Abstract：
:Histo- and immunohistochemical techniques have recently been used to study the fibre type and myosin expression in fish muscle during development. In the present work, embryonic, larval and adult myosin isozymes (heavy and light chains) and parvalbumin isotypes were analyzed, from fertization to the adult stage, by polyacrylamide gel electrophoresis of barbel (Barbus barbus L.) trunk muscle extracts. The examined myosins display the sequential transitions from embryonic to larval and adult forms characteristic of higher vertebrates. They are characterized by specific heavy chains but their light chains differ only by the LC1/LC3 stoichiometry with LC3 exceeding LC1 after 10 days. Sarcoplasmic parvalbumins show considerable and unforeseen developmental transitions in their isotype distribution: the PA II isotype first appears after hatching and becomes the predominant form until the length reaches about 6 cm. One month after hatching, the amount of PA II then decreases and the synthesis of PA III and IV further increases to reach the typical adult pattern at a size of 18 cm. These observations show that the distribution of parvalbumin isotypes reflects the stage of development. It suggests a specific role for each isotype in relation to muscle activity. Microscopy illustrates the progressive development of somites, muscles cells, and myofibrils, which accelerates at hatching when movements increase.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Focant B,Huriaux F,Vandewalle P,Castelli M,Goessens Gdoi
10.1007/BF00004524subject
Has Abstractpub_date
1992-08-01 00:00:00pages
133-43issue
2eissn
0920-1742issn
1573-5168journal_volume
10pub_type
杂志文章abstract：:The questions addressed in this study were: 1) whether insulin added to the incubation medium can down-regulate (125)I insulin binding to isolated hepatocytes of Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss); 2) whether quantitative assessment of insulin processing can be made on isolated fish ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004590
更新日期：1993-07-01 00:00:00
abstract：:Bacillus circulans PB7, isolated from the intestine of Catla catla, was evaluated for use as a probiotic supplement in the feeds for the fingerlings of Catla catla. The effect of supplement on the growth performance, feed utilization efficiency, and immune response was evaluated. Catla fingerlings (ave. wt. 6.48 +/- 0...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-008-9272-8
更新日期：2009-08-01 00:00:00
abstract：:Plasma chemistry, lipid metabolism and vitellogenin gene expression of captive Sterlet sturgeon Acipenser ruthenus were studied in different maturity stages. A total of 32 fish were sampled, and maturity stages were identified on the basis of histological criteria and direct observation. Females were classified to fou...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0189-8
更新日期：2016-06-01 00:00:00
abstract：:Red crucian carp (Carassius auratus red var.) is an ornamental fish with vivid red/orange color. It has been found that the adult body color of this strain forms a gray-to-red change. In this study, skin transcriptomes of red crucian carp are first obtained for three different stages of body color development, named b...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-017-0379-7
更新日期：2017-10-01 00:00:00
abstract：:Levels of two types of gonadotropin-releasing hormone (salmon GnRH and chicken GnRH-II) in the brain and pituitary, and content of gonadotropin (GTHIβ and IIβ) in the pituitary were measured in male masu salmon from hatching to gonadal maturation for three years in order to clarify the involvement of GnRHs in precocio...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004570
更新日期：1993-07-01 00:00:00
abstract：:Changes in tissue and plasma isoenzymes of alkaline phosphatase (ALP) were qualitatively and quantitatively determined for male and female Atlantic salmon parr, silvery parr, smolt, immature grilse, prespawning grilse and postspawning grilse using cellulose acetate electrophoresis, densitometry and spectrophotometry. ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004451
更新日期：1994-03-01 00:00:00
abstract：:In aquaculture, fish species may experience stressful episodes caused by poor farming conditions. The exponential increase of global aquaculture has raised the number of research studies aimed at demonstrating the sensitivity of aquatic animals in confined environments. The development of a real-time PCR and immunohis...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-020-00811-x
更新日期：2020-08-01 00:00:00
