Abstract:
BACKGROUND/AIMS:De novo expression of CD44 is considered as a marker of parietal epithelial cell (PEC) activation. The aim of our study was to explore CD74 expression, which can form a complex with CD44, in PECs during the progression of focal segmental glomerulosclerosis (FSGS). To clarify the role of CD74 expression and of its interaction with CD44, we generated a new mouse model with enhanced PEC activation through lipopolysaccharide (LPS) application to adriamycin (ADR)-induced nephropathy mice (LPS-treated ADR mice). METHODS:As a new model, LPS was intraperitoneally injected into the mice 3 weeks after ADR injection. The mice were divided into 3 categories: control mice, ADR mice and LPS-treated ADR mice. Renal function parameters, histologic changes and immunohistochemical expression of CD74 and other PEC activation markers were analyzed after LPS application. RESULTS:After LPS stimulation, the glomeruli were characterized by enlarged epithelial cells with strong CD74 expression, followed by pseudo-crescent formation. By double staining, CD74-positive enlarged cells showed co-expression of classical PEC markers, but not of Lotus tetragonolobus lectin (marker of proximal tubular cells), suggesting amplification of PEC activation. Time-course analysis displayed marked upregulation of CD74 expression during rapid PEC activation compared with CD44. Additionally, the time-dependent change in ERK phosphorylation showed a similar pattern to CD74. CONCLUSION:Our results indicate that CD74 can be a marker for PEC activation in FSGS. By modifying the ADR mouse model through LPS treatment, we found that CD74 upregulation better reflects a rapid amplification of PEC activation than CD44 expression.
journal_name
Nephronjournal_title
Nephronauthors
Yamazaki T,Sasaki S,Okamoto T,Sato Y,Hayashi A,Ariga Tdoi
10.1159/000448221subject
Has Abstractpub_date
2016-01-01 00:00:00pages
238-252issue
4eissn
1660-8151issn
2235-3186pii
000448221journal_volume
134pub_type
杂志文章相关文献
NEPHRON文献大全abstract::Previous studies of IgA nephropathy have demonstrated a number of prognostically significant clinical and pathological factors in groups of patients with the full histological spectrum of the disease. Whether these factors can be applied to a group of IgA nephropathy patients with disease of moderate degree is unknown...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000184518
更新日期:1987-01-01 00:00:00
abstract::Animal models are essential tools to understand the mechanisms underlying the development and progression of renal disease and to study potential therapeutic approaches. Recently, interventional models suitable to induce acute and chronic kidney disease in the mouse have become a focus of interest due to the wide avai...
journal_title:Nephron
pub_type: 杂志文章,评审
doi:10.1159/000445171
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:The introduction of automated biopsy devices and the localization of the kidney by ultrasound were aimed at optimizing efficacy and safety of the percutaneous renal biopsy procedure. We evaluated these technological advances in our renal biopsies performed in children. METHODS:We sequentially used the Silve...
journal_title:Nephron
pub_type: 临床试验,杂志文章
doi:10.1159/000045090
更新日期:1998-08-01 00:00:00
abstract::Mitochondria contain electron-dense particles, partly composed of an amorphous form of calcium phosphate. We have used electron microscopy from percutaneous renal biopsy material to analyze mitochondrial granulation in the proximal renal tubule of nonuremic and uremic children. Based on a technique of cutting mitochon...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000181739
更新日期:1979-01-01 00:00:00
abstract::Secondary hyperparathyroidism is common in chronic renal failure and is due to inadequate synthesis of calcitriol, the active metabolite of vitamin D. Intravenous administration of alphacalcidol, a synthetic analogue which is metabolized to calcitriol, was given during 12 weeks to 51 patients on chronic hemodialysis i...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000186004
更新日期:1990-01-01 00:00:00
abstract::The renal cortex of anesthetized dogs was found to contain per gram lyophilized dry weight 9.5+/-0.6 micronmol ATP, 3.2+/-0.5 MICRONMOL ADP, 0.68+/-0.3 MICRONMOL AMP, and 22.5+/-4.4 micronmol Pi. These amounts were unchanged by the administration of moderate amounts of saline or hypertonic mannitol in load doses of ab...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000180833
更新日期:1977-01-01 00:00:00
abstract::3-Deoxyglucosone (3-DG) has been identified as an intermediate of the Maillard reaction in vitro. We measured serum 3-DG levels using gas chromatography/mass spectrometry and found a marked elevation in serum 3-DG levels in uremic patients compared with healthy subjects. The uremic patients with diabetes showed signif...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000188516
更新日期:1995-01-01 00:00:00
abstract::Blood eosinophils were counted by the counting-chamber method in 21 regular hemodialysis patients. Before dialysis, eosinophil counts were within the normal range in all patients (mean 98 cells/mm3, range 6-350). After the first 15 min of hemodialysis, with not reused coils or hollow-fiber dialysers, a significant dro...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000182805
更新日期:1982-01-01 00:00:00
abstract::29 patients receiving haemodialysis treatment for chronic renal failure were divided into two groups on the basis of the presence or absence of bone disease as defined by radiology and bone alkaline phosphatase. The group of patient with bone disease showed a significantly greater increase in protein-bound calcium dur...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000181632
更新日期:1979-01-01 00:00:00
abstract::Ischemic acute kidney injury (AKI) is predominantly mediated by the innate inflammatory response to damage-associated molecular patterns released during the reperfusion phase of the ischemic event. In this study, we show that pre-emptive IgM infusions to increase binding of natural IgM (nIgM) anti-leucocyte autoantibo...
