Abstract：
:Trypsin from the viscera of Bogue (Boops boops) was purified to homogeneity by precipitation with ammonium sulphate, Sephadex G-100 gel filtration and Mono Q-Sepharose anion exchange chromatography, with an 8.5-fold increase in specific activity and 36% recovery. The molecular weight of the purified enzyme was estimated to be 23 kDa by SDS-PAGE and size exclusion chromatography. The purified trypsin appeared as a single band on native-PAGE and zymography staining. The purified enzyme showed esterase-specific activity on N-α-benzoyl-L-arginine ethyl ester (BAEE) and amidase activity on N-α-benzoyl-DL-arginine-p-nitroanilide (BAPNA). The optimum pH and temperature for the enzyme activity, after 10 min incubation, were pH 9.0 and 55°C, respectively, using BAPNA as a substrate. The trypsin kinetic constants Km and kcat on BAPNA were 0.13 mM and 1.56 s(-1), respectively, while the catalytic efficiency kcat/Km was 12 s(-1) mM(-1). Biochemical characterisation of B. boops trypsin showed that this enzyme can be used as a possible biotechnological tool in the fish processing and food industries.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Barkia A,Bougatef A,Nasri R,Fetoui E,Balti R,Nasri Mdoi
10.1007/s10695-009-9365-zsubject
Has Abstractpub_date
2010-12-01 00:00:00pages
893-902issue
4eissn
0920-1742issn
1573-5168journal_volume
36pub_type
杂志文章abstract：:Evidence has recently been presented for variation in the inducibility of various 7-alkoxycoumarin-O-dealkylase activities in liver microsomes from a number of mammalian species by β-naphthoflavone (βNF). In the present study we have investigated the inducibility of hepatic microsomal 7-methoxycoumarin-O-demethylase, ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01875022
更新日期：1989-07-01 00:00:00
abstract：:In this study, for the first time, both neuropeptides isotocin (IT) and arginine vasotocin (AVT) have been identified and measured in urophysis, the neurohaemal organ of the caudal neurosecretory system of teleost fish. So far, AVT, but not IT, was quantified by radioimmunoassay (RIA) in urophysis of several fish spec...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-012-9746-6
更新日期：2013-08-01 00:00:00
abstract：:Plasma chemistry, lipid metabolism and vitellogenin gene expression of captive Sterlet sturgeon Acipenser ruthenus were studied in different maturity stages. A total of 32 fish were sampled, and maturity stages were identified on the basis of histological criteria and direct observation. Females were classified to fou...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0189-8
更新日期：2016-06-01 00:00:00
abstract：:Variations in some enzyme activities during larval development of sea bass fed live prey were investigated from hatching to day 40. Fluctuations in the enzyme specific activities (except for trypsin) occurred in three phases: initially a sharp increase until day 12, followed by a plateau and subsequently a decrease ar...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004304
更新日期：1994-01-01 00:00:00
abstract：:The tilapia prolactin (PRL) cell responds rapidly (10-20 min) to small physiological changes in medium osmotic pressure (OP), releasing increasing quantities of hormone as medium OP is reduced. This release is rapidly (≤ 10 min) inhibited by somatostatin (SRIF). There is now extensive evidence that tilapia PRL cell fu...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004685
更新日期：1989-06-01 00:00:00
abstract：:The present study aimed to assess the sewage effects of the Brazilian Antarctic Station Comandante Ferraz, Admiralty Bay, King George Island, on the hepatic metabolism (energetic, antioxidant, and arginase levels) and levels of plasma constituents of two Antarctic fish species Notothenia rossii and N. coriiceps. The b...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0080-7
更新日期：2015-10-01 00:00:00
abstract：:Histo- and immunohistochemical techniques have recently been used to study the fibre type and myosin expression in fish muscle during development. In the present work, embryonic, larval and adult myosin isozymes (heavy and light chains) and parvalbumin isotypes were analyzed, from fertization to the adult stage, by po...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004524
更新日期：1992-08-01 00:00:00
abstract：:Spotted wolffish Anarhichas minor reproduction in captivity is dependent on in vitro fertilization. However, it is often challenging to acquire sufficient fresh sperm to fertilize the eggs that are obtained. In this study, we evaluate the possibility to store spotted wolffish sperm by refrigeration. Spotted wolffish s...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-020-00820-w
