Abstract:
:This study describes the development and cell culture application of nanometer thick photocrosslinkable thermoresponsive polymer films prepared by physical adsorption. Two thermoresponsive polymers, poly(N-isopropylacrylamide (NIPAm)-co-acrylamidebenzophenone (AcBzPh)) and poly(NIPAm-co-AcBzPh-co-N-tertbutylacrylamide) are investigated. Films are prepared both above and below the polymers' lower critical solution temperatures (LCSTs) and cross-linked, to determine the effect, adsorption preparation temperature has on the resultant film. The films prepared at temperatures below the LCST are smoother, thinner, and more hydrophilic than those prepared above. Human pulmonary microvascular endothelial cell (HPMEC) adhesion and proliferation are superior on the films produced below the polymers LCST compared to those produced above. Cells sheets are detached by simply lowering the ambient temperature to below the LCST. Transmission electron, scanning electron, and light microscopies indicate that the detached HPMEC sheets maintain their integrity.
journal_name
Macromol Bioscijournal_title
Macromolecular bioscienceauthors
Healy D,Nash ME,Gorelov A,Thompson K,Dockery P,Beloshapkin S,Rochev Ydoi
10.1002/mabi.201600175subject
Has Abstractpub_date
2017-02-01 00:00:00issue
2eissn
1616-5187issn
1616-5195journal_volume
17pub_type
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