journal_title:Nephron
pub_type: 杂志文章,评审
doi:10.1159/000501639
更新日期:2019-01-01 00:00:00
abstract::Although serologic studies have identified hantaviral infection in the United States, acute disease has not been recognized. This study describes 3 cases of domestically acquired hemorrhagic fever with renal syndrome (HFRS) in the United States. Infection was due to a local strain of Seoul virus (Baltimore rat virus)....
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000188086
更新日期:1994-01-01 00:00:00
abstract::Sickle cell nephropathy characterized by proteinuria and predominantly glomerular lesions has not been studied as extensively as renal tubular alterations in sickle cell disease. We reviewed our experience with this entity over a 14-year period. Of 13 children with either proteinuria or the nephrotic syndrome, 8 showe...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000183404
更新日期:1985-01-01 00:00:00
abstract::The study aimed to evaluate the behavior of alpha-glutathione S-transferase (alpha-GST) in the serum of hemodialysis patients with hepatitis C virus (HCV) infection following treatment with high-dose IFN-alpha-2b. Ten patients with detected anti-HCV antibodies and HCV RNA by RT-PCR were selected and treated with high-...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000045363
更新日期:1999-01-01 00:00:00
abstract:BACKGROUND:The prevalence of subclinical atherosclerosis and the main predictors of progression of this condition in patients undergoing peritoneal dialysis (PD) have been insufficiently investigated. OBJECTIVES AND METHOD:Following a prospective, multicenter, observational design, we studied 237 patients who were tre...
journal_title:Nephron
pub_type: 杂志文章,多中心研究
doi:10.1159/000458418
更新日期:2017-01-01 00:00:00
abstract::The role of T cell-mediated immunity in the early phase of nephrotoxic serum nephritis (NTN) was examined by transfer of pan T, CD8-positive, and CD4-positive cells. The disease was induced by transferring rabbit gamma-globulin (RGG)-sensitized T cells to nude (rnu/rnu) rats pretreated with a subnephritogenic dose (a ...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000188400
更新日期:1994-01-01 00:00:00
abstract::It is unknown whether continuous renal replacement techniques result in diminished morbidity and mortality when compared to conventional dialytic techniques. To investigate this issue a previously described, retrospectively studied group of critically ill patients with severe acute renal failure treated by conventiona...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000188675
更新日期:1995-01-01 00:00:00
abstract::This study was undertaken to test the hypothesis that ischemia prior to transplantation causes tubular damage without clinical evidence of graft dysfunction. The urinary excretion of fructose-1,6-bisphosphatase (EC 3.1.3.11, FBPase), a cytosolic enzyme located exclusively in the proximal tubules, and the lysosomal enz...
