Abstract:
:Germinal vesicle migration (GVM) and/or dissolution (GVD) were measured in goldfish oocytes, treated with 17α, 20β dihydroxyprogesterone (DHP) and other compounds considered to effect the cytoskeleton and oxidative phosphorylation,in vitro. Administration of DHP reinitiated meiotic maturation, increasing GVM and GVD in goldfish oocytes. Addition of 2,4-dinitrophenol (DNP) to the incubation medium significantly inhibited DHP-induced GVM and GVD. The DNP effect was found to be partially reversible after 24 h and could be reversed fully after a further delay of approximately 24h. Treatment of goldfish oocytes with demecolcine (DE; a colchicine derivative also known as colcemid) induces GVM to the micropyle without effecting GVD; while Cytochalasin-B which inhibits microfilament polymerization impairs both GVM and GVD. Administration of DNP, significantly inhibited DE-induced GVM, suggesting that GVM as well as GVD are dependent upon the process of oxidative phosphorylation. Addition of adenosine-5' -triphosphate (ATP) at low concentrations (0.01-0.1 mM) did not effect DHP-induced or DNP-inhibited GVD in goldfish oocytes. The present results are consistent with the idea that migration of the oocyte nucleus during meiosis reinitiation has an energy requirement and involves participation by the cytoskeleton.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Habibi HR,Lessman CAdoi
10.1007/BF02311136subject
Has Abstractpub_date
1986-09-01 00:00:00pages
197-205issue
4eissn
0920-1742issn
1573-5168journal_volume
1pub_type
杂志文章abstract::Scrutiny of the zebrafish (Danio rerio) genomic database confirmed eight functional vitellogenin (vtg) genes, each with one or two transcript variants, and the encoded Vtg polypeptides were structurally and functionally characterized in detail by in silico and experimental analyses. There were five type I (vtgs1, 4, 5...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-018-0524-y
更新日期:2018-12-01 00:00:00
abstract::The incorporation and metabolism of (n-3) and (n-6) polyunsaturated fatty acids (PUFA) supplemented to growing cultures were studied in Atlantic salmon (AS) cells. A fatty acid concentration of 25 μM considerably altered the fatty acid composition of AS cells without increasing the neutral lipid content of the cells o...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00003426
更新日期:1990-07-01 00:00:00
abstract::Currently, the larviculture of many marine fish species with small-sized larvae depends for a short time after hatching, on the supply of high-quality live zooplankton to ensure high survival and growth rates. During the last few decades, the research community has made great efforts to develop artificial diets, which...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-018-0528-7
更新日期:2018-10-01 00:00:00
abstract::A study on the effect of three different anabolic-androgenic steroids on the growth, food conversion efficiency and nucleic acid contents of liver, kidney, brain and muscle of carp,Cyprinus carpio was undertaken. The three steroids, methyltestosterone (MT), ethylestrenol (EE) and oxandrolone (ON), were fed in differen...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01874771
更新日期:1989-05-01 00:00:00
abstract::For rainbow trout Oncorhynchus mykiss, high temperature is a major abiotic stress that limits its growth and productivity. In this study, spleen macrophage respiratory burst (RB), serum superoxide dismutase (SOD), serum malondialdehyde (MDA) and mRNA expression of the SERPINH1 (HSP47) gene in different tissues (liver,...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0170-6
更新日期:2016-04-01 00:00:00
abstract::Microcystin-LR (MCLR), one of the most popular microcystins (MCs) found in many field water bodies around the world, poses great health risks to animals and humans. In the present study, healthy common carp (initial weight 24.8 ± 2.3 g) were randomly assigned to five groups. Group I was fed on normal diet as control. ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章,随机对照试验
doi:10.1007/s10695-017-0354-3
更新日期:2017-08-01 00:00:00
abstract::The effects of chronic and periodic peaks of un-ionised ammonia (UIA-N) exposure on eye health and cataract formation in juvenile Atlantic halibut, Hippoglossus hippoglossus, were examined. Fish with mean initial weight 51.7 g (SD 13.2) were exposed to five treatments consisting of control group and three groups (Chro...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-011-9521-0
更新日期:2012-04-01 00:00:00
abstract::An 8-week growth trial was conducted to assess the effect of dietary protein on growth, feed utilization, protein retention efficiency, and body composition of young Heteropneustes fossilis (10.02 +/- 0.09 g; 9.93 +/- 0.07 cm). Isocaloric (4.15 kcal g(-1), GE) diets with varying levels of protein (25, 30, 35, 40, 45, ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-008-9273-7
