Abstract：
:Although there are almost thirty-thousand species of fish living in a great variety of habitats and utilizing vast reproductive strategies, our knowledge of morphofunctional and quantitative aspects of testis structure and spermatogenesis is still incipient for this group of vertebrates. In this review, we discuss aspects that are important to better understanding of testis structure and function, and of the development of germ cells (GC) during spermatogenesis. To achieve this, we have recently completed a number of studies presenting morphometric and functional data related to the numbers of GC and Sertoli cells (SC) per each type of spermatogenic cyst, the number of spermatogonial generations, the SC efficiency, and the magnitude of GC loss that normally occurs during spermatogenesis. We also investigated SC proliferation and the relationship of this important event to early spermatogenic cysts. The available data strongly suggest that SC proliferation in sexually mature tilapia is the primary factor responsible for the increase in testis size and for determination of the magnitude of sperm production. The influence of temperature on the duration of spermatogenesis in tilapia was also evaluated and we have used this knowledge to deplete endogenous spermatogenesis in this teleost, in order to develop an experimental system for GC transplantation. This exciting technique results in new possibilities for investigation of spermatogenesis and spermatogonial stem cell biology, creating also an entirely new and promising scenario in biotechnology-transgenic animal production and the preservation of the genetic stocks of valuable animals or endangered species.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Nóbrega RH,Batlouni SR,França LRdoi
10.1007/s10695-008-9252-zsubject
Has Abstractpub_date
2009-03-01 00:00:00pages
197-206issue
1eissn
0920-1742issn
1573-5168journal_volume
35pub_type
杂志文章,评审abstract：:Currently, the larviculture of many marine fish species with small-sized larvae depends for a short time after hatching, on the supply of high-quality live zooplankton to ensure high survival and growth rates. During the last few decades, the research community has made great efforts to develop artificial diets, which...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-018-0528-7
更新日期：2018-10-01 00:00:00
abstract：:The purpose of the current study was to examine seasonal changes in seawater tolerance and growth performance of anadromous Arctic charr (Salvelinus alpinus L.) held at the same temperature (8°C) during winter and summer. Charr (20-27 cm), previously reared in freshwater under natural photoperiod, were transferred eit...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004413
更新日期：1993-12-01 00:00:00
abstract：:Using a tame animal, the impact of otter (Lutra lutra) disturbance on over-wintering carp (Cyprinus carpio) was monitored in two experiments, 133 and 140 days, respectively, over two consecutive winters (November-April). The level of stress in over-wintering carp exposed to various intensities of disturbance by otters...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-007-9180-3
更新日期：2008-09-01 00:00:00
abstract：:The Indian major carp, mrigal (Cirrhinus mrigala), is a bottom-dwelling fish that can survive hypoxic episodes in its natural environment. We hypothesise that it can better survive hypoxic conditions by altering metabolic responses through GABA (Gamma-aminobutyric acid) supplementation. In the first experiment, the hy...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00708-4
更新日期：2020-02-01 00:00:00
abstract：:The questions addressed in this study were: 1) whether insulin added to the incubation medium can down-regulate (125)I insulin binding to isolated hepatocytes of Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss); 2) whether quantitative assessment of insulin processing can be made on isolated fish ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004590
更新日期：1993-07-01 00:00:00
abstract：:A fully developed stomach, characterized by the secretion of pepsinogens and chlorhydric acid, is vital for digestion and survival of fish larvae. To further understand the functional development of the stomach of mandarin fish (Siniperca chuatsi) during early ontogeny, the temporal and spatial expression of pepsinoge...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-012-9748-4
更新日期：2013-08-01 00:00:00
abstract：:In most sciaenids, males possess sonic muscles and produce sound through the contraction of these muscles and amplification of the swim bladder. The sonic muscles in some fishes exhibit seasonal changes in size. For example, they are hypertrophic in the spawning season, and atrophic in the non-spawning months. The pro...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9502-3
