Investigation into effects of antipsychotics on ectonucleotidase and adenosine deaminase in zebrafish brain.

Abstract:

:Antipsychotic agents are used for the treatment of psychotic symptoms in patients with several brain disorders, such as schizophrenia. Atypical and typical antipsychotics differ regarding their clinical and side-effects profile. Haloperidol is a representative typical antipsychotic drug and has potent dopamine receptor antagonistic functions; however, atypical antipsychotics have been developed and characterized an important advance in the treatment of schizophrenia and other psychotic disorders. Purine nucleotides and nucleosides, such as ATP and adenosine, constitute a ubiquitous class of extracellular signaling molecules crucial for normal functioning of the nervous system. Indirect findings suggest that changes in the purinergic system, more specifically in adenosinergic activity, could be involved in the pathophysiology of schizophrenia. We investigated the effects of typical and atypical antipsychotics on ectonucleotidase and adenosine deaminase (ADA) activities, followed by an analysis of gene expression patterns in zebrafish brain. Haloperidol treatment (9 µM) was able to decrease ATP hydrolysis (35%), whereas there were no changes in hydrolysis of ADP and AMP in brain membranes after antipsychotic exposure. Adenosine deamination in membrane fractions was inhibited (38%) after haloperidol treatment when compared to the control; however, no changes were observed in ADA soluble fractions after haloperidol exposure. Sulpiride (250 µM) and olanzapine (100 µM) did not alter ectonucleotidase and ADA activities. Haloperidol also led to a decrease in entpd2_mq, entpd3 and adal mRNA transcripts. These findings demonstrate that haloperidol is an inhibitor of NTPDase and ADA activities in zebrafish brain, suggesting that purinergic signaling may also be a target of pharmacological effects promoted by this drug.

journal_name

Fish Physiol Biochem

authors

Seibt KJ,Oliveira Rda L,Bogo MR,Senger MR,Bonan CD

doi

10.1007/s10695-015-0093-2

subject

Has Abstract

pub_date

2015-12-01 00:00:00

pages

1383-92

issue

6

eissn

0920-1742

issn

1573-5168

pii

10.1007/s10695-015-0093-2

journal_volume

41

pub_type

杂志文章
  • Modulatory role of L-carnitine against microcystin-LR-induced immunotoxicity and oxidative stress in common carp.

    abstract::Microcystin-LR (MCLR), one of the most popular microcystins (MCs) found in many field water bodies around the world, poses great health risks to animals and humans. In the present study, healthy common carp (initial weight 24.8 ± 2.3 g) were randomly assigned to five groups. Group I was fed on normal diet as control. ...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s10695-017-0354-3

    authors: Chen XM,Guo GL,Sun L,Yang QS,Wang GQ,Zhang DM

    更新日期:2017-08-01 00:00:00

  • Immunomodulatory effect of cimetidine in common carp (Cyprinus carpio L.).

    abstract::The main indication of cimetidine is being H₂-receptor antagonist, but studies suggest that cimetidine may also act as a non-specific stimulant of cell-mediated immunity and immunomodulator. In order to determine the immunomodulatory effect of dietary intake of cimetidine in the common carp (100 ± 10 g), subjects were...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-013-9804-8

    authors: Hosseinifard SM,Ahmadpour A,Mojazi Amiri B,Razeghi Mansour M,Ebrahimpour A

    更新日期:2013-12-01 00:00:00

  • Annual patterns of ocular melatonin level in the female grass puffer, Takifugu alboplumbeus: possible involvement in seasonal reproductive response.

    abstract::The aim of this study was to investigate the expression patterns of ocular melatonin in the annual reproductive cycle of the female grass puffer. Spawning season of the female grass puffer is from June to July in Jeju, South Korea. Time-resolved fluoroimmunoassay revealed that levels of ocular melatonin, which show an...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-019-00749-9

    authors: Kim BH,Hur SP,Hyeon JY,Yamashina F,Takemura A,Lee YD

    更新日期:2020-06-01 00:00:00

  • Nano-delivery of trace minerals for marine fish larvae: influence on skeletal ossification, and the expression of genes involved in intestinal transport of minerals, osteoblast differentiation, and oxidative stress response.

