Abstract：
:An ovarian cell line was successfully developed from the juvenile ovary of Southern catfish (SCO1) (Silurus meridionalis), which was designated as SCO1. The cell line multiplied preferentially in L-15 medium with 15 % fetal bovine serum at 28 °C for more than 70 passages over a period of 420 days. SCO1 showed fibroblast-like morphology and predominantly retained a diploid karyotype of 58 chromosomes. From the gene expression patterns, SCO1 showed a characteristic of ovarian granulosa cells. After the cells were transfected with the green fluorescent protein expression vector, bright fluorescent signals could be observed in approximately 30 % cells. This cell line may be valuable for the evaluation of endocrine disruptors and studying interactions between somatic cells and germ cells.
journal_name
Fish Physiol Biochemjournal_title
Fish physiology and biochemistryauthors
Wei J,Qi W,Zhou Y,Zhang X,Dong R,Zhou L,Wang Ddoi
10.1007/s10695-014-9932-9subject
Has Abstractpub_date
2014-10-01 00:00:00pages
1383-91issue
5eissn
0920-1742issn
1573-5168journal_volume
40pub_type
杂志文章abstract：:A 90-day randomized feeding experiment was performed to assess the effects of dietary cobalt (Co) supplementation on the growth performance, muscle composition, status of iron and manganese in the muscle as well as the expression of growth-related genes in the muscle (myoblast determination protein 1 homolog (MyoD) an...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00723-5
更新日期：2020-02-01 00:00:00
abstract：:The tilapia prolactin (PRL) cell responds rapidly (10-20 min) to small physiological changes in medium osmotic pressure (OP), releasing increasing quantities of hormone as medium OP is reduced. This release is rapidly (≤ 10 min) inhibited by somatostatin (SRIF). There is now extensive evidence that tilapia PRL cell fu...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004685
更新日期：1989-06-01 00:00:00
abstract：:This study describes the digestible protein (DP) and digestible energy (DE) utilization in juvenile mulloway, and determined the requirements for maintenance. This was achieved by feeding triplicate groups of fish weighing 40 or 129 g held at two temperatures (20 or 26 degrees C), on a commercial diet (21.4 g DP mJ DE...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-008-9296-0
更新日期：2010-03-01 00:00:00
abstract：:The objective of this study was to determine the effect of two levels of inclusion of xylan oligosaccharides (XOS) extracted from corncob on growth, feed utilization, immune status and disease resistance of Mediterranean sea bass (Dicentrarchus labrax) fingerlings. Specimens of 4.75 ± 0.69 g at initial density of 2.7 ...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0110-5
更新日期：2015-12-01 00:00:00
abstract：:Muscle tissues contribute 34-48 % of the total body mass in fish. Proteomic analysis enables better understanding of the skeletal muscle physiology and metabolism. A proteome map reflects the general fingerprinting of the fish species and has the potential to identify novel proteins which could serve as biomarkers for...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0046-9
更新日期：2015-06-01 00:00:00
abstract：:Due to problems with bone deformities in farmed Atlantic salmon, there is a growing interest in the possible involvement of vitamin K in normal bone development, and sensitive biomarkers for evaluating vitamin K status are therefore needed. The vitamin K-dependent (VKD) enzyme gamma-glutamylcarboxylase (GGCX, EC 6.4.x...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-009-9335-5
更新日期：2010-09-01 00:00:00
abstract：:Prolactin (PRL) is one of the most versatile hormones found in the pituitary of vertebrates and exerts its actions through binding to a specific PRL receptor (PRLR). Here we describe the cloning and characterization of a second prolactin receptor (ntPRLR2), isolated from the ovary of Nile tilapia (Oreochromis niloticu...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-009-9355-1
更新日期：2010-06-01 00:00:00
