Abstract:
:Dietary fatty acids, particularly arachidonic acid (ARA), affect cortisol and may influence the expression of genes involved in stress response in fish. The involvement of ARA on stress, lipid, and eicosanoid metabolism genes, in Senegalese sole, was tested. Post-larvae were fed Artemia presenting graded ARA levels (0.1, 0.4, 0.8, 1.7, and 2.3%, dry matter basis), from 22 to 35 days after hatch. Whole-body cortisol levels were determined, before and 3 h after a 2 min air exposure, as well as the expression of phospholipase A2 (PLA 2 ), cyclooxygenase-2 (COX-2), steroidogenic acute regulatory protein (StAR), glucocorticoid receptors (GRs), phosphoenolpyruvate carboxykinase (PEPCK), and peroxisome proliferator-activated receptor alpha (PPARα). Relative growth rate (6.0-7.8% day(-1)) and survival at the end of the experiment (91-96%) and after stress (100%) were unaffected. Fish reflected dietary ARA content and post-stress cortisol increased with ARA supply up to 1.7%, whereas 2.3% ARA seemed to enhance basal cortisol slightly and alter the response to stress. Results suggested that elevating StAR transcription might not be necessary for a short-term response to acute stress. Basal cortisol and PLA 2 expression were strongly correlated, indicating a potential role for this enzyme in steroidogenesis. Under basal conditions, larval ARA was associated with GR1 expression, whereas the glucocorticoid responsive gene PEPCK was strongly related with cortisol but not GR1 mRNA levels, suggesting the latter might not reflect the amount of GR1 protein in sole. Furthermore, a possible role for PPARα in the expression of PEPCK following acute stress is proposed.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Martins DA,Rocha F,Castanheira F,Mendes A,Pousão-Ferreira P,Bandarra N,Coutinho J,Morais S,Yúfera M,Conceição LE,Martínez-Rodríguez Gdoi
10.1007/s10695-013-9778-6subject
Has Abstractpub_date
2013-10-01 00:00:00pages
1223-38issue
5eissn
0920-1742issn
1573-5168journal_volume
39pub_type
杂志文章abstract::Iron is one the most abundant metals on the earth being essential for living organisms even though its free form can be toxic. The overload of this metal may be related with some disorders, like Alzheimer and Parkinson diseases, and hemochromatosis in the liver. The aim of the present study was to evaluate the effects...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-010-9459-7
更新日期:2011-09-01 00:00:00
abstract::A study was conducted to investigate the effect of dietary yeast polysaccharides on some hematologic parameters and intestinal morphology of channel catfish. Channel catfish were fed diets containing yeast polysaccharides at 0 (control), 0.1, 0.2, or 0.3 % for 7 weeks. Each diet was provided to 10 channel catfish spec...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10695-012-9631-3
更新日期:2012-10-01 00:00:00
abstract::Transferrin mRNA in liver was quantified in farmed Atlantic salmon (Salmo salar) fed three different levels (310-, 65-, and 40 ppm) of dietary iron for 26 weeks. At the time of sampling, the fish fed 40 ppm iron showed symptoms of iron deficiency. Mean values of liver storage iron was reduced from 84 to 12 mg kg(-1) a...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF02112358
更新日期:1996-08-01 00:00:00
abstract::Selected biochemical parameters were measured in the plasma of both underyearling anadromous steelhead trout (Salmo gairdneri) and underyearling residentS. gairdneri. The analyses were conducted in an effort to determine whether or not there might be changes which could be associated with the parr-smolt transformation...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01874727
更新日期:1988-03-01 00:00:00
abstract::The large yellow croaker (Larimichthys crocea) is a marine fish that is economically important to Chinese fisheries, and its reproductive and developmental biology have been extensively investigated. However, the molecular mechanism of oogenesis in L. crocea is not clear. Here, we investigated the multiple vitellogeni...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-018-0569-y
更新日期:2019-06-01 00:00:00
abstract::Studies on the digestive secretions in aquatic animals can elucidate certain aspects of their nutritive physiology. The aim of the present study was to compare the digestive lipase and phospholipase activities in ten marine species belonging to four classes following the taxonomic classification of marine organisms. A...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-012-9633-1
更新日期:2012-10-01 00:00:00
abstract::Evidence has recently been presented for variation in the inducibility of various 7-alkoxycoumarin-O-dealkylase activities in liver microsomes from a number of mammalian species by β-naphthoflavone (βNF). In the present study we have investigated the inducibility of hepatic microsomal 7-methoxycoumarin-O-demethylase, ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01875022