更新日期：2020-06-02 00:00:00
abstract：:In the present study, three cDNAs of AdipoRs (MpAdipoR1a, MpAdipoR1b, and MpAdipoR2) were identified from juvenile black carp Mylopharyngodon piceus. There were 375, 378, and 356 amino acids in the MpAdipoR1a, MpAdipoR1b, and MpAdipoR2, respectively. BLAST analysis reveals that MpAdipoRs share high identities with oth...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-018-0501-5
更新日期：2018-08-01 00:00:00
abstract：:In this study, a freshwater fish Channa punctatus was exposed to subacute concentrations of synthetic pyrethroid insecticides (cypermethrin and lambda-cyhalothrin) for 96 h to evaluate their impact on the levels of nucleic acids and protein in its different organs. Significant enhancement in the level of DNA was recor...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-007-9192-z
更新日期：2008-12-01 00:00:00
abstract：:Enzymatic changes that occur in the white somatic muscle of rainbow trout (Oncorhynchus mykiss) in response to spawning were investigated, and the evenness of their distribution across the ventral-dorsal plane of this muscle was assessed. Four enzymes that are involved in energy metabolism were measured (phosphofructo...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004344
更新日期：1995-12-01 00:00:00
abstract：:The plasma levels of estradiol-17β (E2), 17α, 20β-dihydroxy-4-pregnen-3-one (17,20-P) and gonadotropin (GTH) were measured in brook trout (Salvelinus fontinalis) during the period from the end of vitellogenesis to postovulation. Blood samples were taken according to specific stages of maturation, including germinal ve...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF02180281
更新日期：1987-06-01 00:00:00
abstract：:The activities of trypsin (EC 3.4.21.4), chymotrypsin (EC 3.4.21.1), lipase (EC 3.1.1.3) and amylase (EC 3.2.1.1) were measured in different regions of the alimentary tract of ammocoetes from each of the three extant lamprey families. In the southern hemisphere speciesGeotria australis (Geotriidae), and even more part...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004649
更新日期：1992-05-01 00:00:00
abstract：:An immunohistochemical study of the sturgeon (Acipenser baeri) pituitary was undertaken using antisera directed against hormones from various classes of vertebrates, including the only pituitary hormone available from sturgeon, gonadotrophin. A positive reaction was obtained after application of antisera towards the f...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01875699
更新日期：1988-07-01 00:00:00
abstract：:Microcystin-LR (MCLR), one of the most popular microcystins (MCs) found in many field water bodies around the world, poses great health risks to animals and humans. In the present study, healthy common carp (initial weight 24.8 ± 2.3 g) were randomly assigned to five groups. Group I was fed on normal diet as control. ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10695-017-0354-3
更新日期：2017-08-01 00:00:00
abstract：:The cardioventilatory performance of the Japanese eel,Anguilla japonica, was evaluated during acute exposure to hypoxia. The eel became an oxygen conformer as ambient PO2 fell below the critical value of 110 mmHg. Although arterial and venous PO2 also fell progressively, the arterial O2 content remained constant down ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF02264086
更新日期：1986-10-01 00:00:00
abstract：:We have studied the seasonal relationship between growth and circulating growth hormone (GH), hepatic GH-binding and plasma insulin-like growth factor-I immunoreactivity in gilthead sea bream,Sparus aurata. The seasonal increase in plasma GH levels preceded by several weeks the summer increase in growth rates. In cont...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004358
更新日期：1994-07-01 00:00:00
abstract：:In mammals, androgens appear to enhance the development of primary ovarian follicles, but PI3K (phosphoinositide 3-kinases) pathway is well recognized as one of the critical pathways in early follicular development. Roles of the PI3K were revealed by deletion of PTEN (phosphatase and tensin homolog on chromosome 10). ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9560-6
更新日期：2012-06-01 00:00:00
abstract：:The present study investigates the nephrotoxic effects of two acute doses of silver nanoparticles (AgNPs) and silver nitrate (AgNO3) on the African catfish, Clarias gariepinus, using biochemical, histochemical, and histopathological changes as biomarkers. AgNP-induced impacts were recorded in some of these characteris...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00686-7
