Abstract：
:This study was conducted to characterize the alterations in reduced glutathione (GSH) level, catalase (CAT) activity and proteins electrophoretic patterns in response to sublethal copper (Cu) exposure in Cyprinus carpio and to determine whether these responses are related to Cu accumulation in gills, chosen as target tissue. Fish were exposed to 0.1 and 1.0 mg/l Cu for 10 and 20 days. There were increasing level of Cu in the gill with increasing concentrations of metal in the exposure medium, and with increasing duration of exposure. GSH level and CAT activity increased in fish exposed to 1.0 mg/l Cu for both exposure periods, while no change was detected at the lower Cu concentration. Electrophoretic patterns of gill proteins by sodium dodecyl sulphate gel electrophoresis (SDS-PAGE) consist of 25-, 26-, 30-, 44- and 48-kDA medium molecular weight proteins (MMP) for five bands and 64-, 72-, 90- and 101-kDA high molecular weight proteins (HMP) for four bands in both control and treatment groups. The levels of 25-, 26- and 30-kDA MMP and 72- and 90-kDA HMP increased in response to Cu exposure. The present study demonstrated that Cu caused stress in fish gills and an acclimation with induction of GSH, CAT, MMP and HMP, which were important in the protection against metal damage, was observed.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Fırat O,Kargın Fdoi
10.1007/s10695-010-9380-0subject
Has Abstractpub_date
2010-12-01 00:00:00pages
1021-8issue
4eissn
0920-1742issn
1573-5168journal_volume
36pub_type
杂志文章abstract：:This study was performed to unravel the mechanism of the beneficial action of taurine on marine teleost fish, red sea bream (Pagrus major), by analyzing the hepatic metabolism. Moreover, the ameliorative effects of the nutrient against cadmium toxicity and bioaccumulation were further evaluated. The fish were fed a di...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-016-0274-7
更新日期：2017-02-01 00:00:00
abstract：:The main indication of cimetidine is being H₂-receptor antagonist, but studies suggest that cimetidine may also act as a non-specific stimulant of cell-mediated immunity and immunomodulator. In order to determine the immunomodulatory effect of dietary intake of cimetidine in the common carp (100 ± 10 g), subjects were...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-013-9804-8
更新日期：2013-12-01 00:00:00
abstract：:Endocrine-disrupting chemicals (EDCs) can affect normal sexual differentiation in fish. Foxl2, one forkhead transcription factor, plays an important role in ovarian differentiation in the early development of the female gonad in mammals and fish. How EDCs affect Foxl2 expression is little known. In this study, we isol...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9548-2
更新日期：2012-06-01 00:00:00
abstract：:Teleosts have many spawning strategies and the hormonal control of gametogenesis is not well defined among the species or even, between sexes. To increase the knowledge of gonadotropin hormones, we studied the trend by gene expression of gonadotropin receptors in the follicles and testis at different maturity stages i...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-018-0479-z
更新日期：2018-06-01 00:00:00
abstract：:The Brazilian freshwater fish diversity is the richest in the world. Only 0.7% of all Brazilian species have had any aspect of their sperm biology addressed up to this date. The majority of the fish species described in this review migrate during the spawning season (a phenomenon known as piracema). Urbanization, poll...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章,评审
doi：10.1007/s10695-008-9240-3
更新日期：2009-03-01 00:00:00
abstract：:This study aimed to investigate the stress response of Sparus aurata specimens fed with nutraceutical aquafeed brewer's yeast (Saccharomyces cerevisiae) and spirulina (Arthrospira platensis). For that purpose, 96 (169.0 ± 2.8 g) animals were distributed randomly in 12 tanks (8 fish per tank, 4 replicates) and divided ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00677-8
更新日期：2019-12-01 00:00:00
abstract：:Juvenile sea bass, 1.5 years old, of mixed sex, held on long photoperiods were fed early on the photoperiod and late on the photoperiod, using different diets. Fish fed natural diets showed a daily rhythmicity of plasma insulin, liver c-AMP, plasma glucose, liver glycogen and muscle glycogen content, however, fish fed...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01874796
更新日期：1988-10-01 00:00:00
abstract：:Copper is one of the major heavy metal pollutants found in the aquatic environment. Therefore, it is important for determining the genes that play a key role in copper metabolism in aquatic organisms. This study, thus, aimed to identify a new copper-inducible gene in swordtail fish, Xiphophorus helleri. Using ACP-base...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-007-9153-6
