Abstract:
:Blood being a vehicle for the transport of industrial pollutants in living system, fish hematology is considered as potent biomarker. In the present study, we investigated respective sublethal effects of pulp and paper mill effluents on hematology of two commonly cultured carps, Cyprinus carpio and Ctenopharyngodon idella, using optical, scanning electron microscopy and energy-dispersive X-ray spectroscopy. Irrespective of species, results showed significant decrease in erythrocyte, hematocrit and hemoglobin contents while an increase in white blood cell counts (P < 0.05). We observed an increasing trend of MCV (170.0 ± 3.07 to 193.16 ± 2.5) and MCH (34.31 ± 1.89 to 38.71 ± 3.61) up to 28th day in C. carpio (P < 0.05), while, in C. idella, the highest percent increase in MCV (180.8 ± 2.19) and MCH (32.9 ± 0.62) was observed on seventh exposure day, which subsequently declined, respectively, to 173.1 ± 17.1 and 27.9 ± 2.45 on 28th day. Unlike C. carpio, significant and progressive MCHC declining trend (18.23 ± 0.28 to 16.13 ± 0.31) was observed in C. idella. The most commonly observed abnormalities under SEM include echinocytes, cytoplasmic blebbing, cytoplasmic ring, spherocytes, lobopodial projections and acanthocytes in red blood cells of exposed fishes. EDS further revealed the presence of aluminum, antimony, arsenic, cadmium, mercury, tungsten, zinc and titanium; some of these metals were not even detected in the effluent samples, suggesting the probable metal bio-concentration in fish tissue, and subsequent jeopardization is a major concern particularly in the industrial area. Our study further suggested the use of sensitive and specific techniques like SEM and EDS in fish hematological biomarker analysis along with the conventional approach.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Dey S,Choudhury MD,Das Sdoi
10.1007/s10695-016-0258-7subject
Has Abstractpub_date
2016-12-01 00:00:00pages
1791-1805issue
6eissn
0920-1742issn
1573-5168pii
10.1007/s10695-016-0258-7journal_volume
42pub_type
杂志文章abstract::The mudskipperB. boddaerti, was able to survive in waters of intermediate salinities (4-27‰). Fish submerged in dechlorinated tap water suffered 60% mortality by the fifth day while 60% of those in 100% sea-water (sw) died after the third day of exposure. After being submerged in 50% or 80% sw for 7 days, the plasma o...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01987612
更新日期:1991-03-01 00:00:00
abstract::Annual plasma insulin and glucose cycles were studied inDicentrarchus labrax maintained in either seawater (37.8‰) or brackish water (3.5‰). In both media, the highest insulin levels were found during the prespawning period (August-November) coincident to increases in weight and a decrease in plasma glucose. During sp...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF02110880
更新日期:1987-10-01 00:00:00
abstract::The pharmacokinetic properties of sarafloxacin were investigated after single intravenous (i.v.) and oral (p.o.) administration of 10 mg/kg body weight (b.w.) in allogynogenetic silver crucian carp at 24-26 °C. The plasma concentrations of sarafloxacin were determined by high-performance liquid chromatography. After i...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0141-y
更新日期:2016-02-01 00:00:00
abstract::An immunohistochemical study of the sturgeon (Acipenser baeri) pituitary was undertaken using antisera directed against hormones from various classes of vertebrates, including the only pituitary hormone available from sturgeon, gonadotrophin. A positive reaction was obtained after application of antisera towards the f...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01875699
更新日期:1988-07-01 00:00:00
abstract::Ubiquitin-conjugating enzymes (E2s) play an important role in the mechanism of ubiquitin transfer. Although in most species many of these enzymes share high sequence and structural conservation, their existence and functions in the lamprey remain unknown. In this study, we identified and characterized a ubiquitin-conj...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0139-5
更新日期:2016-02-01 00:00:00
abstract::In addition to catalytical assays, immunochemical techniques have recently been employed to measure induction of the cytochrome P-450 (P450) monooxygenase system in fish with polyaromatic hydrocarbons (PAH). In the present study, polyclonal antibodies were raised against rainbow trout P450IA1. Levels of rainbow trout ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF02265140
更新日期:1991-06-01 00:00:00
abstract::Autophagy is a cellular process which occurs in eukaryotic cells. To study the mechanism regulating polyploid fish growth and development is of significance in genetic, because of its growth advantages and economic values. This study focused on triploid female rainbow trout (RBT) which discusses the effects of autopha...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-017-0423-7
