Abstract:
:Biological organisms are constantly challenged by xenobiotics and have evolved mechanisms to reduce, neutralize, or repair toxic outcomes. The various chemical defenses all utilize energy, but their specific costs and impacts on energy budgets are currently unknown. In this study, the energetic costs associated with the induction and substrate transport of the efflux transporter P-glycoprotein (P-gp [ABCB1, MDR1]) were examined in rainbow trout. An intraperitoneal injection of the P-gp inducer clotrimazole (0, 0.1, 1.0, and 10 mg/kg) increased P-gp activity (as measured by a competitive rhodamine 123 transport assay in hepatocytes) in a dose-dependent manner reaching a maximum induction of 2.8-fold. Maximum P-gp induction occurred at 50 h post-administration with the highest dose; significant induction of P-gp activity remained elevated over constitutive values until the last sampling time point (168 h). In vitro measurements of hepatocyte respiration indicated that basal P-gp activity transporting R123 as a substrate did not significantly increase respiration rates (range 18.0 to 23.2 ng O2/min/106 cells); however, following the induction of P-gp by clotrimazole and exposure to the P-gp substrate R123, respiration rates increased significantly (3.52-fold) over baseline values. Using whole animal respirometry, it was shown that respiration rates in fish exposed to R123 only or induced with clotrimazole were not different from controls (range 1.2 to 2.1 mg O2/kg/min); however, respiration rates were significantly increased in fish with induced P-gp levels and also exposed to R123. This work indicates that basal and induced levels of P-gp activity do not incur significant energetic costs to fish; however, upon induction of P-gp and concomitant substrate exposures, energetic costs can increase and could pose challenges to organisms facing limited energy resources.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Kennedy CJdoi
10.1007/s10695-020-00911-8subject
Has Abstractpub_date
2021-01-06 00:00:00eissn
0920-1742issn
1573-5168pii
10.1007/s10695-020-00911-8pub_type
杂志文章abstract::The pineal organ of fish, through its 24h rhythmic release of melatonin, acts as a transducer of the photoperiod, influencing different physiological functions (e.g., reproduction, growth). The target sites for melatonin are poorly known in fish, especially marine species. A radioligand study was undertaken using the ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01875583
更新日期:1996-11-01 00:00:00
abstract::Individual differences in behaviour and physiological responses to stress are associated with evolutionary adaptive variation and thus raw material for evolution. In farmed animals, the interest in consistent trait associations, i.e. coping styles, has increased dramatically over the last years. However, one of limita...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-016-0231-5
更新日期:2016-10-01 00:00:00
abstract::Histological development of the digestive tract and specific activities of three digestive enzymes (trypsin, alkaline phosphatase, and pepsin) were studied in rock bream Oplegnathus fasciatus from hatching to 50 days after hatching (DAH). At hatching, the digestive tract appeared as an undifferentiated straight tube a...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-011-9507-y
更新日期:2012-04-01 00:00:00
abstract::In the present study, heat shock cognate 70 (HSC70) and inducible heat shock protein 70 (HSP70) gene of humphead snapper, Lutjanus sanguineus, were cloned by rapid amplification of cDNA ends (RACE) technique with the primers designed from the known expressed sequence tags (ESTs) identified from the subtracted cDNA lib...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-011-9487-y
更新日期:2011-12-01 00:00:00
abstract::Sperm quality parameters in rainbow trout (Oncorhynchus mykiss) were investigated during normal season spawning (November-January) and out-season spawning (July-August) treated with artificial photoperiod manipulation. Normal spawning males (n = 15) were kept in an open concrete pond under natural condition. Out-seaso...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-018-0564-3
更新日期:2018-12-01 00:00:00
abstract::Three cytosolic glutathione S-transferases [E.C. 2.5.1.18] were identified in liver of a marine flatfish, the plaice (Pleuronectes platessa) and the two major isoforms were purified to homogeneity. There was no evidence for the presence of basic transferases. The plaice GST's exhibited comparable hematin binding to ra...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00003400
更新日期:1990-11-01 00:00:00
abstract::The present study aims to investigate the effects of both garlic and curcumin through evaluating their therapeutic properties as antioxidants on liver and kidney functions, hepatic antioxidants and GPx gene expression against aflatoxicosis of O. niloticus. In total, 180 of tilapia were divided into ten groups; T1 repr...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0164-4
