Abstract：
:Fish red blood cells (RBCs) exhibit an oxygen-dependent regulatory volume decrease (RVD) in hypoosmotic environment. In higher vertebrates, membrane-associated hemoglobin is involved in the regulation of osmotic ion movements across the cellular membrane. However, whether the hemoglobin conformational state plays a role in the regulation of osmotic responses in fish red blood cells is still not fully understood. We found that changes in hemoglobin conformation influence the pattern of the regulatory volume decrease in Carassius carassius red blood cells. In oxygenated cells (96.4 ± 3.7% oxygenated hemoglobin), the volume recovery was completed within 125 min. Deoxygenation of hemoglobin (96.5 ± 2.7% of deoxygenated hemoglobin) inhibited the volume decrease in hyposmotically swollen red blood cells. Reoxygenation restored regulatory volume decrease in cells within 5 min. Induced methemoglobinemia (48.4 ± 1.8% of methemoglobin and 41.3 ± 2.3% of deoxygenated hemoglobin) blocked the process of volume recovery and significantly decreased osmotic stability of red blood cells.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Andreyeva AY,Soldatov AA,Krivchenko AI,Mindukshev IV,Gambaryan Sdoi
10.1007/s10695-019-00689-4subject
Has Abstractpub_date
2019-12-01 00:00:00pages
1933-1940issue
6eissn
0920-1742issn
1573-5168pii
10.1007/s10695-019-00689-4journal_volume
45pub_type
杂志文章abstract：:The objective of the present study was to confirm previous results on the mediation of GnRH signal in tilapia by providing evidence from experiments in cultured pituitary cells and from perifusion experiments using a GnRH-antagonist. After 4 days in culture under identical conditions, cells taken from pituitaries of f...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004550
更新日期：1993-07-01 00:00:00
abstract：:This study was conducted to determine if diets supplemented with turmeric powder (Curcuma longa) affected the reversible sterilization of Pseudotropheus socolofi. Three experimental diets were formulated to contain 0%, 10%, and 14% turmeric powder. The fish (mean weight 13 g) were randomly divided into groups consisti...
journal_title：Fish physiology and biochemistry
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abstract：:Individual differences in behaviour and physiological responses to stress are associated with evolutionary adaptive variation and thus raw material for evolution. In farmed animals, the interest in consistent trait associations, i.e. coping styles, has increased dramatically over the last years. However, one of limita...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-016-0231-5
更新日期：2016-10-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-013-9822-6
更新日期：2014-02-01 00:00:00
abstract：:This study aimed to investigate the stress response of Sparus aurata specimens fed with nutraceutical aquafeed brewer's yeast (Saccharomyces cerevisiae) and spirulina (Arthrospira platensis). For that purpose, 96 (169.0 ± 2.8 g) animals were distributed randomly in 12 tanks (8 fish per tank, 4 replicates) and divided ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00677-8
更新日期：2019-12-01 00:00:00
abstract：:The present study explored the protective role of dietary the extract of Angelica sinensis (EAs) on high density, CuSO4, or trichlorfon-treated Crucian carp (Carassius auratus auratus). Firstly, the study showed that the optimum density for growth and growth inhibition was 0.49 and 0.98 fish L-1 water, respectively. D...
journal_title：Fish physiology and biochemistry
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更新日期：2020-10-01 00:00:00
abstract：:In vertebrate species, the biopotential embryonic gonad differentiation is affected by many key genes and key steroidogenic enzymes. Insulin-like growth factor-1 receptor (Igf1r) has been considered as an important sex-differentiation gene in mammals and could mediate the biological action of Igf1, an important regula...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-014-9914-y
更新日期：2014-08-01 00:00:00
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journal_title：Fish physiology and biochemistry
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doi：10.1007/s10695-015-0135-9
更新日期：2016-02-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章,随机对照试验
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更新日期：2018-02-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-009-9355-1
更新日期：2010-06-01 00:00:00
abstract：:Trypsin from the viscera of Bogue (Boops boops) was purified to homogeneity by precipitation with ammonium sulphate, Sephadex G-100 gel filtration and Mono Q-Sepharose anion exchange chromatography, with an 8.5-fold increase in specific activity and 36% recovery. The molecular weight of the purified enzyme was estimat...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-009-9365-z
更新日期：2010-12-01 00:00:00
abstract：:Animal and/or plant protein sources substituting fishmeal in the diets keep being developed due to its high price. The purpose of this study is to determine response of dietary substitution of fishmeal with silkworm pupae meal, promate meal®, meat and bone meal and/or their combination on the performance of juvenile o...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9555-3
更新日期：2012-06-01 00:00:00
abstract：:In this study, for the first time, both neuropeptides isotocin (IT) and arginine vasotocin (AVT) have been identified and measured in urophysis, the neurohaemal organ of the caudal neurosecretory system of teleost fish. So far, AVT, but not IT, was quantified by radioimmunoassay (RIA) in urophysis of several fish spec...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-012-9746-6
更新日期：2013-08-01 00:00:00
abstract：:Rainbow trout were infused continuously for 12h with epinephrine in the presence or absence of alpha-and/or beta-adrenergic blockade to characterize the specific adrenergic mechanisms involved in the control of blood acid-base status and oxygen transport capacity. Infusion of epinephrine, alone, produced a transient r...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01871745
更新日期：1988-01-01 00:00:00
abstract：:Plasma chemistry, lipid metabolism and vitellogenin gene expression of captive Sterlet sturgeon Acipenser ruthenus were studied in different maturity stages. A total of 32 fish were sampled, and maturity stages were identified on the basis of histological criteria and direct observation. Females were classified to fou...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0189-8
更新日期：2016-06-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01874771
更新日期：1989-05-01 00:00:00
abstract：:A 90-day randomized feeding experiment was performed to assess the effects of dietary cobalt (Co) supplementation on the growth performance, muscle composition, status of iron and manganese in the muscle as well as the expression of growth-related genes in the muscle (myoblast determination protein 1 homolog (MyoD) an...
