Abstract：
:Two-dimensional gel electrophoresis (2-DE), matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) mass spectrometry, and database searching were used to analyze the effects of triploidization heat shock treatment on protein expression in rainbow trout eyed embryo and fry. After fertilization, the eggs were incubated at 10 °C for 10 min. Half of the eggs were then subjected to heat shock for 10 min submerged in a 28 °C water bath to induce triploidy. The remainder was incubated normally and used as diploid controls. Specimens of eyed embryos and fry were taken on 18 and 76 days post-fertilization, respectively. In the eyed embryo extracts, seven protein spots were significantly changed in abundance between the control and heat-shocked groups and one of these was decreased while the others were increased in the heat shock-treated group. Of the spots that were shown to change in abundance in the eyed embryos with heat shock treatment, two were identified as vitellogenin, while the others were creatine kinase and angiotensin I. In the 2-DE from the fry muscle extraction, 23 spots were significantly changed in abundance between the diploid and triploid groups. Nineteen of these showed a decreased abundance in diploids, while the remaining four spots had an increased abundance. Triploidization caused differential expression of muscle metabolic proteins including triosephosphate isomerase, glyceraldehyde-3-phosphate dehydrogenase, and beta-enolase. Myosin heavy chain as a structural protein was also found to change in abundance in triploids. The altered expression of both structural and metabolic proteins in triploids was consistent with their increased cell size and lower growth performance.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Babaheydari SB,Keyvanshokooh S,Dorafshan S,Johari SAdoi
10.1007/s10695-016-0312-5subject
Has Abstractpub_date
2017-04-01 00:00:00pages
579-589issue
2eissn
0920-1742issn
1573-5168pii
10.1007/s10695-016-0312-5journal_volume
43pub_type
杂志文章abstract：:Oxidation of rainbow trout haemoglobin (Hb) by nitrite proceeded via an initial lag phase followed by autocatalysis when the O2 saturation of the Hb was high. At low O2 saturations, the rate of methaemoglobin (metHb) formation was strongly reduced and autocatalysis was absent. Addition of adenosine triphosphate and gu...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004376
更新日期：1993-08-01 00:00:00
abstract：:A study was conducted to investigate the effect of dietary yeast polysaccharides on some hematologic parameters and intestinal morphology of channel catfish. Channel catfish were fed diets containing yeast polysaccharides at 0 (control), 0.1, 0.2, or 0.3 % for 7 weeks. Each diet was provided to 10 channel catfish spec...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10695-012-9631-3
更新日期：2012-10-01 00:00:00
abstract：:For rainbow trout Oncorhynchus mykiss, high temperature is a major abiotic stress that limits its growth and productivity. In this study, spleen macrophage respiratory burst (RB), serum superoxide dismutase (SOD), serum malondialdehyde (MDA) and mRNA expression of the SERPINH1 (HSP47) gene in different tissues (liver,...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0170-6
更新日期：2016-04-01 00:00:00
abstract：:Plasma chemistry, lipid metabolism and vitellogenin gene expression of captive Sterlet sturgeon Acipenser ruthenus were studied in different maturity stages. A total of 32 fish were sampled, and maturity stages were identified on the basis of histological criteria and direct observation. Females were classified to fou...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0189-8
更新日期：2016-06-01 00:00:00
abstract：:The trace element composition of the fish otolith is an indicator of biomineralization. In contrast to other skeletal tissue, the otolith retains its entire original structure and does not absorb any elements after the fish dies. Because otoliths in carp degrade very slowly in the dead body, the information it provide...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-008-9294-2
更新日期：2010-03-01 00:00:00
abstract：:In order to specify the timing of some changes in ovarian steroid production during the transition from vitellogenesis to ovulation, plasma hormones levels andin vivo andin vitro responses of the ovary to salmon gonadotropin (s-GtH) or dibutyryl-cyclic adenosine mono-phosphate (db-cAMP) were recorded in relationship w...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF02264076
更新日期：1986-10-01 00:00:00
abstract：:We have developed a novel single cell real-time quantitative PCR technique, which incorporates harvesting marker-identified single cells using laser-capture. Here, for the first time in a vertebrate species, using this innovative single cell gene profiling technique, we report the presence of G-protein coupled recepto...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-006-0018-1
