Abstract:
:Three cytosolic glutathione S-transferases [E.C. 2.5.1.18] were identified in liver of a marine flatfish, the plaice (Pleuronectes platessa) and the two major isoforms were purified to homogeneity. There was no evidence for the presence of basic transferases. The plaice GST's exhibited comparable hematin binding to rat ligandin, however, bilirubin was only bound weakly and the enzymes did not display the inhibition characteristics of ligandin. They were extremely sensitive to inhibition by organotin compounds.Plaice transferase A was a homodimer of Mr 27 kDa subunits; it displayed strongest activity with CDNB and some activity with DCNB as substrates. Specific antibodies to it did not show any relationship with other plaice GST's or rat GST subunits 1,2,3,4,7,8 or 10. Plaice transferase B was a homodimer of Mr 25 kDa subunits, it displayed similar activities with CDNB and DCNB as substrates to transferase A and a low activity with ethacrynic acid or p-nitrobenzylchloride. It was immunologically related to the rat alpha class transferase subunits 1,2 and 8. A minor form, transferase AM, which was not separated from transferase A, appeared to be a heterodimer of Mr 25.5 and 27 kDa subunits. It displayed a greater activity with DCNB than the other plaice GST's and some activity with bromosulphalein indicative of a possible relationship with the Mu class transferases.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
George SG,Buchanan Gdoi
10.1007/BF00003400subject
Has Abstractpub_date
1990-11-01 00:00:00pages
437-49issue
6eissn
0920-1742issn
1573-5168journal_volume
8pub_type
杂志文章abstract::Muscle accretion is affected by the difference between protein synthesis and its degradation. Studies on different species revealed that muscle proteolysis is mediated by different pathways including the ubiquitin-proteasome pathway in which the ubiquitin protein ligases play an important role. These muscle atrophy as...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00667-w
更新日期:2019-08-01 00:00:00
abstract::Ubiquitin-conjugating enzymes (E2s) play an important role in the mechanism of ubiquitin transfer. Although in most species many of these enzymes share high sequence and structural conservation, their existence and functions in the lamprey remain unknown. In this study, we identified and characterized a ubiquitin-conj...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0139-5
更新日期:2016-02-01 00:00:00
abstract::The tilapia prolactin (PRL) cell responds rapidly (10-20 min) to small physiological changes in medium osmotic pressure (OP), releasing increasing quantities of hormone as medium OP is reduced. This release is rapidly (≤ 10 min) inhibited by somatostatin (SRIF). There is now extensive evidence that tilapia PRL cell fu...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004685
更新日期:1989-06-01 00:00:00
abstract::The stress response transmitted by the HPA axis is one of the best examples of neuroendocrine-immune interactions that are critical for survival. Analogous to the situation in mammals, the stress response in fish is characterized by the activation of the hypothalamo-pituitary-interrenal axis (HPI). Effects of cortisol...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0084-3
更新日期:2015-10-01 00:00:00
abstract::Spotted wolffish Anarhichas minor reproduction in captivity is dependent on in vitro fertilization. However, it is often challenging to acquire sufficient fresh sperm to fertilize the eggs that are obtained. In this study, we evaluate the possibility to store spotted wolffish sperm by refrigeration. Spotted wolffish s...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-020-00820-w
更新日期:2020-06-02 00:00:00
abstract::Immunoglobulin M (IgM) is known as a main factor in the humoral immune system of teleosts. In the present study, the effect of cortisol on plasma IgM concentrations was investigated using a specific antibody to IgM in masu salmon (Oncorhynchus masou). Cortisol was orally administered each day for 2 weeks at a dose of ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004118
更新日期:1994-05-01 00:00:00
abstract::Goldfish,Carassius auratus, were acclimated for 2 to 3.5 weeks to three temperature regimes: [1] temporally-constant (10, 20 and 30°C), [2] diurnally-cycling (20 ± 10°C) and [3] randomly-fluctuating (± 2°C at approximately 2h intervals between extremes of 10 and 30°C). No significant differences in hematocrit were evi...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004327
更新日期:1994-12-01 00:00:00
