Abstract:
:Ubiquitin-conjugating enzymes (E2s) play an important role in the mechanism of ubiquitin transfer. Although in most species many of these enzymes share high sequence and structural conservation, their existence and functions in the lamprey remain unknown. In this study, we identified and characterized a ubiquitin-conjugating enzyme (UBE2A)-like gene in lamprey. The gene, designated as LaUBE2A, contained a 456-bp open reading frame encoding a 152-amino acid protein with a typical UBC domain. Real-time PCR assay showed that LaUBE2A was expressed in various tissues of the adult lamprey, with higher levels in the leukocytes and muscle and lower levels in the skin and liver. The high conservation in amino acid sequence between LaUBE2A and UBE2As from Homo sapiens, Mus musculus, Cavia porcellus, and Alligator sinensi implied that the function of LaUBE2A may be similar to that of UBE2A.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Chen L,Wu F,Feng Bdoi
10.1007/s10695-015-0139-5subject
Has Abstractpub_date
2016-02-01 00:00:00pages
313-20issue
1eissn
0920-1742issn
1573-5168pii
10.1007/s10695-015-0139-5journal_volume
42pub_type
杂志文章abstract::Two-dimensional gel electrophoresis (2-DE), matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) mass spectrometry, and database searching were used to analyze the effects of triploidization heat shock treatment on protein expression in rainbow trout eyed embryo and fry. After fertilizatio...
journal_title:Fish physiology and biochemistry
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doi:10.1007/s10695-016-0312-5
更新日期:2017-04-01 00:00:00
abstract::Gonadotropins (GTH) were isolated from pituitary glands of grass carp and their properties investigated. Acetone-dried pituitaries were extracted with ammonium acetate-ethanol and were then passed through cation exchanger, anion exchanger, and gel filtration column. Two glycoprotein gonadotropins (DE I and DE II) were...
journal_title:Fish physiology and biochemistry
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journal_title:Fish physiology and biochemistry
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journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01871745
更新日期:1988-01-01 00:00:00
abstract::The involvement of the freshwater fish gill chloride cells (CCs) in trans-branchial calcium uptake (JinCa(2+)) was investigated. This was accomplished by assessing the interspecific relationships between the apical surface area of CCs exposed to the external environment and JinCa(2+). Three species of freshwater teleo...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004482
更新日期:1992-12-01 00:00:00
abstract::Fish metabolic allostatic dynamics, when animal present physiological modifications that can be strategies to survive, are important for promoting changes to ensure whole body self-protection and survival in chronic states of stress. To determine the impact of sequential stressors on pacu (Piaractus mesopotamicus), fi...
journal_title:Fish physiology and biochemistry
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更新日期:2020-08-01 00:00:00
abstract::Glutathione (GSH) concentration, the activity of its metabolizing enzymes, glutathione transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR), and the antioxidant enzyme catalase (CAT) in O. niloticus ovary and testis were examined. GSH concentration of O. niloticus testis exhibited high concentra...
journal_title:Fish physiology and biochemistry
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更新日期:2016-02-01 00:00:00
abstract::A completely randomized experimental design carried out to investigate the effects of different levels of Pediococcus acidilactici (PA) including 0 (basal diet as a control diet), 1 × 106, 2 × 106, 4 × 106, and 8 × 106 colony-forming unit (CFU) per gram of the diet for 60 days on the mucosal immunity responses, growth...
journal_title:Fish physiology and biochemistry
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abstract::The effects of different environmental salinities (0, 12, 40, and 55 ppt) on pepsinogen 2 (pga2), trypsinogen 2 (try2), chymotrypsinogen (ctr), and pancreatic alpha-amylase (amy2a) gene expression, and on the total activities of their corresponding enzymes, were assessed in Chelon labrosus juveniles, after their corre...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章,评审
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更新日期:2018-02-01 00:00:00
abstract::An immunohistochemical study of the sturgeon (Acipenser baeri) pituitary was undertaken using antisera directed against hormones from various classes of vertebrates, including the only pituitary hormone available from sturgeon, gonadotrophin. A positive reaction was obtained after application of antisera towards the f...
