Abstract:
:In vitro studies have suggested that connexin43 (cx43) expression is of particular importance during establishment and regeneration of the mammalian hematopoietic system. However, little is known about its in vivo functions during hematopoiesis due to the embryonic lethality of mammalian knockout models. In this study, we observed that zebrafish cx43 is not only expressed in the eyes, cerebellum, heart, and vasculature, but also expressed, albeit at low levels, in intermediate cell mass (ICM, the primitive hematopoietic site). Knockdown of cx43 leads to vacuolization in the wedge of the ICM and an apparent reduction in the number of circulating blood cells, but does not affect their cellular morphology. Whole-mount in situ hybridization analysis revealed that the hemangioblastic marker flk-1 and the primitive hematopoietic markers lmo2 and scl are basically maintained at normal levels in cx43 morphant embryos at 12-13 h postfertilization (hpf) compared with the con-MO injected embryos. However, subsequent expression of the definitive hematopoietic stem cell (HSC) marker c-myb was severely downregulated in the ventral wall of the dorsal aorta of cx43-depleted embryos at 36 hpf. Furthermore, we confirmed this phenotype by injection of cx43-MO into Tg(gata1:EGFP) embryos. Together, our results show that cx43 contributes to late primitive and definitive hematopoiesis in zebrafish embryos.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Jiang Q,Liu D,Sun S,Hu J,Tan L,Wang Y,Gui Y,Yu M,Song Hdoi
10.1007/s10695-009-9371-1subject
Has Abstractpub_date
2010-12-01 00:00:00pages
945-51issue
4eissn
0920-1742issn
1573-5168journal_volume
36pub_type
杂志文章abstract::Ubiquitin-conjugating enzymes (E2s) play an important role in the mechanism of ubiquitin transfer. Although in most species many of these enzymes share high sequence and structural conservation, their existence and functions in the lamprey remain unknown. In this study, we identified and characterized a ubiquitin-conj...
journal_title:Fish physiology and biochemistry
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journal_title:Fish physiology and biochemistry
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journal_title:Fish physiology and biochemistry
pub_type: 杂志文章,随机对照试验
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abstract::The pseudobranch in rainbow trout was investigated in order to clarify its metabolic and physiological function. Contents of lactate, glycogen, and glycerides and activities of carbonic anhydrase (CA), lactate dehydrogenase (LDH), and 3-hydroxy-acyl CoA dehydrogenase (HAD) were measured and compared with those in the ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004070
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abstract::To determine if there were organ-specific changes in immune responses or immune-endocrine interaction, we monitored in vitro immune response, cortisol sensitivity and number and affinity of glucocorticoid receptors (GR) in leukocytes from freshwater-adapted juvenile coho salmon (Oncorhynchus kisutch) during the physio...
journal_title:Fish physiology and biochemistry
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journal_title:Fish physiology and biochemistry
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journal_title:Fish physiology and biochemistry
pub_type: 杂志文章,随机对照试验
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更新日期:2018-02-01 00:00:00
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更新日期:2008-12-01 00:00:00
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journal_title:Fish physiology and biochemistry
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更新日期:2015-04-01 00:00:00
abstract::In fish, as in mammals, several studies have demonstrated that the cocaine- and amphetamine-regulated transcript (CART) plays an important role in feeding. However, thus far, the function of CART in gibel carp (Carassius auratus gibelio) feeding regulation has not been reported. In our study, we first identified three...
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journal_title:Fish physiology and biochemistry
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更新日期:2008-03-01 00:00:00
abstract::Plasma chemistry, lipid metabolism and vitellogenin gene expression of captive Sterlet sturgeon Acipenser ruthenus were studied in different maturity stages. A total of 32 fish were sampled, and maturity stages were identified on the basis of histological criteria and direct observation. Females were classified to fou...
journal_title:Fish physiology and biochemistry
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journal_title:Fish physiology and biochemistry
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journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004550
更新日期:1993-07-01 00:00:00
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journal_title:Fish physiology and biochemistry
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更新日期:2020-02-01 00:00:00
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journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
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journal_title:Fish physiology and biochemistry
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journal_title:Fish physiology and biochemistry
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journal_title:Fish physiology and biochemistry
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journal_title:Fish physiology and biochemistry
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更新日期:2011-06-01 00:00:00
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journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
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更新日期:2008-12-01 00:00:00
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journal_title:Fish physiology and biochemistry
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