更新日期：2008-06-01 00:00:00
abstract：:The dmrt (doublesex and mab-3 related transcription factor) gene family comprises several transcription factors that share a conserved DM domain. Dmrt1 is considered to be involved in sexual development, but the precise function of other family members is unclear. In this study, we isolated genomic DNA and cDNA sequen...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-008-9267-5
更新日期：2009-08-01 00:00:00
abstract：:This study was conducted to determine if diets supplemented with turmeric powder (Curcuma longa) affected the reversible sterilization of Pseudotropheus socolofi. Three experimental diets were formulated to contain 0%, 10%, and 14% turmeric powder. The fish (mean weight 13 g) were randomly divided into groups consisti...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-020-00781-0
更新日期：2020-08-01 00:00:00
abstract：:The aim of this study was to use more accurate techniques to investigate the effects of cryoprotectants (CPAs) and extenders on post-thaw sperm quality of Brycon orbignyanus and Prochilodus lineatus. Six freezing media comprising the combination of three CPAs (DMSO, methanol and methyl glycol) and two extenders (BTS a...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-014-0016-7
更新日期：2015-02-01 00:00:00
abstract：:The antioxidative role of cerium was investigated in the liver of silver crucian carp injected with lead. The fish were intraperitoneally injected with 10, 20, or 30 mg/kg wet weight PbCl(2). After a 14-day period of incubation, 35 animals were injected with a solution of 1.5 mg/kg wet weight CeCl(3). After 42 days, t...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-008-9301-7
更新日期：2010-09-01 00:00:00
abstract：:The aim of the present study was to explore the effect of waterborne zinc (control, 0.85, 2.20, 3.10 mg/l, respectively) exposure on lipid deposition and metabolism in the hepatopancreas and muscle of grass carp Ctenopharyngodon idella. The lipid content, Zn accumulation, and the activities and expression levels of se...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-016-0200-z
更新日期：2016-08-01 00:00:00
abstract：:European catfish (Silurus glanis) is a commercially important freshwater fish originating from Eastern Europe. The objective of this study was to examine the short-term storage of its eggs especially in relation to maintaining a low level of malformation in newly hatched fry. The eggs from freshly spawned individuals ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-020-00902-9
更新日期：2020-11-26 00:00:00
abstract：:Using a tame animal, the impact of otter (Lutra lutra) disturbance on over-wintering carp (Cyprinus carpio) was monitored in two experiments, 133 and 140 days, respectively, over two consecutive winters (November-April). The level of stress in over-wintering carp exposed to various intensities of disturbance by otters...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-007-9180-3
更新日期：2008-09-01 00:00:00
abstract：:The incorporation and metabolism of (n-3) and (n-6) polyunsaturated fatty acids (PUFA) supplemented to growing cultures were studied in Atlantic salmon (AS) cells. A fatty acid concentration of 25 μM considerably altered the fatty acid composition of AS cells without increasing the neutral lipid content of the cells o...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00003426
更新日期：1990-07-01 00:00:00
abstract：:The adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL)-mediated lipolysis play important roles in lipid catabolism. ATGL is considered the central rate-limiting enzyme in the mobilization of fatty acids in mammals. Currently, severe fat accumulation has been commonly detected in farmed fish globally...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-020-00904-7
更新日期：2020-11-27 00:00:00
abstract：:An embryonic stem (ES)-like cell culture system RESC from a commercially important freshwater carp, Labeo rohita, was developed using blastula stage embryos. The cells were cultured in Leibovitz-15 (L-15) medium in gelatin-coated cell culture flask supplemented with 15 % fetal bovine serum along with 10 ng ml(-1) basi...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-012-9674-5
更新日期：2012-12-01 00:00:00
abstract：:An immunohistochemical study of the sturgeon (Acipenser baeri) pituitary was undertaken using antisera directed against hormones from various classes of vertebrates, including the only pituitary hormone available from sturgeon, gonadotrophin. A positive reaction was obtained after application of antisera towards the f...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01875699
