Abstract:
:The ErbB signalling network plays a crucial role in the growth and progression of several cancers, including colorectal cancer (CRC), and includes potentially drug-targetable genes. Oncogenic activation of the ErbB pathway by mutations and focal amplifications have emerged recently as an important predictive marker of the prognosis of CRC patients. However, in contrast to genetic events, little is known about epigenetic alternations of ErbB-associated genes and their impact on gene expression. Genome-wide methylation in sporadic CRCs (n = 12) paired with adjacent normal tissues have been previously analysed by Illumina Infinium HumanMethylation27 (HM27) at 27,578 CpG sites. For confirmation of our initial genome-wide analysis, we used a published HM27 dataset (GSE25062). Subsequently, CpG island methylation of selected ErbB pathway-associated genes was assessed on 233 CRC samples using methylation-sensitive polymerase chain reaction (MS-PCR) and analysed along with various genetic factors associated with CRC [epigenotype, BRAF and KRAS mutations, microsatellite instability (MSI)]. Methylation and expression integration was performed using published datasets including 25 pairs of CRC and normal colon tissues (GSE25062 and GSE25070), and confirmed with real-time PCR. Our previous microarray-based genome-wide DNA methylation analysis of 12 CRCs revealed that four ErbB-associated genes (PIK3CD, PKCΒ, ERBB4, ) were differentially methylated in CRCs. This was further confirmed by statistical re-analysis of an HM27 dataset (GSE25062). Frequent methylation at these loci in tumours was subsequently confirmed by MS-PCR (63%, 43%, 43% and 92%, respectively). Hypermethylation of PKCΒ associated with KRAS mutation (p = 0.04), whereas hypermethylation of ERBB4 associated with high-methylation epigenotypes (HME), BRAF mutation and MSI (p = 0.001, 0.002 and 0.0002, respectively). One of the four analysed genes (PKCΒ) was significantly downregulated in CRC tissue, as revealed by real-time PCR and re-analysis of the GSE25062 and GSE25070 datasets. After careful re-analysis of published methylation and expression data, we conclude that methylation of ERBB4, PAK7 and PIK3CD has no functional role in CRC carcinogenesis. In contrast, methylation seems to have a potential impact on the biology of colorectal tumours by negatively modulating the expression of PKCΒ. Importantly, the relationship between DNA methylation of PKCΒ and gene expression may warrant further attention in the context of colon cancer chemoprevention and anti-cancer therapy.
journal_name
J Appl Genetjournal_title
Journal of applied geneticsauthors
Szmida E,Karpiński P,Leszczynski P,Sedziak T,Kielan W,Ostasiewicz P,Sasiadek MMdoi
10.1007/s13353-014-0253-6subject
Has Abstractpub_date
2015-05-01 00:00:00pages
185-92issue
2eissn
1234-1983issn
2190-3883journal_volume
56pub_type
杂志文章abstract::Progress in mammalian cloning started from cloning embryos (of mice, rats, rabbits, sheep, goats, pigs, cattle and rhesus monkeys) and culminated in obtaining clones of sheep, cattle, pigs and mice from adult somatic cells. Knowing the relationship between the cell cycles of the recipient and the donor of cell nucleus...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
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journal_title:Journal of applied genetics
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journal_title:Journal of applied genetics
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更新日期:2001-01-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
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journal_title:Journal of applied genetics
pub_type: 杂志文章
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更新日期:2011-11-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
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更新日期:2003-01-01 00:00:00
abstract::Phenylketonuria (PKU) is a metabolic disease caused by mutations in the phenylalanine hydroxylase (PAH) gene. Although the PAH genotype remains the main determinant of PKU phenotype severity, genotype-phenotype inconsistencies have been reported. In this study, we focused on unanalysed sequences in non-coding PAH gene...