journal_title：Fish physiology and biochemistry
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doi：10.1007/s10695-019-00723-5
更新日期：2020-02-01 00:00:00
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journal_title：Fish physiology and biochemistry
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更新日期：2014-12-01 00:00:00
abstract：:Protein digestibility by gastrointestinal homogenates of Dicentrarchus labrax, was investigated in 5 feeds in relation to their amino acids composition and their ability to sustain growth of D. labrax post larval juveniles. Gastric proteolysis was found to contribute <25% of the total proteolytic capacity. Correlation...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
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更新日期：1995-10-01 00:00:00
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更新日期：2009-11-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
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更新日期：1994-06-01 00:00:00
abstract：:This study was performed to unravel the mechanism of the beneficial action of taurine on marine teleost fish, red sea bream (Pagrus major), by analyzing the hepatic metabolism. Moreover, the ameliorative effects of the nutrient against cadmium toxicity and bioaccumulation were further evaluated. The fish were fed a di...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
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更新日期：2017-02-01 00:00:00
abstract：:The purpose of the current study was to examine seasonal changes in seawater tolerance and growth performance of anadromous Arctic charr (Salvelinus alpinus L.) held at the same temperature (8°C) during winter and summer. Charr (20-27 cm), previously reared in freshwater under natural photoperiod, were transferred eit...
journal_title：Fish physiology and biochemistry
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更新日期：1993-12-01 00:00:00
abstract：:Lectins are sugar-binding proteins that mediate pathogen recognition and cell-cell interactions. A rhamnose-binding lectin (RBL) gene and its promoter region have been cloned and characterized from snakehead Channa argus. From the transcription initiation site, snakehead rhamnose-binding lectin (SHL) gene extends 2,38...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
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更新日期：2010-09-01 00:00:00
abstract：:In vitro studies have suggested that connexin43 (cx43) expression is of particular importance during establishment and regeneration of the mammalian hematopoietic system. However, little is known about its in vivo functions during hematopoiesis due to the embryonic lethality of mammalian knockout models. In this study...
journal_title：Fish physiology and biochemistry
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更新日期：2010-12-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
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更新日期：1993-07-01 00:00:00
abstract：:In the present study, heat shock cognate 70 (HSC70) and inducible heat shock protein 70 (HSP70) gene of humphead snapper, Lutjanus sanguineus, were cloned by rapid amplification of cDNA ends (RACE) technique with the primers designed from the known expressed sequence tags (ESTs) identified from the subtracted cDNA lib...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9487-y
更新日期：2011-12-01 00:00:00
abstract：:We have studied the location and the ontogeny of the digestive enzyme, phospholipase A2 (PLA2) immunohistochemically in the adult and larvae/juvenile of the red sea breamPagrus major by using an antiserum against theNaja naja venom PLA2. The antiserum reacts with at least one enzyme among the PLA2s purified from the f...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF02274224
更新日期：1992-02-01 00:00:00
abstract：:The main indication of cimetidine is being H₂-receptor antagonist, but studies suggest that cimetidine may also act as a non-specific stimulant of cell-mediated immunity and immunomodulator. In order to determine the immunomodulatory effect of dietary intake of cimetidine in the common carp (100 ± 10 g), subjects were...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-013-9804-8
更新日期：2013-12-01 00:00:00
