Abstract:
:Muscle accretion is affected by the difference between protein synthesis and its degradation. Studies on different species revealed that muscle proteolysis is mediated by different pathways including the ubiquitin-proteasome pathway in which the ubiquitin protein ligases play an important role. These muscle atrophy associated ligases were not well studied in tilapia. In this study, we characterized the ubiquitin protein ligases MuRF1/2/3, Atrogin-1 and F-box25, members of the ubiquitin-proteasome pathway in tilapia, Oreochromis niloticus, and their expressions in the muscle of starved, fed, refed, and control fish. Sequences of these genes revealed presence of Ring finger, B-box, and Cos domains in all MuRF genes, as well as F-box domain in Atrogin-1 and F-box25 genes. Real-time qPCR data analysis showed that expression of MuRF1/2/3, Atrogin-1, F-box25, and proteasome complex genes was significantly upregulated in starved fish compared to fed fish. Concurrently, the proteasome activity was 1.7-folds elevated in the starved fish compared to fed fish. These results confirm the important role of these genes in muscle degradation and suggest potential usage as markers of muscle accretion in tilapia.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Shaalan WM,El-Hameid NAA,El-Serafy SS,Salem Mdoi
10.1007/s10695-019-00667-wsubject
Has Abstractpub_date
2019-08-01 00:00:00pages
1321-1330issue
4eissn
0920-1742issn
1573-5168pii
10.1007/s10695-019-00667-wjournal_volume
45pub_type
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