Purification and characterization of pepsinogens and pepsins from the stomach of rice field eel (Monopterus albus Zuiew).


:Three pepsinogens (PG1, PG2, and PG3) were highly purified from the stomach of freshwater fish rice field eel (Monopterus albus Zuiew) by ammonium sulfate fractionation and chromatographies on DEAE-Sephacel, Sephacryl S-200 HR. The molecular masses of the three purified PGs were all estimated as 36 kDa using SDS-PAGE. Two-dimensional gel electrophoresis (2D-PAGE) showed that pI values of the three PGs were 5.1, 4.8, and 4.6, respectively. All the PGs converted into corresponding pepsins quickly at pH 2.0, and their activities could be specifically inhibited by aspartic proteinase inhibitor pepstatin A. Optimum pH and temperature of the enzymes for hydrolyzing hemoglobin were 3.0-3.5 and 40-45 °C. The K (m) values of them were 1.2 × 10⁻⁴ M, 8.7 × 10⁻⁵ M, and 6.9 × 10⁻⁵ M, respectively. The turnover numbers (k(cat)) of them were 23.2, 24.0, and 42.6 s⁻¹. Purified pepsins were effective in the degradation of fish muscular proteins, suggesting their digestive functions physiologically.


Fish Physiol Biochem


Weng WY,Wu T,Chen WQ,Liu GM,Osatomi K,Su WJ,Cao MJ




Has Abstract


2011-09-01 00:00:00












  • Presence and ontogeny of intestinal and pancreatic phospholipase A2-like proteins in the Red Sea bream,Pagrus major. An immunocytochemical study.

    abstract::We have studied the location and the ontogeny of the digestive enzyme, phospholipase A2 (PLA2) immunohistochemically in the adult and larvae/juvenile of the red sea breamPagrus major by using an antiserum against theNaja naja venom PLA2. The antiserum reacts with at least one enzyme among the PLA2s purified from the f...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Uematsu K,Kitano M,Morita M,Iijima N

    更新日期:1992-02-01 00:00:00

  • Seasonal and estradiol-17β-stimulated changes in thyroid function of adult Geotria australis, a southern hemisphere lamprey.

    abstract::Measurable in vitro hepatic monodeiodinase activity of the southern hemisphere lamprey, Geotria australis, was present only during the first 5 of the 16 month upstream spawning migration of this species. The production of T3 from T4 in vitro was pH-sensitive, and exhibited typical Michaelis-Menton kinetics. No consist...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Leatherland JF,Macey DJ,Hilliard RW,Leatherland A,Potter IC

    更新日期:1990-09-01 00:00:00

  • Serum thyroxine, estradiol-17β, and testosterone profiles during the parr-smolt transformation of masu salmon, Oncorhynchus masou.

    abstract::Changes in serum thyroxine (T4), estradiol-17β (E2) and testosterone (T) levels during the parr-smolt transformation (smoltification) were investigated in the masu salmon (Oncorhynchus masou) in 1985 and 1987. T4 showed a peak in levels at the early stage of smoltification and E2 and T levels peaked almost at the same...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Yamada H,Ohta H,Yamauchi K

    更新日期:1993-07-01 00:00:00

  • Proteomic profiling of white muscle from freshwater catfish Rita rita.

    abstract::Muscle tissues contribute 34-48 % of the total body mass in fish. Proteomic analysis enables better understanding of the skeletal muscle physiology and metabolism. A proteome map reflects the general fingerprinting of the fish species and has the potential to identify novel proteins which could serve as biomarkers for...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Mohanty BP,Mitra T,Banerjee S,Bhattacharjee S,Mahanty A,Ganguly S,Purohit GK,Karunakaran D,Mohanty S

    更新日期:2015-06-01 00:00:00

  • Integrated biomarker parameters response to the toxic effects of high stocking density, CuSO4, and trichlorfon on fish and protective role mediated by Angelica sinensis extract.

    abstract::The present study explored the protective role of dietary the extract of Angelica sinensis (EAs) on high density, CuSO4, or trichlorfon-treated Crucian carp (Carassius auratus auratus). Firstly, the study showed that the optimum density for growth and growth inhibition was 0.49 and 0.98 fish L-1 water, respectively. D...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Li H,Ma Y,Liu Y,Wu M,Long J,Jing X,Zhou S,Yuan P,Jiang J

    更新日期:2020-10-01 00:00:00

  • Molecular cloning of rhamnose-binding lectin gene and its promoter region from snakehead Channa argus.

