Expression characterization and the promoter activity analysis of zebrafish hdac4.

Abstract:

:Histone deacetylase 4 (HDAC4) is an important modifier enzyme for chromatin remodeling and plays an essential role in regulating gene expression. Spatio-temporal expression spectrum revealed that zebrafish hdac4 mRNA, ubiquitously distributed in various tissues, were significantly higher at 36 hpf (hours post-fertilization) and 6 dpf (days post-fertilization) than other periods. Trichostatin A (TSA) inhibited the development of zebrafish embryos and transcription of hdac4 and mef2a (myocyte enhancer factor-2A). Moreover, five vectors containing different promoter regions of hdac4 were constructed in order to analyze promoter activity. The vector containing the region from -125 to +160 exhibited maximum luciferase activity that was approximately 30.3-fold and 58.9-fold higher than the control in two kinds of cells, respectively. By comparing the luciferase activities between the region from -302 to +30 and -698 to +30, it was suggested that the region between -698 and -302 might contain mild negative regulatory elements.

journal_name

Fish Physiol Biochem

authors

Zhu K,Wang H,Gul Y,Zhao Y,Wang W,Liu S,Wang M

doi

10.1007/s10695-011-9540-x

subject

Has Abstract

pub_date

2012-04-01 00:00:00

pages

585-93

issue

2

eissn

0920-1742

issn

1573-5168

journal_volume

38

pub_type

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