abstract：:CDK-2, a member of the cyclin-dependent kinase family, plays an important role in many cell processes, such as cell cycle regulation, cell growth and differentiation, and cell apoptosis. Lampreys belong to the most primitive vertebrates, and there is no report about the CDK-2 gene in lampreys at present. In this study...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00683-w
更新日期：2019-12-01 00:00:00
abstract：:The development of digestive enzymes during the early ontogeny of the Mayan cichlid (Cichlasoma urophthalmus) was studied using biochemical and electrophoretic techniques. From yolk absorption (6 days after hatching: dah), larvae were fed Artemia nauplii until 15 dah, afterward they were fed with commercial microparti...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-010-9431-6
更新日期：2011-03-01 00:00:00
abstract：:The involvement of the freshwater fish gill chloride cells (CCs) in trans-branchial calcium uptake (JinCa(2+)) was investigated. This was accomplished by assessing the interspecific relationships between the apical surface area of CCs exposed to the external environment and JinCa(2+). Three species of freshwater teleo...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004482
更新日期：1992-12-01 00:00:00
abstract：:The composition of seminal plasma and metabolism of sperm of the cyprinid fishAlburnus alburnus were investigated. Statistically significant correlations were found between motility parameters and seminal fluid osmolality, pH, Na(+), K(+) and protein levels (negative correlations: % immotile spermatozoa-Na(+), K(+); p...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01875596
更新日期：1996-04-01 00:00:00
abstract：:The present study explored the protective role of dietary the extract of Angelica sinensis (EAs) on high density, CuSO4, or trichlorfon-treated Crucian carp (Carassius auratus auratus). Firstly, the study showed that the optimum density for growth and growth inhibition was 0.49 and 0.98 fish L-1 water, respectively. D...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-020-00821-9
更新日期：2020-10-01 00:00:00
abstract：:Germinal vesicle migration (GVM) and/or dissolution (GVD) were measured in goldfish oocytes, treated with 17α, 20β dihydroxyprogesterone (DHP) and other compounds considered to effect the cytoskeleton and oxidative phosphorylation,in vitro. Administration of DHP reinitiated meiotic maturation, increasing GVM and GVD i...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF02311136
更新日期：1986-09-01 00:00:00
abstract：:Oxidation of rainbow trout haemoglobin (Hb) by nitrite proceeded via an initial lag phase followed by autocatalysis when the O2 saturation of the Hb was high. At low O2 saturations, the rate of methaemoglobin (metHb) formation was strongly reduced and autocatalysis was absent. Addition of adenosine triphosphate and gu...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004376
更新日期：1993-08-01 00:00:00
abstract：:Blood being a vehicle for the transport of industrial pollutants in living system, fish hematology is considered as potent biomarker. In the present study, we investigated respective sublethal effects of pulp and paper mill effluents on hematology of two commonly cultured carps, Cyprinus carpio and Ctenopharyngodon id...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-016-0258-7
更新日期：2016-12-01 00:00:00
abstract：:Pituitary gonadotropins, follicle-stimulating hormone (FSH) and luteinizing hormone (LH), play fundamental roles in vertebrate ovarian development and function. However, there has been an increasing body of evidence that the actions of FSH and LH are mediated or modulated by a variety of locally produced peptide or pr...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-006-0026-1
更新日期：2005-04-01 00:00:00
abstract：:Three pepsinogens (PG1, PG2, and PG3) were highly purified from the stomach of freshwater fish rice field eel (Monopterus albus Zuiew) by ammonium sulfate fractionation and chromatographies on DEAE-Sephacel, Sephacryl S-200 HR. The molecular masses of the three purified PGs were all estimated as 36 kDa using SDS-PAGE....
