS gene, the 8.3-kb hiNOS promoter was characterized. Pr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9062

    authors： Chu SC,Marks-Konczalik J,Wu HP,Banks TC,Moss J

    更新日期：1998-07-30 00:00:00

  • Phosphatidylinositol 3-kinase in HL-60 nuclei is bound to the nuclear matrix and increases during granulocytic differentiation.

    abstract：:We have used HL-60 leukemia cells to investigate phosphatidylinositol 3-kinase (PI 3-K) during granulocytic differentiation at the nuclear level. Nuclei of HL-60 cells showed a constitutive presence of PI 3-K that increased when cells were treated with differentiating doses of ATRA. PI 3-K was also detected tightly bo...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.9787

    authors： Marchisio M,Bertagnolo V,Colamussi ML,Capitani S,Neri LM

    更新日期：1998-12-18 00:00:00

  • Effect of matrix metalloproteinase inhibition on adipose tissue development.

    abstract：:The effect of Ro 28-2653, a synthetic matrix metalloproteinase (MMP) inhibitor, on adipose tissue development was studied in mice kept on a high fat diet (HFD). Five-week-old male wild-type (C57Bl/6J) mice were fed the HFD (42% kcal as fat, 20.1 kJ/g) and received daily p.o. instillations of inhibitor (30 mg/kg) or ve...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.01.103

    authors： Demeulemeester D,Collen D,Lijnen HR

    更新日期：2005-04-01 00:00:00

  • NADH-ferric reductase activity associated with dihydropteridine reductase.

    abstract：:In mammals dietary ferric iron is reduced to ferrous iron for more efficient absorption by the intestine. Analysis of a pig duodenal membrane fraction revealed two NADH-dependent ferric reductase activities, one associated with a b-type cytochrome and the other not. Purification and characterization of the non-cytochr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2708

    authors： Lee PL,Halloran C,Cross AR,Beutler E

    更新日期：2000-05-19 00:00:00

  • Disruption of the mu-delta opioid receptor heteromer.

    abstract：:The crystal structure of the mu and kappa opioid receptors has revealed dimeric structural arrangements. Mu-delta receptors heteromers also exist and we have identified discrete cytoplasmic regions in each receptor required for oligomer formation. In the carboxyl tail of the delta receptor we identified three glycine ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.05.023

    authors： O'Dowd BF,Ji X,O'Dowd PB,Nguyen T,George SR

    更新日期：2012-06-15 00:00:00

  • High-pressure infrared spectroscopic study of human proinsulin gene expression in live Escherichia coli cells.

    abstract：:Infrared spectra of E. coli strain JM103 and transformants which overproduced recombinant proinsulin have been measured as a function of pressure up to 38 kbar. It is the first time that high-pressure infrared spectra of live bacteria have been successfully measured. In ambient conditions, spectra of the host strain J...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90715-7

    authors： Wong PT,Zahab DM,Narang SA,Sung WL

    更新日期：1987-07-15 00:00:00

  • Functional evidence that the self-renewal gene NANOG regulates esophageal squamous cancer development.

    abstract：:Cancer cell molecular mimicry of stem cells (SC) follows with enhanced proliferative and renewal capacities. In support, numerous mediators of SC self-renewal have been evinced to exhibit oncogenic potential. More and more researches showed that the embryonic stem cell self-renewal genes express in various cancer cell...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.06.016

    authors： Deng L,Xiang X,Yang F,Xiao D,Liu K,Chen Z,Zhang R,Feng G

    更新日期：2017-08-19 00:00:00

  • TT virus is distributed in various leukocyte subpopulations at distinct levels, with the highest viral load in granulocytes.

    abstract：:When TT virus (TTV) DNA was quantitated in whole blood and plasma aliquots from 27 viremic individuals by real-time detection PCR that can detect essentially all TTV genotypes, the TTV load was 6.9 +/- 3.5 (mean +/- standard deviation)-fold higher in the whole blood than in the plasma samples [P < 0.002 (paired t test...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2001.6183

    authors： Takahashi M,Asabe S,Gotanda Y,Kishimoto J,Tsuda F,Okamoto H

    更新日期：2002-01-11 00:00:00

  • Regulation of stretch-activated intracellular calcium transients by actin filaments.

    abstract：:Stretch activation of cation-permeable channels may be an important proximal sensory mechanism in mechanotransduction. As actin filaments may mediate cellular responses to changes of the mechanical properties of the substrate and regulate stretch-induced calcium transients, we examined the role of actin filaments and ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1057

    authors： Wu Z,Wong K,Glogauer M,Ellen RP,McCulloch CA

    更新日期：1999-08-02 00:00:00

  • Okadaic acid interferes with lipoprotein-supported corticosterone production in adrenal cells.

    abstract：:Rat adrenocortical cells in culture respond to stimulation by ACTH alone (15 fold over basal) and to ACTH + added lipoproteins (as an exogeneous source of cholesterol), with an additional 25-30 fold rise in steroidogenesis. With the addition of okadaic acid (OKA, 100 nM), a potent protein phosphatase inhibitor, the li...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(91)91877-f

    authors： Azhar S,Wang H,Tsai L,Reaven E

    更新日期：1991-09-16 00:00:00

  • Establishment of a new glial cell line from hippocampus of prion protein gene-deficient mice.