更新日期:2016-04-01 00:00:00
abstract::Calcium depletion/replacement studies were carried out to examine the role of calcium in contraction of trout intestinal smoot muscle in vitro. Three chemically distinct calcium channel blockers were used to determine whether voltage operated calcium channels (VOCs) were involved in calcium entry with either agonist o...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00003410
更新日期:1990-11-01 00:00:00
abstract::Connective tissue growth factor (ctgf) is involved in the proliferation, migration, adhesion of cell, and the constituent of extracellular matrix, which plays an important role in embryogenesis, angiogenesis, wound repair, and fibrosis diseases. In this study, the cDNA sequence of grass carp ctgf gene was cloned by ra...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00653-2
更新日期:2019-08-01 00:00:00
abstract::In the present study, different reference genes were isolated, and their stability in the skeletal muscle of fine flounder subjected to different nutritional states was assessed using geNorm and NormFinder. The combinations between 18S and ActB; Fau and 18S; and Fau and Tubb were chosen as the most stable gene combina...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-012-9739-5
更新日期:2013-08-01 00:00:00
abstract::A 60-day feeding trial was conducted to study the effect of exogenous enzymes (xylanase and phytase) supplementation in the non-fermented and fermented de-oiled rice bran (DORB)-based diet of Labeo rohita. Four test diets (T1-DORB-based diet, T2-fermented DORB-based diet, T3-phytase and xylanase supplemented DORB-base...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-018-0492-2
更新日期:2018-08-01 00:00:00
abstract::Testosterone (T) administration to maleAnabas testudineus significantly stimulated the activities of cytochrome oxidase, α-glycerophosphate dehydrogenase (α-GPDH) and Mg(2+) adenosine triphosphatase (Mg(2+) ATPase) and inhibited lactate dehydrogenase (LDH), cytosolic and mitochondrial malate dehydrogenases (MDH). The ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01875607
更新日期:1989-11-01 00:00:00
abstract::Vitellogenin (VTG) from spotted wolffish, Anarhichas minor, a candidate species for cold-water marine aquaculture, was purified by MgCl₂/EDTA precipitation followed by a two-step chromatographic procedure. VTG had an apparent molecular mass of 470 kDa, as determined by gel filtration, and an amino acid composition sim...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-013-9843-1
更新日期:2014-02-01 00:00:00
abstract::A fully developed stomach, characterized by the secretion of pepsinogens and chlorhydric acid, is vital for digestion and survival of fish larvae. To further understand the functional development of the stomach of mandarin fish (Siniperca chuatsi) during early ontogeny, the temporal and spatial expression of pepsinoge...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-012-9748-4
更新日期:2013-08-01 00:00:00
abstract::The study assessed the effects of surgically implanted dummy ultrasonic transmitters on physiological response of bighead carp Hypophthalmichthys nobilis in April 2011. Before the surgery, 15 blood samples were extracted randomly from 195 bighead carp samples, and then the rest of the fish were divided into three grou...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-014-9945-4
更新日期:2014-10-01 00:00:00
abstract::Evidence has recently been presented for variation in the inducibility of various 7-alkoxycoumarin-O-dealkylase activities in liver microsomes from a number of mammalian species by β-naphthoflavone (βNF). In the present study we have investigated the inducibility of hepatic microsomal 7-methoxycoumarin-O-demethylase, ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01875022
更新日期:1989-07-01 00:00:00
abstract::Variation in individual size has important consequences for a number of characteristics of fish, which can impact fish populations. The impact of fish size on recovery following exercise, however, is poorly understood, with little information existing on the recovery of ionic/osmotic variables. The goal of this study ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-011-9510-3
更新日期:2012-04-01 00:00:00
abstract::The biological activities of lamprey GnRH and analogs were determined in the adult male lamprey,Petromyzon marinus. Two successive injections of lamprey GnRH at 0.005, 0.065 or 0.163 μg/g body weight or [D-Ala(6),Pro(9)-NHEt] mammal GnRH at 0.05 μg/g stimulated plasma estradiol and progesterone levels in adult male la...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004695
更新日期:1989-06-01 00:00:00