更新日期：1988-07-01 00:00:00
abstract：:Aryl hydrocarbon receptor (AhR) agonists are known to cause lethal cardiovascular deformities in fish after developmental exposure. Acute adult fish toxicity of AhR agonists is thought to be minimal, but limited evidence suggests sublethal effects may also involve the cardiac system in fish. In the present study, adul...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-014-9982-z
更新日期：2015-02-01 00:00:00
abstract：:Calcium depletion/replacement studies were carried out to examine the role of calcium in contraction of trout intestinal smoot muscle in vitro. Three chemically distinct calcium channel blockers were used to determine whether voltage operated calcium channels (VOCs) were involved in calcium entry with either agonist o...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00003410
更新日期：1990-11-01 00:00:00
abstract：:Microcystin-LR (MCLR), one of the most popular microcystins (MCs) found in many field water bodies around the world, poses great health risks to animals and humans. In the present study, healthy common carp (initial weight 24.8 ± 2.3 g) were randomly assigned to five groups. Group I was fed on normal diet as control. ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10695-017-0354-3
更新日期：2017-08-01 00:00:00
abstract：:The effects of different environmental salinities (0, 12, 40, and 55 ppt) on pepsinogen 2 (pga2), trypsinogen 2 (try2), chymotrypsinogen (ctr), and pancreatic alpha-amylase (amy2a) gene expression, and on the total activities of their corresponding enzymes, were assessed in Chelon labrosus juveniles, after their corre...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章,评审
doi：10.1007/s10695-017-0440-6
更新日期：2018-02-01 00:00:00
abstract：:Gilthead sea bream larvae (Sparus aurata) aged 47 days post hatching (dph) (11.6-12.8 mg in wet weight) were exposed to several sublethal concentrations of Cd(2+) (0.1, 5 and 10 mg/L) during 6 days in order to investigate the effects of this heavy metal on the expression of selected genes involved in detoxification (m...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-012-9727-9
更新日期：2013-06-01 00:00:00
abstract：:To investigate the effect of water temperature on gamete maturation and gamete quality, European grayling (Thymalus thymallus) were held under different temperature regimes prior to spawning. Maturation of males and females and their gamete quality depended strongly on temperature regime. The highest percentages of ma...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9526-8
更新日期：2012-04-01 00:00:00
abstract：:In the catfish Heteropneustes fossilis, the anterior kidney is a hemopoietic tissue which surrounds the adrenal homologues, interrenal (IR) and chromaffin tissues corresponding to the adrenal cortical and adrenal medulla of higher mammals. The IR tissue is arranged in cell cords around the posterior cardinal vein (PCV...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-016-0218-2
更新日期：2016-10-01 00:00:00
abstract：:The cDNAs of the α-subunit of the pituitary gonadotrophic hormones (GTHα) of fish of the order Osteoglossiformes or the superorder Osteoglossomorpha have never been sequenced. For a better understanding the phylogenetic diversity and evolution of PGHα in fish and for future biotechnological synthesis of the gonadotrop...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-012-9730-1
更新日期：2013-06-01 00:00:00
abstract：:In this study, for the first time, both neuropeptides isotocin (IT) and arginine vasotocin (AVT) have been identified and measured in urophysis, the neurohaemal organ of the caudal neurosecretory system of teleost fish. So far, AVT, but not IT, was quantified by radioimmunoassay (RIA) in urophysis of several fish spec...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-012-9746-6
更新日期：2013-08-01 00:00:00
abstract：:A 2 × 2 × 2 factorial experiment was conducted to delineate the main effect of water soaking of plant ingredients, phytase, cellulase, and their interactions on the growth and digestive enzyme activities of Labeo rohita fingerlings. Two basal diets were prepared using water-soaked (S) or unsoaked (US) plant-based ingr...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9511-2
更新日期：2012-04-01 00:00:00
abstract：:Two-dimensional gel electrophoresis (2-DE), matrix-assisted laser desorption/ionization tandem time-of-flight (MALDI-TOF/TOF) mass spectrometry, and database searching were used to analyze the effects of triploidization heat shock treatment on protein expression in rainbow trout eyed embryo and fry. After fertilizatio...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-016-0312-5
更新日期：2017-04-01 00:00:00