更新日期：2011-12-01 00:00:00
abstract：:The incorporation and metabolism of (n-3) and (n-6) polyunsaturated fatty acids (PUFA) supplemented to growing cultures were studied in Atlantic salmon (AS) cells. A fatty acid concentration of 25 μM considerably altered the fatty acid composition of AS cells without increasing the neutral lipid content of the cells o...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00003426
更新日期：1990-07-01 00:00:00
abstract：:Groupers are widely distributed throughout the tropical and subtropical waters of the world and are regarded as a favourite marine food fish. However, their large-scale aquaculture has been hindered by the rarity of natural males. Being protogynous hermaphrodites, groupers have been considered as study model for devel...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-008-9219-0
更新日期：2010-06-01 00:00:00
abstract：:Continuous fin cell lines from diploid, triploid, and tetraploid oriental weatherfish, Misgurnus anguillicaudatus, were established and characterized. The cell lines, designated DIMF, TRMF, and TEMF, respectively, were subcultured more than 80 times since initiation in October 2012 and were preserved at the China Cent...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0036-y
更新日期：2015-06-01 00:00:00
abstract：:Fish suffer from anemia and hypovolemic hypotensive shock after in vivo exposure with microcystins.However, except for in vivo causes for anemia and hypotension, an in vitro study of fish erythrocytes exposed to MC is necessary. For a better understanding of hematology toxicity of MC, the main aim of the present study...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9572-2
更新日期：2012-06-01 00:00:00
abstract：:Iron is one the most abundant metals on the earth being essential for living organisms even though its free form can be toxic. The overload of this metal may be related with some disorders, like Alzheimer and Parkinson diseases, and hemochromatosis in the liver. The aim of the present study was to evaluate the effects...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-010-9459-7
更新日期：2011-09-01 00:00:00
abstract：:In this study, for the first time, both neuropeptides isotocin (IT) and arginine vasotocin (AVT) have been identified and measured in urophysis, the neurohaemal organ of the caudal neurosecretory system of teleost fish. So far, AVT, but not IT, was quantified by radioimmunoassay (RIA) in urophysis of several fish spec...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-012-9746-6
更新日期：2013-08-01 00:00:00
abstract：:Drinking in freshwater juvenile salmon was investigated in response to vasodilation by sodium nitroprusside (SNP), a nitric oxide donor, which significantly increased blood vessel diameter in Atlantic salmon alevins. Atlantic salmon fry (1-3 g), as previously shown, drank at a significant rate in fresh water which dou...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01874839
更新日期：1996-02-01 00:00:00
abstract：:The peptide YYb (PYYb) is a fish-specific peptide belonging to the neuropeptide Y (NPY) family. In the present study, the full-length cDNA sequence and genomic structure of PYYb (gcPYYb) from Ctenopharyngodon idellus have been isolated and characterized. The gcPYYb gene consists of three exons interspaced by two intro...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-013-9822-6
更新日期：2014-02-01 00:00:00
abstract：:A 90-day randomized feeding experiment was performed to assess the effects of dietary cobalt (Co) supplementation on the growth performance, muscle composition, status of iron and manganese in the muscle as well as the expression of growth-related genes in the muscle (myoblast determination protein 1 homolog (MyoD) an...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00723-5
更新日期：2020-02-01 00:00:00
abstract：:Fish metabolic allostatic dynamics, when animal present physiological modifications that can be strategies to survive, are important for promoting changes to ensure whole body self-protection and survival in chronic states of stress. To determine the impact of sequential stressors on pacu (Piaractus mesopotamicus), fi...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-020-00789-6
更新日期：2020-08-01 00:00:00
abstract：:Plasma levels of L-thyroxine (T4) and 3,5,3'-triiodo-L-thyronine (T3) and the percentage of plasma T4 and T3 present in the free (dialyzable) form (%FT4 and %FT3) were measured in 16 species (11 families) of tropical marine teleosts from an inshore Barbados reef. Mean plasma T4 varied from 0.2 ng/ml to 42 ng/ml; mean ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF02180414
更新日期：1987-05-01 00:00:00
abstract：:Copper is one of the major heavy metal pollutants found in the aquatic environment. Therefore, it is important for determining the genes that play a key role in copper metabolism in aquatic organisms. This study, thus, aimed to identify a new copper-inducible gene in swordtail fish, Xiphophorus helleri. Using ACP-base...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-007-9153-6
