Abstract:
:This study was conducted to identify embryonic stem cell (ESC) activities of a long-term cultured embryonic cell line previously derived from blastula-stage Oryzias dancena embryos. Five sub-cell lines were established from the embryonic cell line via clonal expansion of single cells. ESC activities, including clonogenicity, alkaline phosphatase (AP) activity, and differentiation capacity, were examined in the five sub-cell lines. We observed both clonogenicity and AP activity in all five sub-cell lines, but the proportion of cells that exhibited both properties was significantly different among them. Even though we detected different formation rates and sizes of embryoid body (EB) among these cells, all lines were stably able to form EBs and further induction for differentiation showed their capability to differentiate into other cell types in a spontaneous manner. From this study, we determined that the embryonic cell lines examined possessed heterogeneous ESC activities and can be utilized as a marine model system for fish ESC-based research.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Lee D,Ryu JH,Lee ST,Nam YK,Kim DS,Gong SPdoi
10.1007/s10695-015-0108-zsubject
Has Abstractpub_date
2015-12-01 00:00:00pages
1569-76issue
6eissn
0920-1742issn
1573-5168pii
10.1007/s10695-015-0108-zjournal_volume
41pub_type
杂志文章abstract::Plasma amino acid profiles in the systemic circulation of rainbow trout acclimated to diets containing different protein sources, with and without supplementation with free amino acids, were monitored for up to 120 hours after consumption of the diets. Plasma concentrations of lysine, methionine, and isoleucine increa...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004309
更新日期:1995-06-01 00:00:00
abstract::The pseudobranch in rainbow trout was investigated in order to clarify its metabolic and physiological function. Contents of lactate, glycogen, and glycerides and activities of carbonic anhydrase (CA), lactate dehydrogenase (LDH), and 3-hydroxy-acyl CoA dehydrogenase (HAD) were measured and compared with those in the ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004070
更新日期:1995-08-01 00:00:00
abstract::In the present study, different reference genes were isolated, and their stability in the skeletal muscle of fine flounder subjected to different nutritional states was assessed using geNorm and NormFinder. The combinations between 18S and ActB; Fau and 18S; and Fau and Tubb were chosen as the most stable gene combina...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-012-9739-5
更新日期:2013-08-01 00:00:00
abstract::The composition of seminal plasma and metabolism of sperm of the cyprinid fishAlburnus alburnus were investigated. Statistically significant correlations were found between motility parameters and seminal fluid osmolality, pH, Na(+), K(+) and protein levels (negative correlations: % immotile spermatozoa-Na(+), K(+); p...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01875596
更新日期:1996-04-01 00:00:00
abstract::Protein digestibility by gastrointestinal homogenates of Dicentrarchus labrax, was investigated in 5 feeds in relation to their amino acids composition and their ability to sustain growth of D. labrax post larval juveniles. Gastric proteolysis was found to contribute <25% of the total proteolytic capacity. Correlation...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00003377
更新日期:1995-10-01 00:00:00
abstract::This study was performed to unravel the mechanism of the beneficial action of taurine on marine teleost fish, red sea bream (Pagrus major), by analyzing the hepatic metabolism. Moreover, the ameliorative effects of the nutrient against cadmium toxicity and bioaccumulation were further evaluated. The fish were fed a di...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-016-0274-7
更新日期:2017-02-01 00:00:00
abstract::Endocrine-disrupting chemicals (EDCs) can affect normal sexual differentiation in fish. Foxl2, one forkhead transcription factor, plays an important role in ovarian differentiation in the early development of the female gonad in mammals and fish. How EDCs affect Foxl2 expression is little known. In this study, we isol...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-011-9548-2
更新日期:2012-06-01 00:00:00
abstract::The present study develops an experimental procedure aimed to estimate the efficiency of protein digestion in fish by measuring both gut transit rate and total amount of the main intestinal proteases (trypsin and chymotrypsin). The selected species was the Nile tilapia (Oreochromis niloticus). Total time for digestion...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-010-9385-8