更新日期：2005-04-01 00:00:00
abstract：:The rate of oxygen consumption of minced whole body was determined volumetrically, as an indication of metabolic rate in vitro (M in vitro ), at 20°C in porgy Pagrus major ranging from 0.0002 g (just after hatch) to 2.9 g (67 days old) in body mass. A triphasic relationship was found between M in vitro of individual f...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004511
更新日期：1992-10-01 00:00:00
abstract：:Sperm quality parameters in rainbow trout (Oncorhynchus mykiss) were investigated during normal season spawning (November-January) and out-season spawning (July-August) treated with artificial photoperiod manipulation. Normal spawning males (n = 15) were kept in an open concrete pond under natural condition. Out-seaso...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-018-0564-3
更新日期：2018-12-01 00:00:00
abstract：:The Brazilian freshwater fish diversity is the richest in the world. Only 0.7% of all Brazilian species have had any aspect of their sperm biology addressed up to this date. The majority of the fish species described in this review migrate during the spawning season (a phenomenon known as piracema). Urbanization, poll...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章,评审
doi：10.1007/s10695-008-9240-3
更新日期：2009-03-01 00:00:00
abstract：:The aims of the present study were to characterize sperm quality and to quantify seasonal changes in sexual hormone (testosterone [T], 11-ketotestosterone [11-KT] and 17,20β-dihydroxypregn-4-en-3-one [17,20β-P]) levels in male brill (Scophthalmus rhombus) plasma, as well as to test a more intensive sampling strategy t...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-012-9692-3
更新日期：2013-04-01 00:00:00
abstract：:Connective tissue growth factor (ctgf) is involved in the proliferation, migration, adhesion of cell, and the constituent of extracellular matrix, which plays an important role in embryogenesis, angiogenesis, wound repair, and fibrosis diseases. In this study, the cDNA sequence of grass carp ctgf gene was cloned by ra...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00653-2
更新日期：2019-08-01 00:00:00
abstract：:In this study, for the first time, both neuropeptides isotocin (IT) and arginine vasotocin (AVT) have been identified and measured in urophysis, the neurohaemal organ of the caudal neurosecretory system of teleost fish. So far, AVT, but not IT, was quantified by radioimmunoassay (RIA) in urophysis of several fish spec...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-012-9746-6
更新日期：2013-08-01 00:00:00
abstract：:Bacillus circulans PB7, isolated from the intestine of Catla catla, was evaluated for use as a probiotic supplement in the feeds for the fingerlings of Catla catla. The effect of supplement on the growth performance, feed utilization efficiency, and immune response was evaluated. Catla fingerlings (ave. wt. 6.48 +/- 0...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-008-9272-8
更新日期：2009-08-01 00:00:00
abstract：:Muscle accretion is affected by the difference between protein synthesis and its degradation. Studies on different species revealed that muscle proteolysis is mediated by different pathways including the ubiquitin-proteasome pathway in which the ubiquitin protein ligases play an important role. These muscle atrophy as...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00667-w
更新日期：2019-08-01 00:00:00
abstract：:Plasma and liver composition, liver enzyme activities, and metabolite flux in isolated hepatocytes have been studied in immature American eels,Anguilla rostrata, injected daily IP with saline or cortisol (0.35 mg/kg eel). Plasma cortisol values were significantly increased above saline controls in those eels receiving...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF02290211
更新日期：1986-05-01 00:00:00
abstract：:Gonadotropins (GTH) were isolated from pituitary glands of grass carp and their properties investigated. Acetone-dried pituitaries were extracted with ammonium acetate-ethanol and were then passed through cation exchanger, anion exchanger, and gel filtration column. Two glycoprotein gonadotropins (DE I and DE II) were...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004705
更新日期：1989-06-01 00:00:00
abstract：:Prolactin (PRL) is one of the most versatile hormones found in the pituitary of vertebrates and exerts its actions through binding to a specific PRL receptor (PRLR). Here we describe the cloning and characterization of a second prolactin receptor (ntPRLR2), isolated from the ovary of Nile tilapia (Oreochromis niloticu...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-009-9355-1
更新日期：2010-06-01 00:00:00
