Abstract:
:In this study, the mechanism that VC inhibits lipid deposition through GSK-3β/mTOR signaling was investigated in the liver of Danio rerio. The results indicated that 0.5- and 1.0-g/kg VC treatments activated mTOR signaling by inhibiting GSK-3β expression. The mRNA expression of FAS, ACC, and ACL, as well as the content of TG, TC, and NEFA, was decreased by 0.5- and 1.0-g/kg VC treatments. Moreover, to confirm GSK-3β playing a key role in regulating TSC2 and mTOR, GSK-3β RNA was interfered and the activity of GSK-3β was inhibited by 25- and 50-mg/L LiCl treatments, respectively. The results indicated that GSK-3β inactivation played a significant role in inducing mTOR signaling and inhibiting lipid deposition. VC treatments could induce mTOR signaling by inhibiting GSK-3β, and mTOR further participated in regulating lipid deposition by controlling lipid profile in the liver of zebrafish.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Liu D,Gu Y,Pang Q,Han Q,Li A,Wu W,Zhang X,Shi Q,Zhu L,Yu H,Zhang Qdoi
10.1007/s10695-019-00727-1subject
Has Abstractpub_date
2020-02-01 00:00:00pages
383-394issue
1eissn
0920-1742issn
1573-5168pii
10.1007/s10695-019-00727-1journal_volume
46pub_type
杂志文章abstract::The incorporation and metabolism of (n-3) and (n-6) polyunsaturated fatty acids (PUFA) supplemented to growing cultures were studied in Atlantic salmon (AS) cells. A fatty acid concentration of 25 μM considerably altered the fatty acid composition of AS cells without increasing the neutral lipid content of the cells o...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00003426
更新日期:1990-07-01 00:00:00
abstract::Post mortem storage is a necessary process for removal of pin bones without destruction of fillets, thereby avoiding volume and economic loss. However, the enzymes involved in loosening pin bones during storage have not been studied to a great extent. In this study, the activities and localization of MMPs in the conne...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-016-0264-9
更新日期:2017-02-01 00:00:00
abstract::Fish suffer from anemia and hypovolemic hypotensive shock after in vivo exposure with microcystins.However, except for in vivo causes for anemia and hypotension, an in vitro study of fish erythrocytes exposed to MC is necessary. For a better understanding of hematology toxicity of MC, the main aim of the present study...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-011-9572-2
更新日期:2012-06-01 00:00:00
abstract::The study assessed the effects of surgically implanted dummy ultrasonic transmitters on physiological response of bighead carp Hypophthalmichthys nobilis in April 2011. Before the surgery, 15 blood samples were extracted randomly from 195 bighead carp samples, and then the rest of the fish were divided into three grou...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-014-9945-4
更新日期:2014-10-01 00:00:00
abstract::The present study was conducted to investigate the effects of high dietary lipid levels on growth, metabolism, antioxidant capacity, and immune responses of largemouth bass. Fish (initial body weight 13.38 ± 0.11 g) were fed three isonitrogenous semi-purified diets containing 5%, 10%, and 20% lipid, respectively. The ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00705-7
更新日期:2020-02-01 00:00:00
abstract::Ubiquitin-conjugating enzymes (E2s) play an important role in the mechanism of ubiquitin transfer. Although in most species many of these enzymes share high sequence and structural conservation, their existence and functions in the lamprey remain unknown. In this study, we identified and characterized a ubiquitin-conj...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0139-5
更新日期:2016-02-01 00:00:00
abstract::The tissue distribution pattern of lipid is highly diverse among different fish species. Tiger puffer has a special lipid storage pattern, storing lipid predominantly in liver. In order to better understand the lipid physiology in fish storing lipid in liver, the present study preliminarily investigated the tissue dis...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-020-00815-7
更新日期:2020-08-01 00:00:00
abstract::The intracellular handing of a mannose-terminated glycoprotein taken up in rainbow trout liver cells by receptor-mediated endocytosis has been studied. The intracellular transport and degradation of ovalbumin (OA) were studied by means of subcellular fractionation in Nycodenz gradients and by differential centrifugati...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF02265127
更新日期:1991-04-01 00:00:00
