Apoptosis of the Tracheal Epithelium Can Increase the Number of Recipient Bone Marrow-Derived Myofibroblasts in Allografts and Exacerbate Obliterative Bronchiolitis After Tracheal Transplantation in Mice.


BACKGROUND:The long-term outcome of lung transplantation is impeded by the development of obliterative bronchiolitis (OB). We sought to investigate the relationship between the apoptosis of tracheal epithelial cells and bone marrow-derived myofibroblasts in the process of OB. METHODS:The mouse orthotopic tracheal transplant model was established. The allografts and syngrafts were harvested at 1, 2, 3, and 4 weeks after tracheal transplant. The percentage of tracheal lumen occlusion was assayed via histology and morphometry. Immunofluorescence staining was used to detect the apoptotic epithelial cells and recipient-derived myofibroblasts. The expression of SDF-1α and TGF-β and the infiltrations of CD4 and CD8 T cells in the grafts were detected using immunohistochemical staining. RESULTS:We found that there were more apoptotic epithelia in the allograft group than in the syngraft group and that the level of tracheal lumen occlusion was higher at different time points. Moreover, the increase in the apoptosis of the tracheal epithelium occurred earlier than that of occlusion of the tracheal lumen. There were more myofibroblasts derived from the recipient's bone marrow and more CD4 and CD8 T cells in the allografts. The expression of SDF-1α and TGF-β was higher in the epithelium from allografts than in those of the syngrafts. CONCLUSIONS:Our study indicated that the apoptotic tracheal epithelia in the OB model might increase the amount of myofibroblasts derived from the recipient's bone marrow. Therapeutic methods aimed at preventing apoptosis of the tracheal epithelium may improve the outcome of lung transplantation.






Wang C,Xia T,Jiang K,Qiao X,Zhang X,Li J,Wang J,Nie J




Has Abstract


2016-09-01 00:00:00












  • Duodenal histology for monitoring treatment of acute rejection in pancreaticoduodenal allografts in rats.

    abstract:BACKGROUND:Although the value of duodenal histology as a means to diagnose acute rejection in pancreaticoduodenal allografts has been validated, it is not known how the duodenum responds to antirejection treatment in comparison with the pancreas. METHODS:Diabetic Lewis rats received a pancreaticoduodenal allograft. Cy...


    pub_type: 杂志文章


    authors: Mark W,Hechenleitner P,Dietze O,Klima G,Schneeberger S,Steurer W,Candinas D,Margreiter R,Königsrainer A

    更新日期:2002-01-27 00:00:00

  • Pancreatic islet transplantation: the radiographic approach.

    abstract:BACKGROUND:Successful pancreatic islet transplantation (PIT) has resulted in several transplant centers wanting to start PIT programs. PIT remains experimental and must be performed safely for its continued use. We describe the radiographic techniques used at our center and their results. METHODS:Between January 17, 2...


    pub_type: 杂志文章


    authors: Goss JA,Soltes G,Goodpastor SE,Barth M,Lam R,Brunicardi FC,Froud T,Alejandro R,Ricordi C

    更新日期:2003-07-15 00:00:00

  • Pregnancy after donor nephrectomy.

    abstract::Potential female donors frequently ask whether unilateral nephrectomy will impair future childbearing capabilities. To address this question, we surveyed 220 women who underwent donor nephrectomy between 1985 and 1992. Of the 144 women who responded, 33 became pregnant after donation for a total of 45 pregnancies. Sev...


    pub_type: 杂志文章


    authors: Wrenshall LE,McHugh L,Felton P,Dunn DL,Matas AJ

    更新日期:1996-12-27 00:00:00

  • The Safety of Neuromuscular Blockade Reversal in Patients With Cardiac Transplantation.

    abstract:BACKGROUND:Neuromuscular blockade (NMB) reversal with neostigmine and glycopyrrolate has been reported to cause cardiac arrest in patients with a history of cardiac transplantation. The purpose of this study was to examine the safety of NMB reversal with acetylcholinesterase inhibitors and muscarinic anticholinergics i...


    pub_type: 杂志文章


    authors: Barbara DW,Christensen JM,Mauermann WJ,Dearani JA,Hyder JA

    更新日期:2016-12-01 00:00:00

  • Treatment of patients with recurrent hepatitis C after liver transplantation with peginterferon alfa-2B plus ribavirin.

