Abstract：
:Various types of microfluidic systems have been developed to detect bacterial pathogens. However, most of these require enrichment steps that take at least several hours when detecting bacteria that are present with a low number of cells and, in addition, fabrication requires complicated assembly steps. In this study, we report the development of 3D microfluidic magnetic preconcentrator (3DμFMP) made of plastic via 3D printing without the need for any assembly. 3DμFMP could selectively preconcentrate enterohemorrhagic Escherichia coli O157:H7 in 100mL by a factor of 700 within 1h using antibody-conjugated magnetic nanoparticles (Ab-MNPs). With the combined use of an ATP luminometer, as low as 10 E. coli O157:H7 CFU (colony forming unit)/mL could be detected in blood. These results demonstrate the feasibility of 3DμFMP as a preconcentrator to improve the detection limit of existing bacterial detection systems.
journal_name
J Microbiol Methodsjournal_title
Journal of microbiological methodsauthors
Park C,Lee J,Kim Y,Kim J,Lee J,Park Sdoi
10.1016/j.mimet.2016.12.001subject
Has Abstractpub_date
2017-01-01 00:00:00pages
128-133eissn
0167-7012issn
1872-8359pii
S0167-7012(16)30344-Xjournal_volume
132pub_type
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