Abstract:
:The present study investigated the characteristic features of cancer stem cells (CSCs) using an aggressive human osteosarcoma cell line OS-65. Hoechst 33342 dye exclusion was used to distinguish the cancer stem-like side population (SP) cells from OS-65 cells. Furthermore, the SP cells were characterized via chemoresistance and cell death assays, reverse transcription-quantitative polymerase chain reaction and immunofluorescence. The present study identified ~3.3% of cancer stem-like SP cells from OS-65 cells whose prevalence is reduced significantly (0.9%) following treatment with verapamil. It was demonstrated that osteosarcoma SP cells are highly efficient at generating additional sarcospheres as transcriptional regulation of stemness genes, including SOX2, OCT-4 and NANOG, is highly upregulated. Notably, these SP cells demonstrated high resistance against chemotherapeutic drugs and apoptosis via elevated transcriptional regulation of several ATPase binding cassette (ABC) transporter and anti-apoptotic proteins, including ABCG2, ABCB1/MDR1 ABCB5, B cell lymphoma-2 (Bcl-2) and Bcl-2 associated X protein, respectively. The results of the present study suggested that CSCs may be a novel therapeutic target for the prevention of tumor relapse.
journal_name
Exp Ther Medjournal_title
Experimental and therapeutic medicineauthors
Wang Y,Teng JSdoi
10.3892/etm.2016.3303subject
Has Abstractpub_date
2016-07-01 00:00:00pages
81-86issue
1eissn
1792-0981issn
1792-1015pii
ETM-0-0-3303journal_volume
12pub_type
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
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journal_title:Experimental and therapeutic medicine
pub_type: 杂志文章
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