Abstract：
:We have developed a novel single cell real-time quantitative PCR technique, which incorporates harvesting marker-identified single cells using laser-capture. Here, for the first time in a vertebrate species, using this innovative single cell gene profiling technique, we report the presence of G-protein coupled receptors in individual gonadotropin-releasing hormone (GnRH) neurons and endocrine cells of the pituitary of the tilapia Oreochromis niloticus. The differential expression of multiple combinations of three GnRH receptor types (R1, R2 and R3) in individual gonadotropic and nongonadotropic cells demonstrates cellular and functional heterogeneity. The differential use of GnRH receptors in corticotropes, melanotropes and thyrotropes during gonadal maturation and reproductive behaviors suggests new roles for these hormones. Further, we provide evidence of the structure of a novel nonmammalian G-protein coupled receptor (GPR54) for kisspeptins, encoded by Kiss-1 gene, which is highly conserved during evolution and expressed in GnRH1, GnRH2 and GnRH3 neurons. We hypothesize GPR54 stimulates GnRH secretion and is crucial for pubertal maturation. We speculate, the use of this method will allow the identification and quantification of known and unknown genes in single cells, which would greatly facilitate our understanding of the complex interactions that govern the physiology of individual cells in vertebrates species.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Parhar ISdoi
10.1007/s10695-006-0018-1subject
Has Abstractpub_date
2005-04-01 00:00:00pages
153-6issue
2-3eissn
0920-1742issn
1573-5168journal_volume
31pub_type
杂志文章abstract：:An 8-week feeding trial was performed to test the effects of glycyrrhetinic acid (GA) on growth and some gene expression of hepatic lipid metabolism in channel catfish (initial body weight, 3.5 ± 0.02 g) fed high-fat diets. Fish were fed the control diet, high-fat diet (HFD), and HFD supplemented with 0.4, 0.8, and 1....
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00740-4
更新日期：2020-04-01 00:00:00
abstract：:In the present study, heat shock cognate 70 (HSC70) and inducible heat shock protein 70 (HSP70) gene of humphead snapper, Lutjanus sanguineus, were cloned by rapid amplification of cDNA ends (RACE) technique with the primers designed from the known expressed sequence tags (ESTs) identified from the subtracted cDNA lib...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9487-y
更新日期：2011-12-01 00:00:00
abstract：:Cytochrome P450 aromatase, which is encoded by the CYP19a gene, converts androgens to estradiol. Considerable evidence suggests that estrogens play an important role in fish reproductive process. Therefore CYP19a is an excellent candidate gene for reproductive traits. Variants in the promoter of the CYP19a gene might ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-008-9237-y
更新日期：2009-08-01 00:00:00
abstract：:Due to rapid depletion of wild stocks, the necessity to cultivate fish is eminent. Current fish farming practices seek to improve flesh quality. The notion that white muscles are the main target of the fishing industry is emphasized. A novel approach is suggested based on the development of white muscles in wild fish ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9501-4
更新日期：2011-06-01 00:00:00
abstract：:A 90-day randomized feeding experiment was performed to assess the effects of dietary cobalt (Co) supplementation on the growth performance, muscle composition, status of iron and manganese in the muscle as well as the expression of growth-related genes in the muscle (myoblast determination protein 1 homolog (MyoD) an...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00723-5
更新日期：2020-02-01 00:00:00
abstract：:Red crucian carp (Carassius auratus red var.) is an ornamental fish with vivid red/orange color. It has been found that the adult body color of this strain forms a gray-to-red change. In this study, skin transcriptomes of red crucian carp are first obtained for three different stages of body color development, named b...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-017-0379-7
更新日期：2017-10-01 00:00:00
abstract：:The present study was conducted to investigate the effects of high dietary lipid levels on growth, metabolism, antioxidant capacity, and immune responses of largemouth bass. Fish (initial body weight 13.38 ± 0.11 g) were fed three isonitrogenous semi-purified diets containing 5%, 10%, and 20% lipid, respectively. The ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00705-7
更新日期：2020-02-01 00:00:00