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journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03194627
更新日期:2006-01-01 00:00:00
abstract::Fluorescent in situ hybridization (FISH) was performed in 76 patients referred to our department because of intellectual disability and dysmorphic features that can be related to subtelomeric microaberrations. In all the patients, conventional cytogenetic methods revealed normal karyotype. Four (5.3%) subtelomeric rea...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03195731
更新日期:2010-01-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03195679
更新日期:2009-01-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Hereditary hearing loss (HHL) is a neurosensory disorder that affects every 1/500 newborns worldwide and nearly 1/3 people over the age of 65. Congenital deafness is inherited as monogenetic or polygenic disorder. The delicacy, tissue heterogeneity, deep location of the inner ear down the brainstem, and minute quantit...
journal_title:Journal of applied genetics
pub_type: 杂志文章,评审
doi:10.1007/s13353-019-00535-6
更新日期:2020-02-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-020-00562-8
更新日期:2020-09-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章,评审
doi:10.1007/s13353-013-0134-4
更新日期:2013-05-01 00:00:00
abstract::Philomycus bilineatus is a highly common gastropod mollusk pest in China and is also utilized to treat infectious diseases. However, no genomic resources are available for this non-model species. In the present study, the transcriptomic analysis of P. bilineatus was completed. After sequencing using the next generatio...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-020-00566-4
更新日期:2020-09-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-011-0051-3
更新日期:2011-11-01 00:00:00
abstract::The glyceraldehyde-3-phosphate dehydrogenase promoter of the food yeast Candida utilis strain NRRL Y-660 was cloned to create a novel integrative vector for Agrobacterium tumefaciens-mediated transformation. The new binary vector harbors β-glucuronidase activity as reporter and kanamicin/geneticin resistance as select...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-013-0162-0
更新日期:2013-11-01 00:00:00
abstract::Differentiated thyroid cancer (DTC) has one of the lowest cancer mutational burdens, while anaplastic thyroid cancer (ATC) has a much higher mutation frequency. A fraction of ATC has an associated differentiated component, which suggests the coevolution of both cancers. Here, we aimed to compare mutation frequency in ...
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pub_type: 杂志文章
doi:10.1007/s13353-020-00594-0
更新日期:2021-02-01 00:00:00
abstract::Based on segregation distortion of simple sequence repeat (SSR) molecular markers, we detected a significant quantitative trait loci (QTL) for pre-harvest sprouting (PHS) tolerance on the short arm of chromosome 2D (2DS) in the extremely susceptible population of F2 progeny generated from the cross of PHS tolerant syn...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03195631
更新日期:2008-01-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:
更新日期:2001-01-01 00:00:00
abstract::The mechanisms of root hair formation have been studied extensively in Arabidopsis but knowledge about these processes in monocot species is still limited, especially in relation to the proteome level. The aim of this study was to identify the proteins that are involved in the initiation and the early stage of root ha...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-012-0105-1
更新日期:2012-11-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03195642
更新日期:2008-01-01 00:00:00
abstract::A rye doubled haploid (DH) mapping population (Amilo × Voima) segregating for pre-harvest sprouting (PHS) was generated through anther culture of F(1) plants. A linkage map was constructed using DHs, to our knowledge, for the first time in rye. The map was composed of 289 loci: amplified fragment length polymorphism (...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-011-0029-1
更新日期:2011-08-01 00:00:00
abstract::The long QT syndrome (LQTS) is an inherited cardiac arrhythmia that may lead to sudden death in the absence of structural heart disease. Mutations in the cardiac potassium and sodium channel genes can be found in approximately 70 percent of patients with a highly probable clinical diagnosis. In this study, we aimed to...
journal_title:Journal of applied genetics
pub_type: 杂志文章
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更新日期:2010-01-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-017-0408-3
更新日期:2017-11-01 00:00:00
abstract::The long QT syndrome (LQTS) is a disorder of ventricular repolarization that exposes affected individuals to cardiac arrhythmias and sudden death. The first gene for LQTS has been mapped to chromosome 11 p.15.5 by genome-wide linkage analysis. This gene, originally named KVLQT1 (and later KCNQ1), is a novel potassium ...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::The Rpi-phu1 gene originates from an interspecific hybrid between Solanum stenotomum and S. phureja, and confers a high level of resistance to Phytophthora infestans (late blight) in potato. The Rpi-phu1 was introduced by crossing at the diploid level into the S. tuberosum gene pool and then transferred to the tetrapl...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03195721
更新日期:2010-01-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
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更新日期:2011-02-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03194600
更新日期:2006-01-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章,评审
doi:10.1007/s13353-015-0309-2
更新日期:2016-05-01 00:00:00