abstract::Body color variation has long been a hot research topic in evolutionary and functional biology. Oujiang color common carp (Cyprinus carpio var. color) is a well-known economical and ornamental fish. Three main types of pigments and four distinct color patterns are typical characters of Oujiang color common carp, which...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-018-0551-8
更新日期:2019-02-01 00:00:00
abstract::The biological actions of growth hormone (GH) are pleiotropic, including growth promotion, energy mobilization, gonadal development, appetite, and social behavior. The regulatory network for GH is complex and includes many central and peripheral endocrine factors as well as that from the environment. It is known that ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0074-5
更新日期:2015-10-01 00:00:00
abstract::Vitamin E, a potent antioxidant consisting of four isomers each (α, β, γ, δ) of tocopherol (T) and tocotrienol (T3), is found naturally in plant oils at different concentrations. In this study, four semi-purified isonitrogenous and isolipidic (10 %) diets containing canola oil, cold-pressed soybean oil, wheat germ oil...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-013-9799-1
更新日期:2013-12-01 00:00:00
abstract::Cells from a relatively stenohaline marine species, turbot (Scophthalmus maximus) (TF) and an anadromous species, Atlantic salmon (AS) were cultured in media supplemented with NaCl to produce OPs varying from 300 to 500 mOsm kg(-1) and the direct effects of OP (salinity) on the fatty acid compositions of the main glyc...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00002456
更新日期:1995-04-01 00:00:00
abstract::In the present study, different reference genes were isolated, and their stability in the skeletal muscle of fine flounder subjected to different nutritional states was assessed using geNorm and NormFinder. The combinations between 18S and ActB; Fau and 18S; and Fau and Tubb were chosen as the most stable gene combina...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-012-9739-5
更新日期:2013-08-01 00:00:00
abstract::The effects of acclimation temperature (15, 20, 25 °C) on routine oxygen consumption and post-exercise maximal oxygen consumption rates (MO2) were measured in juvenile shortnose sturgeon (Acipenser brevirostrum LeSueur, 1818). The routine MO2 of shortnose sturgeon increased significantly from 126.75 mg O2 h-1 kg-1 at ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-017-0368-x
更新日期:2017-10-01 00:00:00
abstract::Virgin female rainbow trout, Oncorhynchus mykiss, were unilaterally ovariectomised at various stages of ovarian development to investigate the effect of the removal of one ovary on subsequent recruitment and growth of follicles in the remaining ovary. The right ovary was removed from groups of 12-15 fish, 12, 7 and 4 ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00003435
更新日期:1994-10-01 00:00:00
abstract::The effects of paraquat (PQ; 1,1'-dimethyl-4,4'-bipyridylium dichloride) treatment were investigated in carp, silver carp and wels. The serum glutamic-oxalacetic transaminase (GOT; L-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.1.1.) level was enhanced by 50% at 1 ppm exposure and by 100% at 10 ppm exposure in a...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004434
更新日期:1990-01-01 00:00:00
abstract::Drinking in freshwater juvenile salmon was investigated in response to vasodilation by sodium nitroprusside (SNP), a nitric oxide donor, which significantly increased blood vessel diameter in Atlantic salmon alevins. Atlantic salmon fry (1-3 g), as previously shown, drank at a significant rate in fresh water which dou...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01874839
更新日期:1996-02-01 00:00:00
abstract::The objective of this study was to investigate the effects of difloxacin (DIF) and avermectin (AVM) on glutamate decarboxylase (GAD) and GABA-transaminase (GABA-T) in different tissues of crucian carp (Carassius auratus gibelio). After the treatments of DIF and AVM, the mRNA expressions of GAD and GABA-T in different ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-020-00847-z
更新日期:2020-12-01 00:00:00
abstract::River damming and building of hydroelectric power plants interrupt the reproductive migration routes and change the major physicochemical parameters of water quality, with drastic consequences for populations of migratory fishes. The goal of this study was to evaluate proliferation and cell death during spermatogenesi...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-012-9668-3
更新日期:2012-12-01 00:00:00
