Abstract:
:The glyceraldehyde-3-phosphate dehydrogenase promoter of the food yeast Candida utilis strain NRRL Y-660 was cloned to create a novel integrative vector for Agrobacterium tumefaciens-mediated transformation. The new binary vector harbors β-glucuronidase activity as reporter and kanamicin/geneticin resistance as selection marker. Recombinant clones of A. tumefaciens show kanamycin resistance and high β-glucuronidase activity under the control of the C. utilis promoter. This finding can be explained by the presence of a prokaryotic core in the yeast promoter, predicted by in silico analysis of the sequence. This is the first report about functionality of a yeast promoter in A. tumefaciens.
journal_name
J Appl Genetjournal_title
Journal of applied geneticsauthors
González T,Eng F,Fraga R,Fonseca J,Amores Idoi
10.1007/s13353-013-0162-0subject
Has Abstractpub_date
2013-11-01 00:00:00pages
495-9issue
4eissn
1234-1983issn
2190-3883journal_volume
54pub_type
杂志文章abstract::Polymorphisms in the coding region of the prion protein gene (PRNP) have been associated with the susceptibility and incubation period of prion diseases in humans and sheep. However, polymorphisms in this part of the bovine PRNP gene do not affect the classical bovine spongiform encephalopathy (BSE) susceptibility in ...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-012-0102-4
更新日期:2012-08-01 00:00:00
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journal_title:Journal of applied genetics
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doi:10.1007/s13353-016-0365-2
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journal_title:Journal of applied genetics
pub_type: 杂志文章,评审
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journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03195710
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journal_title:Journal of applied genetics
pub_type: 杂志文章,评审
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更新日期:2005-01-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章,评审
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更新日期:2008-01-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-015-0328-z
更新日期:2016-08-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03195649
更新日期:2009-01-01 00:00:00
abstract::Differentiated thyroid cancer (DTC) has one of the lowest cancer mutational burdens, while anaplastic thyroid cancer (ATC) has a much higher mutation frequency. A fraction of ATC has an associated differentiated component, which suggests the coevolution of both cancers. Here, we aimed to compare mutation frequency in ...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-020-00594-0
更新日期:2021-02-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-019-00517-8
更新日期:2019-11-01 00:00:00
abstract::The number of functional teats is an important selection criterion in pig breeding. Inherited defects of the udder, such as the inverted teat, do have a considerable negative impact on the nursing ability of the sow. To investigate the genetic background of this defect and the number of functional teats in Swedish mat...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-016-0382-1
更新日期:2017-05-01 00:00:00
abstract::Tumour necrosis factor alpha (TNF-α) is one kind of cytokines which is related to inflammation and lipid metabolism. TNF-α cDNA was cloned from the liver of blunt snout bream (Megalobrama amblycephala) through real-time polymerase chain reaction (PCR) and rapid amplification of cDNA ends (RACE) methods. The full-lengt...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-017-0390-9
更新日期:2017-08-01 00:00:00
abstract::Establishing translocated populations is a common process to preserve and maintain genetic diversity of threatened species. In 2001, three translocated populations of noble crayfish (Astacus astacus) were established in the Czech Republic, founded by either adult or juvenile individuals from three particular source po...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-015-0296-3
更新日期:2016-02-01 00:00:00
abstract::The distinctness, uniformity and stability (DUS) requirements involve expensive, space- and time-consuming measurements of morphological traits. Moreover, for a majority of traits, interactions between genotype and environment complicate the evaluation. Molecular markers have a potential to facilitate this procedure, ...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03195609
更新日期:2008-01-01 00:00:00
abstract::Investigations on the impact of chemicals on the environment and human health have led to the development of an exposome concept. The exposome refers to the totality of exposures received by a person during life, including exposures to life-style factors, from the prenatal period to death. The exposure to genotoxic ch...
journal_title:Journal of applied genetics
pub_type: 杂志文章,评审
doi:10.1007/s13353-016-0380-3
更新日期:2017-08-01 00:00:00
abstract::A simplified AFLP method, based on methylation-sensitive Alw44I restriction endonuclease, has been developed and evaluated for fingerprinting 15 wheat cultivars. The selected germplasms represented groups of spring and winter wheats with and without the 1BL.1RS translocation. Ten selective primers yielded 57 markers, ...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::The matrix metalloproteinase-2 gene (MMP2) was found to be associated with hip structure in pigs. Recently three quantitative trait loci (QTLs) for loin muscle area were found on chromosome 6, to which MMP2 was mapped. In the present study, association analyses were conducted in two pig populations for a single-nucleo...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03195679
更新日期:2009-01-01 00:00:00
abstract::In this study we have developed protocols for induced triploidy and gynogenesis of Senegalese sole (Solea senegalensis), a promising flatfish species for marine aquaculture, in order to: 1) identify the sex-determination mechanism; and 2) to improve its production by generating a) sterile fish, avoiding problems relat...
journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/s13353-014-0233-x
更新日期:2015-02-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03194608
更新日期:2006-01-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
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journal_title:Journal of applied genetics
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journal_title:Journal of applied genetics
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abstract::Philomycus bilineatus is a highly common gastropod mollusk pest in China and is also utilized to treat infectious diseases. However, no genomic resources are available for this non-model species. In the present study, the transcriptomic analysis of P. bilineatus was completed. After sequencing using the next generatio...
journal_title:Journal of applied genetics
pub_type: 杂志文章
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journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
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journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03194645
更新日期:2006-01-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
doi:10.1007/BF03195642
更新日期:2008-01-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
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更新日期:2017-08-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
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更新日期:2006-01-01 00:00:00
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journal_title:Journal of applied genetics
pub_type: 杂志文章
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