Induction and characterization of streptomycin-resistant mutants in Capsicum praetermissum.

Abstract:

:Streptomycin-resistant mutants were isolated from mutagenised cotyledon explants of Capsicum praetermissum Heiser & Smith. The explants were mutagenised with N-ethyl-N-nitrosourea, which resulted in a high frequency of streptomycin-resistant mutants (18.0%) and a low frequency of chlorophyll-deficient (albino) mutants (8.0%). Complete streptomycin-resistant plantlets were obtained after rooting of the regenerated green shoots on rooting medium containing 1.0 mg L-1 IAA and 500 mg L-1 streptomycin sulphate. Leaf-segment assay of these plantlets revealed that they were resistant to streptomycin but sensitive to chloramphenicol, kanamycin, lincomycin, and spectinomycin. Reciprocal crosses between streptomycin-resistant and -sensitive plants showed a non-Mendelian transmission of resistance by female parents.

journal_name

J Appl Genet

authors

Venkataiah P,Christopher T,Subhash K

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

19-24

issue

1

eissn

1234-1983

issn

2190-3883

pii

243

journal_volume

46

pub_type

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