更新日期:2010-12-01 00:00:00
abstract::This study aimed to investigate the stress response of Sparus aurata specimens fed with nutraceutical aquafeed brewer's yeast (Saccharomyces cerevisiae) and spirulina (Arthrospira platensis). For that purpose, 96 (169.0 ± 2.8 g) animals were distributed randomly in 12 tanks (8 fish per tank, 4 replicates) and divided ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00677-8
更新日期:2019-12-01 00:00:00
abstract::Juvenile sea bass, 1.5 years old, of mixed sex, held on long photoperiods were fed early on the photoperiod and late on the photoperiod, using different diets. Fish fed natural diets showed a daily rhythmicity of plasma insulin, liver c-AMP, plasma glucose, liver glycogen and muscle glycogen content, however, fish fed...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01874796
更新日期:1988-10-01 00:00:00
abstract::The trace element composition of the fish otolith is an indicator of biomineralization. In contrast to other skeletal tissue, the otolith retains its entire original structure and does not absorb any elements after the fish dies. Because otoliths in carp degrade very slowly in the dead body, the information it provide...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-008-9294-2
更新日期:2010-03-01 00:00:00
abstract::Levels of reproductively-related steroids were determined in captive male sand tiger sharks, Carcharias taurus, maintained at two institutions: SeaWorld Adventure Park Orlando and the National Aquarium in Baltimore. Sexual conflicts were absent at the former, but were documented at the latter. Serum titers of 17beta-e...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-008-9202-9
更新日期:2008-12-01 00:00:00
abstract::The serum carotenoid level gradually increased during the spawning migration of chum salmon (Oncorhynchus keta). Zeaxanthin, a yellowish carotenoid, was detected in the serum of spent males, while astaxanthin was a main carotenoid in the serum of males and females at other developmental stages. A high bilirubin level ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01875644
更新日期:1988-05-01 00:00:00
abstract::Evidence has recently been presented for variation in the inducibility of various 7-alkoxycoumarin-O-dealkylase activities in liver microsomes from a number of mammalian species by β-naphthoflavone (βNF). In the present study we have investigated the inducibility of hepatic microsomal 7-methoxycoumarin-O-demethylase, ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01875022
更新日期:1989-07-01 00:00:00
abstract::Environmental stressors caused by inadequate aquaculture management strategies suppress the immune response of fish and make them more susceptible to diseases. Therefore, efforts have been made to relieve stress in fish by using various functional feed additives in the diet, including probiotics. The present work eval...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-016-0277-4
更新日期:2017-02-01 00:00:00
abstract::The purpose of the current study was to examine seasonal changes in seawater tolerance and growth performance of anadromous Arctic charr (Salvelinus alpinus L.) held at the same temperature (8°C) during winter and summer. Charr (20-27 cm), previously reared in freshwater under natural photoperiod, were transferred eit...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004413
更新日期:1993-12-01 00:00:00
abstract::Fish red blood cells (RBCs) exhibit an oxygen-dependent regulatory volume decrease (RVD) in hypoosmotic environment. In higher vertebrates, membrane-associated hemoglobin is involved in the regulation of osmotic ion movements across the cellular membrane. However, whether the hemoglobin conformational state plays a ro...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00689-4
更新日期:2019-12-01 00:00:00
abstract::The development of digestive enzymes during the early ontogeny of the Mayan cichlid (Cichlasoma urophthalmus) was studied using biochemical and electrophoretic techniques. From yolk absorption (6 days after hatching: dah), larvae were fed Artemia nauplii until 15 dah, afterward they were fed with commercial microparti...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-010-9431-6
更新日期:2011-03-01 00:00:00
abstract::The present study was conducted to investigate the effects of high dietary lipid levels on growth, metabolism, antioxidant capacity, and immune responses of largemouth bass. Fish (initial body weight 13.38 ± 0.11 g) were fed three isonitrogenous semi-purified diets containing 5%, 10%, and 20% lipid, respectively. The ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00705-7