abstract::The present study aims to investigate the effects of both garlic and curcumin through evaluating their therapeutic properties as antioxidants on liver and kidney functions, hepatic antioxidants and GPx gene expression against aflatoxicosis of O. niloticus. In total, 180 of tilapia were divided into ten groups; T1 repr...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0164-4
更新日期:2016-04-01 00:00:00
abstract::Annual plasma insulin and glucose cycles were studied inDicentrarchus labrax maintained in either seawater (37.8‰) or brackish water (3.5‰). In both media, the highest insulin levels were found during the prespawning period (August-November) coincident to increases in weight and a decrease in plasma glucose. During sp...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF02110880
更新日期:1987-10-01 00:00:00
abstract::The study assessed the effects of surgically implanted dummy ultrasonic transmitters on physiological response of bighead carp Hypophthalmichthys nobilis in April 2011. Before the surgery, 15 blood samples were extracted randomly from 195 bighead carp samples, and then the rest of the fish were divided into three grou...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-014-9945-4
更新日期:2014-10-01 00:00:00
abstract::Connective tissue growth factor (ctgf) is involved in the proliferation, migration, adhesion of cell, and the constituent of extracellular matrix, which plays an important role in embryogenesis, angiogenesis, wound repair, and fibrosis diseases. In this study, the cDNA sequence of grass carp ctgf gene was cloned by ra...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00653-2
更新日期:2019-08-01 00:00:00
abstract::Copper is one of the major heavy metal pollutants found in the aquatic environment. Therefore, it is important for determining the genes that play a key role in copper metabolism in aquatic organisms. This study, thus, aimed to identify a new copper-inducible gene in swordtail fish, Xiphophorus helleri. Using ACP-base...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-007-9153-6
更新日期:2008-06-01 00:00:00
abstract::In the present study, different reference genes were isolated, and their stability in the skeletal muscle of fine flounder subjected to different nutritional states was assessed using geNorm and NormFinder. The combinations between 18S and ActB; Fau and 18S; and Fau and Tubb were chosen as the most stable gene combina...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-012-9739-5
更新日期:2013-08-01 00:00:00
abstract::Groupers are widely distributed throughout the tropical and subtropical waters of the world and are regarded as a favourite marine food fish. However, their large-scale aquaculture has been hindered by the rarity of natural males. Being protogynous hermaphrodites, groupers have been considered as study model for devel...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-008-9219-0
更新日期:2010-06-01 00:00:00
abstract::A 60-day feeding trial was conducted to study the effect of exogenous enzymes (xylanase and phytase) supplementation in the non-fermented and fermented de-oiled rice bran (DORB)-based diet of Labeo rohita. Four test diets (T1-DORB-based diet, T2-fermented DORB-based diet, T3-phytase and xylanase supplemented DORB-base...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-018-0492-2
更新日期:2018-08-01 00:00:00
abstract::We have studied the location and the ontogeny of the digestive enzyme, phospholipase A2 (PLA2) immunohistochemically in the adult and larvae/juvenile of the red sea breamPagrus major by using an antiserum against theNaja naja venom PLA2. The antiserum reacts with at least one enzyme among the PLA2s purified from the f...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF02274224
更新日期:1992-02-01 00:00:00
abstract::Vitellogenin (VTG), the egg yolk precursor protein, was purified from plasma of estradiol-3-benzoate (E2B)-treated male shorthead redhorse (Moxostoma macrolepidotum) and immature copper redhorse (Moxostoma hubbsi) by a two-step chromatographic procedure without precipitation. Intact VTGs appeared as dimers with appare...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-008-9205-6
更新日期:2009-06-01 00:00:00
abstract::Trypsin from the viscera of Bogue (Boops boops) was purified to homogeneity by precipitation with ammonium sulphate, Sephadex G-100 gel filtration and Mono Q-Sepharose anion exchange chromatography, with an 8.5-fold increase in specific activity and 36% recovery. The molecular weight of the purified enzyme was estimat...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-009-9365-z
更新日期:2010-12-01 00:00:00
abstract::The involvement of the freshwater fish gill chloride cells (CCs) in trans-branchial calcium uptake (JinCa(2+)) was investigated. This was accomplished by assessing the interspecific relationships between the apical surface area of CCs exposed to the external environment and JinCa(2+). Three species of freshwater teleo...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004482
