The possible role of cell cycle stage in mammalian cloning.

Abstract:

:Progress in mammalian cloning started from cloning embryos (of mice, rats, rabbits, sheep, goats, pigs, cattle and rhesus monkeys) and culminated in obtaining clones of sheep, cattle, pigs and mice from adult somatic cells. Knowing the relationship between the cell cycles of the recipient and the donor of cell nucleus in embryonic cloning by nuclear transfer one can adjust the phases of the cell cycle properly. Metaphase II recipients accept G1 (in most species) or G2 donors (in the mouse). Interphase recipients can harbour nuclei in all stages of cell cycle. Relatively little is known about somatic cloning. Two attitudes are applied: either the donor is in the G0 phase or the recipient is in a prolonged MII phase.

journal_name

J Appl Genet

authors

Modliński JA,Grabarek J,Płusa B,Karasiewicz J

subject

Has Abstract

pub_date

2001-01-01 00:00:00

pages

153-76

issue

2

eissn

1234-1983

issn

2190-3883

pii

37

journal_volume

42

pub_type

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