    abstract::Currently, the larviculture of many marine fish species with small-sized larvae depends for a short time after hatching, on the supply of high-quality live zooplankton to ensure high survival and growth rates. During the last few decades, the research community has made great efforts to develop artificial diets, which...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-018-0528-7

    authors: Terova G,Rimoldi S,Izquierdo M,Pirrone C,Ghrab W,Bernardini G

    更新日期:2018-10-01 00:00:00

  • Physiological responses of great sturgeon (Huso huso) to different concentrations of 2-phenoxyethanol as an anesthetic.

    abstract::The objective of the study was to evaluate the efficacy of 2-phenoxyethanol (2-PE) as an anesthetic in great sturgeon under two experiments. First, fish were exposed to 0.1, 0.3, 0.5, 0.7 and 0.9 mL/L 2-PE, and time to induction (deep anesthesia) and recovery from anesthesia were measured. At concentration of 0.1 mL/L...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章,随机对照试验

    doi:10.1007/s10695-012-9659-4

    authors: Shaluei F,Hedayati A,Jahanbakhshi A,Baghfalaki M

    更新日期:2012-12-01 00:00:00

  • Developmental changes in digestive enzyme activity in American shad, Alosa sapidissima, during early ontogeny.

    abstract::In order to assess the digestive physiological capacity of the American shad Alosa sapidissima and to establish feeding protocols that match larval nutritional requirements, we investigated the ontogenesis of digestive enzymes (trypsin, amylase, lipase, pepsin, alkaline phosphatase, and leucine aminopeptidase) in larv...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-016-0295-2

    authors: Gao XQ,Liu ZF,Guan CT,Huang B,Lei JL,Li J,Guo ZL,Wang YH,Hong L

    更新日期:2017-04-01 00:00:00

  • Development of digestive enzymes in larvae of Mayan cichlid Cichlasoma urophthalmus.

    abstract::The development of digestive enzymes during the early ontogeny of the Mayan cichlid (Cichlasoma urophthalmus) was studied using biochemical and electrophoretic techniques. From yolk absorption (6 days after hatching: dah), larvae were fed Artemia nauplii until 15 dah, afterward they were fed with commercial microparti...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-010-9431-6

    authors: López-Ramírez G,Cuenca-Soria CA,Alvarez-González CA,Tovar-Ramírez D,Ortiz-Galindo JL,Perales-García N,Márquez-Couturier G,Arias-Rodríguez L,Indy JR,Contreras-Sánchez WM,Gisbert E,Moyano FJ

    更新日期:2011-03-01 00:00:00

  • Effects of dietary arachidonic acid on cortisol production and gene expression in stress response in Senegalese sole (Solea senegalensis) post-larvae.

    abstract::Dietary fatty acids, particularly arachidonic acid (ARA), affect cortisol and may influence the expression of genes involved in stress response in fish. The involvement of ARA on stress, lipid, and eicosanoid metabolism genes, in Senegalese sole, was tested. Post-larvae were fed Artemia presenting graded ARA levels (0...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-013-9778-6

    authors: Martins DA,Rocha F,Castanheira F,Mendes A,Pousão-Ferreira P,Bandarra N,Coutinho J,Morais S,Yúfera M,Conceição LE,Martínez-Rodríguez G

    更新日期:2013-10-01 00:00:00

  • Development of an ES-like cell culture system (RESC) from rohu, Labeo rohita (Ham.).

    abstract::An embryonic stem (ES)-like cell culture system RESC from a commercially important freshwater carp, Labeo rohita, was developed using blastula stage embryos. The cells were cultured in Leibovitz-15 (L-15) medium in gelatin-coated cell culture flask supplemented with 15 % fetal bovine serum along with 10 ng ml(-1) basi...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-012-9674-5

    authors: Goswami M,Lakra WS,Yadav K,Jena JK

    更新日期:2012-12-01 00:00:00

  • Food intake and appetite control in a GH-transgenic zebrafish.