    abstract::Lectins are sugar-binding proteins that mediate pathogen recognition and cell-cell interactions. A rhamnose-binding lectin (RBL) gene and its promoter region have been cloned and characterized from snakehead Channa argus. From the transcription initiation site, snakehead rhamnose-binding lectin (SHL) gene extends 2,38...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Jia WZ,Shang N,Guo QL

    更新日期:2010-09-01 00:00:00

  • Intracellular mediation of GnRH action on GTH release in tilapia.

    abstract::The objective of the present study was to confirm previous results on the mediation of GnRH signal in tilapia by providing evidence from experiments in cultured pituitary cells and from perifusion experiments using a GnRH-antagonist. After 4 days in culture under identical conditions, cells taken from pituitaries of f...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Levavi-Sivan B,Yaron Z

    更新日期:1993-07-01 00:00:00

  • Effects of diet and feeding time on daily variations in plasma insulin, hepatic c-AMP and other metabolites in a teleost fish,Dicentrarchus labrax L.

    abstract::Juvenile sea bass, 1.5 years old, of mixed sex, held on long photoperiods were fed early on the photoperiod and late on the photoperiod, using different diets. Fish fed natural diets showed a daily rhythmicity of plasma insulin, liver c-AMP, plasma glucose, liver glycogen and muscle glycogen content, however, fish fed...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Perez J,Zanuy S,Carrillo M

    更新日期:1988-10-01 00:00:00

  • Spawning induces a shift in energy metabolism from glucose to lipid in rainbow trout white muscle.

    abstract::Enzymatic changes that occur in the white somatic muscle of rainbow trout (Oncorhynchus mykiss) in response to spawning were investigated, and the evenness of their distribution across the ventral-dorsal plane of this muscle was assessed. Four enzymes that are involved in energy metabolism were measured (phosphofructo...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Kiessling A,Larsson L,Kiessling KH,Lutes PB,Storebakken T,Hung SS

    更新日期:1995-12-01 00:00:00

  • The tilapia prolactin cell: A model for stimulus-secretion coupling.

    abstract::The tilapia prolactin (PRL) cell responds rapidly (10-20 min) to small physiological changes in medium osmotic pressure (OP), releasing increasing quantities of hormone as medium OP is reduced. This release is rapidly (≤ 10 min) inhibited by somatostatin (SRIF). There is now extensive evidence that tilapia PRL cell fu...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Grau EG,Helms LM

    更新日期:1989-06-01 00:00:00

  • Biotechnology applied to fish reproduction: tools for conservation.

    abstract::This review discusses the new biotechnological tools that are arising and promising for conservation and enhancement of fish production, mainly regarding the endangered and the most economically important species. Two main techniques, in particular, are available to avoid extinction of endangered fish species and to i...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章,评审


    authors: de Siqueira-Silva DH,Saito T,Dos Santos-Silva AP,da Silva Costa R,Psenicka M,Yasui GS

    更新日期:2018-12-01 00:00:00

  • High dietary lipid level alters the growth, hepatic metabolism enzyme, and anti-oxidative capacity in juvenile largemouth bass Micropterus salmoides.

    abstract::The present study was conducted to investigate the effects of high dietary lipid levels on growth, metabolism, antioxidant capacity, and immune responses of largemouth bass. Fish (initial body weight 13.38 ± 0.11 g) were fed three isonitrogenous semi-purified diets containing 5%, 10%, and 20% lipid, respectively. The ...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Zhou YL,Guo JL,Tang RJ,Ma HJ,Chen YJ,Lin SM

    更新日期:2020-02-01 00:00:00

  • Sperm quality analysis of normal season (NG) and out-season by photoperiod manipulation (PG) of male rainbow trout broodstock (Oncorhynchus mykiss).

    abstract::Sperm quality parameters in rainbow trout (Oncorhynchus mykiss) were investigated during normal season spawning (November-January) and out-season spawning (July-August) treated with artificial photoperiod manipulation. Normal spawning males (n = 15) were kept in an open concrete pond under natural condition. Out-seaso...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Momin M,Memiş D

    更新日期:2018-12-01 00:00:00

  • Effect of yeast polysaccharide on some hematologic parameter and gut morphology in channel catfish (Ictalurus punctatus).

    abstract::A study was conducted to investigate the effect of dietary yeast polysaccharides on some hematologic parameters and intestinal morphology of channel catfish. Channel catfish were fed diets containing yeast polysaccharides at 0 (control), 0.1, 0.2, or 0.3 % for 7 weeks. Each diet was provided to 10 channel catfish spec...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章,随机对照试验


    authors: Zhu H,Liu H,Yan J,Wang R,Liu L

    更新日期:2012-10-01 00:00:00

  • Annual patterns of ocular melatonin level in the female grass puffer, Takifugu alboplumbeus: possible involvement in seasonal reproductive response.

