Isolation of pituitary glycoprotein gonadotropins from the grass carp (Ctenopharyngodon idell).

Abstract:

:Gonadotropins (GTH) were isolated from pituitary glands of grass carp and their properties investigated. Acetone-dried pituitaries were extracted with ammonium acetate-ethanol and were then passed through cation exchanger, anion exchanger, and gel filtration column. Two glycoprotein gonadotropins (DE I and DE II) were isolated of MW 56,000 and 53,000 daltons, respectively, as determined by gel filtration technique. Isoelectric points of the two GTHs also differed from each other. Sialic acid contents were 3.64% and 1.47% for DE I and DE II, respectively. Both GTH's stimulated, in a similar manner, testicular androgen and ovarian estradiol synthesisin vitro.

journal_name

Fish Physiol Biochem

authors

Yu JY,Shen ST

doi

10.1007/BF00004705

subject

Has Abstract

pub_date

1989-06-01 00:00:00

pages

177-83

issue

1-6

eissn

0920-1742

issn

1573-5168

journal_volume

7

pub_type

杂志文章