更新日期:1992-12-01 00:00:00
abstract::The toxic effects of thyroxine (T4F), levothyroxine (L-T4), and thyroxine complexed into β-cyclodextrin (β-CD-T4) on the biological parameters of tambaqui (Colossoma macropomum) were evaluated. The animals were exposed to a chronic toxicity test based on concentrations of influent (60 ng/L) for 2 months. Weight, total...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00630-9
更新日期:2019-06-01 00:00:00
abstract::Muscle tissues contribute 34-48 % of the total body mass in fish. Proteomic analysis enables better understanding of the skeletal muscle physiology and metabolism. A proteome map reflects the general fingerprinting of the fish species and has the potential to identify novel proteins which could serve as biomarkers for...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0046-9
更新日期:2015-06-01 00:00:00
abstract::Gonadotropins (GTH) were isolated from pituitary glands of grass carp and their properties investigated. Acetone-dried pituitaries were extracted with ammonium acetate-ethanol and were then passed through cation exchanger, anion exchanger, and gel filtration column. Two glycoprotein gonadotropins (DE I and DE II) were...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004705
更新日期:1989-06-01 00:00:00
abstract::The pseudobranch in rainbow trout was investigated in order to clarify its metabolic and physiological function. Contents of lactate, glycogen, and glycerides and activities of carbonic anhydrase (CA), lactate dehydrogenase (LDH), and 3-hydroxy-acyl CoA dehydrogenase (HAD) were measured and compared with those in the ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004070
更新日期:1995-08-01 00:00:00
abstract::This study aimed to characterize the full-length cDNA of glucose-6-phosphate dehydrogenase (G6PD) from Megalobrama amblycephala with its responses to dietary carbohydrate levels characterized. The cDNA obtained covered 2768 bp with an open reading frame of 1572 bp. Sequence alignment and phylogenetic analysis revealed...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-018-0572-3
更新日期:2019-02-01 00:00:00
abstract::Mineral needs as affected by changes in dietary protein and oil sources were studied in rainbow trout. Duplicate groups (n = 30 fish per replicate) of rainbow trout (initial BW: 37 g) were fed either a fish meal/fish oil-based (M) or a complete plant ingredient (V)-based diet at four graded ration (R) levels [apparent...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-014-0020-y
更新日期:2015-02-01 00:00:00
abstract::Measurable in vitro hepatic monodeiodinase activity of the southern hemisphere lamprey, Geotria australis, was present only during the first 5 of the 16 month upstream spawning migration of this species. The production of T3 from T4 in vitro was pH-sensitive, and exhibited typical Michaelis-Menton kinetics. No consist...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00003372
更新日期:1990-09-01 00:00:00
abstract::In this study, a freshwater fish Channa punctatus was exposed to subacute concentrations of synthetic pyrethroid insecticides (cypermethrin and lambda-cyhalothrin) for 96 h to evaluate their impact on the levels of nucleic acids and protein in its different organs. Significant enhancement in the level of DNA was recor...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-007-9192-z
更新日期:2008-12-01 00:00:00
abstract::Monitoring the growth of salmon during their early marine phase provides insights into prey availability, and growth rates may be linked to risks of size-dependent mortality. However, the measurement of growth rate is challenging for free-living salmon in the ocean. Insulin-like growth factor (IGF)-I is a growth-promo...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00681-y
更新日期:2019-12-01 00:00:00
abstract::River damming and building of hydroelectric power plants interrupt the reproductive migration routes and change the major physicochemical parameters of water quality, with drastic consequences for populations of migratory fishes. The goal of this study was to evaluate proliferation and cell death during spermatogenesi...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-012-9668-3
更新日期:2012-12-01 00:00:00
abstract::Gilthead sea bream larvae (Sparus aurata) aged 47 days post hatching (dph) (11.6-12.8 mg in wet weight) were exposed to several sublethal concentrations of Cd(2+) (0.1, 5 and 10 mg/L) during 6 days in order to investigate the effects of this heavy metal on the expression of selected genes involved in detoxification (m...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-012-9727-9
更新日期:2013-06-01 00:00:00