    abstract:BACKGROUND:Recurrent hepatitis C virus (HCV) after liver transplantation (OLT) is a major cause of graft loss in HCV-positive patients. In this study, we evaluated the efficacy and safety of pegylated interferon alfa-2b (peginterferon) and ribavirin treatment for recurrent HCV after OLT and analyzed the influence of an...


    pub_type: 临床试验,杂志文章


    authors: Neumann U,Puhl G,Bahra M,Berg T,Langrehr JM,Neuhaus R,Neuhaus P

    更新日期:2006-07-15 00:00:00

  • The hemodynamic effects of intraoperative injection of muromonab CD3.

    abstract::During anesthesia 5 mg of muromonab CD3 (OKT3), an anti-CD3 monoclonal antibody, was administered prophylactically to twelve patients undergoing cadaveric renal transplantation. Preoperatively, all patients were at or near their dry body weights. Methylprednisolone 500 mg on call to or in the operating room, azathiopr...


    pub_type: 杂志文章


    authors: Robinson ST,Barry JM,Norman DJ

    更新日期:1993-08-01 00:00:00

  • A novel lymphocyte activation antigen present on allospecific primed lymphocytes and defined by the monoclonal antibody VG01.

    abstract::This study describes VG01, a monoclonal antibody to a novel lymphocyte activation antigen. The cells reacting with VG01 were first characterized by indirect immunofluorescence and fluorescence-activated cell sorter analysis. The antigen defined by VG01 was expressed on circulating T lymphocytes (23.8 +/- 9.0) and on t...


    pub_type: 杂志文章


    authors: Denegri JF,Gilks CB,Altin M,Peterson J,Lafleur L

    更新日期:1988-05-01 00:00:00

  • Escaping from rejection.

    abstract::Since the earliest days of transplantation, immunobiologists have sought means to prevent recognition and rejection of foreign tissue. The goal of these strategies is the retention of recipient immune function while selectively avoiding graft injury. Although considerable theoretical and technical problems remain, an ...


    pub_type: 杂志文章,评审


    authors: Lynch RJ,Platt JL

    更新日期:2009-12-15 00:00:00

  • Computer analysis of cadaver kidney allocation procedures.

    abstract::Data of 32,000 donors were utilized for a computer simulation to analyze the effect of selection parameters on the outcome of kidney transplants. If the HLA match grade is considered for organ allocation, the overall 1-year graft survival rate is up to 7% higher for first cadaver transplants and up to 12% higher for s...


    pub_type: 杂志文章


    authors: Wujciak T,Opelz G

    更新日期:1993-03-01 00:00:00

  • Diarrhea after kidney transplantation: a new look at a frequent symptom.

    abstract::Diarrhea is a frequent but overlooked complication of kidney transplantation. Diarrhea is repeatedly neglected, often considered by patients and clinicians an unavoidable side effect of immunosuppressive regimens. It is, however, associated with a significant impairment in life quality. Severe and chronic posttranspla...


    pub_type: 杂志文章,评审


    authors: Aulagnon F,Scemla A,DeWolf S,Legendre C,Zuber J

    更新日期:2014-10-27 00:00:00

  • 3-[(dodecylthiocarbonyl)methyl]-glutarimide attenuates graft arterial disease by suppressing alloimmune responses and vascular smooth muscle cell proliferation.

    abstract:BACKGROUND:Graft arterial disease (GAD) is a major cause of late graft loss after organ transplantation. Alloimmune responses and vascular remodeling eventually cause the transplant organ to develop GAD. In this study, we aimed to limit the development of GAD by inhibiting alloimmune responses and vascular smooth muscl...


    pub_type: 杂志文章


    authors: Zaitsu M,Yamashita K,Shibasaki S,Tsunetoshi Y,Fukai M,Ogura M,Yoshida T,Igarashi R,Kobayashi N,Umezawa K,Todo S

    更新日期:2015-05-01 00:00:00

  • alpha1,3-Galactosyltransferase-gene knockout in cattle using a single targeting vector with loxP sequences and cre-expressing adenovirus.

