Control of ion transport by the sperm duct epithelium of brook trout (Salvelinus fontinalis).

Abstract:

:The sperm duct epithelium of brook trout (Salvelinus fontinalis), mountedin vitro in Ussing-style epithelial chambers actively absorbs Na(+) (measured as the short-circuit current, Isc) and secretes K(+) (measured using(86)Rb(+) as tracer). Dibutyryl-cyclic-adenosine monophosphate (db-cAMP) and 3-isobutyl-1-methylxanthine (IMX) produce a rapid, sustained stimulation of both ion transport processes, but the hormone connected to the response is unknown. Purified sockeye salmon CON A2 gonadotropin (GtH) produces a dose-dependent, rapid and sustained rise in Na(+) uptake and K(+) secretion. The time course, electrophysiological and transport characteristics are similar to those evoked by IMX. Carbohydrate-poor (chum salmon CON A1) GtH is ineffective. Pretreatment of fish with 17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-P) significantly increases milt volume but is without effect on resting or stimulated (IMX + db-cAMP) levels of sperm duct ion transport. This is the first indication of a direct, rapid action of GtH on ion transport by the vertebrate blood-testis barrier. The results suggest direct involvement of GtH in control of later stages of sperm maturation.

journal_name

Fish Physiol Biochem

authors

Marshall WS,Bryson SE,Idler DR

doi

10.1007/BF00004725

subject

Has Abstract

pub_date

1989-06-01 00:00:00

pages

331-6

issue

1-6

eissn

0920-1742

issn

1573-5168

journal_volume

7

pub_type

杂志文章