abstract：:Three consecutive days of injections of triiodothyronine (T3)(0.038, 0.075, 0.15 and 1.54 nmoles/g) significantly elevated the acetylcholinesterase (AchE) activity in the brain of Singi fish, Heteropneustes fossilis (Bloch). The higher doses of 0.075, 0.15 and 1.54 nmoles of T3/g induced a greater increase in enzyme a...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004508
更新日期：1993-03-01 00:00:00
abstract：:The analysis of the tench growth hormone gene structure revealed a comparable organization of coding and non-coding regions than other from cyprinid species. Based on the performed mRNA and amino acid sequence alignments, gh tench is related to Asian than to European representatives of Cyprinidae family. Second aim of...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-012-9661-x
更新日期：2012-12-01 00:00:00
abstract：:An embryonic stem (ES)-like cell culture system RESC from a commercially important freshwater carp, Labeo rohita, was developed using blastula stage embryos. The cells were cultured in Leibovitz-15 (L-15) medium in gelatin-coated cell culture flask supplemented with 15 % fetal bovine serum along with 10 ng ml(-1) basi...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-012-9674-5
更新日期：2012-12-01 00:00:00
abstract：:A 60-day feeding trial was conducted to study the effect of exogenous enzymes (xylanase and phytase) supplementation in the non-fermented and fermented de-oiled rice bran (DORB)-based diet of Labeo rohita. Four test diets (T1-DORB-based diet, T2-fermented DORB-based diet, T3-phytase and xylanase supplemented DORB-base...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-018-0492-2
更新日期：2018-08-01 00:00:00
abstract：:The present study explored the protective role of dietary the extract of Angelica sinensis (EAs) on high density, CuSO4, or trichlorfon-treated Crucian carp (Carassius auratus auratus). Firstly, the study showed that the optimum density for growth and growth inhibition was 0.49 and 0.98 fish L-1 water, respectively. D...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-020-00821-9
更新日期：2020-10-01 00:00:00
abstract：:Fish suffer from anemia and hypovolemic hypotensive shock after in vivo exposure with microcystins.However, except for in vivo causes for anemia and hypotension, an in vitro study of fish erythrocytes exposed to MC is necessary. For a better understanding of hematology toxicity of MC, the main aim of the present study...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9572-2
更新日期：2012-06-01 00:00:00
abstract：:The present study develops an experimental procedure aimed to estimate the efficiency of protein digestion in fish by measuring both gut transit rate and total amount of the main intestinal proteases (trypsin and chymotrypsin). The selected species was the Nile tilapia (Oreochromis niloticus). Total time for digestion...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-010-9385-8
更新日期：2010-12-01 00:00:00
abstract：:Aryl hydrocarbon receptor (AhR) agonists are known to cause lethal cardiovascular deformities in fish after developmental exposure. Acute adult fish toxicity of AhR agonists is thought to be minimal, but limited evidence suggests sublethal effects may also involve the cardiac system in fish. In the present study, adul...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-014-9982-z
更新日期：2015-02-01 00:00:00
abstract：:Feeding experiments and laboratory analyses were conducted to establish the essential fatty acid (EFA) requirement of red drum (Sciaenops ocellatus). Juvenile red drum were maintained in aquaria containing brackish water (5 ± 2‰ total dissolved solids) for two 6-week experiments. Semipurified diets contained a total o...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004370
更新日期：1993-10-01 00:00:00
abstract：:The yellow catfish Pelteobagrus fulvidraco with initial average weight 16.6 ± 0.17 g were fed three extruded diets for 56 days. Fish meal (FM) diet was formulated as the normal control with 380 g FM and 200 g soybean meal (SBM) kg-1 diet. The SBM diet was prepared with 220 g FM and 360 g SBM kg-1 diet without Lys or M...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10695-017-0437-1
更新日期：2018-02-01 00:00:00
abstract：:Teleosts have many spawning strategies and the hormonal control of gametogenesis is not well defined among the species or even, between sexes. To increase the knowledge of gonadotropin hormones, we studied the trend by gene expression of gonadotropin receptors in the follicles and testis at different maturity stages i...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-018-0479-z
更新日期：2018-06-01 00:00:00
abstract：:The dependence of the effects of zinc and copper on the activities of proteinases of the stomach and intestinal mucosa on temperature and pH in four species of boreal piscivorous fish (pike Esox lucius, zander Zander lucioperca, perch Perca fluviatilis and burbot Lota lota) as well as in some of their potential preys ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-009-9353-3