    abstract:BACKGROUND:Gene targeting in large animals has the potential to be useful in medicine as well as in agriculture. Previously, we reported the first successful targeting of the bovine alpha1,3-galactosyltransferase (alpha1,3GT) gene and establishment of a heterozygous knockout cell line. In this report, we generated both...


    pub_type: 杂志文章


    authors: Sendai Y,Sawada T,Urakawa M,Shinkai Y,Kubota K,Hoshi H,Aoyagi Y

    更新日期:2006-03-15 00:00:00

  • Drug-Eluting Stents Versus Bare Metal Stents for Percutaneous Coronary Intervention in Kidney Transplant Recipients.

    abstract:BACKGROUND:The comparative effectiveness of percutaneous coronary intervention (PCI) with drug-eluting stents (DES) versus bare metal stents (BMS) has not been studied in the kidney transplant population. METHODS:Using the US Renal Data System, we identified 3245 kidney transplant patients who underwent PCI between Ap...


    pub_type: 杂志文章


    authors: Lenihan CR,Montez-Rath ME,Winkelmayer WC,Chang TI

    更新日期:2017-04-01 00:00:00

  • Sensitization with xenogeneic tissues alters the heavy chain repertoire of human anti-Galalpha1-3Gal antibodies.

    abstract:BACKGROUND:Antigen sensitization alters the use of genes encoding the variable and constant regions of immunoglobulin, changing avidity, and function. Alterations in variable region genes induced by carbohydrate antigens have been studied extensively in animals but are incompletely characterized in humans. We asked how...


    pub_type: 杂志文章


    authors: Yu PB,Parker W,Nayak JV,Platt JL

    更新日期:2005-07-15 00:00:00

  • Analysis of factors affecting recurrence of hepatocellular carcinoma after liver transplantation with a special focus on inflammation markers.

    abstract:BACKGROUND:Systemic inflammation markers, such as neutrophil-to-lymphocyte ratio (NLR), have recently emerged as the prognostic factors for recurrence of liver tumors. METHODS:We assessed the ability of NLR and of other variables to predict the outcomes of orthotopic liver transplantation (OLT) for hepatocellular carc...


    pub_type: 杂志文章


    authors: Bertuzzo VR,Cescon M,Ravaioli M,Grazi GL,Ercolani G,Del Gaudio M,Cucchetti A,D'Errico-Grigioni A,Golfieri R,Pinna AD

    更新日期:2011-06-15 00:00:00

  • Benefits of cytomegalovirus prophylaxis in solid organ transplantation.

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    pub_type: 杂志文章,评审


    authors: Pescovitz MD

    更新日期:2006-07-27 00:00:00

  • Zero-Time Renal Transplant Biopsies: A Comprehensive Review.

    abstract::Zero-time kidney biopsies, obtained at time of transplantation, are performed in many transplant centers worldwide. Decisions on kidney discard, kidney allocation, and choice of peritransplant and posttransplant treatment are sometimes based on the histological information obtained from these biopsies. This comprehens...


    pub_type: 杂志文章,评审


    authors: Naesens M

    更新日期:2016-07-01 00:00:00

  • Safety of anti-hepatitis B core antibody-positive donors for living-donor liver transplantation.

    abstract::Serologic evidence of resolved hepatitis B virus (HBV) infection (HBV surface antigen negative, anti-HBV core antibody [HBc] positive) in a liver donor can be regarded as an occult infection with episomal HBV in the liver. The purpose of this study was to evaluate the safety of anti-HBc-positive living donors. Between...


    pub_type: 杂志文章


    authors: Hwang S,Moon DB,Lee SG,Park KM,Kim KH,Ahn CS,Lee YJ,Chu CW,Yang HS,Cho SH,Oh KB,Ha TY,Min PC

    更新日期:2003-02-15 00:00:00

  • Combined liver-kidney transplantation in primary hyperoxaluria type 1. Bone histopathology and oxalate body content.

    abstract::In three patients with end-stage renal failure due to primary hyperoxaluria type 1, successful combined liver-kidney transplantation enabled us to assess the insoluble oxalate pool, which was compared with the histopathological changes observed in iliac crest biopsy specimens. Good correlation was observed between the...