更新日期：2019-12-01 00:00:00
abstract：:Changes in serum thyroxine (T4), estradiol-17β (E2) and testosterone (T) levels during the parr-smolt transformation (smoltification) were investigated in the masu salmon (Oncorhynchus masou) in 1985 and 1987. T4 showed a peak in levels at the early stage of smoltification and E2 and T levels peaked almost at the same...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004317
更新日期：1993-07-01 00:00:00
abstract：:Measurement of serum biochemical parameters in response to metal exposures can be especially useful to help identify target organs of toxicity as well as the general health status of animals. Oreochromis niloticus were exposed to 5.0 mg/L Zn, 1.0 mg/L Cd, and 5.0 mg/L Zn + 1.0 mg/L Cd mixture for 7 and 28 days, and al...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-009-9337-3
更新日期：2010-09-01 00:00:00
abstract：:The proline-rich protein 13 (PRR13) is reported to be a key regulator of the resistance to cytostatica by decreasing the copy number of the proapoptotic gene thrombospondin-1. We isolated and characterized the complete PRR13 gene sequence of rainbow trout (Oncorhynchus mykiss). The gene comprises four exons and three ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-010-9407-6
更新日期：2010-12-01 00:00:00
abstract：:Connective tissue growth factor (ctgf) is involved in the proliferation, migration, adhesion of cell, and the constituent of extracellular matrix, which plays an important role in embryogenesis, angiogenesis, wound repair, and fibrosis diseases. In this study, the cDNA sequence of grass carp ctgf gene was cloned by ra...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00653-2
更新日期：2019-08-01 00:00:00
abstract：:The purpose of the current study was to examine seasonal changes in seawater tolerance and growth performance of anadromous Arctic charr (Salvelinus alpinus L.) held at the same temperature (8°C) during winter and summer. Charr (20-27 cm), previously reared in freshwater under natural photoperiod, were transferred eit...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004413
更新日期：1993-12-01 00:00:00
abstract：:Fibroblast growth factors (FGFs) have been proved to participate in a wide variety of processes, including growth, differentiation, cell proliferation, migration, sex determination and sex differentiation. The roles of FGF9/16/20 subfamily members in the gonadal development of teleost fish have not yet been reported. ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-012-9630-4
更新日期：2012-10-01 00:00:00
abstract：:We have developed a novel single cell real-time quantitative PCR technique, which incorporates harvesting marker-identified single cells using laser-capture. Here, for the first time in a vertebrate species, using this innovative single cell gene profiling technique, we report the presence of G-protein coupled recepto...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-006-0018-1
更新日期：2005-04-01 00:00:00
abstract：:Levels of catecholamines and tyrosine hydroxylase (TH) activity were measured in brain homogenates from female rainbow trout. In triploid fish or in diploid fish in ovarian recrudescence, the patterns of catecholamine content expressed as a function of in vitro TH activity vary in different areas of the brain. Km for ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004560
更新日期：1993-07-01 00:00:00
abstract：:Sperm quality parameters in rainbow trout (Oncorhynchus mykiss) were investigated during normal season spawning (November-January) and out-season spawning (July-August) treated with artificial photoperiod manipulation. Normal spawning males (n = 15) were kept in an open concrete pond under natural condition. Out-seaso...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-018-0564-3
更新日期：2018-12-01 00:00:00
abstract：:The analysis of the tench growth hormone gene structure revealed a comparable organization of coding and non-coding regions than other from cyprinid species. Based on the performed mRNA and amino acid sequence alignments, gh tench is related to Asian than to European representatives of Cyprinidae family. Second aim of...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-012-9661-x
更新日期：2012-12-01 00:00:00
abstract：:A series of biochemical determination and electrophoretic observations have been conducted to analyze the activities and characteristics of protease, α-amylase, and lipase of paddlefish Polyodon spathula. The results obtained have been compared with those of bighead carp (Aristichthys nobilis) and hybrid sturgeon (Hus...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9541-9
更新日期：2012-06-01 00:00:00
