Abstract：
:The aim of this study was to investigate the gene structure of two hormone-sensitive lipase b (HSLb) isoforms and their transcriptional regulation by insulin in grass carp (Ctenopharyngodon idella). HSL is an important lipolytic enzyme responsible for the hydrolysis of triacylglycerols (TAGs). Two isoforms (HSLa and HSLb) have been cloned in fish, but information about their gene structure and function is very few. In this study, a novel grass carp HSLb isoform (HSLb2) were firstly isolated and characterized from grass carp, encoding peptides of 848 amino acid residues. HSLb2 comprises 13 coding exons and contains a different exon encoding six amino acids in the 5'-region compared to previous reported HSLb (HSLb1), revealing that alternative multiple exons usage (Exon b1 and Exon b2) results in a significant variation in the 5'-region of HSLb transcripts. Exon b2 is located close to the 3' end of exon b1. Both HSLb2 and HSLb1 mRNAs were expressed in a wide range of tissues, but the abundance of each HSLb messenger RNA (mRNA) showed the tissue-dependent expression patterns. Incubation of hepatocytes with insulin in vitro reduced the mRNA levels of HSLb2 rather than HSLb1, suggesting two HSLb forms may serve somewhat different roles in the regulation of lipogenesis by insulin. To our knowledge, for the first time, the present study provides evidence that HSLb1 and HSLb2 are differentially expressed among tissues and also differentially regulated by insulin in vitro, which provide the groundwork to elucidate the gene structure and physiological function of HSL in fish.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Sun J,Yang Z,Xiao P,Liu Y,Ji H,Du Z,Chen Ldoi
10.1007/s10695-016-0308-1subject
Has Abstractpub_date
2017-04-01 00:00:00pages
539-547issue
2eissn
0920-1742issn
1573-5168pii
10.1007/s10695-016-0308-1journal_volume
43pub_type
杂志文章abstract：:Selected biochemical parameters were measured in the plasma of both underyearling anadromous steelhead trout (Salmo gairdneri) and underyearling residentS. gairdneri. The analyses were conducted in an effort to determine whether or not there might be changes which could be associated with the parr-smolt transformation...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01874727
更新日期：1988-03-01 00:00:00
abstract：:Three cytosolic glutathione S-transferases [E.C. 2.5.1.18] were identified in liver of a marine flatfish, the plaice (Pleuronectes platessa) and the two major isoforms were purified to homogeneity. There was no evidence for the presence of basic transferases. The plaice GST's exhibited comparable hematin binding to ra...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00003400
更新日期：1990-11-01 00:00:00
abstract：:The stress response transmitted by the HPA axis is one of the best examples of neuroendocrine-immune interactions that are critical for survival. Analogous to the situation in mammals, the stress response in fish is characterized by the activation of the hypothalamo-pituitary-interrenal axis (HPI). Effects of cortisol...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0084-3
更新日期：2015-10-01 00:00:00
abstract：:Connective tissue growth factor (ctgf) is involved in the proliferation, migration, adhesion of cell, and the constituent of extracellular matrix, which plays an important role in embryogenesis, angiogenesis, wound repair, and fibrosis diseases. In this study, the cDNA sequence of grass carp ctgf gene was cloned by ra...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00653-2
更新日期：2019-08-01 00:00:00
abstract：:In vertebrate species, the biopotential embryonic gonad differentiation is affected by many key genes and key steroidogenic enzymes. Insulin-like growth factor-1 receptor (Igf1r) has been considered as an important sex-differentiation gene in mammals and could mediate the biological action of Igf1, an important regula...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-014-9914-y
更新日期：2014-08-01 00:00:00
abstract：:Dietary fatty acids, particularly arachidonic acid (ARA), affect cortisol and may influence the expression of genes involved in stress response in fish. The involvement of ARA on stress, lipid, and eicosanoid metabolism genes, in Senegalese sole, was tested. Post-larvae were fed Artemia presenting graded ARA levels (0...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-013-9778-6
更新日期：2013-10-01 00:00:00
abstract：:In vitro studies have suggested that connexin43 (cx43) expression is of particular importance during establishment and regeneration of the mammalian hematopoietic system. However, little is known about its in vivo functions during hematopoiesis due to the embryonic lethality of mammalian knockout models. In this study...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-009-9371-1
更新日期：2010-12-01 00:00:00