    abstract::The biological actions of growth hormone (GH) are pleiotropic, including growth promotion, energy mobilization, gonadal development, appetite, and social behavior. The regulatory network for GH is complex and includes many central and peripheral endocrine factors as well as that from the environment. It is known that ...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-015-0074-5

    authors: Dalmolin C,Almeida DV,Figueiredo MA,Marins LF

    更新日期:2015-10-01 00:00:00

  • Involvement of FGF9/16/20 subfamily in female germ cell development of the Nile tilapia, Oreochromis niloticus.

    abstract::Fibroblast growth factors (FGFs) have been proved to participate in a wide variety of processes, including growth, differentiation, cell proliferation, migration, sex determination and sex differentiation. The roles of FGF9/16/20 subfamily members in the gonadal development of teleost fish have not yet been reported. ...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-012-9630-4

    authors: Sun YL,Zeng S,Ye K,Yang C,Li MH,Huang BF,Sun LN,Zhou LY,Wang DS

    更新日期:2012-10-01 00:00:00

  • Control of ion transport by the sperm duct epithelium of brook trout (Salvelinus fontinalis).

    abstract::The sperm duct epithelium of brook trout (Salvelinus fontinalis), mountedin vitro in Ussing-style epithelial chambers actively absorbs Na(+) (measured as the short-circuit current, Isc) and secretes K(+) (measured using(86)Rb(+) as tracer). Dibutyryl-cyclic-adenosine monophosphate (db-cAMP) and 3-isobutyl-1-methylxant...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/BF00004725

    authors: Marshall WS,Bryson SE,Idler DR

    更新日期:1989-06-01 00:00:00

  • Development of digestive enzyme activity in larvae of spotted sand bass Paralabrax maculatofasciatus II: Electrophoretic analysis.

    abstract::The activities of several digestive enzymes during larval development of the spotted sand bass (Paralabrax maculatofasciatus) were evaluated using electrophoretic techniques. The results show the presence of three isoforms of alkaline protease from day 2 after hatching (ah) and the early appearance of one pepsin-like ...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-008-9276-4

    authors: Alvarez-González CA,Moyano-López FJ,Civera-Cerecedo R,Carrasco-Chávez V,Ortíz-Galindo JL,Nolasco-Soria H,Tovar-Ramírez D,Dumas S

    更新日期:2010-03-01 00:00:00

  • Tissue distribution of transcription for 29 lipid metabolism-related genes in Takifugu rubripes, a marine teleost storing lipid predominantly in liver.

    abstract::The tissue distribution pattern of lipid is highly diverse among different fish species. Tiger puffer has a special lipid storage pattern, storing lipid predominantly in liver. In order to better understand the lipid physiology in fish storing lipid in liver, the present study preliminarily investigated the tissue dis...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-020-00815-7

    authors: Xu H,Meng X,Jia L,Wei Y,Sun B,Liang M

    更新日期:2020-08-01 00:00:00

  • Isolation and selection of suitable reference genes for real-time PCR analyses in the skeletal muscle of the fine flounder in response to nutritional status: assessment and normalization of gene expression of growth-related genes.

    abstract::In the present study, different reference genes were isolated, and their stability in the skeletal muscle of fine flounder subjected to different nutritional states was assessed using geNorm and NormFinder. The combinations between 18S and ActB; Fau and 18S; and Fau and Tubb were chosen as the most stable gene combina...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-012-9739-5

    authors: Fuentes EN,Safian D,Valdés JA,Molina A

    更新日期:2013-08-01 00:00:00

  • Bilirubin-binding protein in the serum of spawning-migrating chum salmon,Oncorhynchus keta: Its identity with carotenoid-carrying lipoprotein.

    abstract::The serum carotenoid level gradually increased during the spawning migration of chum salmon (Oncorhynchus keta). Zeaxanthin, a yellowish carotenoid, was detected in the serum of spent males, while astaxanthin was a main carotenoid in the serum of males and females at other developmental stages. A high bilirubin level ...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/BF01875644

    authors: Ando S,Hatano M

    更新日期:1988-05-01 00:00:00

  • Detoxification and antioxidant effects of garlic and curcumin in Oreochromis niloticus injected with aflatoxin B₁ with reference to gene expression of glutathione peroxidase (GPx) by RT-PCR.