更新日期:2018-02-01 00:00:00
abstract::The present study explored the protective role of dietary the extract of Angelica sinensis (EAs) on high density, CuSO4, or trichlorfon-treated Crucian carp (Carassius auratus auratus). Firstly, the study showed that the optimum density for growth and growth inhibition was 0.49 and 0.98 fish L-1 water, respectively. D...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-020-00821-9
更新日期:2020-10-01 00:00:00
abstract::Oxidation of rainbow trout haemoglobin (Hb) by nitrite proceeded via an initial lag phase followed by autocatalysis when the O2 saturation of the Hb was high. At low O2 saturations, the rate of methaemoglobin (metHb) formation was strongly reduced and autocatalysis was absent. Addition of adenosine triphosphate and gu...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004376
更新日期:1993-08-01 00:00:00
abstract::The questions addressed in this study were: 1) whether insulin added to the incubation medium can down-regulate (125)I insulin binding to isolated hepatocytes of Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss); 2) whether quantitative assessment of insulin processing can be made on isolated fish ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004590
更新日期:1993-07-01 00:00:00
abstract::A 2 × 2 × 2 factorial experiment was conducted to delineate the main effect of water soaking of plant ingredients, phytase, cellulase, and their interactions on the growth and digestive enzyme activities of Labeo rohita fingerlings. Two basal diets were prepared using water-soaked (S) or unsoaked (US) plant-based ingr...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-011-9511-2
更新日期:2012-04-01 00:00:00
abstract::Histo- and immunohistochemical techniques have recently been used to study the fibre type and myosin expression in fish muscle during development. In the present work, embryonic, larval and adult myosin isozymes (heavy and light chains) and parvalbumin isotypes were analyzed, from fertization to the adult stage, by po...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004524
更新日期:1992-08-01 00:00:00
abstract::Muscle accretion is affected by the difference between protein synthesis and its degradation. Studies on different species revealed that muscle proteolysis is mediated by different pathways including the ubiquitin-proteasome pathway in which the ubiquitin protein ligases play an important role. These muscle atrophy as...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00667-w
更新日期:2019-08-01 00:00:00
abstract::Three cytosolic glutathione S-transferases [E.C. 2.5.1.18] were identified in liver of a marine flatfish, the plaice (Pleuronectes platessa) and the two major isoforms were purified to homogeneity. There was no evidence for the presence of basic transferases. The plaice GST's exhibited comparable hematin binding to ra...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00003400
更新日期:1990-11-01 00:00:00
abstract::To determine if there were organ-specific changes in immune responses or immune-endocrine interaction, we monitored in vitro immune response, cortisol sensitivity and number and affinity of glucocorticoid receptors (GR) in leukocytes from freshwater-adapted juvenile coho salmon (Oncorhynchus kisutch) during the physio...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004605
更新日期:1993-04-01 00:00:00
abstract::Previous study from our laboratory on thiourea-induced thyroid hormone depletion in mature male catfish demonstrated that thyroid hormones play a significant role in testicular function. In the present study, we aimed to analyze the changes in the expression pattern of several steroidogenic enzyme genes after thyroid ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-006-0036-z
更新日期:2005-04-01 00:00:00
abstract::Spermiation and changes in androgen (testosterone, T and 11-ketotestosterone, 11-KT) levels were studied in sterlet (Acipenser ruthenus) treated with GnRH agonist implants (DAla(6)-Pro(9)-LHRHa) at 25 and 75 μg kg(-1) b.w. and compared with those males treated with 4 mg kg(-1) b.w. of carp pituitary extract (CPE) and ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-012-9662-9
更新日期:2012-12-01 00:00:00
abstract::Exposure to high temperatures can lead to thermotolerance in fish, which is hypothesized to potentially improve post-release survival in species under restocking programs, like Atlantic sturgeon. The aim of this study was to determine whether Atlantic sturgeon juveniles exposed to a 4-week temperature treatment respon...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-020-00818-4
更新日期:2020-10-01 00:00:00