journal_title:Nephron
pub_type: 临床试验,杂志文章
doi:10.1159/000190248
更新日期:1997-01-01 00:00:00
abstract::During hemodialysis, platelets are activated across a dialyzer. Soluble P-selectin (sP-selectin) is a form of P-selectin which is a glycoprotein relocated from secretory granules to the surfaces of platelets and endothelial cells after these cells have been physiologically activated. To investigate whether sP-selectin...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000044903
更新日期:1998-01-01 00:00:00
abstract::The number of mast cells in the skin was evaluated in 25 patients with end-stage renal failure on different treatment modality (conservative, hemodialysis and peritoneal dialysis). According to the presence of pruritus, uremic patients were divided into two groups: group A, 13 patients with diffuse pruritus, and group...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000186826
更新日期:1992-01-01 00:00:00
abstract::Urinary excretion of oxalate, calcium and urate has been investigated in 88 patients affected by idiopathic calcium oxalate stone disease and in 20 normal subjects. Of these ions, only oxalate was found significantly higher in stone formers. Defining hyperoxaluria as urinary oxalate excretion greater than 2 SD above n...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000183037
更新日期:1983-01-01 00:00:00
abstract::The 24-hour urinary excretion of kallikrein (K) and prostaglandin E2 (PGE2), which reflects their intrarenal synthesis, was measured in 7 normal women (NW), 10 women with essential hypertension (EH), 26 normal pregnant women (NP), 12 women with hypertension in pregnancy (HP), and 4 women with toxemia. All pregnant wom...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000183426
更新日期:1985-01-01 00:00:00
abstract::An unusual case of progressive systemic sclerosis with rapidly progressing renal failure after a successful delivery is described. The presenting syndrome was malignant hypertension. The blood pressure was refractory to the various therapeutic measures which were instituted, including hemodialysis. The patient, theref...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000180812
更新日期:1977-01-01 00:00:00
abstract::Serum leptin concentrations in normal humans have been reported to correlate with the body mass index (BMI) as well as with the body fat mass. In this study, we measured serum leptin concentrations in 107 patients on hemodialysis, 30 of whom had diabetes mellitus as the cause, and examined the clinical significance. F...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000045122
更新日期:1998-09-01 00:00:00
abstract:BACKGROUND:Home hemodialysis is usually considered a superior therapy, whose decline is related to demographic, social, psychological and financial factors as well as to competition with renal transplantation and PD. METHODS:A home hemodialysis program was started in November 1998 in the University of Torino, Italy (2...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000063319
更新日期:2002-10-01 00:00:00
abstract::Clearance techniques were used to evaluate renal tubular sodium and water excretion in 4 patients with antibiotic-induced acute renal failure (ARF). Creatinine clearances and maximal urine flow rates of patients with ARF (22.6 and 5.23 ml/min, respectively) were significantly lower than control values during hypotonic...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000181226
更新日期:1978-01-01 00:00:00
abstract::Renal scarring is considered to be a characteristic of reflux nephropathy. The effects of ulinastatin, a strong inhibitor of polymorphonuclear leukocyte elastase, on renal scarring following direct parenchymal or intravesical ascending infection by Serratia marcescens or Escherichia coli were determined. Four days of ...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000188362
更新日期:1995-01-01 00:00:00
abstract::We investigated changes in the levels of mRNA transcripts encoding types I and IV collagen in the kidney following the administration of ciclosporin (CS) in mice. Daily doses of CS increased the levels of mRNAs encoding collagen types I and IV in whole kidneys harvested 4 weeks after treatment. At this time point, nei...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000186710
更新日期:1992-01-01 00:00:00
abstract:INTRODUCTION:In an attempt to decrease mortality in patients with end-stage renal disease, an increase in the lifetime of these patients without much focus on health-related quality of life (HRQOL) was pursued for a long period of time. However, lately, an improvement in the quality of this extended lifetime has focuse...
journal_title:Nephron
pub_type: 杂志文章,多中心研究
doi:10.1159/000369139
更新日期:2015-01-01 00:00:00
abstract::Efficacy and safety of a low-molecular-weight heparin (LMWH) were studied in 33 stable maintenance hemodialysis patients who had a bleeding tendency on unfractionated heparin. The optimal dose of LMWH for each patient was titrated before the study; the mean total LMWH dosage was 1,152 +/- 574 IU. No major bleeding or ...
journal_title:Nephron
pub_type: 临床试验,杂志文章
doi:10.1159/000045840
更新日期:2000-12-01 00:00:00
abstract::Experiences with peritonitis in a continuous ambulatory peritoneal dialysis (CAPD) program at a single center over 8 years were reviewed. Home-acquired peritonitis rates have been less than 1 episode per patient year since 1982. Gram-positive organisms continue to account for most episodes in a similar proportion. Act...
journal_title:Nephron
pub_type: 杂志文章
doi:10.1159/000183807
更新日期:1986-01-01 00:00:00