journal_title:Fish physiology and biochemistry
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更新日期:1988-07-01 00:00:00
abstract::Spotted wolffish Anarhichas minor reproduction in captivity is dependent on in vitro fertilization. However, it is often challenging to acquire sufficient fresh sperm to fertilize the eggs that are obtained. In this study, we evaluate the possibility to store spotted wolffish sperm by refrigeration. Spotted wolffish s...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-020-00820-w
更新日期:2020-06-02 00:00:00
abstract::Teleosts have many spawning strategies and the hormonal control of gametogenesis is not well defined among the species or even, between sexes. To increase the knowledge of gonadotropin hormones, we studied the trend by gene expression of gonadotropin receptors in the follicles and testis at different maturity stages i...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-018-0479-z
更新日期:2018-06-01 00:00:00
abstract::In mammals, androgens appear to enhance the development of primary ovarian follicles, but PI3K (phosphoinositide 3-kinases) pathway is well recognized as one of the critical pathways in early follicular development. Roles of the PI3K were revealed by deletion of PTEN (phosphatase and tensin homolog on chromosome 10). ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-011-9560-6
更新日期:2012-06-01 00:00:00
abstract::The extensive use of pharmaceuticals in human and veterinary medicine may enter the aquatic environment and pose a serious threat to non-target aquatic organisms like fish. In this study, Indian major carp Cirrhinus mrigala was exposed to different concentrations (1, 10 and 100 μg L⁻¹) of most commonly used pharmaceut...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-013-9797-3
更新日期:2013-12-01 00:00:00
abstract::Changes in serum thyroxine (T4), estradiol-17β (E2) and testosterone (T) levels during the parr-smolt transformation (smoltification) were investigated in the masu salmon (Oncorhynchus masou) in 1985 and 1987. T4 showed a peak in levels at the early stage of smoltification and E2 and T levels peaked almost at the same...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004317
更新日期:1993-07-01 00:00:00
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journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-016-0274-7
更新日期:2017-02-01 00:00:00
abstract::Plasma chemistry, lipid metabolism and vitellogenin gene expression of captive Sterlet sturgeon Acipenser ruthenus were studied in different maturity stages. A total of 32 fish were sampled, and maturity stages were identified on the basis of histological criteria and direct observation. Females were classified to fou...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0189-8
更新日期:2016-06-01 00:00:00
abstract::Fish red blood cells (RBCs) exhibit an oxygen-dependent regulatory volume decrease (RVD) in hypoosmotic environment. In higher vertebrates, membrane-associated hemoglobin is involved in the regulation of osmotic ion movements across the cellular membrane. However, whether the hemoglobin conformational state plays a ro...
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更新日期:2019-12-01 00:00:00
abstract::To investigate the effect of water temperature on gamete maturation and gamete quality, European grayling (Thymalus thymallus) were held under different temperature regimes prior to spawning. Maturation of males and females and their gamete quality depended strongly on temperature regime. The highest percentages of ma...
journal_title:Fish physiology and biochemistry
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abstract::We have studied the location and the ontogeny of the digestive enzyme, phospholipase A2 (PLA2) immunohistochemically in the adult and larvae/juvenile of the red sea breamPagrus major by using an antiserum against theNaja naja venom PLA2. The antiserum reacts with at least one enzyme among the PLA2s purified from the f...
journal_title:Fish physiology and biochemistry
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更新日期:2010-12-01 00:00:00
abstract::In teleosts, cortisol is one of the key factors regulating the adaptation to environmental challenges, such as salinity changes. This paper compares interrenal function between fully adapted freshwater (FW) and sea water (SW) specimens of the euryhaline teleost Oreochromis mossambicus (tilapia), combining morphometric...
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abstract::The tissue distribution pattern of lipid is highly diverse among different fish species. Tiger puffer has a special lipid storage pattern, storing lipid predominantly in liver. In order to better understand the lipid physiology in fish storing lipid in liver, the present study preliminarily investigated the tissue dis...
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更新日期:2020-08-01 00:00:00
abstract::Obscure puffer (Takifugu obscurus) is an anadromous fish widely distributed around the coastal and inland rivers in East Asia. T. obscurus often encounters fluctuations in temperature and salinity. This study aimed to investigate the effect of the interactions of temperature and salinity on survival and oxidative stre...
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abstract::Three pepsinogens (PG1, PG2, and PG3) were highly purified from the stomach of freshwater fish rice field eel (Monopterus albus Zuiew) by ammonium sulfate fractionation and chromatographies on DEAE-Sephacel, Sephacryl S-200 HR. The molecular masses of the three purified PGs were all estimated as 36 kDa using SDS-PAGE....
journal_title:Fish physiology and biochemistry
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更新日期:2011-09-01 00:00:00
abstract::In this study we assessed the influence of three different environmental salinities (5, 15 and 31 psu during 90 days) on growth, osmoregulation, energy metabolism and digestive capacity in juveniles of the Notothenioid fish Eleginops maclovinus. At the end of experimental time samples of plasma, liver, gill, intestine...
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abstract::The growth of cyanobacteria (blue-green algae) is a typical phenomenon in water bodies worldwide. The use of silver carp (Hypophthalmichthys molitrix) to reduce excessive phytoplankton development is controversial. In the case of cyanobacteria, many of which are toxic, understanding their possible digestion mechanism ...
journal_title:Fish physiology and biochemistry
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abstract::The dependence of the effects of zinc and copper on the activities of proteinases of the stomach and intestinal mucosa on temperature and pH in four species of boreal piscivorous fish (pike Esox lucius, zander Zander lucioperca, perch Perca fluviatilis and burbot Lota lota) as well as in some of their potential preys ...
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