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-010-9456-x
更新日期：2011-09-01 00:00:00
abstract：:The objective of the study was to evaluate the efficacy of 2-phenoxyethanol (2-PE) as an anesthetic in great sturgeon under two experiments. First, fish were exposed to 0.1, 0.3, 0.5, 0.7 and 0.9 mL/L 2-PE, and time to induction (deep anesthesia) and recovery from anesthesia were measured. At concentration of 0.1 mL/L...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章,随机对照试验
doi：10.1007/s10695-012-9659-4
更新日期：2012-12-01 00:00:00
abstract：:Juvenile sea bass, 1.5 years old, of mixed sex, held on long photoperiods were fed early on the photoperiod and late on the photoperiod, using different diets. Fish fed natural diets showed a daily rhythmicity of plasma insulin, liver c-AMP, plasma glucose, liver glycogen and muscle glycogen content, however, fish fed...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF01874796
更新日期：1988-10-01 00:00:00
abstract：:Teleosts have many spawning strategies and the hormonal control of gametogenesis is not well defined among the species or even, between sexes. To increase the knowledge of gonadotropin hormones, we studied the trend by gene expression of gonadotropin receptors in the follicles and testis at different maturity stages i...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-018-0479-z
更新日期：2018-06-01 00:00:00
abstract：:The present study investigates the nephrotoxic effects of two acute doses of silver nanoparticles (AgNPs) and silver nitrate (AgNO3) on the African catfish, Clarias gariepinus, using biochemical, histochemical, and histopathological changes as biomarkers. AgNP-induced impacts were recorded in some of these characteris...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-019-00686-7
更新日期：2019-12-01 00:00:00
abstract：:Three consecutive days of injections of triiodothyronine (T3)(0.038, 0.075, 0.15 and 1.54 nmoles/g) significantly elevated the acetylcholinesterase (AchE) activity in the brain of Singi fish, Heteropneustes fossilis (Bloch). The higher doses of 0.075, 0.15 and 1.54 nmoles of T3/g induced a greater increase in enzyme a...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004508
更新日期：1993-03-01 00:00:00
abstract：:Measurable in vitro hepatic monodeiodinase activity of the southern hemisphere lamprey, Geotria australis, was present only during the first 5 of the 16 month upstream spawning migration of this species. The production of T3 from T4 in vitro was pH-sensitive, and exhibited typical Michaelis-Menton kinetics. No consist...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00003372
更新日期：1990-09-01 00:00:00
abstract：:The present study aims to investigate the effects of both garlic and curcumin through evaluating their therapeutic properties as antioxidants on liver and kidney functions, hepatic antioxidants and GPx gene expression against aflatoxicosis of O. niloticus. In total, 180 of tilapia were divided into ten groups; T1 repr...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0164-4
更新日期：2016-04-01 00:00:00
abstract：:The present study evaluated, in an 18-month gilthead sea bream trial, the time course effects of genetics on individual size variation and growth compensation processes in families selected by heritable growth in the PROGENSA® breeding program. Families categorized as fast, intermediate, and slow growing had different...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-020-00899-1
更新日期：2020-11-13 00:00:00
abstract：:Ovarian cyp19a mRNA expression and P450 aromatase activity were measured in vivo in common carp Cyprinus carpio exposed to phenol for 96 h. Production of reactive oxygen species (ROS) and parameters of antioxidant defense system in serum ovary and liver of this fish after long-term phenol exposure were also studied. I...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-015-0135-9
更新日期：2016-02-01 00:00:00
abstract：:Fish intestine is very sensitive to oxidative damage. Repair of damaged enterocytes may be involved to restore normal function of fish intestine. However, studies of fish enterocyte repair are scarce. The present study aimed to investigate the potential repair role of glutamine after a H2O2 challenge. In this study, f...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-014-9938-3
更新日期：2014-10-01 00:00:00
abstract：:While in mammals, the IGF/IGFBP system is known to be involved in adipose tissue growth, the presence of such a system in fish is as yet undetermined. The present work aimed at investigating the influence of regional localization of adipose tissues and cell types on the expression of this system. Using quantitative re...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-011-9482-3
更新日期：2011-12-01 00:00:00
abstract：:In teleosts, cortisol is one of the key factors regulating the adaptation to environmental challenges, such as salinity changes. This paper compares interrenal function between fully adapted freshwater (FW) and sea water (SW) specimens of the euryhaline teleost Oreochromis mossambicus (tilapia), combining morphometric...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/BF00004289
更新日期：1995-02-01 00:00:00
abstract：:The aim of this study was to use more accurate techniques to investigate the effects of cryoprotectants (CPAs) and extenders on post-thaw sperm quality of Brycon orbignyanus and Prochilodus lineatus. Six freezing media comprising the combination of three CPAs (DMSO, methanol and methyl glycol) and two extenders (BTS a...
journal_title：Fish physiology and biochemistry
pub_type： 杂志文章
doi：10.1007/s10695-014-0016-7
更新日期：2015-02-01 00:00:00