    abstract::The aim of this study was to investigate the expression patterns of ocular melatonin in the annual reproductive cycle of the female grass puffer. Spawning season of the female grass puffer is from June to July in Jeju, South Korea. Time-resolved fluoroimmunoassay revealed that levels of ocular melatonin, which show an...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Kim BH,Hur SP,Hyeon JY,Yamashina F,Takemura A,Lee YD

    更新日期:2020-06-01 00:00:00

  • Osmoregulation in the mudskipper,Boleophthalmus boddaerti I. Responses of branchial cation activated and anion stimulated adenosine triphosphatases to changes in salinity.

    abstract::The mudskipperB. boddaerti, was able to survive in waters of intermediate salinities (4-27‰). Fish submerged in dechlorinated tap water suffered 60% mortality by the fifth day while 60% of those in 100% sea-water (sw) died after the third day of exposure. After being submerged in 50% or 80% sw for 7 days, the plasma o...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Ip YK,Lee CG,Low WP,Lam TJ

    更新日期:1991-03-01 00:00:00

  • Pre-spawning water temperature affects sperm respiration and reactivation parameters in male carps.

    abstract::Concentration, ability to motility, motility during the second activation (reactivation), and endogenous respiration were studied in sperm from two experimental groups of carp males. Group 1 was maintained for 7 days at 15 degrees C (cold water (CW) group), whereas the second group was subjected to a temperature of 20...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Boryshpolets S,Dzyuba B,Drokin S

    更新日期:2009-11-01 00:00:00

  • Development of digestive enzymes in larvae of Mayan cichlid Cichlasoma urophthalmus.

    abstract::The development of digestive enzymes during the early ontogeny of the Mayan cichlid (Cichlasoma urophthalmus) was studied using biochemical and electrophoretic techniques. From yolk absorption (6 days after hatching: dah), larvae were fed Artemia nauplii until 15 dah, afterward they were fed with commercial microparti...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: López-Ramírez G,Cuenca-Soria CA,Alvarez-González CA,Tovar-Ramírez D,Ortiz-Galindo JL,Perales-García N,Márquez-Couturier G,Arias-Rodríguez L,Indy JR,Contreras-Sánchez WM,Gisbert E,Moyano FJ

    更新日期:2011-03-01 00:00:00

  • Modifications in the proteome of rainbow trout (Oncorhynchus mykiss) embryo and fry as an effect of triploidy induction.

    abstract::Two-dimensional gel electrophoresis (2-DE), matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) mass spectrometry, and database searching were used to analyze the effects of triploidization heat shock treatment on protein expression in rainbow trout eyed embryo and fry. After fertilizatio...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Babaheydari SB,Keyvanshokooh S,Dorafshan S,Johari SA

    更新日期:2017-04-01 00:00:00

  • Relationship between metabolic rate in vitro and body mass in a marine teleost, porgy Pagrus major.

    abstract::The rate of oxygen consumption of minced whole body was determined volumetrically, as an indication of metabolic rate in vitro (M in vitro ), at 20°C in porgy Pagrus major ranging from 0.0002 g (just after hatch) to 2.9 g (67 days old) in body mass. A triphasic relationship was found between M in vitro of individual f...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Oikawa S,Itazawa Y

    更新日期:1992-10-01 00:00:00

  • Influences of glycyrrhetinic acid (GA) dietary supplementation on growth, feed utilization, and expression of lipid metabolism genes in channel catfish (Ictalurus punctatus) fed a high-fat diet.

    abstract::An 8-week feeding trial was performed to test the effects of glycyrrhetinic acid (GA) on growth and some gene expression of hepatic lipid metabolism in channel catfish (initial body weight, 3.5 ± 0.02 g) fed high-fat diets. Fish were fed the control diet, high-fat diet (HFD), and HFD supplemented with 0.4, 0.8, and 1....

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Desouky HE,Jiang GZ,Zhang DD,Abasubong KP,Yuan X,Li XF,Liu WB

    更新日期:2020-04-01 00:00:00

  • Oxidative stress and antioxidant enzymes activities in the African catfish, Clarias gariepinus, experimentally challenged with Escherichia coli and Vibrio fischeri.

    abstract::The impacts of bacterial infection on cultivated fish species, African catfish, were investigated using oxidative stress biomarkers [lipid peroxidation (LPO) and protein carbonylation] and the activities of important antioxidant/detoxifying enzymes [catalase and glutathione S-transferase (GST)]. Fish were inoculated v...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Adeyemi JA

