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Rapid purification of inositol monophosphate phosphatase from beef brain.

Abstract：

:A procedure is described for preparation of homogeneous inositol monophosphate phosphatase (EC 3.1.3.25) from beef brain in less than 2 days with an overall recovery of 15-25%. This enzyme, an essential part of the inositol phospholipid cycle in brain, is a proposed site of action of lithium ions in manic-depressive disorders. The major purification steps are: a) removal of most interfering protein by heat denaturation at 75 degrees C for 1 h, b) separation by anion exchange at a pH (6.0) near the enzyme's pI (4.9), and c) adsorption of most remaining impurities on a Procion Red affinity column.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Meek JL,Rice TJ,Anton E

doi

10.1016/s0006-291x(88)80816-7

subject

Has Abstract

pub_date

1988-10-14 00:00:00

pages

143-8

issue

1

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(88)80816-7

journal_volume

156

pub_type

杂志文章

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