Abstract：
:Copper is one of the major heavy metal pollutants found in the aquatic environment. Therefore, it is important for determining the genes that play a key role in copper metabolism in aquatic organisms. This study, thus, aimed to identify a new copper-inducible gene in swordtail fish, Xiphophorus helleri. Using ACP-based RT-PCR coupled with RLM-RACE, we cloned Wap65, a mammalian homologue of hemopexin gene. The gene exhibits high identity at amino acid levels with the Wap65 gene of other fish species (42-68%) and mammalian hemopexin gene (35-37%). In addition, ten cysteine and two histidine residues are conserved in the swordtail fish Wap65 gene. These cysteine residues are vital for structural integrity, and histidine residues provide high binding affinity towards heme. As revealed by RT-PCR, the gene was upregulated in swordtail fish that were exposed to copper in a dose- and time-dependent manner. Therefore, the identification of Wap65, a mammalian homologue of hemopexin, as a new copper-inducible gene will provide greater insight into the role of this gene in copper metabolism.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Aliza D,Ismail IS,Kuah MK,Shu-Chien AC,Tengku Muhammad TSdoi
10.1007/s10695-007-9153-6subject
Has Abstractpub_date
2008-06-01 00:00:00pages
129-38issue
2eissn
0920-1742issn
1573-5168journal_volume
34pub_type
杂志文章abstract：:The present study investigates the nephrotoxic effects of two acute doses of silver nanoparticles (AgNPs) and silver nitrate (AgNO3) on the African catfish, Clarias gariepinus, using biochemical, histochemical, and histopathological changes as biomarkers. AgNP-induced impacts were recorded in some of these characteris...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00686-7
更新日期：2019-12-01 00:00:00
abstract：:Muscle accretion is affected by the difference between protein synthesis and its degradation. Studies on different species revealed that muscle proteolysis is mediated by different pathways including the ubiquitin-proteasome pathway in which the ubiquitin protein ligases play an important role. These muscle atrophy as...
journal_title：Fish physiology and biochemistry
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abstract：:The extensive use of pharmaceuticals in human and veterinary medicine may enter the aquatic environment and pose a serious threat to non-target aquatic organisms like fish. In this study, Indian major carp Cirrhinus mrigala was exposed to different concentrations (1, 10 and 100 μg L⁻¹) of most commonly used pharmaceut...
journal_title：Fish physiology and biochemistry
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doi：10.1007/s10695-013-9797-3
更新日期：2013-12-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章,评审
doi：10.1007/s10695-008-9252-z
更新日期：2009-03-01 00:00:00
abstract：:The impacts of bacterial infection on cultivated fish species, African catfish, were investigated using oxidative stress biomarkers [lipid peroxidation (LPO) and protein carbonylation] and the activities of important antioxidant/detoxifying enzymes [catalase and glutathione S-transferase (GST)]. Fish were inoculated v...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-013-9847-x
更新日期：2014-04-01 00:00:00
abstract：:The tissue distribution pattern of lipid is highly diverse among different fish species. Tiger puffer has a special lipid storage pattern, storing lipid predominantly in liver. In order to better understand the lipid physiology in fish storing lipid in liver, the present study preliminarily investigated the tissue dis...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-020-00815-7
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-014-9967-y
更新日期：2014-12-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
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更新日期：2020-06-01 00:00:00
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journal_title：Fish physiology and biochemistry
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doi：10.1007/s10695-011-9502-3
更新日期：2011-12-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-014-9938-3
更新日期：2014-10-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10695-017-0354-3
更新日期：2017-08-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF02265154
更新日期：1991-12-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004605
更新日期：1993-04-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-020-00902-9
更新日期：2020-11-26 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0110-5
更新日期：2015-12-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-006-7134-9
更新日期：2006-03-01 00:00:00
abstract：:The large yellow croaker (Larimichthys crocea) is a marine fish that is economically important to Chinese fisheries, and its reproductive and developmental biology have been extensively investigated. However, the molecular mechanism of oogenesis in L. crocea is not clear. Here, we investigated the multiple vitellogeni...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-018-0569-y
更新日期：2019-06-01 00:00:00
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更新日期：2012-06-01 00:00:00
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更新日期：2014-10-01 00:00:00
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journal_title：Fish physiology and biochemistry
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更新日期：2010-09-01 00:00:00
abstract：:Concentration, ability to motility, motility during the second activation (reactivation), and endogenous respiration were studied in sperm from two experimental groups of carp males. Group 1 was maintained for 7 days at 15 degrees C (cold water (CW) group), whereas the second group was subjected to a temperature of 20...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-008-9292-4
更新日期：2009-11-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
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更新日期：2020-11-26 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9526-8
更新日期：2012-04-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-010-9407-6
更新日期：2010-12-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
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更新日期：1993-12-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
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更新日期：2020-08-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-014-9982-z
更新日期：2015-02-01 00:00:00
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journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004327
更新日期：1994-12-01 00:00:00