abstract：:This study describes the digestible protein (DP) and digestible energy (DE) utilization in juvenile mulloway, and determined the requirements for maintenance. This was achieved by feeding triplicate groups of fish weighing 40 or 129 g held at two temperatures (20 or 26 degrees C), on a commercial diet (21.4 g DP mJ DE...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-008-9296-0
更新日期：2010-03-01 00:00:00
abstract：:A completely randomized experimental design carried out to investigate the effects of different levels of Pediococcus acidilactici (PA) including 0 (basal diet as a control diet), 1 × 106, 2 × 106, 4 × 106, and 8 × 106 colony-forming unit (CFU) per gram of the diet for 60 days on the mucosal immunity responses, growth...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-020-00903-8
更新日期：2020-11-26 00:00:00
abstract：:Endocrine-disrupting chemicals (EDCs) can affect normal sexual differentiation in fish. Foxl2, one forkhead transcription factor, plays an important role in ovarian differentiation in the early development of the female gonad in mammals and fish. How EDCs affect Foxl2 expression is little known. In this study, we isol...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9548-2
更新日期：2012-06-01 00:00:00
abstract：:This study aimed to investigate the stress response of Sparus aurata specimens fed with nutraceutical aquafeed brewer's yeast (Saccharomyces cerevisiae) and spirulina (Arthrospira platensis). For that purpose, 96 (169.0 ± 2.8 g) animals were distributed randomly in 12 tanks (8 fish per tank, 4 replicates) and divided ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00677-8
更新日期：2019-12-01 00:00:00
abstract：:The stress response transmitted by the HPA axis is one of the best examples of neuroendocrine-immune interactions that are critical for survival. Analogous to the situation in mammals, the stress response in fish is characterized by the activation of the hypothalamo-pituitary-interrenal axis (HPI). Effects of cortisol...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0084-3
更新日期：2015-10-01 00:00:00
abstract：:In the end of the Discussion section, following information should be included. ...
journal_title：Fish physiology and biochemistry
pub_type： 已发布勘误
doi：10.1007/s10695-020-00889-3
更新日期：2020-12-01 00:00:00
abstract：:The present study aims to investigate the effects of both garlic and curcumin through evaluating their therapeutic properties as antioxidants on liver and kidney functions, hepatic antioxidants and GPx gene expression against aflatoxicosis of O. niloticus. In total, 180 of tilapia were divided into ten groups; T1 repr...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0164-4
更新日期：2016-04-01 00:00:00
abstract：:In most sciaenids, males possess sonic muscles and produce sound through the contraction of these muscles and amplification of the swim bladder. The sonic muscles in some fishes exhibit seasonal changes in size. For example, they are hypertrophic in the spawning season, and atrophic in the non-spawning months. The pro...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9502-3
更新日期：2011-12-01 00:00:00
abstract：:In aquaculture, antibiotics are the traditional treatment used against bacterial infections. However, their use has increasingly come into question given their effects on fish and, possibly, on human health. Consequently, there is interest in developing alternative treatments aimed at stimulating the innate immune res...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0155-5
更新日期：2016-04-01 00:00:00
abstract：:The pineal organ of fish, through its 24h rhythmic release of melatonin, acts as a transducer of the photoperiod, influencing different physiological functions (e.g., reproduction, growth). The target sites for melatonin are poorly known in fish, especially marine species. A radioligand study was undertaken using the ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01875583
更新日期：1996-11-01 00:00:00
abstract：:Zinc pyrithione (ZPT) is a broad-spectrum antibacterial and antifungal agent; therefore, it is widely used in industry and civilian life. It is discharged into the aquatic environment with industrial and civilian waste water. Carassius sp. is one of the most widely distributed and farmed fish in China. The effects of ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-016-0262-y
更新日期：2017-02-01 00:00:00
abstract：:Animal and/or plant protein sources substituting fishmeal in the diets keep being developed due to its high price. The purpose of this study is to determine response of dietary substitution of fishmeal with silkworm pupae meal, promate meal®, meat and bone meal and/or their combination on the performance of juvenile o...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9555-3
更新日期：2012-06-01 00:00:00
