Vitamin C inhibits lipid deposition through GSK-3β/mTOR signaling in the liver of zebrafish.

Abstract:

:In this study, the mechanism that VC inhibits lipid deposition through GSK-3β/mTOR signaling was investigated in the liver of Danio rerio. The results indicated that 0.5- and 1.0-g/kg VC treatments activated mTOR signaling by inhibiting GSK-3β expression. The mRNA expression of FAS, ACC, and ACL, as well as the content of TG, TC, and NEFA, was decreased by 0.5- and 1.0-g/kg VC treatments. Moreover, to confirm GSK-3β playing a key role in regulating TSC2 and mTOR, GSK-3β RNA was interfered and the activity of GSK-3β was inhibited by 25- and 50-mg/L LiCl treatments, respectively. The results indicated that GSK-3β inactivation played a significant role in inducing mTOR signaling and inhibiting lipid deposition. VC treatments could induce mTOR signaling by inhibiting GSK-3β, and mTOR further participated in regulating lipid deposition by controlling lipid profile in the liver of zebrafish.

journal_name

Fish Physiol Biochem

authors

Liu D,Gu Y,Pang Q,Han Q,Li A,Wu W,Zhang X,Shi Q,Zhu L,Yu H,Zhang Q

doi

10.1007/s10695-019-00727-1

subject

Has Abstract

pub_date

2020-02-01 00:00:00

pages

383-394

issue

1

eissn

0920-1742

issn

1573-5168

pii

10.1007/s10695-019-00727-1

journal_volume

46

pub_type

杂志文章