abstract：:The glucose-regulated protein, 94 kDa (GRP94), is an endoplasmic reticulum (ER)-localized heat shock protein that plays among other functions a crucial role in folding and exports of Toll-like receptors (TLRs) and some other immune-relevant factors. We identified two copies of the GRP94-encoding gene in rainbow trout ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-014-9979-7
更新日期：2014-12-01 00:00:00
abstract：:Scrutiny of the zebrafish (Danio rerio) genomic database confirmed eight functional vitellogenin (vtg) genes, each with one or two transcript variants, and the encoded Vtg polypeptides were structurally and functionally characterized in detail by in silico and experimental analyses. There were five type I (vtgs1, 4, 5...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-018-0524-y
更新日期：2018-12-01 00:00:00
abstract：:The composition of seminal plasma and metabolism of sperm of the cyprinid fishAlburnus alburnus were investigated. Statistically significant correlations were found between motility parameters and seminal fluid osmolality, pH, Na(+), K(+) and protein levels (negative correlations: % immotile spermatozoa-Na(+), K(+); p...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01875596
更新日期：1996-04-01 00:00:00
abstract：:Environmental stressors caused by inadequate aquaculture management strategies suppress the immune response of fish and make them more susceptible to diseases. Therefore, efforts have been made to relieve stress in fish by using various functional feed additives in the diet, including probiotics. The present work eval...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-016-0277-4
更新日期：2017-02-01 00:00:00
abstract：:The pineal organ of fish, through its 24h rhythmic release of melatonin, acts as a transducer of the photoperiod, influencing different physiological functions (e.g., reproduction, growth). The target sites for melatonin are poorly known in fish, especially marine species. A radioligand study was undertaken using the ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01875583
更新日期：1996-11-01 00:00:00
abstract：:Prolactin (PRL) is one of the most versatile hormones found in the pituitary of vertebrates and exerts its actions through binding to a specific PRL receptor (PRLR). Here we describe the cloning and characterization of a second prolactin receptor (ntPRLR2), isolated from the ovary of Nile tilapia (Oreochromis niloticu...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-009-9355-1
更新日期：2010-06-01 00:00:00
abstract：:Muscle accretion is affected by the difference between protein synthesis and its degradation. Studies on different species revealed that muscle proteolysis is mediated by different pathways including the ubiquitin-proteasome pathway in which the ubiquitin protein ligases play an important role. These muscle atrophy as...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00667-w
更新日期：2019-08-01 00:00:00
abstract：:Plasma amino acid profiles in the systemic circulation of rainbow trout acclimated to diets containing different protein sources, with and without supplementation with free amino acids, were monitored for up to 120 hours after consumption of the diets. Plasma concentrations of lysine, methionine, and isoleucine increa...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004309
更新日期：1995-06-01 00:00:00
abstract：:The aim of this study was to use more accurate techniques to investigate the effects of cryoprotectants (CPAs) and extenders on post-thaw sperm quality of Brycon orbignyanus and Prochilodus lineatus. Six freezing media comprising the combination of three CPAs (DMSO, methanol and methyl glycol) and two extenders (BTS a...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-014-0016-7
更新日期：2015-02-01 00:00:00
abstract：:Calcium depletion/replacement studies were carried out to examine the role of calcium in contraction of trout intestinal smoot muscle in vitro. Three chemically distinct calcium channel blockers were used to determine whether voltage operated calcium channels (VOCs) were involved in calcium entry with either agonist o...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00003410
更新日期：1990-11-01 00:00:00
abstract：:In fish, as in mammals, several studies have demonstrated that the cocaine- and amphetamine-regulated transcript (CART) plays an important role in feeding. However, thus far, the function of CART in gibel carp (Carassius auratus gibelio) feeding regulation has not been reported. In our study, we first identified three...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-018-0596-8
更新日期：2019-06-01 00:00:00
abstract：:Endocrine-disrupting chemicals (EDCs) can affect normal sexual differentiation in fish. Foxl2, one forkhead transcription factor, plays an important role in ovarian differentiation in the early development of the female gonad in mammals and fish. How EDCs affect Foxl2 expression is little known. In this study, we isol...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9548-2