    abstract::The present study aims to investigate the effects of both garlic and curcumin through evaluating their therapeutic properties as antioxidants on liver and kidney functions, hepatic antioxidants and GPx gene expression against aflatoxicosis of O. niloticus. In total, 180 of tilapia were divided into ten groups; T1 repr...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-015-0164-4

    authors: El-Barbary MI

    更新日期:2016-04-01 00:00:00

  • Testosterone improves the transition of primary oocytes in artificial maturation eels (Anguilla japonica) by altering ovarian PTEN expression.

    abstract::In mammals, androgens appear to enhance the development of primary ovarian follicles, but PI3K (phosphoinositide 3-kinases) pathway is well recognized as one of the critical pathways in early follicular development. Roles of the PI3K were revealed by deletion of PTEN (phosphatase and tensin homolog on chromosome 10). ...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-011-9560-6

    authors: Huang YS,Chen YM,Liao PC,Lee YH,Gwo JC,Chen MC,Chang CF

    更新日期:2012-06-01 00:00:00

  • Effects of unilateral ovariectomy on recruitment and growth of follicles in the rainbow trout, Oncorhynchus mykiss.

    abstract::Virgin female rainbow trout, Oncorhynchus mykiss, were unilaterally ovariectomised at various stages of ovarian development to investigate the effect of the removal of one ovary on subsequent recruitment and growth of follicles in the remaining ovary. The right ovary was removed from groups of 12-15 fish, 12, 7 and 4 ...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/BF00003435

    authors: Tyler CR,Nagler JJ,Pottinger TG,Turner MA

    更新日期:1994-10-01 00:00:00

  • Molecular cloning of Foxl2 gene and the effects of endocrine-disrupting chemicals on its mRNA level in rare minnow, Gobiocypris rarus.

    abstract::Endocrine-disrupting chemicals (EDCs) can affect normal sexual differentiation in fish. Foxl2, one forkhead transcription factor, plays an important role in ovarian differentiation in the early development of the female gonad in mammals and fish. How EDCs affect Foxl2 expression is little known. In this study, we isol...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-011-9548-2

    authors: Wang H,Wu T,Qin F,Wang L,Wang Z

    更新日期:2012-06-01 00:00:00

  • Ontogeny of the digestive tract of Centropomus parallelus larvae.

    abstract::Development of the digestive tract and accessory glands of larvae of the fat snook Centropomus parallelus was examined under light microscopy, from hatching to 60 day post-hatching (dph). At hatching, the digestive tract is straight and composed by a cubic cell layer. The exogenous feeding starts at 3 dph, concomitant...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-015-0028-y

    authors: Teles A,Costa WM,Ammar D,Müller YM,Nazari EM,Cerqueira VR

    更新日期:2015-04-01 00:00:00

  • Erythrocyte damage of crucian carp (Carassius auratus) caused by microcystin-LR: in vitro study.

    abstract::Fish suffer from anemia and hypovolemic hypotensive shock after in vivo exposure with microcystins.However, except for in vivo causes for anemia and hypotension, an in vitro study of fish erythrocytes exposed to MC is necessary. For a better understanding of hematology toxicity of MC, the main aim of the present study...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-011-9572-2

    authors: Zhou W,Liang H,Zhang X

    更新日期:2012-06-01 00:00:00

  • The effects of starvation on fast-start escape and constant acceleration swimming performance in rose bitterling (Rhodeus ocellatus) at two acclimation temperatures.

    abstract::To investigate the effects of starvation and acclimation temperature on the escape ability of juvenile rose bitterling (Rhodeus ocellatus), we measured the fast-start escape and constant acceleration swimming performance of fish fasted for 0 (control), 1 and 2 weeks and half-lethal periods (6 or 4 weeks) at two temper...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-015-0184-0

    authors: Penghan LY,Pang X,Fu SJ

    更新日期:2016-06-01 00:00:00

  • Identification of a gonad-expression differential gene insulin-like growth factor-1 receptor (Igf1r) in the swamp eel (Monopterus albus).