abstract：:This study was conducted to determine if diets supplemented with turmeric powder (Curcuma longa) affected the reversible sterilization of Pseudotropheus socolofi. Three experimental diets were formulated to contain 0%, 10%, and 14% turmeric powder. The fish (mean weight 13 g) were randomly divided into groups consisti...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-020-00781-0
更新日期：2020-08-01 00:00:00
abstract：:It is shown that amylolytic and proteolytic activity of the intestinal mucosa, the chyme and the intestinal flora in the fishes, zander Zander lucioperca (L.), perch Perca fluviatilis L., bream Abramis brama (L.) and roach Rutilus rutilus (L.), belonging according to their feeding habits to different ecological groups...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-010-9426-3
更新日期：2011-09-01 00:00:00
abstract：:Plasma levels of L-thyroxine (T4) and 3,5,3'-triiodo-L-thyronine (T3) and the percentage of plasma T4 and T3 present in the free (dialyzable) form (%FT4 and %FT3) were measured in 16 species (11 families) of tropical marine teleosts from an inshore Barbados reef. Mean plasma T4 varied from 0.2 ng/ml to 42 ng/ml; mean ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF02180414
更新日期：1987-05-01 00:00:00
abstract：:Plasma and liver composition, liver enzyme activities, and metabolite flux in isolated hepatocytes have been studied in immature American eels,Anguilla rostrata, injected daily IP with saline or cortisol (0.35 mg/kg eel). Plasma cortisol values were significantly increased above saline controls in those eels receiving...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF02290211
更新日期：1986-05-01 00:00:00
abstract：:To investigate the effect of water temperature on gamete maturation and gamete quality, European grayling (Thymalus thymallus) were held under different temperature regimes prior to spawning. Maturation of males and females and their gamete quality depended strongly on temperature regime. The highest percentages of ma...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9526-8
更新日期：2012-04-01 00:00:00
abstract：:Histological development of the digestive tract and specific activities of three digestive enzymes (trypsin, alkaline phosphatase, and pepsin) were studied in rock bream Oplegnathus fasciatus from hatching to 50 days after hatching (DAH). At hatching, the digestive tract appeared as an undifferentiated straight tube a...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9507-y
更新日期：2012-04-01 00:00:00
abstract：:An immunohistochemical study of the sturgeon (Acipenser baeri) pituitary was undertaken using antisera directed against hormones from various classes of vertebrates, including the only pituitary hormone available from sturgeon, gonadotrophin. A positive reaction was obtained after application of antisera towards the f...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01875699
更新日期：1988-07-01 00:00:00
abstract：:High-mobility group box 2 (HMGB2) is a nonhistone architectural protein that plays important roles in many biological processes. In this study, we cloned a homologue of the HMGB2 from the lymphocyte-like cells of Lampetra japonica (L. japonica). Sequence analysis reveals that L. japonica HMGB2 contains two highly cons...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-013-9871-x
更新日期：2014-04-01 00:00:00
abstract：:The development of digestive enzymes during the early ontogeny of the Mayan cichlid (Cichlasoma urophthalmus) was studied using biochemical and electrophoretic techniques. From yolk absorption (6 days after hatching: dah), larvae were fed Artemia nauplii until 15 dah, afterward they were fed with commercial microparti...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-010-9431-6
更新日期：2011-03-01 00:00:00
abstract：:In order to specify the timing of some changes in ovarian steroid production during the transition from vitellogenesis to ovulation, plasma hormones levels andin vivo andin vitro responses of the ovary to salmon gonadotropin (s-GtH) or dibutyryl-cyclic adenosine mono-phosphate (db-cAMP) were recorded in relationship w...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF02264076
更新日期：1986-10-01 00:00:00
abstract：:The retinoid X receptors (RXRs) are involved in the skeletal development and other biological process such as blood vessel formation and metabolism. Partial sequences of RXRα and β genes were obtained, and their expressions were quantified on golden pompano Trachinotus ovatus at 28 days post hatching (DPH) to explore ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0098-x
更新日期：2015-12-01 00:00:00
abstract：:Prolactin (PRL) is one of the most versatile hormones found in the pituitary of vertebrates and exerts its actions through binding to a specific PRL receptor (PRLR). Here we describe the cloning and characterization of a second prolactin receptor (ntPRLR2), isolated from the ovary of Nile tilapia (Oreochromis niloticu...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-009-9355-1