更新日期：2012-06-01 00:00:00
abstract：:Rhamdia quelen morphophysiological responses to propofol sedation were examined. The purpose was to investigate whether propofol would be a suitable drug to be used in fish transport procedures. Fish were exposed to 0, 0.4 or 0.8 mg L(-1) propofol for 1, 6 or 12 h in 40 L tanks, simulating open transport systems. Prop...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-014-9997-5
更新日期：2015-04-01 00:00:00
abstract：:In aquaculture, antibiotics are the traditional treatment used against bacterial infections. However, their use has increasingly come into question given their effects on fish and, possibly, on human health. Consequently, there is interest in developing alternative treatments aimed at stimulating the innate immune res...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0155-5
更新日期：2016-04-01 00:00:00
abstract：:An ovarian cell line was successfully developed from the juvenile ovary of Southern catfish (SCO1) (Silurus meridionalis), which was designated as SCO1. The cell line multiplied preferentially in L-15 medium with 15 % fetal bovine serum at 28 °C for more than 70 passages over a period of 420 days. SCO1 showed fibrobla...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-014-9932-9
更新日期：2014-10-01 00:00:00
abstract：:Concentration, ability to motility, motility during the second activation (reactivation), and endogenous respiration were studied in sperm from two experimental groups of carp males. Group 1 was maintained for 7 days at 15 degrees C (cold water (CW) group), whereas the second group was subjected to a temperature of 20...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-008-9292-4
更新日期：2009-11-01 00:00:00
abstract：:In this study, the mechanism that VC inhibits lipid deposition through GSK-3β/mTOR signaling was investigated in the liver of Danio rerio. The results indicated that 0.5- and 1.0-g/kg VC treatments activated mTOR signaling by inhibiting GSK-3β expression. The mRNA expression of FAS, ACC, and ACL, as well as the conten...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00727-1
更新日期：2020-02-01 00:00:00
abstract：:This study was conducted to determine the effects of dietary supplementation of lemon balm (Melissa officinalis) aqueous methanolic extract on growth performance, blood parameters, digestive and antioxidant enzyme activities, and non-specific immune responses in rainbow trout (Oncorhynchus mykiss). Fish with an averag...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00737-z
更新日期：2020-02-01 00:00:00
abstract：:A 90-day randomized feeding experiment was performed to assess the effects of dietary cobalt (Co) supplementation on the growth performance, muscle composition, status of iron and manganese in the muscle as well as the expression of growth-related genes in the muscle (myoblast determination protein 1 homolog (MyoD) an...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00723-5
更新日期：2020-02-01 00:00:00
abstract：:The incorporation and metabolism of (n-3) and (n-6) polyunsaturated fatty acids (PUFA) supplemented to growing cultures were studied in Atlantic salmon (AS) cells. A fatty acid concentration of 25 μM considerably altered the fatty acid composition of AS cells without increasing the neutral lipid content of the cells o...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00003426
更新日期：1990-07-01 00:00:00
abstract：:The antioxidative role of cerium was investigated in the liver of silver crucian carp injected with lead. The fish were intraperitoneally injected with 10, 20, or 30 mg/kg wet weight PbCl(2). After a 14-day period of incubation, 35 animals were injected with a solution of 1.5 mg/kg wet weight CeCl(3). After 42 days, t...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-008-9301-7
更新日期：2010-09-01 00:00:00
abstract：:The effects of paraquat (PQ; 1,1'-dimethyl-4,4'-bipyridylium dichloride) treatment were investigated in carp, silver carp and wels. The serum glutamic-oxalacetic transaminase (GOT; L-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.1.1.) level was enhanced by 50% at 1 ppm exposure and by 100% at 10 ppm exposure in a...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004434
更新日期：1990-01-01 00:00:00
abstract：:Juvenile sea bass, 1.5 years old, of mixed sex, held on long photoperiods were fed early on the photoperiod and late on the photoperiod, using different diets. Fish fed natural diets showed a daily rhythmicity of plasma insulin, liver c-AMP, plasma glucose, liver glycogen and muscle glycogen content, however, fish fed...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01874796
更新日期：1988-10-01 00:00:00