    pub_type: 杂志文章


    authors: Toussaint C,Vienne A,De Pauw L,Gelin M,Janssen F,Hall M,Schurmans T,Pasteels JL

    更新日期:1995-06-27 00:00:00

  • Early expression of interferon-gamma inducible protein 10 and monokine induced by interferon-gamma in cardiac allografts is mediated by CD8+ T cells.

    abstract:BACKGROUND:Our goal was to test the intragraft mRNA expression and production of two chemokines that are potent chemoattractants for antigen-primed T cells, interferon-gamma inducible protein 10 (IP-10) and monokine-induced by IFN-gamma, (Mig), in allogeneic heart grafts. METHODS:Syngeneic or allogeneic A/J (H-2a) hea...


    pub_type: 杂志文章


    authors: Kapoor A,Morita K,Engeman TM,Koga S,Vapnek EM,Hobart MG,Fairchild RL

    更新日期:2000-03-27 00:00:00

  • Insulin-heparin infusions peritransplant substantially improve single-donor clinical islet transplant success.

    abstract:BACKGROUND:Successful islet transplantation can result in insulin independence in many patients with type 1 diabetes mellitus, but it often requires more than one islet infusion. The ability to achieve insulin independence with a single donor is an important goal in clinical islet transplantation due to the limited org...


    pub_type: 杂志文章


    authors: Koh A,Senior P,Salam A,Kin T,Imes S,Dinyari P,Malcolm A,Toso C,Nilsson B,Korsgren O,Shapiro AM

    更新日期:2010-02-27 00:00:00

  • Direct induction of class II molecules by cytomegalovirus in rat heart microvascular endothelial cells is inhibited by ganciclovir (DHPG).

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    pub_type: 杂志文章


    authors: Ustinov JA,Lahtinen TT,Bruggeman CA,Häyry PJ,Lautenschlager IT

    更新日期:1994-11-15 00:00:00

  • A simplified model for heterotopic rat heart transplantation.

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    pub_type: 杂志文章


    authors: Lee S,Macedo AR,Curtis GP,Lee D,Orloff MJ

    更新日期:1982-04-01 00:00:00

  • Regression of androgen-related hepatic tumors in patients with Fanconi's anemia following marrow transplantation.

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    pub_type: 杂志文章


    authors: Schmidt E,Deeg HJ,Storb R

    更新日期:1984-05-01 00:00:00

  • Atypical mixed lymphocyte culture. Identity between mother and child in leukemia and aplastic anemia.

    abstract::The mixed lymphocyte culture tests between one patient with acute lymphocytic leukemia and her mother and between another patient with aplastic anemia and his mother were found nonreactive, despite a one-haplotype difference for HL-A. Two crossovers in each family at the MLC locus or a specific defect in lymphocyte r...


    pub_type: 杂志文章


    authors: Opelz G

    更新日期:1975-01-01 00:00:00

  • Mediators involved in the immunomodulatory effects of apoptotic cells.

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    pub_type: 杂志文章,评审


    authors: Saas P,Bonnefoy F,Kury-Paulin S,Kleinclauss F,Perruche S

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  • Isolation of large numbers of mesenchymal stem cells from the washings of bone marrow collection bags: characterization of fresh mesenchymal stem cells.

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    pub_type: 杂志文章


    authors: Mageed AS,Pietryga DW,DeHeer DH,West RA

    更新日期:2007-04-27 00:00:00

  • Sequential antilymphoblast globulin and cyclosporine for renal transplantation.

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    pub_type: 杂志文章


    authors: Sommer BG,Henry M,Ferguson RM

    更新日期:1987-01-01 00:00:00

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    pub_type: 杂志文章


    authors: Prümmer O,Raghavachar A,Werner C,Calvo W,Carbonell F,Steinbach I,Fliedner TM

    更新日期:1985-04-01 00:00:00

  • Selection of a suitable internal control gene for expression studies in pancreatic islet grafts.

    abstract::The use of real-time reverse transcription polymerase chain reaction to compare gene expression in different tissues and conditions requires normalization to an internal control that must be expressed at a constant level. Although a previous validation step is required to confirm that an internal control is appropriat...


    pub_type: 杂志文章


    authors: Rodriguez-Mulero S,Montanya E

    更新日期:2005-09-15 00:00:00