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A new assay based on terminal restriction fragment length polymorphism of homocitrate synthase gene fragments for Candida species identification.

Abstract：

:Candida sp. have been responsible for an increasing number of infections, especially in patients with immunodeficiency. Species-specific differentiation of Candida sp. is difficult in routine diagnosis. This identification can have a highly significant association in therapy and prophylaxis. This work has shown a new application of the terminal restriction fragment length polymorphism (t-RFLP) method in the molecular identification of six species of Candida, which are the most common causes of fungal infections. Specific for fungi homocitrate synthase gene was chosen as a molecular target for amplification. The use of three restriction enzymes, DraI, RsaI, and BglII, for amplicon digestion can generate species-specific fluorescence labeled DNA fragment profiles, which can be used to determine the diagnostic algorithm. The designed method can be a cost-efficient high-throughput molecular technique for the identification of six clinically important Candida species.

journal_name

J Appl Genet

journal_title

Journal of applied genetics

authors

Szemiako K,Śledzińska A,Krawczyk B

doi

10.1007/s13353-017-0394-5

subject

Has Abstract

pub_date

2017-08-01 00:00:00

pages

409-414

issue

3

eissn

1234-1983

issn

2190-3883

pii

10.1007/s13353-017-0394-5

journal_volume

58

pub_type

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