更新日期：2008-06-01 00:00:00
abstract：:Prolactin (PRL) is one of the most versatile hormones found in the pituitary of vertebrates and exerts its actions through binding to a specific PRL receptor (PRLR). Here we describe the cloning and characterization of a second prolactin receptor (ntPRLR2), isolated from the ovary of Nile tilapia (Oreochromis niloticu...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-009-9355-1
更新日期：2010-06-01 00:00:00
abstract：:The biological activities of lamprey GnRH and analogs were determined in the adult male lamprey,Petromyzon marinus. Two successive injections of lamprey GnRH at 0.005, 0.065 or 0.163 μg/g body weight or [D-Ala(6),Pro(9)-NHEt] mammal GnRH at 0.05 μg/g stimulated plasma estradiol and progesterone levels in adult male la...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004695
更新日期：1989-06-01 00:00:00
abstract：:Serum thyroid hormone concentrations were measured during the seven stages of metamorphosis (1-7) of the southern hemisphere lamprey, Geotria australis. The respective mean concentrations ± SEM of serum thyroxine (T4) and triiodothyronine (T3) fell from 31.73 ± 4.09 and 5.06 ± 0.70 nM in large ammocoetes sampled in Fe...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004444
更新日期：1990-03-01 00:00:00
abstract：:Lectins are sugar-binding proteins that mediate pathogen recognition and cell-cell interactions. A rhamnose-binding lectin (RBL) gene and its promoter region have been cloned and characterized from snakehead Channa argus. From the transcription initiation site, snakehead rhamnose-binding lectin (SHL) gene extends 2,38...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-009-9315-9
更新日期：2010-09-01 00:00:00
abstract：:The aims of the present study are to determine whether triiodothyronine (T3) and/or thyroxine (T4) in tilapia larvae is gifted through the mother, and to investigate the change profiles of thyrotropin (TSH), thyroid follicular cells and type I deiodinase (D1) gene expression following larval development. T3 and T4 con...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-014-9950-7
更新日期：2014-10-01 00:00:00
abstract：:Rainbow trout were infused continuously for 12h with epinephrine in the presence or absence of alpha-and/or beta-adrenergic blockade to characterize the specific adrenergic mechanisms involved in the control of blood acid-base status and oxygen transport capacity. Infusion of epinephrine, alone, produced a transient r...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01871745
更新日期：1988-01-01 00:00:00
abstract：:Transferrin mRNA in liver was quantified in farmed Atlantic salmon (Salmo salar) fed three different levels (310-, 65-, and 40 ppm) of dietary iron for 26 weeks. At the time of sampling, the fish fed 40 ppm iron showed symptoms of iron deficiency. Mean values of liver storage iron was reduced from 84 to 12 mg kg(-1) a...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF02112358
更新日期：1996-08-01 00:00:00
abstract：:Cytochrome P450 aromatase, which is encoded by the CYP19a gene, converts androgens to estradiol. Considerable evidence suggests that estrogens play an important role in fish reproductive process. Therefore CYP19a is an excellent candidate gene for reproductive traits. Variants in the promoter of the CYP19a gene might ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-008-9237-y
更新日期：2009-08-01 00:00:00
abstract：:The involvement of the freshwater fish gill chloride cells (CCs) in trans-branchial calcium uptake (JinCa(2+)) was investigated. This was accomplished by assessing the interspecific relationships between the apical surface area of CCs exposed to the external environment and JinCa(2+). Three species of freshwater teleo...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004482
更新日期：1992-12-01 00:00:00
abstract：:Cells from a relatively stenohaline marine species, turbot (Scophthalmus maximus) (TF) and an anadromous species, Atlantic salmon (AS) were cultured in media supplemented with NaCl to produce OPs varying from 300 to 500 mOsm kg(-1) and the direct effects of OP (salinity) on the fatty acid compositions of the main glyc...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00002456
更新日期：1995-04-01 00:00:00
abstract：:The aim of this study was to use more accurate techniques to investigate the effects of cryoprotectants (CPAs) and extenders on post-thaw sperm quality of Brycon orbignyanus and Prochilodus lineatus. Six freezing media comprising the combination of three CPAs (DMSO, methanol and methyl glycol) and two extenders (BTS a...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-014-0016-7
更新日期：2015-02-01 00:00:00