abstract::Levels of two types of gonadotropin-releasing hormone (salmon GnRH and chicken GnRH-II) in the brain and pituitary, and content of gonadotropin (GTHIβ and IIβ) in the pituitary were measured in male masu salmon from hatching to gonadal maturation for three years in order to clarify the involvement of GnRHs in precocio...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004570
更新日期:1993-07-01 00:00:00
abstract::Teleosts have many spawning strategies and the hormonal control of gametogenesis is not well defined among the species or even, between sexes. To increase the knowledge of gonadotropin hormones, we studied the trend by gene expression of gonadotropin receptors in the follicles and testis at different maturity stages i...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-018-0479-z
更新日期:2018-06-01 00:00:00
abstract::The regulation of the interrenal of teleostean fishes is reviewed from the perspective of non-classical control mechanisms and new evidence is presented suggesting gonadotropic control of the interrenal. Cortisol secretion by the interrenal, in addition to regulation by ACTH, appears to be mediated by other hormones. ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004715
更新日期:1989-06-01 00:00:00
abstract::This study compared Amur sturgeon (Acipenser schrenckii) and Chinese sturgeon (Acipenser sinensis) on several biochemical parameters including glucose (GLU), urea, lactate dehydrogenase (LDH-L), activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), direct bilirubin (D.BILI), alkaline phosphat...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-006-7134-9
更新日期:2006-03-01 00:00:00
abstract::Goldfish,Carassius auratus, were acclimated for 2 to 3.5 weeks to three temperature regimes: [1] temporally-constant (10, 20 and 30°C), [2] diurnally-cycling (20 ± 10°C) and [3] randomly-fluctuating (± 2°C at approximately 2h intervals between extremes of 10 and 30°C). No significant differences in hematocrit were evi...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004327
更新日期:1994-12-01 00:00:00
abstract::The growth of cyanobacteria (blue-green algae) is a typical phenomenon in water bodies worldwide. The use of silver carp (Hypophthalmichthys molitrix) to reduce excessive phytoplankton development is controversial. In the case of cyanobacteria, many of which are toxic, understanding their possible digestion mechanism ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00751-1
更新日期:2020-04-01 00:00:00
abstract::Calcium depletion/replacement studies were carried out to examine the role of calcium in contraction of trout intestinal smoot muscle in vitro. Three chemically distinct calcium channel blockers were used to determine whether voltage operated calcium channels (VOCs) were involved in calcium entry with either agonist o...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00003410
更新日期:1990-11-01 00:00:00
abstract::A study on the effect of three different anabolic-androgenic steroids on the growth, food conversion efficiency and nucleic acid contents of liver, kidney, brain and muscle of carp,Cyprinus carpio was undertaken. The three steroids, methyltestosterone (MT), ethylestrenol (EE) and oxandrolone (ON), were fed in differen...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01874771
更新日期:1989-05-01 00:00:00
abstract::In fish, as in mammals, several studies have demonstrated that the cocaine- and amphetamine-regulated transcript (CART) plays an important role in feeding. However, thus far, the function of CART in gibel carp (Carassius auratus gibelio) feeding regulation has not been reported. In our study, we first identified three...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-018-0596-8
更新日期:2019-06-01 00:00:00
abstract::Plasma and liver composition, liver enzyme activities, and metabolite flux in isolated hepatocytes have been studied in immature American eels,Anguilla rostrata, injected daily IP with saline or cortisol (0.35 mg/kg eel). Plasma cortisol values were significantly increased above saline controls in those eels receiving...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF02290211
更新日期:1986-05-01 00:00:00
abstract::In the catfish Heteropneustes fossilis, the anterior kidney is a hemopoietic tissue which surrounds the adrenal homologues, interrenal (IR) and chromaffin tissues corresponding to the adrenal cortical and adrenal medulla of higher mammals. The IR tissue is arranged in cell cords around the posterior cardinal vein (PCV...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-016-0218-2
更新日期:2016-10-01 00:00:00