更新日期：2010-06-01 00:00:00
abstract：:Fibroblast growth factors (FGFs) have been proved to participate in a wide variety of processes, including growth, differentiation, cell proliferation, migration, sex determination and sex differentiation. The roles of FGF9/16/20 subfamily members in the gonadal development of teleost fish have not yet been reported. ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-012-9630-4
更新日期：2012-10-01 00:00:00
abstract：:The tilapia prolactin (PRL) cell responds rapidly (10-20 min) to small physiological changes in medium osmotic pressure (OP), releasing increasing quantities of hormone as medium OP is reduced. This release is rapidly (≤ 10 min) inhibited by somatostatin (SRIF). There is now extensive evidence that tilapia PRL cell fu...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004685
更新日期：1989-06-01 00:00:00
abstract：:This study was conducted to determine the effects of dietary supplementation of lemon balm (Melissa officinalis) aqueous methanolic extract on growth performance, blood parameters, digestive and antioxidant enzyme activities, and non-specific immune responses in rainbow trout (Oncorhynchus mykiss). Fish with an averag...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00737-z
更新日期：2020-02-01 00:00:00
abstract：:Development of the digestive tract and accessory glands of larvae of the fat snook Centropomus parallelus was examined under light microscopy, from hatching to 60 day post-hatching (dph). At hatching, the digestive tract is straight and composed by a cubic cell layer. The exogenous feeding starts at 3 dph, concomitant...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0028-y
更新日期：2015-04-01 00:00:00
abstract：:This study aimed to investigate the stress response of Sparus aurata specimens fed with nutraceutical aquafeed brewer's yeast (Saccharomyces cerevisiae) and spirulina (Arthrospira platensis). For that purpose, 96 (169.0 ± 2.8 g) animals were distributed randomly in 12 tanks (8 fish per tank, 4 replicates) and divided ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00677-8
更新日期：2019-12-01 00:00:00
abstract：:We have studied the location and the ontogeny of the digestive enzyme, phospholipase A2 (PLA2) immunohistochemically in the adult and larvae/juvenile of the red sea breamPagrus major by using an antiserum against theNaja naja venom PLA2. The antiserum reacts with at least one enzyme among the PLA2s purified from the f...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF02274224
更新日期：1992-02-01 00:00:00
abstract：:Trypsin from the viscera of Bogue (Boops boops) was purified to homogeneity by precipitation with ammonium sulphate, Sephadex G-100 gel filtration and Mono Q-Sepharose anion exchange chromatography, with an 8.5-fold increase in specific activity and 36% recovery. The molecular weight of the purified enzyme was estimat...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-009-9365-z
更新日期：2010-12-01 00:00:00
abstract：:The pineal organ of fish, through its 24h rhythmic release of melatonin, acts as a transducer of the photoperiod, influencing different physiological functions (e.g., reproduction, growth). The target sites for melatonin are poorly known in fish, especially marine species. A radioligand study was undertaken using the ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01875583
更新日期：1996-11-01 00:00:00
abstract：:An 8-week growth trial was conducted to assess the effect of dietary protein on growth, feed utilization, protein retention efficiency, and body composition of young Heteropneustes fossilis (10.02 +/- 0.09 g; 9.93 +/- 0.07 cm). Isocaloric (4.15 kcal g(-1), GE) diets with varying levels of protein (25, 30, 35, 40, 45, ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-008-9273-7
更新日期：2009-08-01 00:00:00
abstract：:The proline-rich protein 13 (PRR13) is reported to be a key regulator of the resistance to cytostatica by decreasing the copy number of the proapoptotic gene thrombospondin-1. We isolated and characterized the complete PRR13 gene sequence of rainbow trout (Oncorhynchus mykiss). The gene comprises four exons and three ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-010-9407-6
更新日期：2010-12-01 00:00:00
abstract：:The dependence of the effects of zinc and copper on the activities of proteinases of the stomach and intestinal mucosa on temperature and pH in four species of boreal piscivorous fish (pike Esox lucius, zander Zander lucioperca, perch Perca fluviatilis and burbot Lota lota) as well as in some of their potential preys ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-009-9353-3
更新日期：2010-09-01 00:00:00