abstract::Using a tame animal, the impact of otter (Lutra lutra) disturbance on over-wintering carp (Cyprinus carpio) was monitored in two experiments, 133 and 140 days, respectively, over two consecutive winters (November-April). The level of stress in over-wintering carp exposed to various intensities of disturbance by otters...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-007-9180-3
更新日期:2008-09-01 00:00:00
abstract::This paper presents a comparative study of the roles of Cl- and HCO3- in the functioning of the GABAAR-associated Cl-/HCO3--ATPase of the plasma membranes of the olfactory sensory neurons (OSNs) and mature brain neurons (MBNs) of fish. The ATPase activity of OSNs and its dephosphorylation were increased twofold by Cl-...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-017-0455-z
更新日期:2018-04-01 00:00:00
abstract::The impacts of bacterial infection on cultivated fish species, African catfish, were investigated using oxidative stress biomarkers [lipid peroxidation (LPO) and protein carbonylation] and the activities of important antioxidant/detoxifying enzymes [catalase and glutathione S-transferase (GST)]. Fish were inoculated v...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-013-9847-x
更新日期:2014-04-01 00:00:00
abstract::The aim of the present study was to explore the effect of waterborne zinc (control, 0.85, 2.20, 3.10 mg/l, respectively) exposure on lipid deposition and metabolism in the hepatopancreas and muscle of grass carp Ctenopharyngodon idella. The lipid content, Zn accumulation, and the activities and expression levels of se...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-016-0200-z
更新日期:2016-08-01 00:00:00
abstract::Three consecutive days of injections of triiodothyronine (T3)(0.038, 0.075, 0.15 and 1.54 nmoles/g) significantly elevated the acetylcholinesterase (AchE) activity in the brain of Singi fish, Heteropneustes fossilis (Bloch). The higher doses of 0.075, 0.15 and 1.54 nmoles of T3/g induced a greater increase in enzyme a...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004508
更新日期:1993-03-01 00:00:00
abstract::This study was conducted to determine if diets supplemented with turmeric powder (Curcuma longa) affected the reversible sterilization of Pseudotropheus socolofi. Three experimental diets were formulated to contain 0%, 10%, and 14% turmeric powder. The fish (mean weight 13 g) were randomly divided into groups consisti...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-020-00781-0
更新日期:2020-08-01 00:00:00
abstract::Enzymatic changes that occur in the white somatic muscle of rainbow trout (Oncorhynchus mykiss) in response to spawning were investigated, and the evenness of their distribution across the ventral-dorsal plane of this muscle was assessed. Four enzymes that are involved in energy metabolism were measured (phosphofructo...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004344
更新日期:1995-12-01 00:00:00
abstract::Changes in tissue and plasma isoenzymes of alkaline phosphatase (ALP) were qualitatively and quantitatively determined for male and female Atlantic salmon parr, silvery parr, smolt, immature grilse, prespawning grilse and postspawning grilse using cellulose acetate electrophoresis, densitometry and spectrophotometry. ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004451
更新日期:1994-03-01 00:00:00
abstract::The large yellow croaker (Larimichthys crocea) is a marine fish that is economically important to Chinese fisheries, and its reproductive and developmental biology have been extensively investigated. However, the molecular mechanism of oogenesis in L. crocea is not clear. Here, we investigated the multiple vitellogeni...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-018-0569-y
更新日期:2019-06-01 00:00:00
abstract::Aryl hydrocarbon receptor (AhR) agonists are known to cause lethal cardiovascular deformities in fish after developmental exposure. Acute adult fish toxicity of AhR agonists is thought to be minimal, but limited evidence suggests sublethal effects may also involve the cardiac system in fish. In the present study, adul...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-014-9982-z
更新日期:2015-02-01 00:00:00
abstract::The adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL)-mediated lipolysis play important roles in lipid catabolism. ATGL is considered the central rate-limiting enzyme in the mobilization of fatty acids in mammals. Currently, severe fat accumulation has been commonly detected in farmed fish globally...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-020-00904-7
更新日期:2020-11-27 00:00:00
abstract::The development of digestive enzymes during the early ontogeny of the Mayan cichlid (Cichlasoma urophthalmus) was studied using biochemical and electrophoretic techniques. From yolk absorption (6 days after hatching: dah), larvae were fed Artemia nauplii until 15 dah, afterward they were fed with commercial microparti...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-010-9431-6
更新日期:2011-03-01 00:00:00