更新日期:1989-07-01 00:00:00
abstract::A study on the effect of three different anabolic-androgenic steroids on the growth, food conversion efficiency and nucleic acid contents of liver, kidney, brain and muscle of carp,Cyprinus carpio was undertaken. The three steroids, methyltestosterone (MT), ethylestrenol (EE) and oxandrolone (ON), were fed in differen...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01874771
更新日期:1989-05-01 00:00:00
abstract::The composition of seminal plasma and metabolism of sperm of the cyprinid fishAlburnus alburnus were investigated. Statistically significant correlations were found between motility parameters and seminal fluid osmolality, pH, Na(+), K(+) and protein levels (negative correlations: % immotile spermatozoa-Na(+), K(+); p...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01875596
更新日期:1996-04-01 00:00:00
abstract::Cytochrome P450 aromatase, which is encoded by the CYP19a gene, converts androgens to estradiol. Considerable evidence suggests that estrogens play an important role in fish reproductive process. Therefore CYP19a is an excellent candidate gene for reproductive traits. Variants in the promoter of the CYP19a gene might ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-008-9237-y
更新日期:2009-08-01 00:00:00
abstract::The oxidative status is associated with animal lifespan, metabolism, activity and circadian rhythms. The objective of this work is to study the time course of the oxidative status over a daily cycle in the plasma, liver and brain, and the changes in the plasma cortisol levels of sturgeon and trout. The knowledge of da...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-017-0356-1
更新日期:2017-08-01 00:00:00
abstract::In most sciaenids, males possess sonic muscles and produce sound through the contraction of these muscles and amplification of the swim bladder. The sonic muscles in some fishes exhibit seasonal changes in size. For example, they are hypertrophic in the spawning season, and atrophic in the non-spawning months. The pro...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-011-9502-3
更新日期:2011-12-01 00:00:00
abstract::To determine if there were organ-specific changes in immune responses or immune-endocrine interaction, we monitored in vitro immune response, cortisol sensitivity and number and affinity of glucocorticoid receptors (GR) in leukocytes from freshwater-adapted juvenile coho salmon (Oncorhynchus kisutch) during the physio...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004605
更新日期:1993-04-01 00:00:00
abstract::This investigation was performed to monitor hexachlorocyclohexane isomers (HCHs), dichlorodiphenyltrichloroethane (DDT, and its metabolites, refered to as DDTs), plasma levels of estradiol-17beta (E2), and the gonadosomatic index (GSI) between sampling sites of unpolluted ponds of Gujartal, Jaunpur (control site) and ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-007-9142-9
更新日期:2008-03-01 00:00:00
abstract::Vitellogenin (VTG) from spotted wolffish, Anarhichas minor, a candidate species for cold-water marine aquaculture, was purified by MgCl₂/EDTA precipitation followed by a two-step chromatographic procedure. VTG had an apparent molecular mass of 470 kDa, as determined by gel filtration, and an amino acid composition sim...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-013-9843-1
更新日期:2014-02-01 00:00:00
abstract::The activities of trypsin (EC 3.4.21.4), chymotrypsin (EC 3.4.21.1), lipase (EC 3.1.1.3) and amylase (EC 3.2.1.1) were measured in different regions of the alimentary tract of ammocoetes from each of the three extant lamprey families. In the southern hemisphere speciesGeotria australis (Geotriidae), and even more part...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004649
更新日期:1992-05-01 00:00:00
abstract::We have studied the location and the ontogeny of the digestive enzyme, phospholipase A2 (PLA2) immunohistochemically in the adult and larvae/juvenile of the red sea breamPagrus major by using an antiserum against theNaja naja venom PLA2. The antiserum reacts with at least one enzyme among the PLA2s purified from the f...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF02274224
更新日期:1992-02-01 00:00:00
abstract::The aim of this study was to investigate the expression patterns of ocular melatonin in the annual reproductive cycle of the female grass puffer. Spawning season of the female grass puffer is from June to July in Jeju, South Korea. Time-resolved fluoroimmunoassay revealed that levels of ocular melatonin, which show an...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00749-9
更新日期:2020-06-01 00:00:00