    abstract：:Cellular prion protein (PrP(C)) is expressed not only in neuronal cells but also in non-neuronal cells such as astroglial cells. In the present study, the prion protein (PrP) gene (Prnp)-deficient astroglial cell line GpL1 from hippocampal cells of ZrchI Prnp(-/-) mice were established. Transfection of Prnp suppressed...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.10.087

    authors： Nishimura T,Sakudo A,Xue G,Ikuta K,Yukawa M,Sugiura K,Onodera T

    更新日期：2008-12-26 00:00:00

  • Two novel monoclonal antibodies to fibronectin that recognize the Hep II and CS-1 regions respectively: their differential effect on lymphocyte adhesion.

    abstract：:We have obtained two new mAbs to the carboxy-terminal region of fibronectin, namely P3D4 and P1F11, and have studied their binding sites and their ability to block lymphocyte adhesion to fibronectin. ELISA and Western blot analyses showed that P3D4 reacts with both fibronectin chains and both Hep II-containing fragmen...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)80785-5

    authors： Garcia-Pardo A,Sánchez-Aparicio P,Wayner EA

    更新日期：1992-07-15 00:00:00

  • The mechanism of action of DuP 721, a new antibacterial agent: effects on macromolecular synthesis.

    abstract：:Pulse labeling studies with Bacillus subtilis showed that DuP 721 inhibited protein synthesis. The IC50 of DuP 721 for protein synthesis was 0.25 micrograms/ml but it was greater than 32 micrograms/ml for RNA and DNA synthesis. In cell-free systems, DuP 721 concentrations up to 100 microM did not inhibit peptide chain...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(88)90723-1

    authors： Eustice DC,Feldman PA,Slee AM

    更新日期：1988-02-15 00:00:00

  • Hexose transport in plasma membrane vesicles prepared from L6 rat myoblasts.

    abstract：:Hexose transport in plasma membrane vesicles prepared from L6 rat myoblasts was shown to be stereospecific, activated by glucose starvation and occurred by both high and low affinity systems. Transport by the high affinity system was shown to occur by an active transport process. Furthermore, the high affinity system ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(86)80343-6

    authors： Mesmer O,Cheung MO,D'Amore T,Lo TC

    更新日期：1986-11-26 00:00:00

  • Sphingosine-1-phosphate induces human endothelial VEGF and MMP-2 production via transcription factor ZNF580: novel insights into angiogenesis.

    abstract：:Sphingosine-1-phosphate (S1P)-induced migration and proliferation of endothelial cells are critical for angiogenesis. C2H2-zinc finger (ZNF) proteins usually play an essential role in altering gene expression and regulating the angiogenesis. The aim of this study is to investigate whether a novel human C2H2-zinc finge...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.04.019

    authors： Sun HY,Wei SP,Xu RC,Xu PX,Zhang WC

    更新日期：2010-05-07 00:00:00

  • Action of Bauhinia bauhinioides synthetic peptides on serine proteinases.

    abstract：:The kallikrein inhibitor found in Bauhinia bauhinioides seeds (BbKI) differs from classical Kunitz plant inhibitors in the lack of disulfide bridges in its structure [Biochim. Biophys. Acta 1477 (2000) 64-74]. In this study, we examined whether structural properties may be involved in inhibitory specificity and, if so...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2003.09.203

    authors： Cagliari CI,De Caroli FP,Nakahata AM,Araújo MS,Nakaie CR,Sampaio MU,Sampaio CA,Oliva ML

    更新日期：2003-11-07 00:00:00

  • Specific binding of leukotriene B4 to guinea pig lung membranes.

    abstract：:We have demonstrated binding sites for LTB4 in guinea pig lung membranes. Binding of [3H]-LTB4 was of high affinity (Kd = 0.76 nM), saturable and linear with protein concentration (0.2-1.2 mg/ml). Scatchard and Hill's plot analysis indicated a single class of binding site with a Hill's coefficient of 0.99 +/- 0.08 (n ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(87)90674-7

    authors： Saad M,Wong K

    更新日期：1987-02-27 00:00:00

  • Construction of a cDNA clone containing the entire coding region of the human liver-type phosphofructokinase.

    abstract：:Genomic fragments containing coding sequences of the human liver phosphofructokinase (PFKL) were used to screen cDNA libraries and several clones corresponding to PFKL were isolated. Three overlapping cDNA clones spanning the entire coding region were stitched together yielding the plasmid pG-cPFKL3.0 containing a 3Kb...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(87)80194-8

    authors： Levanon D,Danciger E,Dafni N,Groner Y

    更新日期：1987-09-30 00:00:00

  • TNF-α knockdown alleviates palmitate-induced insulin resistance in C2C12 skeletal muscle cells.

    abstract：:Insulin resistance is a cardinal feature of Type 2 Diabetes (T2D), which accompanied by lipid accumulation and TNF-α overexpression in skeletal muscle. The role of TNF-α in palmitate-induced insulin resistance remained to be elucidated. Here, we assessed effects of TNF-α knockdown on the components of insulin signalin...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.03.137

    authors： Haghani K,Pashaei S,Vakili S,Taheripak G,Bakhtiyari S

    更新日期：2015-05-15 00:00:00