更新日期:2009-08-01 00:00:00
abstract::Monitoring the growth of salmon during their early marine phase provides insights into prey availability, and growth rates may be linked to risks of size-dependent mortality. However, the measurement of growth rate is challenging for free-living salmon in the ocean. Insulin-like growth factor (IGF)-I is a growth-promo...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00681-y
更新日期:2019-12-01 00:00:00
abstract::Histo- and immunohistochemical techniques have recently been used to study the fibre type and myosin expression in fish muscle during development. In the present work, embryonic, larval and adult myosin isozymes (heavy and light chains) and parvalbumin isotypes were analyzed, from fertization to the adult stage, by po...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004524
更新日期:1992-08-01 00:00:00
abstract::This study was conducted to determine if diets supplemented with turmeric powder (Curcuma longa) affected the reversible sterilization of Pseudotropheus socolofi. Three experimental diets were formulated to contain 0%, 10%, and 14% turmeric powder. The fish (mean weight 13 g) were randomly divided into groups consisti...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-020-00781-0
更新日期:2020-08-01 00:00:00
abstract::The pattern of polyneuronal innervation in the sculpinMyoxocephalus scorpius was examined. The majority of fast fibres in sculpin are innervated by both adjacent nerves, and <15% receive input from sub-adjacent myotomes. Stimulation through both adjacent and sub-adjacent nerves results in muscle action potentials. Eac...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01881685
更新日期:1989-09-01 00:00:00
abstract::Plasma and liver composition, liver enzyme activities, and metabolite flux in isolated hepatocytes have been studied in immature American eels,Anguilla rostrata, injected daily IP with saline or cortisol (0.35 mg/kg eel). Plasma cortisol values were significantly increased above saline controls in those eels receiving...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF02290211
更新日期:1986-05-01 00:00:00
abstract::This study was conducted to identify embryonic stem cell (ESC) activities of a long-term cultured embryonic cell line previously derived from blastula-stage Oryzias dancena embryos. Five sub-cell lines were established from the embryonic cell line via clonal expansion of single cells. ESC activities, including clonoge...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0108-z
更新日期:2015-12-01 00:00:00
abstract::Blood being a vehicle for the transport of industrial pollutants in living system, fish hematology is considered as potent biomarker. In the present study, we investigated respective sublethal effects of pulp and paper mill effluents on hematology of two commonly cultured carps, Cyprinus carpio and Ctenopharyngodon id...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-016-0258-7
更新日期:2016-12-01 00:00:00
abstract::Obscure puffer (Takifugu obscurus) is an anadromous fish widely distributed around the coastal and inland rivers in East Asia. T. obscurus often encounters fluctuations in temperature and salinity. This study aimed to investigate the effect of the interactions of temperature and salinity on survival and oxidative stre...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-018-0537-6
更新日期:2019-02-01 00:00:00
abstract::The extensive use of pharmaceuticals in human and veterinary medicine may enter the aquatic environment and pose a serious threat to non-target aquatic organisms like fish. In this study, Indian major carp Cirrhinus mrigala was exposed to different concentrations (1, 10 and 100 μg L⁻¹) of most commonly used pharmaceut...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-013-9797-3
更新日期:2013-12-01 00:00:00
abstract::The aim of the present study was to explore the effect of waterborne zinc (control, 0.85, 2.20, 3.10 mg/l, respectively) exposure on lipid deposition and metabolism in the hepatopancreas and muscle of grass carp Ctenopharyngodon idella. The lipid content, Zn accumulation, and the activities and expression levels of se...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-016-0200-z
更新日期:2016-08-01 00:00:00
abstract::Virgin female rainbow trout, Oncorhynchus mykiss, were unilaterally ovariectomised at various stages of ovarian development to investigate the effect of the removal of one ovary on subsequent recruitment and growth of follicles in the remaining ovary. The right ovary was removed from groups of 12-15 fish, 12, 7 and 4 ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00003435