更新日期:2020-02-01 00:00:00
abstract::The sperm duct epithelium of brook trout (Salvelinus fontinalis), mountedin vitro in Ussing-style epithelial chambers actively absorbs Na(+) (measured as the short-circuit current, Isc) and secretes K(+) (measured using(86)Rb(+) as tracer). Dibutyryl-cyclic-adenosine monophosphate (db-cAMP) and 3-isobutyl-1-methylxant...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004725
更新日期:1989-06-01 00:00:00
abstract::This study was conducted to determine if diets supplemented with turmeric powder (Curcuma longa) affected the reversible sterilization of Pseudotropheus socolofi. Three experimental diets were formulated to contain 0%, 10%, and 14% turmeric powder. The fish (mean weight 13 g) were randomly divided into groups consisti...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-020-00781-0
更新日期:2020-08-01 00:00:00
abstract::Transferrin mRNA in liver was quantified in farmed Atlantic salmon (Salmo salar) fed three different levels (310-, 65-, and 40 ppm) of dietary iron for 26 weeks. At the time of sampling, the fish fed 40 ppm iron showed symptoms of iron deficiency. Mean values of liver storage iron was reduced from 84 to 12 mg kg(-1) a...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF02112358
更新日期:1996-08-01 00:00:00
abstract::Three consecutive days of injections of triiodothyronine (T3)(0.038, 0.075, 0.15 and 1.54 nmoles/g) significantly elevated the acetylcholinesterase (AchE) activity in the brain of Singi fish, Heteropneustes fossilis (Bloch). The higher doses of 0.075, 0.15 and 1.54 nmoles of T3/g induced a greater increase in enzyme a...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004508
更新日期:1993-03-01 00:00:00
abstract::Measurable in vitro hepatic monodeiodinase activity of the southern hemisphere lamprey, Geotria australis, was present only during the first 5 of the 16 month upstream spawning migration of this species. The production of T3 from T4 in vitro was pH-sensitive, and exhibited typical Michaelis-Menton kinetics. No consist...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00003372
更新日期:1990-09-01 00:00:00
abstract::Muscle tissues contribute 34-48 % of the total body mass in fish. Proteomic analysis enables better understanding of the skeletal muscle physiology and metabolism. A proteome map reflects the general fingerprinting of the fish species and has the potential to identify novel proteins which could serve as biomarkers for...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0046-9
更新日期:2015-06-01 00:00:00
abstract::The antioxidative role of cerium was investigated in the liver of silver crucian carp injected with lead. The fish were intraperitoneally injected with 10, 20, or 30 mg/kg wet weight PbCl(2). After a 14-day period of incubation, 35 animals were injected with a solution of 1.5 mg/kg wet weight CeCl(3). After 42 days, t...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-008-9301-7
更新日期:2010-09-01 00:00:00
abstract::The manner in which behavior influences the gonadotropin-releasing hormone (GnRH) axis in hermaphroditic fishes is not understood. The Gilthead seabream, Sparus aurata, is a protandrous hermaphrodite with a complex gonadal cycle consisting of a quiescent, pre-spawning, spawning, and post-spawning stage. On two separat...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-012-9757-3
更新日期:2013-08-01 00:00:00
abstract::Cytochrome P450 aromatase, which is encoded by the CYP19a gene, converts androgens to estradiol. Considerable evidence suggests that estrogens play an important role in fish reproductive process. Therefore CYP19a is an excellent candidate gene for reproductive traits. Variants in the promoter of the CYP19a gene might ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-008-9237-y
更新日期:2009-08-01 00:00:00
abstract::The present study made an attempt to measure the cortisol content, as an indicator of stress response, in rainbow trout embryos which were exposed to different densities and handling stress (air exposure) during incubation. The three densities of experimental embryos at early development stages were considered as 2.55...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-014-9999-3
更新日期:2015-04-01 00:00:00
abstract::Enzymatic changes that occur in the white somatic muscle of rainbow trout (Oncorhynchus mykiss) in response to spawning were investigated, and the evenness of their distribution across the ventral-dorsal plane of this muscle was assessed. Four enzymes that are involved in energy metabolism were measured (phosphofructo...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004344
更新日期:1995-12-01 00:00:00