更新日期：2010-09-01 00:00:00
abstract：:The development of digestive enzymes during the early ontogeny of the Mayan cichlid (Cichlasoma urophthalmus) was studied using biochemical and electrophoretic techniques. From yolk absorption (6 days after hatching: dah), larvae were fed Artemia nauplii until 15 dah, afterward they were fed with commercial microparti...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-010-9431-6
更新日期：2011-03-01 00:00:00
abstract：:The activities of several digestive enzymes during larval development of the spotted sand bass (Paralabrax maculatofasciatus) were evaluated using electrophoretic techniques. The results show the presence of three isoforms of alkaline protease from day 2 after hatching (ah) and the early appearance of one pepsin-like ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-008-9276-4
更新日期：2010-03-01 00:00:00
abstract：:In order to assess the digestive physiological capacity of the American shad Alosa sapidissima and to establish feeding protocols that match larval nutritional requirements, we investigated the ontogenesis of digestive enzymes (trypsin, amylase, lipase, pepsin, alkaline phosphatase, and leucine aminopeptidase) in larv...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-016-0295-2
更新日期：2017-04-01 00:00:00
abstract：:The main indication of cimetidine is being H₂-receptor antagonist, but studies suggest that cimetidine may also act as a non-specific stimulant of cell-mediated immunity and immunomodulator. In order to determine the immunomodulatory effect of dietary intake of cimetidine in the common carp (100 ± 10 g), subjects were...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-013-9804-8
更新日期：2013-12-01 00:00:00
abstract：:A study on the effect of three different anabolic-androgenic steroids on the growth, food conversion efficiency and nucleic acid contents of liver, kidney, brain and muscle of carp,Cyprinus carpio was undertaken. The three steroids, methyltestosterone (MT), ethylestrenol (EE) and oxandrolone (ON), were fed in differen...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01874771
更新日期：1989-05-01 00:00:00
abstract：:The biological activities of lamprey GnRH and analogs were determined in the adult male lamprey,Petromyzon marinus. Two successive injections of lamprey GnRH at 0.005, 0.065 or 0.163 μg/g body weight or [D-Ala(6),Pro(9)-NHEt] mammal GnRH at 0.05 μg/g stimulated plasma estradiol and progesterone levels in adult male la...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004695
更新日期：1989-06-01 00:00:00
abstract：:The plasma levels of estradiol-17β (E2), 17α, 20β-dihydroxy-4-pregnen-3-one (17,20-P) and gonadotropin (GTH) were measured in brook trout (Salvelinus fontinalis) during the period from the end of vitellogenesis to postovulation. Blood samples were taken according to specific stages of maturation, including germinal ve...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF02180281
更新日期：1987-06-01 00:00:00
abstract：:Three cytosolic glutathione S-transferases [E.C. 2.5.1.18] were identified in liver of a marine flatfish, the plaice (Pleuronectes platessa) and the two major isoforms were purified to homogeneity. There was no evidence for the presence of basic transferases. The plaice GST's exhibited comparable hematin binding to ra...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00003400
更新日期：1990-11-01 00:00:00
abstract：:This investigation was performed to monitor hexachlorocyclohexane isomers (HCHs), dichlorodiphenyltrichloroethane (DDT, and its metabolites, refered to as DDTs), plasma levels of estradiol-17beta (E2), and the gonadosomatic index (GSI) between sampling sites of unpolluted ponds of Gujartal, Jaunpur (control site) and ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-007-9142-9
更新日期：2008-03-01 00:00:00
abstract：:The pineal organ of fish, through its 24h rhythmic release of melatonin, acts as a transducer of the photoperiod, influencing different physiological functions (e.g., reproduction, growth). The target sites for melatonin are poorly known in fish, especially marine species. A radioligand study was undertaken using the ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01875583
更新日期：1996-11-01 00:00:00
abstract：:The aim of this study was to investigate the gene structure of two hormone-sensitive lipase b (HSLb) isoforms and their transcriptional regulation by insulin in grass carp (Ctenopharyngodon idella). HSL is an important lipolytic enzyme responsible for the hydrolysis of triacylglycerols (TAGs). Two isoforms (HSLa and H...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-016-0308-1
更新日期：2017-04-01 00:00:00