abstract::The present study was conducted to investigate the effects of high dietary lipid levels on growth, metabolism, antioxidant capacity, and immune responses of largemouth bass. Fish (initial body weight 13.38 ± 0.11 g) were fed three isonitrogenous semi-purified diets containing 5%, 10%, and 20% lipid, respectively. The ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00705-7
更新日期:2020-02-01 00:00:00
abstract::The proline-rich protein 13 (PRR13) is reported to be a key regulator of the resistance to cytostatica by decreasing the copy number of the proapoptotic gene thrombospondin-1. We isolated and characterized the complete PRR13 gene sequence of rainbow trout (Oncorhynchus mykiss). The gene comprises four exons and three ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-010-9407-6
更新日期:2010-12-01 00:00:00
abstract::High-mobility group box 2 (HMGB2) is a nonhistone architectural protein that plays important roles in many biological processes. In this study, we cloned a homologue of the HMGB2 from the lymphocyte-like cells of Lampetra japonica (L. japonica). Sequence analysis reveals that L. japonica HMGB2 contains two highly cons...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-013-9871-x
更新日期:2014-04-01 00:00:00
abstract::Changes in serum thyroxine (T4), estradiol-17β (E2) and testosterone (T) levels during the parr-smolt transformation (smoltification) were investigated in the masu salmon (Oncorhynchus masou) in 1985 and 1987. T4 showed a peak in levels at the early stage of smoltification and E2 and T levels peaked almost at the same...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004317
更新日期:1993-07-01 00:00:00
abstract::Immunoglobulin M (IgM) is known as a main factor in the humoral immune system of teleosts. In the present study, the effect of cortisol on plasma IgM concentrations was investigated using a specific antibody to IgM in masu salmon (Oncorhynchus masou). Cortisol was orally administered each day for 2 weeks at a dose of ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004118
更新日期:1994-05-01 00:00:00
abstract::Connective tissue growth factor (ctgf) is involved in the proliferation, migration, adhesion of cell, and the constituent of extracellular matrix, which plays an important role in embryogenesis, angiogenesis, wound repair, and fibrosis diseases. In this study, the cDNA sequence of grass carp ctgf gene was cloned by ra...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00653-2
更新日期:2019-08-01 00:00:00
abstract::The incorporation and metabolism of (n-3) and (n-6) polyunsaturated fatty acids (PUFA) supplemented to growing cultures were studied in Atlantic salmon (AS) cells. A fatty acid concentration of 25 μM considerably altered the fatty acid composition of AS cells without increasing the neutral lipid content of the cells o...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00003426
更新日期:1990-07-01 00:00:00
abstract::The aims of the present study were to characterize sperm quality and to quantify seasonal changes in sexual hormone (testosterone [T], 11-ketotestosterone [11-KT] and 17,20β-dihydroxypregn-4-en-3-one [17,20β-P]) levels in male brill (Scophthalmus rhombus) plasma, as well as to test a more intensive sampling strategy t...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-012-9692-3
更新日期:2013-04-01 00:00:00
abstract::Histo- and immunohistochemical techniques have recently been used to study the fibre type and myosin expression in fish muscle during development. In the present work, embryonic, larval and adult myosin isozymes (heavy and light chains) and parvalbumin isotypes were analyzed, from fertization to the adult stage, by po...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004524
更新日期:1992-08-01 00:00:00
abstract::CDK-2, a member of the cyclin-dependent kinase family, plays an important role in many cell processes, such as cell cycle regulation, cell growth and differentiation, and cell apoptosis. Lampreys belong to the most primitive vertebrates, and there is no report about the CDK-2 gene in lampreys at present. In this study...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00683-w
更新日期:2019-12-01 00:00:00
abstract::The activities of several digestive enzymes during larval development of the spotted sand bass (Paralabrax maculatofasciatus) were evaluated using electrophoretic techniques. The results show the presence of three isoforms of alkaline protease from day 2 after hatching (ah) and the early appearance of one pepsin-like ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-008-9276-4
更新日期:2010-03-01 00:00:00
abstract::Red crucian carp (Carassius auratus red var.) is an ornamental fish with vivid red/orange color. It has been found that the adult body color of this strain forms a gray-to-red change. In this study, skin transcriptomes of red crucian carp are first obtained for three different stages of body color development, named b...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-017-0379-7
更新日期:2017-10-01 00:00:00