abstract::The present study investigates the nephrotoxic effects of two acute doses of silver nanoparticles (AgNPs) and silver nitrate (AgNO3) on the African catfish, Clarias gariepinus, using biochemical, histochemical, and histopathological changes as biomarkers. AgNP-induced impacts were recorded in some of these characteris...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-019-00686-7
更新日期:2019-12-01 00:00:00
abstract::Juvenile sea bass, 1.5 years old, of mixed sex, held on long photoperiods were fed early on the photoperiod and late on the photoperiod, using different diets. Fish fed natural diets showed a daily rhythmicity of plasma insulin, liver c-AMP, plasma glucose, liver glycogen and muscle glycogen content, however, fish fed...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF01874796
更新日期:1988-10-01 00:00:00
abstract::The retinoid X receptors (RXRs) are involved in the skeletal development and other biological process such as blood vessel formation and metabolism. Partial sequences of RXRα and β genes were obtained, and their expressions were quantified on golden pompano Trachinotus ovatus at 28 days post hatching (DPH) to explore ...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0098-x
更新日期:2015-12-01 00:00:00
abstract::Gonadotropins (GTH) were isolated from pituitary glands of grass carp and their properties investigated. Acetone-dried pituitaries were extracted with ammonium acetate-ethanol and were then passed through cation exchanger, anion exchanger, and gel filtration column. Two glycoprotein gonadotropins (DE I and DE II) were...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004705
更新日期:1989-06-01 00:00:00
abstract::Studies on the digestive secretions in aquatic animals can elucidate certain aspects of their nutritive physiology. The aim of the present study was to compare the digestive lipase and phospholipase activities in ten marine species belonging to four classes following the taxonomic classification of marine organisms. A...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-012-9633-1
更新日期:2012-10-01 00:00:00
abstract::For rainbow trout Oncorhynchus mykiss, high temperature is a major abiotic stress that limits its growth and productivity. In this study, spleen macrophage respiratory burst (RB), serum superoxide dismutase (SOD), serum malondialdehyde (MDA) and mRNA expression of the SERPINH1 (HSP47) gene in different tissues (liver,...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0170-6
更新日期:2016-04-01 00:00:00
abstract::One cannot seek permission to market transgenic fish mainly because there is no field test or any basic research on technological developments for evaluating their biosafety. Infertility is a necessary adjunct to exploiting transgenic fish unless completely secure land-locked facilities are available. In this study, w...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-013-9806-6
更新日期:2013-12-01 00:00:00
abstract::The analysis of the tench growth hormone gene structure revealed a comparable organization of coding and non-coding regions than other from cyprinid species. Based on the performed mRNA and amino acid sequence alignments, gh tench is related to Asian than to European representatives of Cyprinidae family. Second aim of...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-012-9661-x
更新日期:2012-12-01 00:00:00
abstract::Serum thyroid hormone concentrations were measured during the seven stages of metamorphosis (1-7) of the southern hemisphere lamprey, Geotria australis. The respective mean concentrations ± SEM of serum thyroxine (T4) and triiodothyronine (T3) fell from 31.73 ± 4.09 and 5.06 ± 0.70 nM in large ammocoetes sampled in Fe...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004444
更新日期:1990-03-01 00:00:00
abstract::Muscle tissues contribute 34-48 % of the total body mass in fish. Proteomic analysis enables better understanding of the skeletal muscle physiology and metabolism. A proteome map reflects the general fingerprinting of the fish species and has the potential to identify novel proteins which could serve as biomarkers for...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-015-0046-9
更新日期:2015-06-01 00:00:00
abstract::Blood being a vehicle for the transport of industrial pollutants in living system, fish hematology is considered as potent biomarker. In the present study, we investigated respective sublethal effects of pulp and paper mill effluents on hematology of two commonly cultured carps, Cyprinus carpio and Ctenopharyngodon id...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/s10695-016-0258-7
更新日期:2016-12-01 00:00:00
abstract::The effects of paraquat (PQ; 1,1'-dimethyl-4,4'-bipyridylium dichloride) treatment were investigated in carp, silver carp and wels. The serum glutamic-oxalacetic transaminase (GOT; L-aspartate: 2-oxoglutarate aminotransferase, EC 2.6.1.1.) level was enhanced by 50% at 1 ppm exposure and by 100% at 10 ppm exposure in a...
journal_title:Fish physiology and biochemistry
pub_type: 杂志文章
doi:10.1007/BF00004434
更新日期:1990-01-01 00:00:00