    abstract::In vertebrate species, the biopotential embryonic gonad differentiation is affected by many key genes and key steroidogenic enzymes. Insulin-like growth factor-1 receptor (Igf1r) has been considered as an important sex-differentiation gene in mammals and could mediate the biological action of Igf1, an important regula...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-014-9914-y

    authors: Mei J,Yan W,Fang J,Yuan G,Chen N,He Y

    更新日期:2014-08-01 00:00:00

  • GnRH and gpcr: laser-captured single cell gene profiling.

    abstract::We have developed a novel single cell real-time quantitative PCR technique, which incorporates harvesting marker-identified single cells using laser-capture. Here, for the first time in a vertebrate species, using this innovative single cell gene profiling technique, we report the presence of G-protein coupled recepto...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-006-0018-1

    authors: Parhar IS

    更新日期:2005-04-01 00:00:00

  • Multiple vitellogenins in zebrafish (Danio rerio): quantitative inventory of genes, transcripts and proteins, and relation to egg quality.

    abstract::Scrutiny of the zebrafish (Danio rerio) genomic database confirmed eight functional vitellogenin (vtg) genes, each with one or two transcript variants, and the encoded Vtg polypeptides were structurally and functionally characterized in detail by in silico and experimental analyses. There were five type I (vtgs1, 4, 5...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-018-0524-y

    authors: Yilmaz O,Patinote A,Nguyen T,Bobe J

    更新日期:2018-12-01 00:00:00

  • Changes of vitellogenin and Lipase in captive Sterlet sturgeon Acipenser ruthenus females during previtellogenesis to early atresia.

    abstract::Plasma chemistry, lipid metabolism and vitellogenin gene expression of captive Sterlet sturgeon Acipenser ruthenus were studied in different maturity stages. A total of 32 fish were sampled, and maturity stages were identified on the basis of histological criteria and direct observation. Females were classified to fou...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-015-0189-8

    authors: Akhavan SR,Salati AP,Falahatkar B,Jalali SA

    更新日期:2016-06-01 00:00:00

  • Multiple vitellogenin genes (vtgs) in large yellow croaker (Larimichthys crocea): molecular characterization and expression pattern analysis during ovarian development.

    abstract::The large yellow croaker (Larimichthys crocea) is a marine fish that is economically important to Chinese fisheries, and its reproductive and developmental biology have been extensively investigated. However, the molecular mechanism of oogenesis in L. crocea is not clear. Here, we investigated the multiple vitellogeni...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-018-0569-y

    authors: Gao XM,Zhou Y,Zhang DD,Hou CC,Zhu JQ

    更新日期:2019-06-01 00:00:00

  • Proteolysis by juvenile sea bass (Dicentrarchus labrax) gastrointestinal enzymes as a method for the evaluation of feed proteins.

    abstract::Protein digestibility by gastrointestinal homogenates of Dicentrarchus labrax, was investigated in 5 feeds in relation to their amino acids composition and their ability to sustain growth of D. labrax post larval juveniles. Gastric proteolysis was found to contribute <25% of the total proteolytic capacity. Correlation...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/BF00003377

    authors: Lindner P,Eshell A,Kolkovski S,Tandler A,Harpaz S

    更新日期:1995-10-01 00:00:00

  • P-glycoprotein induction and its energetic costs in rainbow trout (Oncorhynchus mykiss).

    abstract::Biological organisms are constantly challenged by xenobiotics and have evolved mechanisms to reduce, neutralize, or repair toxic outcomes. The various chemical defenses all utilize energy, but their specific costs and impacts on energy budgets are currently unknown. In this study, the energetic costs associated with t...

    journal_title:Fish physiology and biochemistry

    pub_type: 杂志文章

    doi:10.1007/s10695-020-00911-8

    authors: Kennedy CJ

    更新日期:2021-01-06 00:00:00