更新日期:1994-10-01 00:00:00
abstract::The effects of paraquat (PQ; 1,1'-dimethyl-4,4'-bipyridylium dichloride) treatment were investigated in carp, silver carp and wels. The serum glutamic-oxalacetic transaminase (GOT; L-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.1.1.) level was enhanced by 50% at 1 ppm exposure and by 100% at 10 ppm exposure in a...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004434
更新日期:1990-01-01 00:00:00
abstract::The aim of this study was to investigate the gene structure of two hormone-sensitive lipase b (HSLb) isoforms and their transcriptional regulation by insulin in grass carp (Ctenopharyngodon idella). HSL is an important lipolytic enzyme responsible for the hydrolysis of triacylglycerols (TAGs). Two isoforms (HSLa and H...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-016-0308-1
更新日期:2017-04-01 00:00:00
abstract::Ovarian cyp19a mRNA expression and P450 aromatase activity were measured in vivo in common carp Cyprinus carpio exposed to phenol for 96 h. Production of reactive oxygen species (ROS) and parameters of antioxidant defense system in serum ovary and liver of this fish after long-term phenol exposure were also studied. I...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0135-9
更新日期:2016-02-01 00:00:00
abstract::This study aimed to characterize the full-length cDNA of glucose-6-phosphate dehydrogenase (G6PD) from Megalobrama amblycephala with its responses to dietary carbohydrate levels characterized. The cDNA obtained covered 2768 bp with an open reading frame of 1572 bp. Sequence alignment and phylogenetic analysis revealed...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-018-0572-3
更新日期:2019-02-01 00:00:00
abstract::Calcium depletion/replacement studies were carried out to examine the role of calcium in contraction of trout intestinal smoot muscle in vitro. Three chemically distinct calcium channel blockers were used to determine whether voltage operated calcium channels (VOCs) were involved in calcium entry with either agonist o...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00003410
更新日期:1990-11-01 00:00:00
abstract::Variations in some enzyme activities during larval development of sea bass fed live prey were investigated from hatching to day 40. Fluctuations in the enzyme specific activities (except for trypsin) occurred in three phases: initially a sharp increase until day 12, followed by a plateau and subsequently a decrease ar...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004304
更新日期:1994-01-01 00:00:00
abstract::In this study, the mechanism that VC inhibits lipid deposition through GSK-3β/mTOR signaling was investigated in the liver of Danio rerio. The results indicated that 0.5- and 1.0-g/kg VC treatments activated mTOR signaling by inhibiting GSK-3β expression. The mRNA expression of FAS, ACC, and ACL, as well as the conten...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00727-1
更新日期:2020-02-01 00:00:00
abstract::Fish red blood cells (RBCs) exhibit an oxygen-dependent regulatory volume decrease (RVD) in hypoosmotic environment. In higher vertebrates, membrane-associated hemoglobin is involved in the regulation of osmotic ion movements across the cellular membrane. However, whether the hemoglobin conformational state plays a ro...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00689-4
更新日期:2019-12-01 00:00:00
abstract::The tilapia prolactin (PRL) cell responds rapidly (10-20 min) to small physiological changes in medium osmotic pressure (OP), releasing increasing quantities of hormone as medium OP is reduced. This release is rapidly (≤ 10 min) inhibited by somatostatin (SRIF). There is now extensive evidence that tilapia PRL cell fu...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004685
更新日期:1989-06-01 00:00:00
abstract::Teleosts have many spawning strategies and the hormonal control of gametogenesis is not well defined among the species or even, between sexes. To increase the knowledge of gonadotropin hormones, we studied the trend by gene expression of gonadotropin receptors in the follicles and testis at different maturity stages i...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-018-0479-z
更新日期:2018-06-01 00:00:00
abstract::In order to specify the timing of some changes in ovarian steroid production during the transition from vitellogenesis to ovulation, plasma hormones levels andin vivo andin vitro responses of the ovary to salmon gonadotropin (s-GtH) or dibutyryl-cyclic adenosine mono-phosphate (db-cAMP) were recorded in relationship w...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF02264076
更新日期:1986-10-01 00:00:00