    更新日期:2014-04-01 00:00:00

  • Multiple vitellogenins in zebrafish (Danio rerio): quantitative inventory of genes, transcripts and proteins, and relation to egg quality.

    abstract::Scrutiny of the zebrafish (Danio rerio) genomic database confirmed eight functional vitellogenin (vtg) genes, each with one or two transcript variants, and the encoded Vtg polypeptides were structurally and functionally characterized in detail by in silico and experimental analyses. There were five type I (vtgs1, 4, 5...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Yilmaz O,Patinote A,Nguyen T,Bobe J

    更新日期:2018-12-01 00:00:00

  • Assessment of spermatogenesis and plasma sex steroids in a seasonal breeding teleost: a comparative study in an area of influence of a tributary, downstream from a hydroelectric power dam, Brazil.

    abstract::River damming and building of hydroelectric power plants interrupt the reproductive migration routes and change the major physicochemical parameters of water quality, with drastic consequences for populations of migratory fishes. The goal of this study was to evaluate proliferation and cell death during spermatogenesi...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Domingos FFT,Thomé RG,Arantes FP,Castro ACS,Sato Y,Bazzoli N,Rizzo E

    更新日期:2012-12-01 00:00:00

  • Isolation of the pituitary gonadotrophic α-subunit hormone of the giant amazonian fish: pirarucu (Arapaima gigas).

    abstract::The cDNAs of the α-subunit of the pituitary gonadotrophic hormones (GTHα) of fish of the order Osteoglossiformes or the superorder Osteoglossomorpha have never been sequenced. For a better understanding the phylogenetic diversity and evolution of PGHα in fish and for future biotechnological synthesis of the gonadotrop...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Faria MT,Carvalho RF,Sevilhano TC,Oliveira NA,Silva CF,Oliveira JE,Soares CR,Garcez R,Santo PR,Bartolini P

    更新日期:2013-06-01 00:00:00

  • Reversible sterilization by supplementing turmeric (Curcuma longa) powder to diets of female Pseudotropheus socolofi.

    abstract::This study was conducted to determine if diets supplemented with turmeric powder (Curcuma longa) affected the reversible sterilization of Pseudotropheus socolofi. Three experimental diets were formulated to contain 0%, 10%, and 14% turmeric powder. The fish (mean weight 13 g) were randomly divided into groups consisti...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Koca SB,Yigit NO,Ozdogan HBE,Ozmen O

    更新日期:2020-08-01 00:00:00

  • The cytochrome P450 system of atlantic salmon (Salmo salar): I. Basal properties and induction of P450 1A1 in liver of immature and mature fish.

    abstract::The major components of the cytochrome P450 (P450) system in liver microsomes of Atlantic salmon were studied using spectrophotometric, catalytic and immunochemical techniques. In juvenile fish sampled during the winter season, high basal activities of 7-ethoxyresorufin O-deethylase (EROD) were found. The Km for 7-eth...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Goksøyr A,Larsen HE

    更新日期:1991-12-01 00:00:00

  • Stimulation of acetylcholinesterase activity by triiodothyronine in the brain of Singi fish, Heteropneustes fossilis (Bloch).

    abstract::Three consecutive days of injections of triiodothyronine (T3)(0.038, 0.075, 0.15 and 1.54 nmoles/g) significantly elevated the acetylcholinesterase (AchE) activity in the brain of Singi fish, Heteropneustes fossilis (Bloch). The higher doses of 0.075, 0.15 and 1.54 nmoles of T3/g induced a greater increase in enzyme a...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: De S,Dasmahapatra AK,Medda AK

    更新日期:1993-03-01 00:00:00

  • Effects of lamprey gonadotropin-releasing hormone and analogs on steroidogenesis and spermiation in male sea lampreys.

    abstract::The biological activities of lamprey GnRH and analogs were determined in the adult male lamprey,Petromyzon marinus. Two successive injections of lamprey GnRH at 0.005, 0.065 or 0.163 μg/g body weight or [D-Ala(6),Pro(9)-NHEt] mammal GnRH at 0.05 μg/g stimulated plasma estradiol and progesterone levels in adult male la...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Sower SA

    更新日期:1989-06-01 00:00:00

  • The relationship between trace elements in fish otoliths of wild carp and hydrochemical conditions.

    abstract::The trace element composition of the fish otolith is an indicator of biomineralization. In contrast to other skeletal tissue, the otolith retains its entire original structure and does not absorb any elements after the fish dies. Because otoliths in carp degrade very slowly in the dead body, the information it provide...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章


    authors: Gao Y,Feng Q,Ren D,Qiao L,Li S

    更新日期:2010-03-01 00:00:00